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Protein

Sentrin-specific protease 5

Gene

SENP5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division.2 Publications

Catalytic activityi

Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei646 – 6461By similarity
Active sitei663 – 6631By similarity
Active sitei713 – 7131Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Ubl conjugation pathway

Enzyme and pathway databases

BRENDAi3.4.22.B73. 2681.
ReactomeiREACT_163725. SUMO is proteolytically processed.

Protein family/group databases

MEROPSiC48.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Sentrin-specific protease 5 (EC:3.4.22.68)
Alternative name(s):
Sentrin/SUMO-specific protease SENP5
Gene namesi
Name:SENP5
ORF Names:FKSG45
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:28407. SENP5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi713 – 7131C → A: Abolishes enzymatic activity. 2 Publications

Organism-specific databases

PharmGKBiPA134917083.

Polymorphism and mutation databases

BioMutaiSENP5.
DMDMi296452962.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 755755Sentrin-specific protease 5PRO_0000101723Add
BLAST

Proteomic databases

MaxQBiQ96HI0.
PaxDbiQ96HI0.
PRIDEiQ96HI0.

PTM databases

PhosphoSiteiQ96HI0.

Expressioni

Gene expression databases

BgeeiQ96HI0.
CleanExiHS_SENP5.
ExpressionAtlasiQ96HI0. baseline and differential.
GenevisibleiQ96HI0. HS.

Interactioni

Subunit structurei

Interacts with CCAR2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ESR1P033722EBI-3895753,EBI-78473

Protein-protein interaction databases

BioGridi128497. 13 interactions.
IntActiQ96HI0. 1 interaction.
STRINGi9606.ENSP00000327197.

Structurei

3D structure databases

ProteinModelPortaliQ96HI0.
SMRiQ96HI0. Positions 515-754.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni567 – 724158ProteaseAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

eggNOGiCOG5160.
GeneTreeiENSGT00530000062941.
HOGENOMiHOG000070166.
HOVERGENiHBG062231.
InParanoidiQ96HI0.
KOiK08594.
OMAiAFPDQQN.
OrthoDBiEOG7D59MP.
PhylomeDBiQ96HI0.
TreeFamiTF316289.

Family and domain databases

InterProiIPR003653. Peptidase_C48.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96HI0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKQRKILWR KGIHLAFSEK WNTGFGGFKK FYFHQHLCIL KAKLGRPVTW
60 70 80 90 100
NRQLRHFQGR KKALQIQKTW IKDEPLCAKT KFNVATQNVS TLSSKVKRKD
110 120 130 140 150
AKHFISSSKT LLRLQAEKLL SSAKNSDHEY CREKNLLKAV TDFPSNSALG
160 170 180 190 200
QANGHRPRTD PQPSDFPMKF NGESQSPGES GTIVVTLNNH KRKGFCYGCC
210 220 230 240 250
QGPEHHRNGG PLIPKKFQLN QHRRIKLSPL MMYEKLSMIR FRYRILRSQH
260 270 280 290 300
FRTKSKVCKL RKAQRSWVQK VTGDHQETRR ENGEGGSCSP FPSPEPKDPS
310 320 330 340 350
CRHQPYFPDM DSSAVVKGTN SHVPDCHTKG SSFLGKELSL DEAFPDQQNG
360 370 380 390 400
SATNAWDQSS CSSPKWECTE LIHDIPLPEH RSNTMFISET EREIMTLGQE
410 420 430 440 450
NQTSSVSDDR VKLSVSGADT SVSSVDGPVS QKAVQNENSY QMEEDGSLKQ
460 470 480 490 500
SILSSELLDH PYCKSPLEAP LVCSGLKLEN QVGGGKNSQK ASPVDDEQLS
510 520 530 540 550
VCLSGFLDEV MKKYGSLVPL SEKEVLGRLK DVFNEDFSNR KPFINREITN
560 570 580 590 600
YRARHQKCNF RIFYNKHMLD MDDLATLDGQ NWLNDQVINM YGELIMDAVP
610 620 630 640 650
DKVHFFNSFF HRQLVTKGYN GVKRWTKKVD LFKKSLLLIP IHLEVHWSLI
660 670 680 690 700
TVTLSNRIIS FYDSQGIHFK FCVENIRKYL LTEAREKNRP EFLQGWQTAV
710 720 730 740 750
TKCIPQQKND SDCGVFVLQY CKCLALEQPF QFSQEDMPRV RKRIYKELCE

CRLMD
Length:755
Mass (Da):86,693
Last modified:May 18, 2010 - v3
Checksum:i90D7B34B2853CEE4
GO
Isoform 2 (identifier: Q96HI0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     629-674: Missing.

