Q96HI0 (SENP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sentrin-specific protease 5
EC=3.4.22.68

Alternative name(s):
Sentrin/SUMO-specific protease SENP5

Gene names

Name:	SENP5
ORF Names:	FKSG45

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	755 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division. Ref.5 Ref.6
Catalytic activity	Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.
Subcellular location	Nucleus › nucleolus Ref.5 Ref.6.
Sequence similarities	Belongs to the peptidase C48 family.
Sequence caution	The sequence AAK69630.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Cell cycle Cell division Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ESR1	P03372	2	EBI-3895753,EBI-78473

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 755	755	Sentrin-specific protease 5		PRO_0000101723
Regions
	Region	567 – 724	158	Protease
Sites
	Active site	646	1	By similarity
	Active site	663	1	By similarity
	Active site	713	1	Probable
Natural variations
	Natural variant	83	1	N → S. Corresponds to variant rs35434690 [ dbSNP | Ensembl ].		VAR_057045
	Natural variant	340	1	L → F. Corresponds to variant rs34251880 [ dbSNP | Ensembl ].		VAR_061732
Experimental info
	Mutagenesis	713	1	C → A: Abolishes enzymatic activity. Ref.5 Ref.6
	Sequence conflict	75	1	P → H in AAH30705. Ref.2
	Sequence conflict	538	1	S → C in AAK69630. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q96HI0 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: 90D7B34B2853CEE4	FASTA	755	86,693
	10 20 30 40 50 60 MKKQRKILWR KGIHLAFSEK WNTGFGGFKK FYFHQHLCIL KAKLGRPVTW NRQLRHFQGR 70 80 90 100 110 120 KKALQIQKTW IKDEPLCAKT KFNVATQNVS TLSSKVKRKD AKHFISSSKT LLRLQAEKLL 130 140 150 160 170 180 SSAKNSDHEY CREKNLLKAV TDFPSNSALG QANGHRPRTD PQPSDFPMKF NGESQSPGES 190 200 210 220 230 240 GTIVVTLNNH KRKGFCYGCC QGPEHHRNGG PLIPKKFQLN QHRRIKLSPL MMYEKLSMIR 250 260 270 280 290 300 FRYRILRSQH FRTKSKVCKL RKAQRSWVQK VTGDHQETRR ENGEGGSCSP FPSPEPKDPS 310 320 330 340 350 360 CRHQPYFPDM DSSAVVKGTN SHVPDCHTKG SSFLGKELSL DEAFPDQQNG SATNAWDQSS 370 380 390 400 410 420 CSSPKWECTE LIHDIPLPEH RSNTMFISET EREIMTLGQE NQTSSVSDDR VKLSVSGADT 430 440 450 460 470 480 SVSSVDGPVS QKAVQNENSY QMEEDGSLKQ SILSSELLDH PYCKSPLEAP LVCSGLKLEN 490 500 510 520 530 540 QVGGGKNSQK ASPVDDEQLS VCLSGFLDEV MKKYGSLVPL SEKEVLGRLK DVFNEDFSNR 550 560 570 580 590 600 KPFINREITN YRARHQKCNF RIFYNKHMLD MDDLATLDGQ NWLNDQVINM YGELIMDAVP 610 620 630 640 650 660 DKVHFFNSFF HRQLVTKGYN GVKRWTKKVD LFKKSLLLIP IHLEVHWSLI TVTLSNRIIS 670 680 690 700 710 720 FYDSQGIHFK FCVENIRKYL LTEAREKNRP EFLQGWQTAV TKCIPQQKND SDCGVFVLQY 730 740 750 CKCLALEQPF QFSQEDMPRV RKRIYKELCE CRLMD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and Testis.
[3]	"Identification of FKSG45, a novel gene located on human chromosome 3." Wang Y.-G., Li T. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 305-755.
[4]	"Ubiquitin-like proteins: new wines in new bottles." Yeh E.T.H., Gong L., Kamitani T. Gene 248:1-14(2000) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[5]	"Characterization of a family of nucleolar SUMO-specific proteases with preference for SUMO-2 or SUMO-3." Gong L., Yeh E.T.H. J. Biol. Chem. 281:15869-15877(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-713.
[6]	"The SUMO-specific protease SENP5 is required for cell division." Di Bacco A., Ouyang J., Lee H.-Y., Catic A., Ploegh H., Gill G. Mol. Cell. Biol. 26:4489-4498(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-713.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC011322 Genomic DNA. No translation available. AC016949 Genomic DNA. No translation available. AC127904 Genomic DNA. No translation available. BC008589 mRNA. Translation: AAH08589.1. BC030705 mRNA. Translation: AAH30705.1. AF335474 mRNA. Translation: AAK69630.1. Different initiation.
IPI	IPI00063106.
RefSeq	NP_689912.2. NM_152699.4.
UniGene	Hs.240770.
3D structure databases
ProteinModelPortal	Q96HI0.
SMR	Q96HI0. Positions 515-754.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96HI0. 1 interaction.
STRING	9606.ENSP00000327197.
Protein family/group databases
MEROPS	C48.008.
PTM databases
PhosphoSite	Q96HI0.
Polymorphism databases
DMDM	296452962.
Proteomic databases
PaxDb	Q96HI0.
PRIDE	Q96HI0.
Protocols and materials databases
DNASU	205564.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000323460; ENSP00000327197; ENSG00000119231.
GeneID	205564.
KEGG	hsa:205564.
UCSC	uc003fwz.4. human.
Organism-specific databases
CTD	205564.
GeneCards	GC03P196594.
H-InvDB	HIX0018042.
HGNC	HGNC:28407. SENP5.
MIM	612845. gene.
neXtProt	NX_Q96HI0.
PharmGKB	PA134917083.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5160.
HOGENOM	HOG000070166.
HOVERGEN	HBG062231.
InParanoid	Q96HI0.
KO	K08594.
OMA	CKSPLEA.
OrthoDB	EOG479F7X.
PhylomeDB	Q96HI0.
Enzyme and pathway databases
BRENDA	3.4.22.68. 2681.
Gene expression databases
ArrayExpress	Q96HI0.
Bgee	Q96HI0.
CleanEx	HS_SENP5.
Genevestigator	Q96HI0.
GermOnline	ENSG00000119231. Homo sapiens.
Family and domain databases
InterPro	IPR003653. Peptidase_C48. [Graphical view]
Pfam	PF02902. Peptidase_C48. 1 hit. [Graphical view]
PROSITE	PS50600. ULP_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SENP5. human.
GenomeRNAi	205564.
NextBio	90512.
SOURCE	Search...

Entry information

Entry name

SENP5_HUMAN

Accession

Primary (citable) accession number: Q96HI0
Secondary accession number(s): Q96SA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.