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Q96HR3

- MED30_HUMAN

UniProt

Q96HR3 - MED30_HUMAN

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Protein

Mediator of RNA polymerase II transcription subunit 30

Gene
MED30, THRAP6, TRAP25
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

GO - Molecular functioni

  1. ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  2. protein binding Source: UniProtKB
  3. receptor activity Source: UniProtKB
  4. RNA polymerase II transcription cofactor activity Source: UniProtKB
  5. thyroid hormone receptor binding Source: UniProtKB
  6. transcription cofactor activity Source: UniProtKB
  7. vitamin D receptor binding Source: UniProtKB

GO - Biological processi

  1. androgen receptor signaling pathway Source: UniProtKB
  2. gene expression Source: Reactome
  3. intracellular steroid hormone receptor signaling pathway Source: UniProtKB
  4. positive regulation of transcription, DNA-templated Source: UniProtKB
  5. regulation of transcription from RNA polymerase II promoter Source: GOC
  6. stem cell maintenance Source: Ensembl
  7. transcription initiation from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_12627. Generic Transcription Pathway.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 30
Alternative name(s):
Mediator complex subunit 30
TRAP/Mediator complex component TRAP25
Thyroid hormone receptor-associated protein 6
Thyroid hormone receptor-associated protein complex 25 kDa component
Short name:
Trap25
Gene namesi
Name:MED30
Synonyms:THRAP6, TRAP25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:23032. MED30.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. mediator complex Source: UniProtKB
  2. nucleoplasm Source: Reactome
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162395656.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 178177Mediator of RNA polymerase II transcription subunit 30PRO_0000239406Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine4 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ96HR3.
PaxDbiQ96HR3.
PRIDEiQ96HR3.

PTM databases

PhosphoSiteiQ96HR3.

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.1 Publication

Gene expression databases

ArrayExpressiQ96HR3.
BgeeiQ96HR3.
CleanExiHS_MED30.
GenevestigatoriQ96HR3.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.3 Publications

Protein-protein interaction databases

BioGridi124707. 36 interactions.
IntActiQ96HR3. 10 interactions.
MINTiMINT-3053560.
STRINGi9606.ENSP00000297347.

Structurei

3D structure databases

ProteinModelPortaliQ96HR3.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili70 – 9425 Reviewed predictionAdd
BLAST
Coiled coili133 – 17341 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG87821.
HOGENOMiHOG000006885.
HOVERGENiHBG107279.
InParanoidiQ96HR3.
KOiK15143.
OMAiGSKHDDR.
OrthoDBiEOG7C5MB0.
PhylomeDBiQ96HR3.
TreeFamiTF324588.

Family and domain databases

InterProiIPR021019. Mediator_Med30_met.
[Graphical view]
PfamiPF11315. Med30. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96HR3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME    50
IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD NLRQLSVLFR KLRLVYDKCN 100
ENCGGMDPIP VEQLIPYVEE DGSKNDDRAG PPRFASEERR EIAEVNKKLK 150
QKNQQLKQIM DQLRNLIWDI NAMLAMRN 178
Length:178
Mass (Da):20,277
Last modified:December 1, 2001 - v1
Checksum:i1FD31212EDDF6238
GO
Isoform 2 (identifier: Q96HR3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-147: Missing.

Show »
Length:143
Mass (Da):16,279
Checksum:iEF5F536FFCDAC22F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei113 – 14735Missing in isoform 2. VSP_053904Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY083305 mRNA. Translation: AAL89787.1.
FM179284 mRNA. Translation: CAQ76893.1.
AC024329 Genomic DNA. No translation available.
AC087361 Genomic DNA. No translation available.
BC008226 mRNA. Translation: AAH08226.1.
CCDSiCCDS6323.1. [Q96HR3-1]
CCDS64959.1. [Q96HR3-2]
RefSeqiNP_001269915.1. NM_001282986.1. [Q96HR3-2]
NP_542382.1. NM_080651.3. [Q96HR3-1]
UniGeneiHs.492612.

