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Q96HI0 (SENP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sentrin-specific protease 5

EC=3.4.22.68
Alternative name(s):
Sentrin/SUMO-specific protease SENP5
Gene names
Name:SENP5
ORF Names:FKSG45
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length755 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division. Ref.5 Ref.6

Catalytic activity

Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.

Subcellular location

Nucleusnucleolus Ref.5 Ref.6.

Sequence similarities

Belongs to the peptidase C48 family.

Sequence caution

The sequence AAK69630.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ESR1P033722EBI-3895753,EBI-78473

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 755755Sentrin-specific protease 5
PRO_0000101723

Regions

Region567 – 724158Protease

Sites

Active site6461 By similarity
Active site6631 By similarity
Active site7131 Probable

Natural variations

Natural variant831N → S.
Corresponds to variant rs35434690 [ dbSNP | Ensembl ].
VAR_057045
Natural variant3401L → F.
Corresponds to variant rs34251880 [ dbSNP | Ensembl ].
VAR_061732

Experimental info

Mutagenesis7131C → A: Abolishes enzymatic activity. Ref.5 Ref.6
Sequence conflict751P → H in AAH30705. Ref.2
Sequence conflict5381S → C in AAK69630. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q96HI0 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 90D7B34B2853CEE4

FASTA75586,693
        10         20         30         40         50         60 
MKKQRKILWR KGIHLAFSEK WNTGFGGFKK FYFHQHLCIL KAKLGRPVTW NRQLRHFQGR 

        70         80         90        100        110        120 
KKALQIQKTW IKDEPLCAKT KFNVATQNVS TLSSKVKRKD AKHFISSSKT LLRLQAEKLL 

       130        140        150        160        170        180 
SSAKNSDHEY CREKNLLKAV TDFPSNSALG QANGHRPRTD PQPSDFPMKF NGESQSPGES 

       190        200        210        220        230        240 
GTIVVTLNNH KRKGFCYGCC QGPEHHRNGG PLIPKKFQLN QHRRIKLSPL MMYEKLSMIR 

       250        260        270        280        290        300 
FRYRILRSQH FRTKSKVCKL RKAQRSWVQK VTGDHQETRR ENGEGGSCSP FPSPEPKDPS 

       310        320        330        340        350        360 
CRHQPYFPDM DSSAVVKGTN SHVPDCHTKG SSFLGKELSL DEAFPDQQNG SATNAWDQSS 

       370        380        390        400        410        420 
CSSPKWECTE LIHDIPLPEH RSNTMFISET EREIMTLGQE NQTSSVSDDR VKLSVSGADT 

       430        440        450        460        470        480 
SVSSVDGPVS QKAVQNENSY QMEEDGSLKQ SILSSELLDH PYCKSPLEAP LVCSGLKLEN 

       490        500        510        520        530        540 
QVGGGKNSQK ASPVDDEQLS VCLSGFLDEV MKKYGSLVPL SEKEVLGRLK DVFNEDFSNR 

       550        560        570        580        590        600 
KPFINREITN YRARHQKCNF RIFYNKHMLD MDDLATLDGQ NWLNDQVINM YGELIMDAVP 

       610        620        630        640        650        660 
DKVHFFNSFF HRQLVTKGYN GVKRWTKKVD LFKKSLLLIP IHLEVHWSLI TVTLSNRIIS 

       670        680        690        700        710        720 
FYDSQGIHFK FCVENIRKYL LTEAREKNRP EFLQGWQTAV TKCIPQQKND SDCGVFVLQY 

       730        740        750 
CKCLALEQPF QFSQEDMPRV RKRIYKELCE CRLMD 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta and Testis.
[3]"Identification of FKSG45, a novel gene located on human chromosome 3."
Wang Y.-G., Li T.
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 305-755.
[4]"Ubiquitin-like proteins: new wines in new bottles."
Yeh E.T.H., Gong L., Kamitani T.
Gene 248:1-14(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[5]"Characterization of a family of nucleolar SUMO-specific proteases with preference for SUMO-2 or SUMO-3."
Gong L., Yeh E.T.H.
J. Biol. Chem. 281:15869-15877(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-713.
[6]"The SUMO-specific protease SENP5 is required for cell division."
Di Bacco A., Ouyang J., Lee H.-Y., Catic A., Ploegh H., Gill G.
Mol. Cell. Biol. 26:4489-4498(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-713.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC011322 Genomic DNA. No translation available.
AC016949 Genomic DNA. No translation available.
AC127904 Genomic DNA. No translation available.
BC008589 mRNA. Translation: AAH08589.1.
BC030705 mRNA. Translation: AAH30705.1.
AF335474 mRNA. Translation: AAK69630.1. Different initiation.
RefSeqNP_689912.2. NM_152699.4.
UniGeneHs.240770.

3D structure databases

ProteinModelPortalQ96HI0.
SMRQ96HI0. Positions 515-754.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid128497. 1 interaction.
IntActQ96HI0. 1 interaction.
STRING9606.ENSP00000327197.

Protein family/group databases

MEROPSC48.008.

PTM databases

PhosphoSiteQ96HI0.

Polymorphism databases

DMDM296452962.

Proteomic databases

PaxDbQ96HI0.
PRIDEQ96HI0.

Protocols and materials databases

DNASU205564.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000323460; ENSP00000327197; ENSG00000119231.
GeneID205564.
KEGGhsa:205564.
UCSCuc003fwz.4. human.

Organism-specific databases

CTD205564.
GeneCardsGC03P196594.
H-InvDBHIX0018042.
HGNCHGNC:28407. SENP5.
HPAHPA055536.
MIM612845. gene.
neXtProtNX_Q96HI0.
PharmGKBPA134917083.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5160.
HOGENOMHOG000070166.
HOVERGENHBG062231.
InParanoidQ96HI0.
KOK08594.
OMAAFPDQQN.
OrthoDBEOG7D59MP.
PhylomeDBQ96HI0.
TreeFamTF316289.

Enzyme and pathway databases

BRENDA3.4.22.68. 2681.
ReactomeREACT_17015. Metabolism of proteins.

Gene expression databases

ArrayExpressQ96HI0.
BgeeQ96HI0.
CleanExHS_SENP5.
GenevestigatorQ96HI0.

Family and domain databases

InterProIPR003653. Peptidase_C48.
[Graphical view]
PfamPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSENP5. human.
GenomeRNAi205564.
NextBio90512.
PROQ96HI0.
SOURCESearch...

Entry information

Entry nameSENP5_HUMAN
AccessionPrimary (citable) accession number: Q96HI0
Secondary accession number(s): Q96SA5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM