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Q96HJ5

- MS4A3_HUMAN

UniProt

Q96HJ5 - MS4A3_HUMAN

Protein

Membrane-spanning 4-domains subfamily A member 3

Gene

MS4A3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Hematopoietic modulator for the G1-S cell cycle transition. Modulates the level of phosphorylation of cyclin-dependent kinase 2 (CDK2) through its direct binding to cyclin-dependent kinase inhibitor 3 (CDKN3/KAP).1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    Keywords - Molecular functioni

    Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Membrane-spanning 4-domains subfamily A member 3
    Alternative name(s):
    CD20 antigen-like protein
    Hematopoietic-specific transmembrane protein 4
    Short name:
    HTm4
    Gene namesi
    Name:MS4A3
    Synonyms:CD20L, HTM4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:7317. MS4A3.

    Subcellular locationi

    Endomembrane system By similarity; Multi-pass membrane protein By similarity. Cytoplasmperinuclear region By similarity
    Note: Located in the perinuclear area.

    GO - Cellular componenti

    1. endomembrane system Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31112.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 214214Membrane-spanning 4-domains subfamily A member 3PRO_0000158632Add
    BLAST

    Proteomic databases

    PaxDbiQ96HJ5.
    PRIDEiQ96HJ5.

    Expressioni

    Tissue specificityi

    Expressed specifically in hematopoietic cells and tissues.

    Gene expression databases

    ArrayExpressiQ96HJ5.
    BgeeiQ96HJ5.
    CleanExiHS_MS4A3.
    GenevestigatoriQ96HJ5.

    Organism-specific databases

    HPAiHPA019210.

    Interactioni

    Subunit structurei

    Interacts with CDKN3. Interacts with CDKN3-CDK2 complexes through its binding to CDKN3; this interaction facilitates dissociation of cyclin A from CDKN3-CDK2 complexes.2 Publications

    Protein-protein interaction databases

    BioGridi107370. 2 interactions.
    STRINGi9606.ENSP00000278865.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96HJ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4949CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini71 – 8111ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini103 – 12422CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini146 – 17530ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini197 – 21418CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei50 – 7021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei82 – 10221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei125 – 14521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei176 – 19621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi82 – 876Poly-Phe

    Domaini

    The C-terminal region is required for binding to CDKN3-CDK2 complexes and the modulation of CDKN3 activity.

    Sequence similaritiesi

    Belongs to the MS4A family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46884.
    HOGENOMiHOG000113657.
    HOVERGENiHBG052512.
    InParanoidiQ96HJ5.
    OMAiSTVAMWC.
    OrthoDBiEOG7VHSZF.
    PhylomeDBiQ96HJ5.
    TreeFamiTF335157.

    Family and domain databases

    InterProiIPR007237. CD20-like.
    [Graphical view]
    PfamiPF04103. CD20. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96HJ5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASHEVDNAE LGSASAHGTP GSEAGPEELN TSVYQPIDGS PDYQKAKLQV    50
    LGAIQILNAA MILALGVFLG SLQYPYHFQK HFFFFTFYTG YPIWGAVFFC 100
    SSGTLSVVAG IKPTRTWIQN SFGMNIASAT IALVGTAFLS LNIAVNIQSL 150
    RSCHSSSESP DLCNYMGSIS NGMVSLLLIL TLLELCVTIS TIAMWCNANC 200
    CNSREEISSP PNSV 214
    Length:214
    Mass (Da):22,933
    Last modified:December 1, 2001 - v1
    Checksum:iD30C276213DB8F3D
    GO
    Isoform 2 (identifier: Q96HJ5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         53-98: Missing.

    Show »
    Length:168
    Mass (Da):17,601
    Checksum:iC62AC47B541A634C
    GO
    Isoform 3 (identifier: Q96HJ5-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-123: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:91
    Mass (Da):9,625
    Checksum:i4570C062BADC3B17
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241A → T in AAA62319. (PubMed:7524084)Curated
    Sequence conflicti35 – 351Q → H in AAA62319. (PubMed:7524084)Curated
    Sequence conflicti38 – 381D → N in AAA62319. (PubMed:7524084)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 123123Missing in isoform 3. 1 PublicationVSP_045798Add
    BLAST
    Alternative sequencei53 – 9846Missing in isoform 2. 1 PublicationVSP_007109Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35848 mRNA. Translation: AAA62319.1.
    AY095480 mRNA. Translation: AAM23312.1.
    AY258289 Genomic DNA. Translation: AAP14648.1.
    AP000790 Genomic DNA. No translation available.
    BC008487 mRNA. Translation: AAH08487.1.
    BG494288 mRNA. No translation available.
    CCDSiCCDS31567.1. [Q96HJ5-1]
    CCDS31568.1. [Q96HJ5-2]
    CCDS41651.1. [Q96HJ5-3]
    PIRiI59258.
    RefSeqiNP_001026836.1. NM_001031666.1. [Q96HJ5-3]
    NP_001026979.1. NM_001031809.1. [Q96HJ5-2]
    NP_006129.4. NM_006138.4. [Q96HJ5-1]
    UniGeneiHs.99960.

