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Protein

Selenocysteine lyase

Gene

SCLY

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium.By similarity

Catalytic activityi

L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei388S-selanylcysteine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular amino acid metabolic process Source: ProtInc
  • selenium compound metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciZFISH:HS05620-MONOMER.
ReactomeiR-HSA-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.

Names & Taxonomyi

Protein namesi
Recommended name:
Selenocysteine lyase (EC:4.4.1.16)
Short name:
hSCL
Gene namesi
Name:SCLY
Synonyms:SCL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:18161. SCLY.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi51540.
OpenTargetsiENSG00000132330.
PharmGKBiPA134979359.

Polymorphism and mutation databases

BioMutaiSCLY.
DMDMi527504087.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003170121 – 445Selenocysteine lyaseAdd BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei129PhosphoserineCombined sources1
Modified residuei259N6-(pyridoxal phosphate)lysine1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96I15.
MaxQBiQ96I15.
PaxDbiQ96I15.
PeptideAtlasiQ96I15.
PRIDEiQ96I15.

PTM databases

iPTMnetiQ96I15.
PhosphoSitePlusiQ96I15.

Expressioni

Inductioni

Up-regulated in acute glomerulonephritis. Regulated by JUN/AP-1.1 Publication

Gene expression databases

BgeeiENSG00000132330.
CleanExiHS_SCLY.
ExpressionAtlasiQ96I15. baseline and differential.
GenevisibleiQ96I15. HS.

Organism-specific databases

HPAiHPA020190.
HPA055760.
HPA062822.

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TERF2IPQ9NYB02EBI-2823066,EBI-750109

Protein-protein interaction databases

BioGridi119599. 32 interactors.
IntActiQ96I15. 10 interactors.
STRINGi9606.ENSP00000254663.

Structurei

Secondary structure

1445
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni36 – 38Combined sources3
Helixi44 – 56Combined sources13
Helixi66 – 85Combined sources20
Helixi90 – 92Combined sources3
Beta strandi93 – 97Combined sources5
Helixi99 – 118Combined sources20
Beta strandi137 – 141Combined sources5
Helixi146 – 157Combined sources12
Beta strandi162 – 166Combined sources5
Turni170 – 172Combined sources3
Helixi177 – 182Combined sources6
Beta strandi188 – 192Combined sources5
Turni198 – 200Combined sources3
Helixi206 – 222Combined sources17
Beta strandi229 – 233Combined sources5
Turni235 – 240Combined sources6
Helixi245 – 248Combined sources4
Beta strandi251 – 256Combined sources6
Helixi257 – 259Combined sources3
Beta strandi266 – 270Combined sources5
Turni274 – 276Combined sources3
Helixi289 – 291Combined sources3
Helixi299 – 338Combined sources40
Turni340 – 342Combined sources3
Beta strandi343 – 345Combined sources3
Beta strandi358 – 363Combined sources6
Helixi370 – 376Combined sources7
Beta strandi378 – 381Combined sources4
Beta strandi383 – 385Combined sources3
Helixi387 – 389Combined sources3
Helixi398 – 402Combined sources5
Helixi407 – 410Combined sources4
Beta strandi413 – 417Combined sources5
Helixi424 – 442Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GZCX-ray2.10A/B8-445[»]
3GZDX-ray1.80A/B/C/D8-445[»]
ProteinModelPortaliQ96I15.
SMRiQ96I15.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96I15.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1549. Eukaryota.
COG1104. LUCA.
GeneTreeiENSGT00530000063513.
HOVERGENiHBG003708.
InParanoidiQ96I15.
KOiK01763.
PhylomeDBiQ96I15.
TreeFamiTF313550.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR016454. Cysteine_dSase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96I15-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAAVAPGRD APAPAASQPS GCGKHNSPER KVYMDYNATT PLEPEVIQAM
60 70 80 90 100
TKAMWEAWGN PSSPYSAGRK AKDIINAARE SLAKMIGGKP QDIIFTSGGT
110 120 130 140 150
ESNNLVIHSV VKHFHANQTS KGHTGGHHSP VKGAKPHFIT SSVEHDSIRL
160 170 180 190 200
PLEHLVEEQV AAVTFVPVSK VSGQAEVDDI LAAVRPTTRL VTIMLANNET
210 220 230 240 250
GIVMPVPEIS QRIKALNQER VAAGLPPILV HTDAAQALGK QRVDVEDLGV
260 270 280 290 300
DFLTIVGHKF YGPRIGALYI RGLGEFTPLY PMLFGGGQER NFRPGTENTP
310 320 330 340 350
MIAGLGKAAE LVTQNCEAYE AHMRDVRDYL EERLEAEFGQ KRIHLNSQFP
360 370 380 390 400
GTQRLPNTCN FSIRGPRLQG HVVLAQCRVL MASVGAACHS DHGDQPSPVL
410 420 430 440
LSYGVPFDVA RNALRLSVGR STTRAEVDLV VQDLKQAVAQ LEDQA
Length:445
Mass (Da):48,149
Last modified:July 24, 2013 - v4
Checksum:iD26F3FCFB8B165D2
GO
Isoform 2 (identifier: Q96I15-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-445: AAELVTQNCE...QAVAQLEDQA → VSPGELEEMS

