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Q96HR3

- MED30_HUMAN

UniProt

Q96HR3 - MED30_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 30

Gene

MED30

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

    GO - Molecular functioni

    1. ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. receptor activity Source: UniProtKB
    4. RNA polymerase II transcription cofactor activity Source: UniProtKB
    5. thyroid hormone receptor binding Source: UniProtKB
    6. transcription cofactor activity Source: UniProtKB
    7. vitamin D receptor binding Source: UniProtKB

    GO - Biological processi

    1. androgen receptor signaling pathway Source: UniProtKB
    2. gene expression Source: Reactome
    3. intracellular steroid hormone receptor signaling pathway Source: UniProtKB
    4. positive regulation of transcription, DNA-templated Source: UniProtKB
    5. regulation of transcription from RNA polymerase II promoter Source: GOC
    6. stem cell maintenance Source: Ensembl
    7. transcription initiation from RNA polymerase II promoter Source: UniProtKB

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 30
    Alternative name(s):
    Mediator complex subunit 30
    TRAP/Mediator complex component TRAP25
    Thyroid hormone receptor-associated protein 6
    Thyroid hormone receptor-associated protein complex 25 kDa component
    Short name:
    Trap25
    Gene namesi
    Name:MED30
    Synonyms:THRAP6, TRAP25
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:23032. MED30.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: UniProtKB
    2. nucleoplasm Source: Reactome
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162395656.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed4 Publications
    Chaini2 – 178177Mediator of RNA polymerase II transcription subunit 30PRO_0000239406Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine4 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ96HR3.
    PaxDbiQ96HR3.
    PRIDEiQ96HR3.

    PTM databases

    PhosphoSiteiQ96HR3.

    Expressioni

    Tissue specificityi

    Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiQ96HR3.
    BgeeiQ96HR3.
    CleanExiHS_MED30.
    GenevestigatoriQ96HR3.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.3 Publications

    Protein-protein interaction databases

    BioGridi124707. 36 interactions.
    IntActiQ96HR3. 10 interactions.
    MINTiMINT-3053560.
    STRINGi9606.ENSP00000297347.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96HR3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili70 – 9425Sequence AnalysisAdd
    BLAST
    Coiled coili133 – 17341Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 30 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG87821.
    HOGENOMiHOG000006885.
    HOVERGENiHBG107279.
    InParanoidiQ96HR3.
    KOiK15143.
    OMAiGSKHDDR.
    OrthoDBiEOG7C5MB0.
    PhylomeDBiQ96HR3.
    TreeFamiTF324588.

    Family and domain databases

    InterProiIPR021019. Mediator_Med30_met.
    [Graphical view]
    PfamiPF11315. Med30. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96HR3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME    50
    IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD NLRQLSVLFR KLRLVYDKCN 100
    ENCGGMDPIP VEQLIPYVEE DGSKNDDRAG PPRFASEERR EIAEVNKKLK 150
    QKNQQLKQIM DQLRNLIWDI NAMLAMRN 178
    Length:178
    Mass (Da):20,277
    Last modified:December 1, 2001 - v1
    Checksum:i1FD31212EDDF6238
    GO
    Isoform 2 (identifier: Q96HR3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         113-147: Missing.

    Show »
    Length:143
    Mass (Da):16,279
    Checksum:iEF5F536FFCDAC22F
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei113 – 14735Missing in isoform 2. 1 PublicationVSP_053904Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY083305 mRNA. Translation: AAL89787.1.
    FM179284 mRNA. Translation: CAQ76893.1.
    AC024329 Genomic DNA. No translation available.
    AC087361 Genomic DNA. No translation available.
    BC008226 mRNA. Translation: AAH08226.1.
    CCDSiCCDS6323.1. [Q96HR3-1]
    CCDS64959.1. [Q96HR3-2]
    RefSeqiNP_001269915.1. NM_001282986.1. [Q96HR3-2]
    NP_542382.1. NM_080651.3. [Q96HR3-1]
    UniGeneiHs.492612.

