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Protein

Adenosylhomocysteinase 3

Gene

AHCYL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May regulate the electrogenic sodium/bicarbonate cotransporter SLC4A4 activity and Mg2+-sensitivity. On the contrary of its homolog AHCYL1, does not regulate ITPR1 sensitivity to inositol 1,4,5-trisphosphate (PubMed:19220705).By similarity1 Publication

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.

Cofactori

NAD+Note: Binds 1 NAD+ per subunit.

Pathwayi: L-homocysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylhomocysteinase (AHCYL1), Adenosylhomocysteinase (AHCYL2), Adenosylhomocysteinase (AHCYL1), Adenosylhomocysteinase (AHCY), Adenosylhomocysteinase, Adenosylhomocysteinase (AHCY), Adenosylhomocysteinase 2 (AHCYL1), Adenosylhomocysteinase (FGFR2-AHCYL1), Adenosylhomocysteinase 3 (AHCYL2)
This subpathway is part of the pathway L-homocysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei236SubstrateBy similarity1
Binding sitei310SubstrateBy similarity1
Binding sitei335SubstrateBy similarity1
Binding sitei365SubstrateBy similarity1
Binding sitei369SubstrateBy similarity1
Binding sitei370NADBy similarity1
Binding sitei422NAD1 Publication1
Binding sitei457NAD1 Publication1
Binding sitei525NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi336 – 338NADBy similarity3
Nucleotide bindingi401 – 406NAD1 Publication6
Nucleotide bindingi478 – 479NAD1 Publication2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciZFISH:HS08293-MONOMER.
ReactomeiR-HSA-425381. Bicarbonate transporters.
UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylhomocysteinase 3 (EC:3.3.1.1)
Short name:
AdoHcyase 3
Alternative name(s):
IP(3)Rs binding protein released with IP(3) 2
Short name:
IRBIT2
Long-IRBIT1 Publication
S-adenosyl-L-homocysteine hydrolase 3
S-adenosylhomocysteine hydrolase-like protein 2
Gene namesi
Name:AHCYL2
Synonyms:KIAA0828
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:22204. AHCYL2.

Subcellular locationi

  • Cytoplasm By similarity
  • Microsome By similarity

  • Note: Associates with membranes when phosphorylated, probably through interaction with ITPR1.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome

Pathology & Biotechi

Organism-specific databases

DisGeNETi23382.
OpenTargetsiENSG00000158467.
PharmGKBiPA162376046.

Polymorphism and mutation databases

BioMutaiAHCYL2.
DMDMi21759427.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001169092 – 611Adenosylhomocysteinase 3Add BLAST610

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei107PhosphoserineCombined sources1
Modified residuei121N6-acetyllysineBy similarity1
Modified residuei149Phosphoserine1 Publication1
Modified residuei152Phosphoserine1 Publication1
Modified residuei155Phosphoserine1 Publication1
Modified residuei158Phosphoserine1 Publication1
Modified residuei165PhosphoserineBy similarity1
Modified residuei472PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated during neuronal diffrentiation at the LISN domain.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96HN2.
MaxQBiQ96HN2.
PaxDbiQ96HN2.
PeptideAtlasiQ96HN2.
PRIDEiQ96HN2.

PTM databases

iPTMnetiQ96HN2.
PhosphoSitePlusiQ96HN2.

Expressioni

Gene expression databases

BgeeiENSG00000158467.
CleanExiHS_AHCYL2.
ExpressionAtlasiQ96HN2. baseline and differential.
GenevisibleiQ96HN2. HS.

Organism-specific databases

HPAiHPA053128.

Interactioni

Subunit structurei

Homotetramer (Probable). Forms heteromultimers with AHCYL1 (via the C-terminal region) (PubMed:19220705). Interacts with ITPR1; with lower affinity than AHCYL1 and maybe via ITPR1 (PubMed:19220705). Interacts with SLC4A4 (By similarity).By similarity1 Publication1 Publication

Protein-protein interaction databases

BioGridi116958. 65 interactors.
IntActiQ96HN2. 8 interactors.
MINTiMINT-5005542.
STRINGi9606.ENSP00000315931.

