Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q96HJ5 (MS4A3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Membrane-spanning 4-domains subfamily A member 3
Alternative name(s):
CD20 antigen-like protein
Hematopoietic-specific transmembrane protein 4
Short name=HTm4
Gene names
Name:MS4A3
Synonyms:CD20L, HTM4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hematopoietic modulator for the G1-S cell cycle transition. Modulates the level of phosphorylation of cyclin-dependent kinase 2 (CDK2) through its direct binding to cyclin-dependent kinase inhibitor 3 (CDKN3/KAP). Ref.6

Subunit structure

Interacts with CDKN3. Interacts with CDKN3-CDK2 complexes through its binding to CDKN3; this interaction facilitates dissociation of cyclin A from CDKN3-CDK2 complexes. Ref.6 Ref.7

Subcellular location

Endomembrane system; Multi-pass membrane protein By similarity. Cytoplasmperinuclear region By similarity. Note: Located in the perinuclear area.

Tissue specificity

Expressed specifically in hematopoietic cells and tissues.

Domain

The C-terminal region is required for binding to CDKN3-CDK2 complexes and the modulation of CDKN3 activity.

Sequence similarities

Belongs to the MS4A family.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Molecular functionReceptor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentendomembrane system

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction Ref.7. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96HJ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96HJ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     53-98: Missing.
Isoform 3 (identifier: Q96HJ5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 214214Membrane-spanning 4-domains subfamily A member 3
PRO_0000158632

Regions

Topological domain1 – 4949Cytoplasmic Potential
Transmembrane50 – 7021Helical; Potential
Topological domain71 – 8111Extracellular Potential
Transmembrane82 – 10221Helical; Potential
Topological domain103 – 12422Cytoplasmic Potential
Transmembrane125 – 14521Helical; Potential
Topological domain146 – 17530Extracellular Potential
Transmembrane176 – 19621Helical; Potential
Topological domain197 – 21418Cytoplasmic Potential
Compositional bias82 – 876Poly-Phe

Natural variations

Alternative sequence1 – 123123Missing in isoform 3.
VSP_045798
Alternative sequence53 – 9846Missing in isoform 2.
VSP_007109

Experimental info

Sequence conflict241A → T in AAA62319. Ref.1
Sequence conflict351Q → H in AAA62319. Ref.1
Sequence conflict381D → N in AAA62319. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: D30C276213DB8F3D

FASTA21422,933
        10         20         30         40         50         60 
MASHEVDNAE LGSASAHGTP GSEAGPEELN TSVYQPIDGS PDYQKAKLQV LGAIQILNAA 

        70         80         90        100        110        120 
MILALGVFLG SLQYPYHFQK HFFFFTFYTG YPIWGAVFFC SSGTLSVVAG IKPTRTWIQN 

       130        140        150        160        170        180 
SFGMNIASAT IALVGTAFLS LNIAVNIQSL RSCHSSSESP DLCNYMGSIS NGMVSLLLIL 

       190        200        210 
TLLELCVTIS TIAMWCNANC CNSREEISSP PNSV 

« Hide

Isoform 2 [UniParc].

Checksum: C62AC47B541A634C
Show »

FASTA16817,601
Isoform 3 [UniParc].

Checksum: 4570C062BADC3B17
Show »

FASTA919,625

References

« Hide 'large scale' references
[1]"Cloning of the cDNA for a hematopoietic cell-specific protein related to CD20 and the beta subunit of the high-affinity IgE receptor: evidence for a family of proteins with four membrane-spanning regions."
Adra C.N., Lelias J.-M., Kobayashi H., Kaghad M., Morrison P., Rowley J.D., Lim B.
Proc. Natl. Acad. Sci. U.S.A. 91:10178-10182(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Hematopoietic.
[2]"Cloning and function analysis of the transcript variant of human hematopoietic cell-specific protein HTm4."
Xie C., Yuan H.F., Xie X.Y., Li Y.H., Shi W., Wang D.M., Li H.M., Yue W., Bai C.X., Zhang R., Pei X.T.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Bone marrow.
[3]Shirakawa T., Yang X., Lyengar A., Sayegh M., Scadden D., Adra C.
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Epidermal carcinoma and Lung.
[6]"Human HTm4 is a hematopoietic cell cycle regulator."
Donato J.-L., Ko J., Kutok J.L., Cheng T., Shirakawa T., Mao X.-Q., Beach D., Scadden D.T., Sayegh M.H., Adra C.N.
J. Clin. Invest. 109:51-58(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDKN3.
[7]"Binding of HTm4 to cyclin-dependent kinase (Cdk)-associated phosphatase (KAP).Cdk2.cyclin A complex enhances the phosphatase activity of KAP, dissociates cyclin A, and facilitates KAP dephosphorylation of Cdk2."
Chinami M., Yano Y., Yang X., Salahuddin S., Moriyama K., Shiroishi M., Turner H., Shirakawa T., Adra C.N.
J. Biol. Chem. 280:17235-17242(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CDKN3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L35848 mRNA. Translation: AAA62319.1.
AY095480 mRNA. Translation: AAM23312.1.
AY258289 Genomic DNA. Translation: AAP14648.1.
AP000790 Genomic DNA. No translation available.
BC008487 mRNA. Translation: AAH08487.1.
BG494288 mRNA. No translation available.
CCDSCCDS31567.1. [Q96HJ5-1]
CCDS31568.1. [Q96HJ5-2]
CCDS41651.1. [Q96HJ5-3]
PIRI59258.
RefSeqNP_001026836.1. NM_001031666.1. [Q96HJ5-3]
NP_001026979.1. NM_001031809.1. [Q96HJ5-2]
NP_006129.4. NM_006138.4. [Q96HJ5-1]
UniGeneHs.99960.

3D structure databases

ProteinModelPortalQ96HJ5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107370. 2 interactions.
STRING9606.ENSP00000278865.

Polymorphism databases

DMDM29611825.

Proteomic databases

PaxDbQ96HJ5.
PRIDEQ96HJ5.

Protocols and materials databases

DNASU932.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000278865; ENSP00000278865; ENSG00000149516. [Q96HJ5-1]
ENST00000358152; ENSP00000350872; ENSG00000149516. [Q96HJ5-2]
ENST00000395032; ENSP00000378473; ENSG00000149516. [Q96HJ5-3]
ENST00000534744; ENSP00000434117; ENSG00000149516. [Q96HJ5-2]
GeneID932.
KEGGhsa:932.
UCSCuc001nom.3. human. [Q96HJ5-1]
uc001non.3. human. [Q96HJ5-2]

Organism-specific databases

CTD932.
GeneCardsGC11P059824.
HGNCHGNC:7317. MS4A3.
HPAHPA019210.
MIM606498. gene.
neXtProtNX_Q96HJ5.
PharmGKBPA31112.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG46884.
HOGENOMHOG000113657.
HOVERGENHBG052512.
InParanoidQ96HJ5.
OMASTVAMWC.
OrthoDBEOG7VHSZF.
PhylomeDBQ96HJ5.
TreeFamTF335157.

Gene expression databases

ArrayExpressQ96HJ5.
BgeeQ96HJ5.
CleanExHS_MS4A3.
GenevestigatorQ96HJ5.

Family and domain databases

InterProIPR007237. CD20-like.
[Graphical view]
PfamPF04103. CD20. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMS4A3.
GenomeRNAi932.
NextBio3866.
PROQ96HJ5.
SOURCESearch...

Entry information

Entry nameMS4A3_HUMAN
AccessionPrimary (citable) accession number: Q96HJ5
Secondary accession number(s): A8MTP8, Q8NHW2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2003
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM