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Q96HI0

- SENP5_HUMAN

UniProt

Q96HI0 - SENP5_HUMAN

Protein

Sentrin-specific protease 5

Gene

SENP5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 3 (18 May 2010)
      Previous versions | rss
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    Functioni

    Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division.2 Publications

    Catalytic activityi

    Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei646 – 6461By similarity
    Active sitei663 – 6631By similarity
    Active sitei713 – 7131Curated

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. cellular protein metabolic process Source: Reactome
    4. post-translational protein modification Source: Reactome
    5. protein sumoylation Source: Reactome

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Cell cycle, Cell division, Ubl conjugation pathway

    Enzyme and pathway databases

    BRENDAi3.4.22.68. 2681.
    ReactomeiREACT_163725. SUMO is proteolytically processed.

    Protein family/group databases

    MEROPSiC48.008.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sentrin-specific protease 5 (EC:3.4.22.68)
    Alternative name(s):
    Sentrin/SUMO-specific protease SENP5
    Gene namesi
    Name:SENP5
    ORF Names:FKSG45
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:28407. SENP5.

    Subcellular locationi

    Nucleusnucleolus 2 Publications

    GO - Cellular componenti

    1. intracellular membrane-bounded organelle Source: HPA
    2. nucleolus Source: HPA
    3. nucleoplasm Source: Reactome
    4. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi713 – 7131C → A: Abolishes enzymatic activity. 2 Publications

    Organism-specific databases

    PharmGKBiPA134917083.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 755755Sentrin-specific protease 5PRO_0000101723Add
    BLAST

    Proteomic databases

    MaxQBiQ96HI0.
    PaxDbiQ96HI0.
    PRIDEiQ96HI0.

    PTM databases

    PhosphoSiteiQ96HI0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ96HI0.
    BgeeiQ96HI0.
    CleanExiHS_SENP5.
    GenevestigatoriQ96HI0.

    Organism-specific databases

    HPAiHPA055536.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ESR1P033722EBI-3895753,EBI-78473

    Protein-protein interaction databases

    BioGridi128497. 2 interactions.
    IntActiQ96HI0. 1 interaction.
    STRINGi9606.ENSP00000327197.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96HI0.
    SMRiQ96HI0. Positions 515-754.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni567 – 724158ProteaseAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C48 family.Curated

    Phylogenomic databases

    eggNOGiCOG5160.
    HOGENOMiHOG000070166.
    HOVERGENiHBG062231.
    InParanoidiQ96HI0.
    KOiK08594.
    OMAiAFPDQQN.
    OrthoDBiEOG7D59MP.
    PhylomeDBiQ96HI0.
    TreeFamiTF316289.

    Family and domain databases

    InterProiIPR003653. Peptidase_C48.
    [Graphical view]
    PfamiPF02902. Peptidase_C48. 1 hit.
    [Graphical view]
    PROSITEiPS50600. ULP_PROTEASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96HI0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKKQRKILWR KGIHLAFSEK WNTGFGGFKK FYFHQHLCIL KAKLGRPVTW    50
    NRQLRHFQGR KKALQIQKTW IKDEPLCAKT KFNVATQNVS TLSSKVKRKD 100
    AKHFISSSKT LLRLQAEKLL SSAKNSDHEY CREKNLLKAV TDFPSNSALG 150
    QANGHRPRTD PQPSDFPMKF NGESQSPGES GTIVVTLNNH KRKGFCYGCC 200
    QGPEHHRNGG PLIPKKFQLN QHRRIKLSPL MMYEKLSMIR FRYRILRSQH 250
    FRTKSKVCKL RKAQRSWVQK VTGDHQETRR ENGEGGSCSP FPSPEPKDPS 300
    CRHQPYFPDM DSSAVVKGTN SHVPDCHTKG SSFLGKELSL DEAFPDQQNG 350
    SATNAWDQSS CSSPKWECTE LIHDIPLPEH RSNTMFISET EREIMTLGQE 400
    NQTSSVSDDR VKLSVSGADT SVSSVDGPVS QKAVQNENSY QMEEDGSLKQ 450
    SILSSELLDH PYCKSPLEAP LVCSGLKLEN QVGGGKNSQK ASPVDDEQLS 500
    VCLSGFLDEV MKKYGSLVPL SEKEVLGRLK DVFNEDFSNR KPFINREITN 550
    YRARHQKCNF RIFYNKHMLD MDDLATLDGQ NWLNDQVINM YGELIMDAVP 600
    DKVHFFNSFF HRQLVTKGYN GVKRWTKKVD LFKKSLLLIP IHLEVHWSLI 650
    TVTLSNRIIS FYDSQGIHFK FCVENIRKYL LTEAREKNRP EFLQGWQTAV 700
    TKCIPQQKND SDCGVFVLQY CKCLALEQPF QFSQEDMPRV RKRIYKELCE 750
    CRLMD 755
    Length:755
    Mass (Da):86,693
    Last modified:May 18, 2010 - v3
    Checksum:i90D7B34B2853CEE4
    GO
    Isoform 2 (identifier: Q96HI0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         629-674: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:709
    Mass (Da):81,310
    Checksum:iBA608D50017296EC
    GO