    Genome annotation databases

    EnsembliENST00000297347; ENSP00000297347; ENSG00000164758. [Q96HR3-1]
    ENST00000522839; ENSP00000431051; ENSG00000164758. [Q96HR3-2]
    GeneIDi90390.
    KEGGihsa:90390.
    UCSCiuc003yoj.3. human. [Q96HR3-1]

    Polymorphism databases

    DMDMi74731968.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY083305 mRNA. Translation: AAL89787.1 .
    FM179284 mRNA. Translation: CAQ76893.1 .
    AC024329 Genomic DNA. No translation available.
    AC087361 Genomic DNA. No translation available.
    BC008226 mRNA. Translation: AAH08226.1 .
    CCDSi CCDS6323.1. [Q96HR3-1 ]
    CCDS64959.1. [Q96HR3-2 ]
    RefSeqi NP_001269915.1. NM_001282986.1. [Q96HR3-2 ]
    NP_542382.1. NM_080651.3. [Q96HR3-1 ]
    UniGenei Hs.492612.

    3D structure databases

    ProteinModelPortali Q96HR3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124707. 36 interactions.
    IntActi Q96HR3. 10 interactions.
    MINTi MINT-3053560.
    STRINGi 9606.ENSP00000297347.

    PTM databases

    PhosphoSitei Q96HR3.

    Polymorphism databases

    DMDMi 74731968.

    Proteomic databases

    MaxQBi Q96HR3.
    PaxDbi Q96HR3.
    PRIDEi Q96HR3.

    Protocols and materials databases

    DNASUi 90390.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000297347 ; ENSP00000297347 ; ENSG00000164758 . [Q96HR3-1 ]
    ENST00000522839 ; ENSP00000431051 ; ENSG00000164758 . [Q96HR3-2 ]
    GeneIDi 90390.
    KEGGi hsa:90390.
    UCSCi uc003yoj.3. human. [Q96HR3-1 ]

    Organism-specific databases

    CTDi 90390.
    GeneCardsi GC08P118603.
    HGNCi HGNC:23032. MED30.
    MIMi 610237. gene.
    neXtProti NX_Q96HR3.
    PharmGKBi PA162395656.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG87821.
    HOGENOMi HOG000006885.
    HOVERGENi HBG107279.
    InParanoidi Q96HR3.
    KOi K15143.
    OMAi GSKHDDR.
    OrthoDBi EOG7C5MB0.
    PhylomeDBi Q96HR3.
    TreeFami TF324588.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    GeneWikii MED30.
    GenomeRNAii 90390.
    NextBioi 35483018.
    PROi Q96HR3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96HR3.
    Bgeei Q96HR3.
    CleanExi HS_MED30.
    Genevestigatori Q96HR3.

    Family and domain databases

    InterProi IPR021019. Mediator_Med30_met.
    [Graphical view ]
    Pfami PF11315. Med30. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s."
      Baek H.J., Malik S., Qin J., Roeder R.G.
      Mol. Cell. Biol. 22:2842-2852(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, FUNCTION, TISSUE SPECIFICITY.
    2. "Identification of MED30 alternative mRNA in human progenitor cells."
      Rienzo M., Casamassimi A., Giovane A., Napoli C.
      Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
      Tissue: Bone marrow and Peripheral blood.
    3. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Eye.
    5. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
      Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
      J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED22.
    6. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    7. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED1; MED6; MED12; MED13; MED16; MED17; MED20; MED21 AND MED24, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    8. "Human Mediator enhances basal transcription by facilitating recruitment of transcription factor IIB during preinitiation complex assembly."
      Baek H.J., Kang Y.K., Roeder R.G.
      J. Biol. Chem. 281:15172-15181(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MED1 AND MED10.
    9. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMED30_HUMAN
    AccessioniPrimary (citable) accession number: Q96HR3
    Secondary accession number(s): C6GKU9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3