Q96HR8 (NAF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
H/ACA ribonucleoprotein complex non-core subunit NAF1
Short name=hNAF1

Gene names

Name:

NAF1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4. Ref.5
Subunit structure	During assembly of the complex, component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs) which contains NOLA2/NHP2, NOLA3/NOP10, NAF1 and DKC1/NOLA4. Interacts directly with DKC1/NOLA4. Ref.5 Ref.6
Subcellular location	Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and the nucleus. Absent from the nucleolus By similarity. Ref.5 Ref.6 Ref.7
Sequence similarities	Belongs to the NAF1 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW snRNA pseudouridine synthesis Inferred from electronic annotation. Source: InterPro
Cellular component	cell junction Inferred from direct assay. Source: HPA centrosome Inferred from direct assay. Source: HPA nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell small nucleolar ribonucleoprotein complex Inferred from direct assay Ref.6Ref.7. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.6Ref.5. Source: UniProtKB snoRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 494

494

H/ACA ribonucleoprotein complex non-core subunit NAF1

PRO_0000315637

Regions

Compositional bias

110 – 148

39

Ser-rich

Compositional bias

417 – 481

65

Pro-rich

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.9

Modified residue

315

1

Phosphoserine Ref.4 Ref.8 Ref.9

Natural variations

Natural variant

43

1

P → S.
Corresponds to variant rs12331663 [ dbSNP | Ensembl ].

VAR_057795

Natural variant

162

1

I → V. Ref.3
Corresponds to variant rs4691896 [ dbSNP | Ensembl ].

VAR_063101

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

494

Helix Strand Turn

Details...

Beta strand

199 – 207

9

Beta strand

209 – 216

8

Beta strand

228 – 230

3

Beta strand

234 – 245

12

Beta strand

247 – 249

3

Beta strand

251 – 254

4

Helix

259 – 265

7

Beta strand

272 – 275

4

Sequences

	Sequence		Length	Mass (Da)
	Q96HR8 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 5A0C9B635A982A62	FASTA	494	53,717
	10 20 30 40 50 60 MEVVEAAAAQ LETLKFNGTD FGVGEGPAAP SPGSAPVPGT QPPLQSFEGS PDAGQTVEVK 70 80 90 100 110 120 PAGEQPLQPV LNAVAAGTPA PQPQPPAESP ACGDCVTSPG AAEPARAPDS LETSDSDSDS 130 140 150 160 170 180 DSETDSDSSS SSSSSSSSSS SSSSSCISLP PVLSDGDDDL QIEKENKNFP LKTKDELLLN 190 200 210 220 230 240 ELPSVEELTI ILPEDIELKP LGMVSSIIEQ LVIIESMTNL PPVNEETVIF KSDRQAAGKI 250 260 270 280 290 300 FEIFGPVAHP FYVLRFNSSD HIESKGIKIK ETMYFAPSMK DFTQYIFTEK LKQDKGSDAS 310 320 330 340 350 360 WKNDQEPPPE ALDFSDDEKE KEAKQRKKSQ IQGRKKLKSE FNEPGEDFTE VHQNWNAHSS 370 380 390 400 410 420 ASEHAKGYRN REFTRGFSRA RYPRSCHGRP PPQHFYNSEH MVSQETSGFP SQRQNNPIMP 430 440 450 460 470 480 QYPFPLPVFD MHNFPLRPPP PPPPPPVNMG WATPNMAAHP LLNLPYSLPP PPPPPPLPPP 490 PSSGDSNSHF GPYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-162. Tissue: Placenta.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells." Darzacq X., Kittur N., Roy S., Shav-Tal Y., Singer R.H., Meier U.T. J. Cell Biol. 173:207-218(2006) [PubMed: 16618814] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DKC1.
[6]	"hNaf1 is required for accumulation of human box H/ACA snoRNPs, scaRNPs, and telomerase." Hoareau-Aveilla C., Bonoli M., Caizergues-Ferrer M., Henry Y. RNA 12:832-840(2006) [PubMed: 16601202] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DKC1 AND NOLA3.
[7]	"Dynamic association and localization of human H/ACA RNP proteins." Kittur N., Darzacq X., Roy S., Singer R.H., Meier U.T. RNA 12:2057-2062(2006) [PubMed: 17135485] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[10]	"Solution structure of the NAF1 domain of hypothetical protein BC008207 [Homo sapiens]." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 181-276.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AC022272 Genomic DNA. No translation available.
CH471056 Genomic DNA. Translation: EAX04843.1.
CH471056 Genomic DNA. Translation: EAX04844.1.
BC008207 mRNA. Translation: AAH08207.1.

IPI

IPI00063219.

RefSeq

NP_001122403.1. NM_001128931.1.
NP_612395.2. NM_138386.2.

UniGene

Hs.129095.
Hs.672113.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EQN	NMR	-	A	181-276	[»]

ProteinModelPortal

Q96HR8.

SMR

Q96HR8. Positions 181-276.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96HR8. 1 interaction.

STRING

Q96HR8.

PTM databases

PhosphoSite

Q96HR8.

Polymorphism databases

DMDM

296439238.

Proteomic databases

PRIDE

Q96HR8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000274054; ENSP00000274054; ENSG00000145414.

GeneID

92345.

KEGG

hsa:92345.

UCSC

uc003iqj.1. human.

Organism-specific databases

CTD

92345.

GeneCards

GC04M164047.

H-InvDB

HIX0004605.

HGNC

HGNC:25126. NAF1.

HPA

HPA036242.

neXtProt

NX_Q96HR8.

PharmGKB

PA162396775.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG18125.

GeneTree

ENSGT00390000004697.

HOVERGEN

HBG108169.

InParanoid

Q96HR8.

OMA

MHNFSLP.

PhylomeDB

Q96HR8.

Gene expression databases

ArrayExpress

Q96HR8.

Bgee

Q96HR8.

CleanEx

HS_NAF1.

Genevestigator

Q96HR8.

Family and domain databases

InterPro

IPR007504. H/ACA_rnp_Gar1/Naf1.
IPR009000. Transl_elong_init/rib_B-barrel.
[Graphical view]

KO

K14763.

Pfam

PF04410. Gar1. 1 hit.
[Graphical view]

SUPFAM

SSF50447. Translat_factor. 1 hit.

ProtoNet

Search...

Entry information

Entry name

NAF1_HUMAN

Accession

Primary (citable) accession number: Q96HR8
Secondary accession number(s): D3DP28

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.