Structurei

Secondary structure

1611
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi191 – 193Combined sources3
Helixi194 – 206Combined sources13
Helixi209 – 218Combined sources10
Turni223 – 226Combined sources4
Beta strandi228 – 233Combined sources6
Helixi237 – 248Combined sources12
Beta strandi252 – 261Combined sources10
Helixi265 – 274Combined sources10
Helixi286 – 297Combined sources12
Beta strandi305 – 312Combined sources8
Helixi313 – 321Combined sources9
Helixi323 – 327Combined sources5
Beta strandi331 – 334Combined sources4
Helixi337 – 343Combined sources7
Beta strandi356 – 358Combined sources3
Helixi363 – 369Combined sources7
Helixi371 – 386Combined sources16
Beta strandi394 – 398Combined sources5
Helixi402 – 413Combined sources12
Beta strandi417 – 421Combined sources5
Helixi425 – 433Combined sources9
Helixi441 – 444Combined sources4
Turni445 – 447Combined sources3
Beta strandi449 – 453Combined sources5
Helixi463 – 468Combined sources6
Beta strandi473 – 477Combined sources5
Turni481 – 484Combined sources4
Helixi487 – 490Combined sources4
Beta strandi496 – 501Combined sources6
Beta strandi504 – 508Combined sources5
Beta strandi514 – 518Combined sources5
Helixi519 – 521Combined sources3
Helixi524 – 528Combined sources5
Helixi533 – 552Combined sources20
Turni555 – 557Combined sources3
Beta strandi560 – 564Combined sources5
Helixi567 – 577Combined sources11
Helixi578 – 581Combined sources4
Helixi590 – 596Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GVPX-ray2.25A/B/C/D175-607[»]
ProteinModelPortaliQ96HN2.
SMRiQ96HN2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96HN2.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 109LISN domain, inhibits interaction with ITPR11 PublicationAdd BLAST108

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.Curated

Phylogenomic databases

eggNOGiKOG1370. Eukaryota.
COG0499. LUCA.
GeneTreeiENSGT00390000003626.
HOGENOMiHOG000227986.
HOVERGENiHBG005041.
InParanoidiQ96HN2.
KOiK01251.
OMAiFKMIKGI.
OrthoDBiEOG091G06EB.
PhylomeDBiQ96HN2.
TreeFamiTF300415.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96HN2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVQVVSAAA AAKVPEVELK DLSPSEAESQ LGLSTAAVGA MAPPAGGGDP
60 70 80 90 100
EAPAPAAERP PVPGPGSGPA AALSPAAGKV PQASAMKRSD PHHQHQRHRD
110 120 130 140 150
GGEALVSPDG TVTEAPRTVK KQIQFADQKQ EFNKRPTKIG RRSLSRSISQ
160 170 180 190 200
SSTDSYSSAA SYTDSSDDET SPRDKQQKNS KGSSDFCVKN IKQAEFGRRE
210 220 230 240 250
IEIAEQEMPA LMALRKRAQG EKPLAGAKIV GCTHITAQTA VLMETLGALG
260 270 280 290 300
AQCRWAACNI YSTLNEVAAA LAESGFPVFA WKGESEDDFW WCIDRCVNVE
310 320 330 340 350
GWQPNMILDD GGDLTHWIYK KYPNMFKKIK GIVEESVTGV HRLYQLSKAG
360 370 380 390 400
KLCVPAMNVN DSVTKQKFDN LYCCRESILD GLKRTTDMMF GGKQVVVCGY
410 420 430 440 450
GEVGKGCCAA LKAMGSIVYV TEIDPICALQ ACMDGFRLVK LNEVIRQVDI
460 470 480 490 500
VITCTGNKNV VTREHLDRMK NSCIVCNMGH SNTEIDVASL RTPELTWERV
510 520 530 540 550
RSQVDHVIWP DGKRIVLLAE GRLLNLSCST VPTFVLSITA TTQALALIEL
560 570 580 590 600
YNAPEGRYKQ DVYLLPKKMD EYVASLHLPT FDAHLTELTD EQAKYLGLNK
610
NGPFKPNYYR Y
Length:611
Mass (Da):66,721
Last modified:December 1, 2001 - v1
Checksum:iA76B1275FC0D891D
GO
Isoform 2 (identifier: Q96HN2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     122-122: Missing.

Note: No experimental confirmation available.
Show »
Length:610
Mass (Da):66,593
Checksum:iD54ED0745D3A286B
GO
Isoform 3 (identifier: Q96HN2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MSVQVVSAAAAAKVPEVE → MLGSKKKYIVNGNSGIKA
     19-121: Missing.

Note: No experimental confirmation available.
Show »
Length:508
Mass (Da):56,697
Checksum:i1198694128CB2301
GO
Isoform 4 (identifier: Q96HN2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MSVQVVSAAAAAKVPEVEL → MEKWDGNEGTSAFHMPEWM
     20-122: Missing.

Note: No experimental confirmation available.
Show »
Length:508
Mass (Da):56,944
Checksum:i197CA80AF2940465
GO

