Reviewed, UniProtKB/Swiss-Prot Q96HF1 (SFRP2_HUMAN)

Names and origin

Protein names

Recommended name:
    Secreted frizzled-related protein 2
      Short name=sFRP-2
Alternative name(s):
    Secreted apoptosis-related protein 1
      Short name=SARP-1
    Frizzled-related protein 2
      Short name=FRP-2

Gene names

Name:	SFRP2
Synonyms:	FRP2, SARP1
ORF Names:	FKSG12, UNQ361/PRO697

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. Ref.1 Ref.7
Domain	The FZ domain is involved in binding with Wnt ligands By similarity.
Sequence similarities	Belongs to the secreted frizzled-related protein (sFRP) family. Contains 1 FZ (frizzled) domain. Contains 1 NTR domain.

Ontologies

Keywords
Biological process	Differentiation Wnt signaling pathway
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Developmental protein
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.8
	Chain	25 – 295	271	Secreted frizzled-related protein 2		PRO_0000032542
Regions
	Domain	35 – 155	121	FZ
	Domain	172 – 295	124	NTR
Amino acid modifications
	Modified residue	289	1	Phosphoserine Ref.9
	Disulfide bond	40 ↔ 103		By similarity
	Disulfide bond	50 ↔ 96		By similarity
	Disulfide bond	87 ↔ 125		By similarity
	Disulfide bond	114 ↔ 152		By similarity
	Disulfide bond	118 ↔ 142		By similarity
	Disulfide bond	172 ↔ 245		By similarity
	Disulfide bond	175 ↔ 247		By similarity
	Disulfide bond	190 ↔ 295		By similarity
Natural variations
	Natural variant	45	1	A → V: dbSNP rs4643790. Ref.3 Ref.6		VAR_051963
Experimental info
	Sequence conflict	132 – 133	2	Missing in AAB70792. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q96HF1-1 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 97597971541BFBC4	FASTA	295	33,490
	10 20 30 40 50 60 MLQGPGSLLL LFLASHCCLG SARGLFLFGQ PDFSYKRSNC KPIPANLQLC HGIEYQNMRL 70 80 90 100 110 120 PNLLGHETMK EVLEQAGAWI PLVMKQCHPD TKKFLCSLFA PVCLDDLDET IQPCHSLCVQ 130 140 150 160 170 180 VKDRCAPVMS AFGFPWPDML ECDRFPQDND LCIPLASSDH LLPATEEAPK VCEACKNKND 190 200 210 220 230 240 DDNDIMETLC KNDFALKIKV KEITYINRDT KIILETKSKT IYKLNGVSER DLKKSVLWLK 250 260 270 280 290 DSLQCTCEEM NDINAPYLVM GQKQGGELVI TSVKRWQKGQ REFKRISRSI RKLQC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tissue restricted expression of two human Frzbs in preadipocytes and pancreas." Hu E., Zhu Y., Fredrickson T., Barnes M., Kelsell D., Beeley L., Brooks D. Biochem. Biophys. Res. Commun. 247:287-293(1998) [PubMed: 9642118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Characterization of FKSG12, a novel protein related to pancreas tumor." Wang Y.-G. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-45.
[4]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed: 16303743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-45. Tissue: Eye.
[7]	"SARPs: a family of secreted apoptosis-related proteins." Melkonyan H.S., Chang W.C., Shapiro J.P., Mahadevappa M., Fitzpatrick P.A., Kiefer M.C., Tomei L.D., Umansky S.R. Proc. Natl. Acad. Sci. U.S.A. 94:13636-13641(1997) [PubMed: 9391078] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-208, TISSUE SPECIFICITY.
[8]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-39.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289, MASS SPECTROMETRY. Tissue: Epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF311912 mRNA. Translation: AAG24923.1. AY359001 mRNA. Translation: AAQ89360.1. AK075372 mRNA. Translation: BAG52124.1. CH471056 Genomic DNA. Translation: EAX04949.1. BC008666 mRNA. Translation: AAH08666.1. AF017986 mRNA. Translation: AAB70792.1.
IPI	IPI00027596.
PIR	JE0174.
RefSeq	NP_003004.1.
UniGene	Hs.481022
3D structure databases
SMR	Q96HF1. Positions 39-154.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96HF1.
Proteomic databases
PRIDE	Q96HF1.
Genome annotation databases
Ensembl	ENST00000274063; ENSP00000274063; ENSG00000145423; Homo sapiens. [Genome view]
GeneID	6423.
KEGG	hsa:6423.
UCSC	uc003inv.1. human.
Organism-specific databases
CTD	6423.
GeneCards	GC04M154921.
H-InvDB	HIX0004583.
HGNC	HGNC:10777. SFRP2.
HPA	HPA002652.
MIM	604157. gene.
PharmGKB	PA35693.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG08093.
HOGENOM	HBG713519.
HOVERGEN	Q96HF1.
InParanoid	Q96HF1.
OMA	CKNKNED.
OrthoDB	EOG9WWV46.
PhylomeDB	Q96HF1.
Gene expression databases
ArrayExpress	Q96HF1.
Bgee	Q96HF1.
CleanEx	HS_SFRP2.
Genevestigator	Q96HF1.
GermOnline	ENSG00000145423. Homo sapiens.
Family and domain databases
InterPro	IPR020067. Frizzled-like_dom. IPR000024. Frizzled_Cys-rich. IPR020068. Frizzled_dom_subgr. IPR015526. Frizzled_related. IPR001134. Netrin_domain. IPR018933. Netrin_module_non-TIMP. IPR008993. TIMP-like_OB-fold. [Graphical view]
Gene3D	G3DSA:1.10.2000.10. Frizzled_Cys-rich. 1 hit.
PANTHER	PTHR11309. Fz_related. 1 hit.
Pfam	PF01392. Fz. 1 hit. PF01759. NTR. 1 hit. [Graphical view]
SMART	SM00643. C345C. 1 hit. SM00063. FRI. 1 hit. [Graphical view]
PROSITE	PS50038. FZ. 1 hit. PS50189. NTR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	24943.
SOURCE	Search...

Entry information

Entry name

SFRP2_HUMAN

Accession

Primary (citable) accession number: Q96HF1
Secondary accession number(s): B3KQR2, O14778, Q9HAP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 19, 2010
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.