Q96HJ5 (MS4A3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Membrane-spanning 4-domains subfamily A member 3

Alternative name(s):
CD20 antigen-like protein
Hematopoietic-specific transmembrane protein 4
Short name=HTm4

Gene names

Name:	MS4A3
Synonyms:	CD20L, HTM4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hematopoietic modulator for the G1-S cell cycle transition. Modulates the level of phosphorylation of cyclin-dependent kinase 2 (CDK2) through its direct binding to cyclin-dependent kinase inhibitor 3 (CDKN3/KAP). Ref.6
Subunit structure	Interacts with CDKN3. Interacts with CDKN3-CDK2 complexes through its binding to CDKN3; this interaction facilitates dissociation of cyclin A from CDKN3-CDK2 complexes. Ref.6 Ref.7
Subcellular location	Endomembrane system; Multi-pass membrane protein By similarity. Cytoplasm › perinuclear region By similarity. Note: Located in the perinuclear area.
Tissue specificity	Expressed specifically in hematopoietic cells and tissues.
Domain	The C-terminal region is required for binding to CDKN3-CDK2 complexes and the modulation of CDKN3 activity.
Sequence similarities	Belongs to the MS4A family.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Receptor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96HJ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96HJ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     53-98: Missing.

Isoform 3 (identifier: Q96HJ5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Membrane-spanning 4-domains subfamily A member 3		PRO_0000158632
Regions
	Topological domain	1 – 49	49	Cytoplasmic Potential
	Transmembrane	50 – 70	21	Helical; Potential
	Topological domain	71 – 81	11	Extracellular Potential
	Transmembrane	82 – 102	21	Helical; Potential
	Topological domain	103 – 124	22	Cytoplasmic Potential
	Transmembrane	125 – 145	21	Helical; Potential
	Topological domain	146 – 175	30	Extracellular Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Topological domain	197 – 214	18	Cytoplasmic Potential
	Compositional bias	82 – 87	6	Poly-Phe
Natural variations
	Alternative sequence	1 – 123	123	Missing in isoform 3.		VSP_045798
	Alternative sequence	53 – 98	46	Missing in isoform 2.		VSP_007109
Experimental info
	Sequence conflict	24	1	A → T in AAA62319. Ref.1
	Sequence conflict	35	1	Q → H in AAA62319. Ref.1
	Sequence conflict	38	1	D → N in AAA62319. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D30C276213DB8F3D	FASTA	214	22,933
	10 20 30 40 50 60 MASHEVDNAE LGSASAHGTP GSEAGPEELN TSVYQPIDGS PDYQKAKLQV LGAIQILNAA 70 80 90 100 110 120 MILALGVFLG SLQYPYHFQK HFFFFTFYTG YPIWGAVFFC SSGTLSVVAG IKPTRTWIQN 130 140 150 160 170 180 SFGMNIASAT IALVGTAFLS LNIAVNIQSL RSCHSSSESP DLCNYMGSIS NGMVSLLLIL 190 200 210 TLLELCVTIS TIAMWCNANC CNSREEISSP PNSV
	Isoform 2 [UniParc]. Checksum: C62AC47B541A634C	FASTA	168	17,601
	10 20 30 40 50 60 MASHEVDNAE LGSASAHGTP GSEAGPEELN TSVYQPIDGS PDYQKAKLQV LGFCSSGTLS 70 80 90 100 110 120 VVAGIKPTRT WIQNSFGMNI ASATIALVGT AFLSLNIAVN IQSLRSCHSS SESPDLCNYM 130 140 150 160 GSISNGMVSL LLILTLLELC VTISTIAMWC NANCCNSREE ISSPPNSV
	Isoform 3 [UniParc]. Checksum: 4570C062BADC3B17	FASTA	91	9,625
