Q96HL8 (SH3Y1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SH3 domain-containing YSC84-like protein 1

Gene names

Name:

SH3YL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with SH3D19 By similarity.
Sequence similarities	Belongs to the SH3YL1 family. Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	SH3 domain
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidylinositol biosynthetic process Inferred from electronic annotation. Source: Compara regulation of ruffle assembly Inferred from electronic annotation. Source: Compara
Cellular_component	ruffle membrane Inferred from direct assay PubMed 21624956. Source: MGI
Molecular_function	phosphatase binding Inferred from direct assay PubMed 21624956. Source: MGI phosphatidylinositol binding Inferred from direct assay PubMed 21624956. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96HL8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96HL8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     262-280: Missing.

Isoform 3 (identifier: Q96HL8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-96: Missing.
     97-97: E → M

Isoform 4 (identifier: Q96HL8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-96: Missing.
     97-97: E → M
     262-280: Missing.

Isoform 5 (identifier: Q96HL8-5)

The sequence of this isoform differs from the canonical sequence as follows:
     98-342: VSDLVIILNY...IFPANYVTMN → THLQLHIPSEHCPGCLP

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 342

342

SH3 domain-containing YSC84-like protein 1

PRO_0000341560

Regions

Domain

283 – 342

60

SH3

Natural variations

Alternative sequence

1 – 96

96

Missing in isoform 3 and isoform 4.

VSP_034332

Alternative sequence

97

1

E → M in isoform 3 and isoform 4.

VSP_034333

Alternative sequence

98 – 342

245

VSDLV…YVTMN → THLQLHIPSEHCPGCLP in isoform 5.

VSP_034334

Alternative sequence

262 – 280

19

Missing in isoform 2 and isoform 4.

VSP_034335

Experimental info

Sequence conflict

4

1

P → H in AAH34974. Ref.5

Sequence conflict

4

1

P → H in AAH30778. Ref.5

Sequence conflict

104

1

I → V in BAF85001. Ref.1

Sequence conflict

141

1

V → M in BAF85001. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

342

Helix Strand Turn

Details...

Beta strand

286 – 289

4

Beta strand

309 – 314

6

Beta strand

319 – 327

9

Beta strand

330 – 335

6

Helix

336 – 338

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A12F0276A870B863	FASTA	342	37,148
	10 20 30 40 50 60 MNNPIPSNLK SEAKKAAKIL REFTEITSRN GPDKIIPAHV IAKAKGLAIL SVIKAGFLVT 70 80 90 100 110 120 ARGGSGIVVA RLPDGKWSAP SAIGIAGLGG GFEIGIEVSD LVIILNYDRA VEAFAKGGNL 130 140 150 160 170 180 TLGGNLTVAV GPLGRNLEGN VALRSSAAVF TYCKSRGLFA GVSLEGSCLI ERKETNRKFY 190 200 210 220 230 240 CQDIRAYDIL FGDTPRPAQA EDLYEILDSF TEKYENEGQR INARKAAREQ RKSSAKELPP 250 260 270 280 290 300 KPLSRPQQSS APVQLNSGSQ SNRNEYKLYP GLSSYHERVG NLNQPIEVTA LYSFEGQQPG 310 320 330 340 DLNFQAGDRI TVISKTDSHF DWWEGKLRGQ TGIFPANYVT MN
	Isoform 2 [UniParc]. Checksum: 0978372B41715F8F	FASTA	323	34,884
	10 20 30 40 50 60 MNNPIPSNLK SEAKKAAKIL REFTEITSRN GPDKIIPAHV IAKAKGLAIL SVIKAGFLVT 70 80 90 100 110 120 ARGGSGIVVA RLPDGKWSAP SAIGIAGLGG GFEIGIEVSD LVIILNYDRA VEAFAKGGNL 130 140 150 160 170 180 TLGGNLTVAV GPLGRNLEGN VALRSSAAVF TYCKSRGLFA GVSLEGSCLI ERKETNRKFY 190 200 210 220 230 240 CQDIRAYDIL FGDTPRPAQA EDLYEILDSF TEKYENEGQR INARKAAREQ RKSSAKELPP 250 260 270 280 290 300 KPLSRPQQSS APVQLNSGSQ SNLNQPIEVT ALYSFEGQQP GDLNFQAGDR ITVISKTDSH 310 320 FDWWEGKLRG QTGIFPANYV TMN
	Isoform 3 [UniParc]. Checksum: CCB998B271C72E7E	FASTA	246	27,353
	10 20 30 40 50 60 MVSDLVIILN YDRAVEAFAK GGNLTLGGNL TVAVGPLGRN LEGNVALRSS AAVFTYCKSR 70 80 90 100 110 120 GLFAGVSLEG SCLIERKETN RKFYCQDIRA YDILFGDTPR PAQAEDLYEI LDSFTEKYEN 130 140 150 160 170 180 EGQRINARKA AREQRKSSAK ELPPKPLSRP QQSSAPVQLN SGSQSNRNEY KLYPGLSSYH 190 200 210 220 230 240 ERVGNLNQPI EVTALYSFEG QQPGDLNFQA GDRITVISKT DSHFDWWEGK LRGQTGIFPA NYVTMN
	Isoform 4 [UniParc]. Checksum: 42B6CACE64E66697	FASTA	227	25,088
	10 20 30 40 50 60 MVSDLVIILN YDRAVEAFAK GGNLTLGGNL TVAVGPLGRN LEGNVALRSS AAVFTYCKSR 70 80 90 100 110 120 GLFAGVSLEG SCLIERKETN RKFYCQDIRA YDILFGDTPR PAQAEDLYEI LDSFTEKYEN 130 140 150 160 170 180 EGQRINARKA AREQRKSSAK ELPPKPLSRP QQSSAPVQLN SGSQSNLNQP IEVTALYSFE 190 200 210 220 GQQPGDLNFQ AGDRITVISK TDSHFDWWEG KLRGQTGIFP ANYVTMN
	Isoform 5 [UniParc]. Checksum: 28BF2A1DA409BE24	FASTA	114	11,809
	10 20 30 40 50 60 MNNPIPSNLK SEAKKAAKIL REFTEITSRN GPDKIIPAHV IAKAKGLAIL SVIKAGFLVT 70 80 90 100 110 ARGGSGIVVA RLPDGKWSAP SAIGIAGLGG GFEIGIETHL QLHIPSEHCP GCLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4). Tissue: Carcinoma and Testis.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Uterus.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 5). Tissue: Testis and Urinary bladder.
[6]	"Solution structure of the SH3 domain of hypothetical protein SH3YL1." RIKEN structural genomics initiative (RSGI) Submitted (FEB-2009) to the PDB data bank Cited for: STRUCTURE BY NMR OF 273-342.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK026507 mRNA. Translation: BAB15493.1.
AK292312 mRNA. Translation: BAF85001.1.
CR457361 mRNA. Translation: CAG33642.1.
AL050373 mRNA. Translation: CAB43679.2.
AC079779 Genomic DNA. Translation: AAY14957.1.
BC008374 mRNA. Translation: AAH08374.1.
BC008375 mRNA. Translation: AAH08375.1.
BC030778 mRNA. Translation: AAH30778.1.
BC034974 mRNA. Translation: AAH34974.1.