Show »
Length:317
Mass (Da):33,957
Checksum:iA831471CE76E5B11
GO

Sequence cautioni

The sequence BAA91659 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → MPGSSGAGM in EAW71139 (Ref. 3) Curated1
Sequence conflicti42L → V in AAF36816 (PubMed:10692412).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03846452K → E.Corresponds to variant rs7597367dbSNPEnsembl.1
Natural variantiVAR_038465175A → T.1 PublicationCorresponds to variant rs3210400dbSNPEnsembl.1
Natural variantiVAR_038466276F → S.Corresponds to variant rs35637307dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030854308 – 445AAELV…LEDQA → VSPGELEEMS in isoform 2. 1 PublicationAdd BLAST138

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175767 mRNA. Translation: AAF36816.1.
AC016757 Genomic DNA. Translation: AAY24333.1.
AC016776 Genomic DNA. Translation: AAY24221.1.
CH471063 Genomic DNA. Translation: EAW71139.1.
BC000586 mRNA. Translation: AAH00586.2.
BC007891 mRNA. Translation: AAH07891.1.
AK001377 mRNA. Translation: BAA91659.1. Different initiation.
RefSeqiNP_057594.4. NM_016510.5.
UniGeneiHs.731909.

Genome annotation databases

EnsembliENST00000409736; ENSP00000387162; ENSG00000132330. [Q96I15-2]
GeneIDi51540.
KEGGihsa:51540.
UCSCiuc061uct.1. human. [Q96I15-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175767 mRNA. Translation: AAF36816.1.
AC016757 Genomic DNA. Translation: AAY24333.1.
AC016776 Genomic DNA. Translation: AAY24221.1.
CH471063 Genomic DNA. Translation: EAW71139.1.
BC000586 mRNA. Translation: AAH00586.2.
BC007891 mRNA. Translation: AAH07891.1.
AK001377 mRNA. Translation: BAA91659.1. Different initiation.
RefSeqiNP_057594.4. NM_016510.5.
UniGeneiHs.731909.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GZCX-ray2.10A/B8-445[»]
3GZDX-ray1.80A/B/C/D8-445[»]
ProteinModelPortaliQ96I15.
SMRiQ96I15.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119599. 32 interactors.
IntActiQ96I15. 10 interactors.
STRINGi9606.ENSP00000254663.

PTM databases

iPTMnetiQ96I15.
PhosphoSitePlusiQ96I15.

Polymorphism and mutation databases

BioMutaiSCLY.
DMDMi527504087.

Proteomic databases

EPDiQ96I15.
MaxQBiQ96I15.
PaxDbiQ96I15.
PeptideAtlasiQ96I15.
PRIDEiQ96I15.

Protocols and materials databases

DNASUi51540.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409736; ENSP00000387162; ENSG00000132330. [Q96I15-2]
GeneIDi51540.
KEGGihsa:51540.
UCSCiuc061uct.1. human. [Q96I15-1]

Organism-specific databases

CTDi51540.
DisGeNETi51540.
GeneCardsiSCLY.
H-InvDBHIX0114427.
HGNCiHGNC:18161. SCLY.
HPAiHPA020190.
HPA055760.
HPA062822.
MIMi611056. gene.
neXtProtiNX_Q96I15.
OpenTargetsiENSG00000132330.
PharmGKBiPA134979359.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1549. Eukaryota.
COG1104. LUCA.
GeneTreeiENSGT00530000063513.
HOVERGENiHBG003708.
InParanoidiQ96I15.
KOiK01763.
PhylomeDBiQ96I15.
TreeFamiTF313550.

Enzyme and pathway databases

BioCyciZFISH:HS05620-MONOMER.
ReactomeiR-HSA-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.

Miscellaneous databases

ChiTaRSiSCLY. human.
EvolutionaryTraceiQ96I15.
GenomeRNAii51540.
PROiQ96I15.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132330.
CleanExiHS_SCLY.
ExpressionAtlasiQ96I15. baseline and differential.
GenevisibleiQ96I15. HS.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR016454. Cysteine_dSase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSCLY_HUMAN
AccessioniPrimary (citable) accession number: Q96I15
Secondary accession number(s): B9A068
, J3KN06, Q53SN1, Q53SN8, Q7L670, Q9NVT7, Q9NZR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 24, 2013
Last modified: November 2, 2016
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.