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Protein

Mediator of RNA polymerase II transcription subunit 30

Gene

MED30

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

GO - Molecular functioni

  • ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  • receptor activity Source: UniProtKB
  • RNA polymerase II transcription cofactor activity Source: UniProtKB
  • thyroid hormone receptor binding Source: UniProtKB
  • transcription cofactor activity Source: UniProtKB
  • vitamin D receptor binding Source: UniProtKB

GO - Biological processi

  • androgen receptor signaling pathway Source: UniProtKB
  • gene expression Source: Reactome
  • intracellular steroid hormone receptor signaling pathway Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: GOC
  • stem cell maintenance Source: Ensembl
  • transcription initiation from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_12627. Generic Transcription Pathway.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 30
Alternative name(s):
Mediator complex subunit 30
TRAP/Mediator complex component TRAP25
Thyroid hormone receptor-associated protein 6
Thyroid hormone receptor-associated protein complex 25 kDa component
Short name:
Trap25
Gene namesi
Name:MED30
Synonyms:THRAP6, TRAP25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:23032. MED30.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162395656.

Polymorphism and mutation databases

DMDMi74731968.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed4 Publications
Chaini2 – 178177Mediator of RNA polymerase II transcription subunit 30PRO_0000239406Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine4 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ96HR3.
PaxDbiQ96HR3.
PRIDEiQ96HR3.

PTM databases

PhosphoSiteiQ96HR3.

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ96HR3.
CleanExiHS_MED30.
GenevisibleiQ96HR3. HS.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DOCK8Q8NF50-23EBI-394659,EBI-10174653
KRTAP10-9P604113EBI-394659,EBI-10172052

Protein-protein interaction databases

BioGridi124707. 40 interactions.
IntActiQ96HR3. 12 interactions.
MINTiMINT-3053560.
STRINGi9606.ENSP00000297347.

Structurei

3D structure databases

ProteinModelPortaliQ96HR3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili70 – 9425Sequence AnalysisAdd
BLAST
Coiled coili133 – 17341Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 30 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG87821.
GeneTreeiENSGT00390000010887.
HOGENOMiHOG000006885.
HOVERGENiHBG107279.
InParanoidiQ96HR3.
KOiK15143.
OMAiDIVLRTM.
OrthoDBiEOG7C5MB0.
PhylomeDBiQ96HR3.
TreeFamiTF324588.

Family and domain databases

InterProiIPR021019. Mediator_Med30_met.
[Graphical view]
PfamiPF11315. Med30. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96HR3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME
60 70 80 90 100
IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD NLRQLSVLFR KLRLVYDKCN
110 120 130 140 150
ENCGGMDPIP VEQLIPYVEE DGSKNDDRAG PPRFASEERR EIAEVNKKLK
160 170
QKNQQLKQIM DQLRNLIWDI NAMLAMRN
Length:178
Mass (Da):20,277
Last modified:December 1, 2001 - v1
Checksum:i1FD31212EDDF6238
GO
Isoform 2 (identifier: Q96HR3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-147: Missing.

Show »
Length:143
Mass (Da):16,279
Checksum:iEF5F536FFCDAC22F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei113 – 14735Missing in isoform 2. 1 PublicationVSP_053904Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY083305 mRNA. Translation: AAL89787.1.
FM179284 mRNA. Translation: CAQ76893.1.
AC024329 Genomic DNA. No translation available.
AC087361 Genomic DNA. No translation available.
BC008226 mRNA. Translation: AAH08226.1.
CCDSiCCDS6323.1. [Q96HR3-1]
CCDS64959.1. [Q96HR3-2]
RefSeqiNP_001269915.1. NM_001282986.1. [Q96HR3-2]
NP_542382.1. NM_080651.3. [Q96HR3-1]
UniGeneiHs.492612.

Genome annotation databases

EnsembliENST00000297347; ENSP00000297347; ENSG00000164758.
ENST00000522839; ENSP00000431051; ENSG00000164758. [Q96HR3-2]
GeneIDi90390.
KEGGihsa:90390.
UCSCiuc003yoj.3. human. [Q96HR3-1]
uc011lib.2. human.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY083305 mRNA. Translation: AAL89787.1.
FM179284 mRNA. Translation: CAQ76893.1.
AC024329 Genomic DNA. No translation available.
AC087361 Genomic DNA. No translation available.
BC008226 mRNA. Translation: AAH08226.1.
CCDSiCCDS6323.1. [Q96HR3-1]
CCDS64959.1. [Q96HR3-2]
RefSeqiNP_001269915.1. NM_001282986.1. [Q96HR3-2]
NP_542382.1. NM_080651.3. [Q96HR3-1]
UniGeneiHs.492612.

3D structure databases

ProteinModelPortaliQ96HR3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124707. 40 interactions.
IntActiQ96HR3. 12 interactions.
MINTiMINT-3053560.
STRINGi9606.ENSP00000297347.

PTM databases

PhosphoSiteiQ96HR3.

Polymorphism and mutation databases

DMDMi74731968.

Proteomic databases

MaxQBiQ96HR3.
PaxDbiQ96HR3.
PRIDEiQ96HR3.

Protocols and materials databases

DNASUi90390.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000297347; ENSP00000297347; ENSG00000164758.
ENST00000522839; ENSP00000431051; ENSG00000164758. [Q96HR3-2]
GeneIDi90390.
KEGGihsa:90390.
UCSCiuc003yoj.3. human. [Q96HR3-1]
uc011lib.2. human.

Organism-specific databases

CTDi90390.
GeneCardsiGC08P118603.
HGNCiHGNC:23032. MED30.
MIMi610237. gene.
neXtProtiNX_Q96HR3.
PharmGKBiPA162395656.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG87821.
GeneTreeiENSGT00390000010887.
HOGENOMiHOG000006885.
HOVERGENiHBG107279.
InParanoidiQ96HR3.
KOiK15143.
OMAiDIVLRTM.
OrthoDBiEOG7C5MB0.
PhylomeDBiQ96HR3.
TreeFamiTF324588.

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_12627. Generic Transcription Pathway.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiMED30. human.
GeneWikiiMED30.
GenomeRNAii90390.
NextBioi35483018.
PROiQ96HR3.
SOURCEiSearch...

Gene expression databases

BgeeiQ96HR3.
CleanExiHS_MED30.
GenevisibleiQ96HR3. HS.

Family and domain databases

InterProiIPR021019. Mediator_Med30_met.
[Graphical view]
PfamiPF11315. Med30. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s."
    Baek H.J., Malik S., Qin J., Roeder R.G.
    Mol. Cell. Biol. 22:2842-2852(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, FUNCTION, TISSUE SPECIFICITY.
  2. "Identification of MED30 alternative mRNA in human progenitor cells."
    Rienzo M., Casamassimi A., Giovane A., Napoli C.
    Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
    Tissue: Bone marrow and Peripheral blood.
  3. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye.
  5. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
    Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
    J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED22.
  6. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  7. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED1; MED6; MED12; MED13; MED16; MED17; MED20; MED21 AND MED24, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  8. "Human Mediator enhances basal transcription by facilitating recruitment of transcription factor IIB during preinitiation complex assembly."
    Baek H.J., Kang Y.K., Roeder R.G.
    J. Biol. Chem. 281:15172-15181(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MED1 AND MED10.
  9. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMED30_HUMAN
AccessioniPrimary (citable) accession number: Q96HR3
Secondary accession number(s): C6GKU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: December 1, 2001
Last modified: July 22, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.