	10 20 30 40 50 60 MNIASATIAL VGTAFLSLNI AVNIQSLRSC HSSSESPDLC NYMGSISNGM VSLLLILTLL 70 80 90 ELCVTISTIA MWCNANCCNS REEISSPPNS V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the cDNA for a hematopoietic cell-specific protein related to CD20 and the beta subunit of the high-affinity IgE receptor: evidence for a family of proteins with four membrane-spanning regions." Adra C.N., Lelias J.-M., Kobayashi H., Kaghad M., Morrison P., Rowley J.D., Lim B. Proc. Natl. Acad. Sci. U.S.A. 91:10178-10182(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Hematopoietic.
[2]	"Cloning and function analysis of the transcript variant of human hematopoietic cell-specific protein HTm4." Xie C., Yuan H.F., Xie X.Y., Li Y.H., Shi W., Wang D.M., Li H.M., Yue W., Bai C.X., Zhang R., Pei X.T. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Bone marrow.
[3]	Shirakawa T., Yang X., Lyengar A., Sayegh M., Scadden D., Adra C. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Epidermal carcinoma and Lung.
[6]	"Human HTm4 is a hematopoietic cell cycle regulator." Donato J.-L., Ko J., Kutok J.L., Cheng T., Shirakawa T., Mao X.-Q., Beach D., Scadden D.T., Sayegh M.H., Adra C.N. J. Clin. Invest. 109:51-58(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDKN3.
[7]	"Binding of HTm4 to cyclin-dependent kinase (Cdk)-associated phosphatase (KAP).Cdk2.cyclin A complex enhances the phosphatase activity of KAP, dissociates cyclin A, and facilitates KAP dephosphorylation of Cdk2." Chinami M., Yano Y., Yang X., Salahuddin S., Moriyama K., Shiroishi M., Turner H., Shirakawa T., Adra C.N. J. Biol. Chem. 280:17235-17242(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CDKN3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L35848 mRNA. Translation: AAA62319.1. AY095480 mRNA. Translation: AAM23312.1. AY258289 Genomic DNA. Translation: AAP14648.1. AP000790 Genomic DNA. No translation available. BC008487 mRNA. Translation: AAH08487.1. BG494288 mRNA. No translation available.
IPI	IPI00220897. IPI00220913. IPI00642885.
PIR	I59258.
RefSeq	NP_001026836.1. NM_001031666.1. NP_001026979.1. NM_001031809.1. NP_006129.4. NM_006138.4.
UniGene	Hs.99960.
3D structure databases
ProteinModelPortal	Q96HJ5.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000278865.
Polymorphism databases
DMDM	29611825.
Proteomic databases
PaxDb	Q96HJ5.
PRIDE	Q96HJ5.
Protocols and materials databases
DNASU	932.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000278865; ENSP00000278865; ENSG00000149516. ENST00000358152; ENSP00000350872; ENSG00000149516. ENST00000395032; ENSP00000378473; ENSG00000149516. ENST00000534744; ENSP00000434117; ENSG00000149516.
GeneID	932.
KEGG	hsa:932.
UCSC	uc001nom.3. human. uc001non.3. human.
Organism-specific databases
CTD	932.
GeneCards	GC11P059824.
HGNC	HGNC:7317. MS4A3.
HPA	HPA019210.
MIM	606498. gene.
neXtProt	NX_Q96HJ5.
PharmGKB	PA31112.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG46884.
HOGENOM	HOG000113657.
HOVERGEN	HBG052512.
InParanoid	Q96HJ5.
OMA	CCNSREE.
OrthoDB	EOG4MPHR7.
PhylomeDB	Q96HJ5.
Gene expression databases
ArrayExpress	Q96HJ5.
Bgee	Q96HJ5.
CleanEx	HS_MS4A3.
Genevestigator	Q96HJ5.
GermOnline	ENSG00000149516. Homo sapiens.
Family and domain databases
InterPro	IPR007237. CD20-like. [Graphical view]
Pfam	PF04103. CD20. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	932.
NextBio	3866.
SOURCE	Search...

Entry information

Entry name

MS4A3_HUMAN

Accession

Primary (citable) accession number: Q96HJ5
Secondary accession number(s): A8MTP8, Q8NHW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.