Note: No experimental confirmation available.
Show »
Length:709
Mass (Da):81,310
Checksum:iBA608D50017296EC
GO

Sequence cautioni

The sequence AAK69630.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751P → H in AAH30705 (PubMed:15489334).Curated
Sequence conflicti538 – 5381S → C in AAK69630 (Ref. 4) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti83 – 831N → S.
Corresponds to variant rs35434690 [ dbSNP | Ensembl ].
VAR_057045
Natural varianti340 – 3401L → F.
Corresponds to variant rs34251880 [ dbSNP | Ensembl ].
VAR_061732

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei629 – 67446Missing in isoform 2. 1 PublicationVSP_056415Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK302305 mRNA. Translation: BAG63644.1.
AC011322 Genomic DNA. No translation available.
AC016949 Genomic DNA. No translation available.
AC127904 Genomic DNA. No translation available.
BC008589 mRNA. Translation: AAH08589.1.
BC030705 mRNA. Translation: AAH30705.1.
AF335474 mRNA. Translation: AAK69630.1. Different initiation.
CCDSiCCDS3322.1. [Q96HI0-1]
RefSeqiNP_689912.2. NM_152699.4. [Q96HI0-1]
UniGeneiHs.240770.

Genome annotation databases

EnsembliENST00000323460; ENSP00000327197; ENSG00000119231. [Q96HI0-1]
ENST00000445299; ENSP00000390231; ENSG00000119231. [Q96HI0-2]
GeneIDi205564.
KEGGihsa:205564.
UCSCiuc003fwz.4. human. [Q96HI0-1]
uc011bty.2. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK302305 mRNA. Translation: BAG63644.1.
AC011322 Genomic DNA. No translation available.
AC016949 Genomic DNA. No translation available.
AC127904 Genomic DNA. No translation available.
BC008589 mRNA. Translation: AAH08589.1.
BC030705 mRNA. Translation: AAH30705.1.
AF335474 mRNA. Translation: AAK69630.1. Different initiation.
CCDSiCCDS3322.1. [Q96HI0-1]
RefSeqiNP_689912.2. NM_152699.4. [Q96HI0-1]
UniGeneiHs.240770.

3D structure databases

ProteinModelPortaliQ96HI0.
SMRiQ96HI0. Positions 515-754.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128497. 13 interactions.
IntActiQ96HI0. 1 interaction.
STRINGi9606.ENSP00000327197.

Protein family/group databases

MEROPSiC48.008.

PTM databases

PhosphoSiteiQ96HI0.

Polymorphism and mutation databases

BioMutaiSENP5.
DMDMi296452962.

Proteomic databases

MaxQBiQ96HI0.
PaxDbiQ96HI0.
PRIDEiQ96HI0.

Protocols and materials databases

DNASUi205564.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000323460; ENSP00000327197; ENSG00000119231. [Q96HI0-1]
ENST00000445299; ENSP00000390231; ENSG00000119231. [Q96HI0-2]
GeneIDi205564.
KEGGihsa:205564.
UCSCiuc003fwz.4. human. [Q96HI0-1]
uc011bty.2. human.

Organism-specific databases

CTDi205564.
GeneCardsiGC03P196594.
H-InvDBHIX0018042.
HGNCiHGNC:28407. SENP5.
MIMi612845. gene.
neXtProtiNX_Q96HI0.
PharmGKBiPA134917083.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5160.
GeneTreeiENSGT00530000062941.
HOGENOMiHOG000070166.
HOVERGENiHBG062231.
InParanoidiQ96HI0.
KOiK08594.
OMAiAFPDQQN.
OrthoDBiEOG7D59MP.
PhylomeDBiQ96HI0.
TreeFamiTF316289.

Enzyme and pathway databases

BRENDAi3.4.22.B73. 2681.
ReactomeiREACT_163725. SUMO is proteolytically processed.

Miscellaneous databases

ChiTaRSiSENP5. human.
GenomeRNAii205564.
NextBioi35476153.
PROiQ96HI0.
SOURCEiSearch...

Gene expression databases

BgeeiQ96HI0.
CleanExiHS_SENP5.
ExpressionAtlasiQ96HI0. baseline and differential.
GenevisibleiQ96HI0. HS.

Family and domain databases

InterProiIPR003653. Peptidase_C48.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  2. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta and Testis.
  4. "Identification of FKSG45, a novel gene located on human chromosome 3."
    Wang Y.-G., Li T.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 305-755 (ISOFORM 1).
  5. "Ubiquitin-like proteins: new wines in new bottles."
    Yeh E.T.H., Gong L., Kamitani T.
    Gene 248:1-14(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  6. "Characterization of a family of nucleolar SUMO-specific proteases with preference for SUMO-2 or SUMO-3."
    Gong L., Yeh E.T.H.
    J. Biol. Chem. 281:15869-15877(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-713.
  7. "The SUMO-specific protease SENP5 is required for cell division."
    Di Bacco A., Ouyang J., Lee H.-Y., Catic A., Ploegh H., Gill G.
    Mol. Cell. Biol. 26:4489-4498(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-713.
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  9. "Modification of DBC1 by SUMO2/3 is crucial for p53-mediated apoptosis in response to DNA damage."
    Park J.H., Lee S.W., Yang S.W., Yoo H.M., Park J.M., Seong M.W., Ka S.H., Oh K.H., Jeon Y.J., Chung C.H.
    Nat. Commun. 5:5483-5483(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCAR2.

Entry informationi

Entry nameiSENP5_HUMAN
AccessioniPrimary (citable) accession number: Q96HI0
Secondary accession number(s): B4DY82, Q96SA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: May 18, 2010
Last modified: June 24, 2015
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.