IPI

IPI00182774.
IPI00395699.
IPI00872745.
IPI00892785.
IPI00893448.

RefSeq

NP_001153069.1. NM_001159597.1.
NP_056492.2. NM_015677.2.

UniGene

Hs.730730.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D8H	NMR	-	A	273-342	[»]

ProteinModelPortal

Q96HL8.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96HL8. 2 interactions.

MINT

MINT-1397835.

STRING

9606.ENSP00000404143.

PTM databases

PhosphoSite

Q96HL8.

Polymorphism databases

DMDM

74751912.

Proteomic databases

PaxDb

Q96HL8.

PRIDE

Q96HL8.

Protocols and materials databases

DNASU

26751.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000356150; ENSP00000348471; ENSG00000035115.
ENST00000402632; ENSP00000384910; ENSG00000035115.
ENST00000403657; ENSP00000385668; ENSG00000035115.
ENST00000403658; ENSP00000383928; ENSG00000035115.
ENST00000403712; ENSP00000384276; ENSG00000035115.
ENST00000405430; ENSP00000384269; ENSG00000035115.
ENST00000415006; ENSP00000404143; ENSG00000035115.

GeneID

26751.

KEGG

hsa:26751.

UCSC

uc002qvv.3. human.
uc002qvx.3. human.
uc002qvy.3. human.
uc010ewe.3. human.

Organism-specific databases

CTD

26751.

GeneCards

GC02M000208.

H-InvDB

HIX0001777.

HGNC

HGNC:29546. SH3YL1.

HPA

HPA030926.
HPA030927.

neXtProt

NX_Q96HL8.

PharmGKB

PA128394643.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG2930.

HOGENOM

HOG000215564.

HOVERGEN

HBG108487.

InParanoid

Q96HL8.

OMA

ELSSYHE.

Gene expression databases

ArrayExpress

Q96HL8.

Bgee

Q96HL8.

CleanEx

HS_SH3YL1.

Genevestigator

Q96HL8.

Family and domain databases

InterPro

IPR001452. SH3_domain.
IPR007461. Ysc84_actin-binding.
[Graphical view]

Pfam

PF04366. DUF500. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

PRINTS

PR00452. SH3DOMAIN.

SMART

SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q96HL8.

GenomeRNAi

26751.

NextBio

49119.

Entry information

Entry name

SH3Y1_HUMAN

Accession

Primary (citable) accession number: Q96HL8
Secondary accession number(s): A8K8E7 , B7WNJ4, B7WPL6, Q8NEL2, Q9H5X4, Q9Y3V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.