Reviewed, UniProtKB/Swiss-Prot Q96HI0 (SENP5_HUMAN)

Names and origin

Protein names

Recommended name:
    Sentrin-specific protease 5
    EC=3.4.22.-
Alternative name(s):
    Sentrin/SUMO-specific protease SENP5

Gene names

Name:	SENP5
ORF Names:	FKSG45

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	755 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division. Ref.4 Ref.5
Subcellular location	Nucleus › nucleolus. Ref.4 Ref.5
Sequence similarities	Belongs to the peptidase C48 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 755	755	Sentrin-specific protease 5		PRO_0000101723
Regions
	Region	567 – 724	158	Protease
Sites
	Active site	646	1	By similarity
	Active site	663	1	By similarity
	Active site	713	1	Probable
Natural variations
	Natural variant	83	1	N → S: dbSNP rs35434690.		VAR_057045
Experimental info
	Mutagenesis	713	1	C → A: Abolishes enzymatic activity. Ref.4 Ref.5
	Sequence conflict	538	1	S → C in AAK69630. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q96HI0-1 [UniParc]. Last modified November 28, 2002. Version 2. Checksum: 1B89228FB56B3B29	FASTA	755	86,733
	10 20 30 40 50 60 MKKQRKILWR KGIHLAFSEK WNTGFGGFKK FYFHQHLCIL KAKLGRPVTW NRQLRHFQGR 70 80 90 100 110 120 KKALQIQKTW IKDEHLCAKT KFNVATQNVS TLSSKVKRKD AKHFISSSKT LLRLQAEKLL 130 140 150 160 170 180 SSAKNSDHEY CREKNLLKAV TDFPSNSALG QANGHRPRTD PQPSDFPMKF NGESQSPGES 190 200 210 220 230 240 GTIVVTLNNH KRKGFCYGCC QGPEHHRNGG PLIPKKFQLN QHRRIKLSPL MMYEKLSMIR 250 260 270 280 290 300 FRYRILRSQH FRTKSKVCKL RKAQRSWVQK VTGDHQETRR ENGEGGSCSP FPSPEPKDPS 310 320 330 340 350 360 CRHQPYFPDM DSSAVVKGTN SHVPDCHTKG SSFLGKELSL DEAFPDQQNG SATNAWDQSS 370 380 390 400 410 420 CSSPKWECTE LIHDIPLPEH RSNTMFISET EREIMTLGQE NQTSSVSDDR VKLSVSGADT 430 440 450 460 470 480 SVSSVDGPVS QKAVQNENSY QMEEDGSLKQ SILSSELLDH PYCKSPLEAP LVCSGLKLEN 490 500 510 520 530 540 QVGGGKNSQK ASPVDDEQLS VCLSGFLDEV MKKYGSLVPL SEKEVLGRLK DVFNEDFSNR 550 560 570 580 590 600 KPFINREITN YRARHQKCNF RIFYNKHMLD MDDLATLDGQ NWLNDQVINM YGELIMDAVP 610 620 630 640 650 660 DKVHFFNSFF HRQLVTKGYN GVKRWTKKVD LFKKSLLLIP IHLEVHWSLI TVTLSNRIIS 670 680 690 700 710 720 FYDSQGIHFK FCVENIRKYL LTEAREKNRP EFLQGWQTAV TKCIPQQKND SDCGVFVLQY 730 740 750 CKCLALEQPF QFSQEDMPRV RKRIYKELCE CRLMD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and Testis.
[2]	"Identification of FKSG45, a novel gene located on human chromosome 3." Wang Y.-G., Li T. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 305-755.
[3]	"Ubiquitin-like proteins: new wines in new bottles." Yeh E.T.H., Gong L., Kamitani T. Gene 248:1-14(2000) [PubMed: 10806345] [Abstract] Cited for: REVIEW.
[4]	"Characterization of a family of nucleolar SUMO-specific proteases with preference for SUMO-2 or SUMO-3." Gong L., Yeh E.T.H. J. Biol. Chem. 281:15869-15877(2006) [PubMed: 16608850] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-713.
[5]	"The SUMO-specific protease SENP5 is required for cell division." Di Bacco A., Ouyang J., Lee H.-Y., Catic A., Ploegh H., Gill G. Mol. Cell. Biol. 26:4489-4498(2006) [PubMed: 16738315] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-713.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC008589 mRNA. Translation: AAH08589.1. BC030705 mRNA. Translation: AAH30705.1. AF335474 mRNA. Translation: AAK69630.1. Different initiation.
IPI	IPI00063106.
RefSeq	NP_689912.2.
UniGene	Hs.240770
3D structure databases
HSSP	HSSP built from PDB template 1EUV based on UniProtKB Q02724.
ModBase	Search...
Protein family/group databases
MEROPS	C48.008.
PTM databases
PhosphoSite	Q96HI0.
Proteomic databases
PRIDE	Q96HI0.
Genome annotation databases
Ensembl	ENSG00000119231. Homo sapiens. [Contig view]
GeneID	205564.
KEGG	hsa:205564.
Organism-specific databases
GeneCards	GC03P198083.
H-InvDB	HIX0018042.
HGNC	HGNC:28407. SENP5.
PharmGKB	PA134917083.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96HI0.
HOVERGEN	Q96HI0.
Gene expression databases
ArrayExpress	Q96HI0.
Bgee	Q96HI0.
CleanEx	HS_SENP5.
GermOnline	ENSG00000119231. Homo sapiens.
Family and domain databases
InterPro	IPR003653. Peptidase_C48. [Graphical view]
Pfam	PF02902. Peptidase_C48. 1 hit. [Graphical view]
PROSITE	PS50600. ULP_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	90512.

Entry information

Entry name

SENP5_HUMAN

Accession

Primary (citable) accession number: Q96HI0
Secondary accession number(s): Q96SA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	November 28, 2002
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)