Q96I25 (SPF45_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 106.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Splicing factor 45 Alternative name(s): 45 kDa-splicing factor RNA-binding motif protein 17 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Reference proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 401 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia. Ref.3 |
| Subunit structure | Binds SXL. Associates with the spliceosome. |
| Subcellular location | |
| Sequence similarities | Contains 1 G-patch domain. Contains 1 RRM (RNA recognition motif) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | mRNA processing mRNA splicing |
| Cellular component | Nucleus Spliceosome |
| Ligand | RNA-binding |
| PTM | Acetylation Phosphoprotein |
| Technical term | 3D-structure Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processingInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | nucleus Inferred from direct assay. Source: HPA spliceosomal complexInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular_function | RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 401 | 401 | Splicing factor 45 | PRO_0000081903 | ||||||||||||||||||||
Regions | ||||||||||||||||||||||||
| Domain | 235 – 283 | 49 | G-patch | |||||||||||||||||||||
| Domain | 306 – 385 | 80 | RRM | |||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||
| Modified residue | 21 | 1 | N6-acetyllysine Ref.9 | |||||||||||||||||||||
| Modified residue | 71 | 1 | Phosphothreonine Ref.4 Ref.12 | |||||||||||||||||||||
| Modified residue | 155 | 1 | Phosphoserine Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.12 | |||||||||||||||||||||
| Modified residue | 169 | 1 | Phosphoserine Ref.6 Ref.10 | |||||||||||||||||||||
| Modified residue | 222 | 1 | Phosphoserine Ref.10 Ref.12 | |||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||
| Sequence conflict | 214 | 1 | Y → H in AAH09064. Ref.1 | |||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||
| Beta strand | 305 – 313 | 9 | ||||||||||||||||||||||
| Helix | 323 – 329 | 7 | ||||||||||||||||||||||
| Helix | 330 – 333 | 4 | ||||||||||||||||||||||
| Beta strand | 336 – 343 | 8 | ||||||||||||||||||||||
| Turn | 349 – 351 | 3 | ||||||||||||||||||||||
| Beta strand | 352 – 361 | 10 | ||||||||||||||||||||||
| Helix | 362 – 372 | 11 | ||||||||||||||||||||||
| Beta strand | 383 – 386 | 4 | ||||||||||||||||||||||
| Helix | 389 – 393 | 5 | ||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye, Testis and Uterus. |
| [2] | "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex." Neubauer G., King A., Rappsilber J., Calvio C., Watson M., Ajuh P., Sleeman J., Lamond A.I., Mann M. Nat. Genet. 20:46-50(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY, SUBCELLULAR LOCATION, INTERACTION WITH THE SPLICEOSOME. |
| [3] | "Splicing regulation at the second catalytic step by Sex-lethal involves 3' splice site recognition by SPF45." Lallena M.J., Chalmers K.J., Llamazares S., Lamond A.I., Valcarcel J. Cell 109:285-296(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
| [4] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-71 AND SER-155, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [5] | "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment." Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J. J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155, MASS SPECTROMETRY. Tissue: T-cell. |
| [6] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155 AND SER-169, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [7] | "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155, MASS SPECTROMETRY. Tissue: Liver. |
| [8] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155, MASS SPECTROMETRY. Tissue: Leukemic T-cell. |
| [9] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-21, MASS SPECTROMETRY. |
| [10] | "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-169 AND SER-222, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [11] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| [12] | "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-71; SER-155 AND SER-222, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | BC007871 mRNA. Translation: AAH07871.1. BC009064 mRNA. Translation: AAH09064.1. BC039322 mRNA. Translation: AAH39322.1. | ||||||||||||||||||
| IPI | IPI00176706. | ||||||||||||||||||
| RefSeq | NP_001139019.1. NM_001145547.1. NP_116294.1. NM_032905.4. | ||||||||||||||||||
| UniGene | Hs.498548. | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | Q96I25. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| DIP | DIP-29409N. | ||||||||||||||||||
| IntAct | Q96I25. 8 interactions. | ||||||||||||||||||
| MINT | MINT-3053773. | ||||||||||||||||||
| STRING | 9606.ENSP00000369218. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | Q96I25. | ||||||||||||||||||
Polymorphism databases | |||||||||||||||||||
| DMDM | 34925383. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PaxDb | Q96I25. | ||||||||||||||||||
| PeptideAtlas | Q96I25. | ||||||||||||||||||
| PRIDE | Q96I25. | ||||||||||||||||||
Protocols and materials databases | |||||||||||||||||||
| DNASU | 84991. | ||||||||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENST00000379888; ENSP00000369218; ENSG00000134453. ENST00000446108; ENSP00000388638; ENSG00000134453. | ||||||||||||||||||
| GeneID | 84991. | ||||||||||||||||||
| KEGG | hsa:84991. | ||||||||||||||||||
| UCSC | uc001ijb.3. human. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| CTD | 84991. | ||||||||||||||||||
| GeneCards | GC10P006130. | ||||||||||||||||||
| HGNC | HGNC:16944. RBM17. | ||||||||||||||||||
| HPA | HPA037477. | ||||||||||||||||||
| MIM | 606935. gene. | ||||||||||||||||||
| neXtProt | NX_Q96I25. | ||||||||||||||||||
| PharmGKB | PA134860993. | ||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| eggNOG | NOG317167. | ||||||||||||||||||
| HOGENOM | HOG000231726. | ||||||||||||||||||
| HOVERGEN | HBG059363. | ||||||||||||||||||
| InParanoid | Q96I25. | ||||||||||||||||||
| KO | K12840. | ||||||||||||||||||
| OMA | IPPPVYD. | ||||||||||||||||||
| OrthoDB | EOG48SGTF. | ||||||||||||||||||
| PhylomeDB | Q96I25. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | Q96I25. | ||||||||||||||||||
| Bgee | Q96I25. | ||||||||||||||||||
| CleanEx | HS_RBM17. | ||||||||||||||||||
| Genevestigator | Q96I25. | ||||||||||||||||||
| GermOnline | ENSG00000134453. Homo sapiens. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| Gene3D | 3.30.70.330. 1 hit. | ||||||||||||||||||
| InterPro | IPR000467. G_patch_dom. IPR012677. Nucleotide-bd_a/b_plait. IPR003954. RRM_dom_euk. IPR016967. Splicing_factor_SPF45. [Graphical view] | ||||||||||||||||||
| Pfam | PF01585. G-patch. 1 hit. [Graphical view] | ||||||||||||||||||
| PIRSF | PIRSF031066. Splicing_factor_SPF45. 1 hit. | ||||||||||||||||||
| SMART | SM00443. G_patch. 1 hit. SM00361. RRM_1. 1 hit. [Graphical view] | ||||||||||||||||||
| PROSITE | PS50174. G_PATCH. 1 hit. PS50102. RRM. False negative. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other | |||||||||||||||||||
| EvolutionaryTrace | Q96I25. | ||||||||||||||||||
| GenomeRNAi | 84991. | ||||||||||||||||||
| NextBio | 75577. | ||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||
Entry information
| Entry name | SPF45_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q96I25 Secondary accession number(s): Q96GY6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 10 Human chromosome 10: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with
