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Protein

Mediator of RNA polymerase II transcription subunit 30

Gene

MED30

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

GO - Molecular functioni

  • ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  • RNA polymerase II transcription cofactor activity Source: UniProtKB
  • signaling receptor activity Source: UniProtKB
  • thyroid hormone receptor binding Source: UniProtKB
  • transcription cofactor activity Source: UniProtKB
  • ubiquitin protein ligase activity Source: Ensembl
  • vitamin D receptor binding Source: UniProtKB

GO - Biological processi

  • androgen receptor signaling pathway Source: UniProtKB
  • intracellular steroid hormone receptor signaling pathway Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • stem cell population maintenance Source: Ensembl
  • transcription initiation from RNA polymerase II promoter Source: UniProtKB

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 30
Alternative name(s):
Mediator complex subunit 30
TRAP/Mediator complex component TRAP25
Thyroid hormone receptor-associated protein 6
Thyroid hormone receptor-associated protein complex 25 kDa component
Short name:
Trap25
Gene namesi
Name:MED30
Synonyms:THRAP6, TRAP25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000164758.7
HGNCiHGNC:23032 MED30
MIMi610237 gene
neXtProtiNX_Q96HR3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi90390
OpenTargetsiENSG00000164758
PharmGKBiPA162395656

Polymorphism and mutation databases

DMDMi74731968

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002394062 – 178Mediator of RNA polymerase II transcription subunit 30Add BLAST177

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ96HR3
MaxQBiQ96HR3
PaxDbiQ96HR3
PeptideAtlasiQ96HR3
PRIDEiQ96HR3

PTM databases

iPTMnetiQ96HR3
PhosphoSitePlusiQ96HR3

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000164758
CleanExiHS_MED30
GenevisibleiQ96HR3 HS

Organism-specific databases

HPAiHPA045767

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • thyroid hormone receptor binding Source: UniProtKB
  • vitamin D receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124707, 48 interactors
CORUMiQ96HR3
IntActiQ96HR3, 18 interactors
MINTiQ96HR3
STRINGi9606.ENSP00000297347

Structurei

3D structure databases

ProteinModelPortaliQ96HR3
SMRiQ96HR3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili70 – 94Sequence analysisAdd BLAST25
Coiled coili133 – 173Sequence analysisAdd BLAST41

Sequence similaritiesi

Belongs to the Mediator complex subunit 30 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IITZ Eukaryota
ENOG4110Q3G LUCA
GeneTreeiENSGT00390000010887
HOGENOMiHOG000006885
HOVERGENiHBG107279
InParanoidiQ96HR3
KOiK15143
OMAiNCAGLDP
OrthoDBiEOG091G0NPP
PhylomeDBiQ96HR3
TreeFamiTF324588

Family and domain databases

InterProiView protein in InterPro
IPR021019 Mediator_Med30_met
PANTHERiPTHR31705 PTHR31705, 1 hit
PfamiView protein in Pfam
PF11315 Med30, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96HR3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME
60 70 80 90 100
IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD NLRQLSVLFR KLRLVYDKCN
110 120 130 140 150
ENCGGMDPIP VEQLIPYVEE DGSKNDDRAG PPRFASEERR EIAEVNKKLK
160 170
QKNQQLKQIM DQLRNLIWDI NAMLAMRN
Length:178
Mass (Da):20,277
Last modified:December 1, 2001 - v1
Checksum:i1FD31212EDDF6238
GO
Isoform 2 (identifier: Q96HR3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-147: Missing.

Show »
Length:143
Mass (Da):16,279
Checksum:iEF5F536FFCDAC22F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_053904113 – 147Missing in isoform 2. 1 PublicationAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY083305 mRNA Translation: AAL89787.1
FM179284 mRNA Translation: CAQ76893.1
AC024329 Genomic DNA No translation available.
AC087361 Genomic DNA No translation available.
BC008226 mRNA Translation: AAH08226.1
CCDSiCCDS6323.1 [Q96HR3-1]
CCDS64959.1 [Q96HR3-2]
RefSeqiNP_001269915.1, NM_001282986.1 [Q96HR3-2]
NP_542382.1, NM_080651.3 [Q96HR3-1]
UniGeneiHs.492612

Genome annotation databases

EnsembliENST00000297347; ENSP00000297347; ENSG00000164758 [Q96HR3-1]
ENST00000522839; ENSP00000431051; ENSG00000164758 [Q96HR3-2]
GeneIDi90390
KEGGihsa:90390
UCSCiuc003yoj.4 human [Q96HR3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMED30_HUMAN
AccessioniPrimary (citable) accession number: Q96HR3
Secondary accession number(s): C6GKU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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