    Sequence cautioni

    The sequence AAK69630.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti75 – 751P → H in AAH30705. (PubMed:15489334)Curated
    Sequence conflicti538 – 5381S → C in AAK69630. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti83 – 831N → S.
    Corresponds to variant rs35434690 [ dbSNP | Ensembl ].
    VAR_057045
    Natural varianti340 – 3401L → F.
    Corresponds to variant rs34251880 [ dbSNP | Ensembl ].
    VAR_061732

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei629 – 67446Missing in isoform 2. 1 PublicationVSP_056415Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK302305 mRNA. Translation: BAG63644.1.
    AC011322 Genomic DNA. No translation available.
    AC016949 Genomic DNA. No translation available.
    AC127904 Genomic DNA. No translation available.
    BC008589 mRNA. Translation: AAH08589.1.
    BC030705 mRNA. Translation: AAH30705.1.
    AF335474 mRNA. Translation: AAK69630.1. Different initiation.
    CCDSiCCDS3322.1.
    RefSeqiNP_689912.2. NM_152699.4.
    XP_005269368.1. XM_005269311.1.
    UniGeneiHs.240770.

    Genome annotation databases

    EnsembliENST00000323460; ENSP00000327197; ENSG00000119231.
    ENST00000445299; ENSP00000390231; ENSG00000119231.
    GeneIDi205564.
    KEGGihsa:205564.
    UCSCiuc003fwz.4. human.

    Polymorphism databases

    DMDMi296452962.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK302305 mRNA. Translation: BAG63644.1 .
    AC011322 Genomic DNA. No translation available.
    AC016949 Genomic DNA. No translation available.
    AC127904 Genomic DNA. No translation available.
    BC008589 mRNA. Translation: AAH08589.1 .
    BC030705 mRNA. Translation: AAH30705.1 .
    AF335474 mRNA. Translation: AAK69630.1 . Different initiation.
    CCDSi CCDS3322.1.
    RefSeqi NP_689912.2. NM_152699.4.
    XP_005269368.1. XM_005269311.1.
    UniGenei Hs.240770.

    3D structure databases

    ProteinModelPortali Q96HI0.
    SMRi Q96HI0. Positions 515-754.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 128497. 2 interactions.
    IntActi Q96HI0. 1 interaction.
    STRINGi 9606.ENSP00000327197.

    Protein family/group databases

    MEROPSi C48.008.

    PTM databases

    PhosphoSitei Q96HI0.

    Polymorphism databases

    DMDMi 296452962.

    Proteomic databases

    MaxQBi Q96HI0.
    PaxDbi Q96HI0.
    PRIDEi Q96HI0.

    Protocols and materials databases

    DNASUi 205564.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000323460 ; ENSP00000327197 ; ENSG00000119231 .
    ENST00000445299 ; ENSP00000390231 ; ENSG00000119231 .
    GeneIDi 205564.
    KEGGi hsa:205564.
    UCSCi uc003fwz.4. human.

    Organism-specific databases

    CTDi 205564.
    GeneCardsi GC03P196594.
    H-InvDB HIX0018042.
    HGNCi HGNC:28407. SENP5.
    HPAi HPA055536.
    MIMi 612845. gene.
    neXtProti NX_Q96HI0.
    PharmGKBi PA134917083.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5160.
    HOGENOMi HOG000070166.
    HOVERGENi HBG062231.
    InParanoidi Q96HI0.
    KOi K08594.
    OMAi AFPDQQN.
    OrthoDBi EOG7D59MP.
    PhylomeDBi Q96HI0.
    TreeFami TF316289.

    Enzyme and pathway databases

    BRENDAi 3.4.22.68. 2681.
    Reactomei REACT_163725. SUMO is proteolytically processed.

    Miscellaneous databases

    ChiTaRSi SENP5. human.
    GenomeRNAii 205564.
    NextBioi 90512.
    PROi Q96HI0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96HI0.
    Bgeei Q96HI0.
    CleanExi HS_SENP5.
    Genevestigatori Q96HI0.

    Family and domain databases

    InterProi IPR003653. Peptidase_C48.
    [Graphical view ]
    Pfami PF02902. Peptidase_C48. 1 hit.
    [Graphical view ]
    PROSITEi PS50600. ULP_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Testis.
    2. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta and Testis.
    4. "Identification of FKSG45, a novel gene located on human chromosome 3."
      Wang Y.-G., Li T.
      Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 305-755 (ISOFORM 1).
    5. "Ubiquitin-like proteins: new wines in new bottles."
      Yeh E.T.H., Gong L., Kamitani T.
      Gene 248:1-14(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    6. "Characterization of a family of nucleolar SUMO-specific proteases with preference for SUMO-2 or SUMO-3."
      Gong L., Yeh E.T.H.
      J. Biol. Chem. 281:15869-15877(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-713.
    7. "The SUMO-specific protease SENP5 is required for cell division."
      Di Bacco A., Ouyang J., Lee H.-Y., Catic A., Ploegh H., Gill G.
      Mol. Cell. Biol. 26:4489-4498(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-713.
    8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.

    Entry informationi

    Entry nameiSENP5_HUMAN
    AccessioniPrimary (citable) accession number: Q96HI0
    Secondary accession number(s): B4DY82, Q96SA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2002
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 103 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3