Sequence cautioni

The sequence BAA74851 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0462781 – 19MSVQV…PEVEL → MEKWDGNEGTSAFHMPEWM in isoform 4. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_0431851 – 18MSVQV…VPEVE → MLGSKKKYIVNGNSGIKA in isoform 3. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_04318619 – 121Missing in isoform 3. 1 PublicationAdd BLAST103
Alternative sequenceiVSP_04627920 – 122Missing in isoform 4. 1 PublicationAdd BLAST103
Alternative sequenceiVSP_040095122Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020635 mRNA. Translation: BAA74851.1. Different initiation.
AK025372 mRNA. No translation available.
AK295851 mRNA. Translation: BAG58657.1.
AK316073 mRNA. Translation: BAH14444.1.
AC009244 Genomic DNA. No translation available.
AC011005 Genomic DNA. No translation available.
AC083866 Genomic DNA. No translation available.
AC093149 Genomic DNA. No translation available.
BC008349 mRNA. Translation: AAH08349.1.
BC024325 mRNA. Translation: AAH24325.1.
CCDSiCCDS47706.1. [Q96HN2-2]
CCDS47707.2. [Q96HN2-3]
CCDS47708.1. [Q96HN2-4]
CCDS5812.1. [Q96HN2-1]
RefSeqiNP_001124192.1. NM_001130720.2. [Q96HN2-2]
NP_001124194.2. NM_001130722.2. [Q96HN2-3]
NP_001124195.1. NM_001130723.2. [Q96HN2-4]
NP_056143.1. NM_015328.3. [Q96HN2-1]
UniGeneiHs.600789.

Genome annotation databases

EnsembliENST00000325006; ENSP00000315931; ENSG00000158467. [Q96HN2-1]
ENST00000446544; ENSP00000413639; ENSG00000158467. [Q96HN2-2]
ENST00000474594; ENSP00000420459; ENSG00000158467. [Q96HN2-4]
ENST00000490911; ENSP00000420801; ENSG00000158467. [Q96HN2-3]
GeneIDi23382.
KEGGihsa:23382.
UCSCiuc003vot.4. human. [Q96HN2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020635 mRNA. Translation: BAA74851.1. Different initiation.
AK025372 mRNA. No translation available.
AK295851 mRNA. Translation: BAG58657.1.
AK316073 mRNA. Translation: BAH14444.1.
AC009244 Genomic DNA. No translation available.
AC011005 Genomic DNA. No translation available.
AC083866 Genomic DNA. No translation available.
AC093149 Genomic DNA. No translation available.
BC008349 mRNA. Translation: AAH08349.1.
BC024325 mRNA. Translation: AAH24325.1.
CCDSiCCDS47706.1. [Q96HN2-2]
CCDS47707.2. [Q96HN2-3]
CCDS47708.1. [Q96HN2-4]
CCDS5812.1. [Q96HN2-1]
RefSeqiNP_001124192.1. NM_001130720.2. [Q96HN2-2]
NP_001124194.2. NM_001130722.2. [Q96HN2-3]
NP_001124195.1. NM_001130723.2. [Q96HN2-4]
NP_056143.1. NM_015328.3. [Q96HN2-1]
UniGeneiHs.600789.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GVPX-ray2.25A/B/C/D175-607[»]
ProteinModelPortaliQ96HN2.
SMRiQ96HN2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116958. 65 interactors.
IntActiQ96HN2. 8 interactors.
MINTiMINT-5005542.
STRINGi9606.ENSP00000315931.

PTM databases

iPTMnetiQ96HN2.
PhosphoSitePlusiQ96HN2.

Polymorphism and mutation databases

BioMutaiAHCYL2.
DMDMi21759427.

Proteomic databases

EPDiQ96HN2.
MaxQBiQ96HN2.
PaxDbiQ96HN2.
PeptideAtlasiQ96HN2.
PRIDEiQ96HN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325006; ENSP00000315931; ENSG00000158467. [Q96HN2-1]
ENST00000446544; ENSP00000413639; ENSG00000158467. [Q96HN2-2]
ENST00000474594; ENSP00000420459; ENSG00000158467. [Q96HN2-4]
ENST00000490911; ENSP00000420801; ENSG00000158467. [Q96HN2-3]
GeneIDi23382.
KEGGihsa:23382.
UCSCiuc003vot.4. human. [Q96HN2-1]

Organism-specific databases

CTDi23382.
DisGeNETi23382.
GeneCardsiAHCYL2.
HGNCiHGNC:22204. AHCYL2.
HPAiHPA053128.
MIMi616520. gene.
neXtProtiNX_Q96HN2.
OpenTargetsiENSG00000158467.
PharmGKBiPA162376046.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1370. Eukaryota.
COG0499. LUCA.
GeneTreeiENSGT00390000003626.
HOGENOMiHOG000227986.
HOVERGENiHBG005041.
InParanoidiQ96HN2.
KOiK01251.
OMAiFKMIKGI.
OrthoDBiEOG091G06EB.
PhylomeDBiQ96HN2.
TreeFamiTF300415.

Enzyme and pathway databases

UniPathwayiUPA00314; UER00076.
BioCyciZFISH:HS08293-MONOMER.
ReactomeiR-HSA-425381. Bicarbonate transporters.

Miscellaneous databases

ChiTaRSiAHCYL2. human.
EvolutionaryTraceiQ96HN2.
GenomeRNAii23382.
PROiQ96HN2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000158467.
CleanExiHS_AHCYL2.
ExpressionAtlasiQ96HN2. baseline and differential.
GenevisibleiQ96HN2. HS.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAHH3_HUMAN
AccessioniPrimary (citable) accession number: Q96HN2
Secondary accession number(s): B4DIZ5, D9N155, O94917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.