Genome annotation databases

EnsembliENST00000297347; ENSP00000297347; ENSG00000164758.
ENST00000522839; ENSP00000431051; ENSG00000164758.
GeneIDi90390.
KEGGihsa:90390.
UCSCiuc003yoj.3. human. [Q96HR3-1]

Polymorphism databases

DMDMi74731968.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY083305 mRNA. Translation: AAL89787.1 .
FM179284 mRNA. Translation: CAQ76893.1 .
AC024329 Genomic DNA. No translation available.
AC087361 Genomic DNA. No translation available.
BC008226 mRNA. Translation: AAH08226.1 .
CCDSi CCDS6323.1. [Q96HR3-1 ]
CCDS64959.1. [Q96HR3-2 ]
RefSeqi NP_001269915.1. NM_001282986.1. [Q96HR3-2 ]
NP_542382.1. NM_080651.3. [Q96HR3-1 ]
UniGenei Hs.492612.

3D structure databases

ProteinModelPortali Q96HR3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 124707. 36 interactions.
IntActi Q96HR3. 10 interactions.
MINTi MINT-3053560.
STRINGi 9606.ENSP00000297347.

PTM databases

PhosphoSitei Q96HR3.

Polymorphism databases

DMDMi 74731968.

Proteomic databases

MaxQBi Q96HR3.
PaxDbi Q96HR3.
PRIDEi Q96HR3.

Protocols and materials databases

DNASUi 90390.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000297347 ; ENSP00000297347 ; ENSG00000164758 .
ENST00000522839 ; ENSP00000431051 ; ENSG00000164758 .
GeneIDi 90390.
KEGGi hsa:90390.
UCSCi uc003yoj.3. human. [Q96HR3-1 ]

Organism-specific databases

CTDi 90390.
GeneCardsi GC08P118603.
HGNCi HGNC:23032. MED30.
MIMi 610237. gene.
neXtProti NX_Q96HR3.
PharmGKBi PA162395656.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG87821.
HOGENOMi HOG000006885.
HOVERGENi HBG107279.
InParanoidi Q96HR3.
KOi K15143.
OMAi GSKHDDR.
OrthoDBi EOG7C5MB0.
PhylomeDBi Q96HR3.
TreeFami TF324588.

Enzyme and pathway databases

Reactomei REACT_116145. PPARA activates gene expression.
REACT_12627. Generic Transcription Pathway.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

GeneWikii MED30.
GenomeRNAii 90390.
NextBioi 35483018.
PROi Q96HR3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q96HR3.
Bgeei Q96HR3.
CleanExi HS_MED30.
Genevestigatori Q96HR3.

Family and domain databases

InterProi IPR021019. Mediator_Med30_met.
[Graphical view ]
Pfami PF11315. Med30. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s."
    Baek H.J., Malik S., Qin J., Roeder R.G.
    Mol. Cell. Biol. 22:2842-2852(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, FUNCTION, TISSUE SPECIFICITY.
  2. "Identification of MED30 alternative mRNA in human progenitor cells."
    Rienzo M., Casamassimi A., Giovane A., Napoli C.
    Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
    Tissue: Bone marrow and Peripheral blood.
  3. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye.
  5. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
    Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
    J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED22.
  6. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  7. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED1; MED6; MED12; MED13; MED16; MED17; MED20; MED21 AND MED24, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  8. "Human Mediator enhances basal transcription by facilitating recruitment of transcription factor IIB during preinitiation complex assembly."
    Baek H.J., Kang Y.K., Roeder R.G.
    J. Biol. Chem. 281:15172-15181(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MED1 AND MED10.
  9. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMED30_HUMAN
AccessioniPrimary (citable) accession number: Q96HR3
Secondary accession number(s): C6GKU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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