    Genome annotation databases

    EnsembliENST00000278865; ENSP00000278865; ENSG00000149516. [Q96HJ5-1]
    ENST00000358152; ENSP00000350872; ENSG00000149516. [Q96HJ5-2]
    ENST00000395032; ENSP00000378473; ENSG00000149516. [Q96HJ5-3]
    ENST00000534744; ENSP00000434117; ENSG00000149516. [Q96HJ5-2]
    GeneIDi932.
    KEGGihsa:932.
    UCSCiuc001nom.3. human. [Q96HJ5-1]
    uc001non.3. human. [Q96HJ5-2]

    Polymorphism databases

    DMDMi29611825.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35848 mRNA. Translation: AAA62319.1 .
    AY095480 mRNA. Translation: AAM23312.1 .
    AY258289 Genomic DNA. Translation: AAP14648.1 .
    AP000790 Genomic DNA. No translation available.
    BC008487 mRNA. Translation: AAH08487.1 .
    BG494288 mRNA. No translation available.
    CCDSi CCDS31567.1. [Q96HJ5-1 ]
    CCDS31568.1. [Q96HJ5-2 ]
    CCDS41651.1. [Q96HJ5-3 ]
    PIRi I59258.
    RefSeqi NP_001026836.1. NM_001031666.1. [Q96HJ5-3 ]
    NP_001026979.1. NM_001031809.1. [Q96HJ5-2 ]
    NP_006129.4. NM_006138.4. [Q96HJ5-1 ]
    UniGenei Hs.99960.

    3D structure databases

    ProteinModelPortali Q96HJ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107370. 2 interactions.
    STRINGi 9606.ENSP00000278865.

    Polymorphism databases

    DMDMi 29611825.

    Proteomic databases

    PaxDbi Q96HJ5.
    PRIDEi Q96HJ5.

    Protocols and materials databases

    DNASUi 932.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000278865 ; ENSP00000278865 ; ENSG00000149516 . [Q96HJ5-1 ]
    ENST00000358152 ; ENSP00000350872 ; ENSG00000149516 . [Q96HJ5-2 ]
    ENST00000395032 ; ENSP00000378473 ; ENSG00000149516 . [Q96HJ5-3 ]
    ENST00000534744 ; ENSP00000434117 ; ENSG00000149516 . [Q96HJ5-2 ]
    GeneIDi 932.
    KEGGi hsa:932.
    UCSCi uc001nom.3. human. [Q96HJ5-1 ]
    uc001non.3. human. [Q96HJ5-2 ]

    Organism-specific databases

    CTDi 932.
    GeneCardsi GC11P059824.
    HGNCi HGNC:7317. MS4A3.
    HPAi HPA019210.
    MIMi 606498. gene.
    neXtProti NX_Q96HJ5.
    PharmGKBi PA31112.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG46884.
    HOGENOMi HOG000113657.
    HOVERGENi HBG052512.
    InParanoidi Q96HJ5.
    OMAi STVAMWC.
    OrthoDBi EOG7VHSZF.
    PhylomeDBi Q96HJ5.
    TreeFami TF335157.

    Miscellaneous databases

    GeneWikii MS4A3.
    GenomeRNAii 932.
    NextBioi 3866.
    PROi Q96HJ5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96HJ5.
    Bgeei Q96HJ5.
    CleanExi HS_MS4A3.
    Genevestigatori Q96HJ5.

    Family and domain databases

    InterProi IPR007237. CD20-like.
    [Graphical view ]
    Pfami PF04103. CD20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the cDNA for a hematopoietic cell-specific protein related to CD20 and the beta subunit of the high-affinity IgE receptor: evidence for a family of proteins with four membrane-spanning regions."
      Adra C.N., Lelias J.-M., Kobayashi H., Kaghad M., Morrison P., Rowley J.D., Lim B.
      Proc. Natl. Acad. Sci. U.S.A. 91:10178-10182(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Hematopoietic.
    2. "Cloning and function analysis of the transcript variant of human hematopoietic cell-specific protein HTm4."
      Xie C., Yuan H.F., Xie X.Y., Li Y.H., Shi W., Wang D.M., Li H.M., Yue W., Bai C.X., Zhang R., Pei X.T.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Bone marrow.
    3. Shirakawa T., Yang X., Lyengar A., Sayegh M., Scadden D., Adra C.
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Tissue: Epidermal carcinoma and Lung.
    6. Cited for: FUNCTION, INTERACTION WITH CDKN3.
    7. "Binding of HTm4 to cyclin-dependent kinase (Cdk)-associated phosphatase (KAP).Cdk2.cyclin A complex enhances the phosphatase activity of KAP, dissociates cyclin A, and facilitates KAP dephosphorylation of Cdk2."
      Chinami M., Yano Y., Yang X., Salahuddin S., Moriyama K., Shiroishi M., Turner H., Shirakawa T., Adra C.N.
      J. Biol. Chem. 280:17235-17242(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDKN3.

    Entry informationi

    Entry nameiMS4A3_HUMAN
    AccessioniPrimary (citable) accession number: Q96HJ5
    Secondary accession number(s): A8MTP8, Q8NHW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3