Q96HF1 (SFRP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Secreted frizzled-related protein 2
Short name=FRP-2
Short name=sFRP-2

Alternative name(s):
Secreted apoptosis-related protein 1
Short name=SARP-1

Gene names

Name:	SFRP2
Synonyms:	FRP2, SARP1
ORF Names:	FKSG12, UNQ361/PRO697

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. Ref.1 Ref.7
Domain	The FZ domain is involved in binding with Wnt ligands By similarity.
Sequence similarities	Belongs to the secreted frizzled-related protein (sFRP) family. Contains 1 FZ (frizzled) domain. Contains 1 NTR domain.

Ontologies

Keywords
Biological process	Differentiation Wnt signaling pathway
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Developmental protein
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway involved in somitogenesis Inferred from electronic annotation. Source: Compara bone morphogenesis Inferred from electronic annotation. Source: Compara brain development Inferred from Biological aspect of Ancestor. Source: RefGenome cardiac left ventricle morphogenesis Inferred from mutant phenotype PubMed 19593212. Source: BHF-UCL cell-cell signaling Inferred from sequence or structural similarity. Source: BHF-UCL cellular response to X-ray Inferred from electronic annotation. Source: Compara cellular response to extracellular stimulus Inferred from electronic annotation. Source: Compara chondrocyte development Inferred from electronic annotation. Source: Compara collagen fibril organization Inferred from electronic annotation. Source: Compara convergent extension involved in axis elongation Inferred from electronic annotation. Source: Compara digestive tract morphogenesis Inferred from electronic annotation. Source: Compara embryonic digit morphogenesis Inferred from electronic annotation. Source: Compara gonad development Inferred from Biological aspect of Ancestor. Source: RefGenome hematopoietic stem cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB male gonad development Inferred from electronic annotation. Source: Compara negative regulation of BMP signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of JUN kinase activity Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 17471511. Source: UniProtKB negative regulation of cardiac muscle cell apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of cell growth Inferred from direct assay PubMed 20208569. Source: UniProtKB negative regulation of cell migration Inferred from sequence or structural similarity PubMed 10980594. Source: UniProtKB negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of dermatome development Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of epithelial cell proliferation Inferred from direct assay PubMed 19095296. Source: UniProtKB negative regulation of epithelial to mesenchymal transition Inferred from direct assay PubMed 19095296. Source: UniProtKB negative regulation of mesodermal cell fate specification Inferred from electronic annotation. Source: Compara negative regulation of peptidyl-tyrosine phosphorylation Inferred from sequence or structural similarity PubMed 10980594. Source: UniProtKB negative regulation of planar cell polarity pathway involved in axis elongation Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from direct assay PubMed 19095296. Source: UniProtKB neural tube closure Inferred from electronic annotation. Source: Compara outflow tract morphogenesis Inferred from mutant phenotype PubMed 19593212. Source: BHF-UCL patterning of blood vessels Inferred from sequence or structural similarity. Source: BHF-UCL planar cell polarity pathway involved in neural tube closure Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of apoptotic process Inferred from mutant phenotype PubMed 17848950. Source: BHF-UCL positive regulation of canonical Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of catenin import into nucleus Inferred from electronic annotation. Source: Compara positive regulation of cell adhesion mediated by integrin Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of cell growth Inferred from sequence or structural similarity PubMed 10980594. Source: UniProtKB positive regulation of cell proliferation Inferred from direct assay PubMed 10980594. Source: UniProtKB positive regulation of fat cell differentiation Inferred from direct assay PubMed 12055200. Source: MGI positive regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-serine phosphorylation Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: BHF-UCL post-anal tail morphogenesis Inferred from electronic annotation. Source: Compara regulation of stem cell division Inferred from sequence or structural similarity. Source: UniProtKB response to drug Inferred from electronic annotation. Source: Compara sclerotome development Inferred from sequence or structural similarity. Source: BHF-UCL
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular matrix Inferred from sequence or structural similarity. Source: BHF-UCL extracellular space Inferred from direct assay PubMed 10980594. Source: UniProtKB plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	PDZ domain binding Inferred from Biological aspect of Ancestor. Source: RefGenome Wnt-activated receptor activity Inferred from Biological aspect of Ancestor. Source: RefGenome Wnt-protein binding Inferred from Biological aspect of Ancestor. Source: RefGenome endopeptidase activator activity Inferred from electronic annotation. Source: Compara fibronectin binding Inferred from sequence or structural similarity. Source: BHF-UCL integrin binding Inferred from sequence or structural similarity. Source: BHF-UCL metalloenzyme activator activity Inferred from electronic annotation. Source: Compara receptor agonist activity Inferred from sequence or structural similarity. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.8
	Chain	25 – 295	271	Secreted frizzled-related protein 2		PRO_0000032542
Regions
	Domain	35 – 155	121	FZ
	Domain	172 – 295	124	NTR
Amino acid modifications
	Disulfide bond	40 ↔ 103		By similarity
	Disulfide bond	50 ↔ 96		By similarity
	Disulfide bond	87 ↔ 125		By similarity
	Disulfide bond	114 ↔ 152		By similarity
	Disulfide bond	118 ↔ 142		By similarity
	Disulfide bond	172 ↔ 245		By similarity
	Disulfide bond	175 ↔ 247		By similarity
	Disulfide bond	190 ↔ 295		By similarity
Natural variations
	Natural variant	45	1	A → V. Ref.3 Ref.6 Corresponds to variant rs4643790 [ dbSNP | Ensembl ].		VAR_051963
Experimental info
	Sequence conflict	132 – 133	2	Missing in AAB70792. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q96HF1 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 97597971541BFBC4	FASTA	295	33,490
	10 20 30 40 50 60 MLQGPGSLLL LFLASHCCLG SARGLFLFGQ PDFSYKRSNC KPIPANLQLC HGIEYQNMRL 70 80 90 100 110 120 PNLLGHETMK EVLEQAGAWI PLVMKQCHPD TKKFLCSLFA PVCLDDLDET IQPCHSLCVQ 130 140 150 160 170 180 VKDRCAPVMS AFGFPWPDML ECDRFPQDND LCIPLASSDH LLPATEEAPK VCEACKNKND 190 200 210 220 230 240 DDNDIMETLC KNDFALKIKV KEITYINRDT KIILETKSKT IYKLNGVSER DLKKSVLWLK 250 260 270 280 290 DSLQCTCEEM NDINAPYLVM GQKQGGELVI TSVKRWQKGQ REFKRISRSI RKLQC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tissue restricted expression of two human Frzbs in preadipocytes and pancreas." Hu E., Zhu Y., Fredrickson T., Barnes M., Kelsell D., Beeley L., Brooks D. Biochem. Biophys. Res. Commun. 247:287-293(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Characterization of FKSG12, a novel protein related to pancreas tumor." Wang Y.-G. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-45.
[4]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-45. Tissue: Eye.
[7]	"SARPs: a family of secreted apoptosis-related proteins." Melkonyan H.S., Chang W.C., Shapiro J.P., Mahadevappa M., Fitzpatrick P.A., Kiefer M.C., Tomei L.D., Umansky S.R. Proc. Natl. Acad. Sci. U.S.A. 94:13636-13641(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-208, TISSUE SPECIFICITY.
[8]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-39.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF311912 mRNA. Translation: AAG24923.1. AY359001 mRNA. Translation: AAQ89360.1. AK075372 mRNA. Translation: BAG52124.1. CH471056 Genomic DNA. Translation: EAX04949.1. BC008666 mRNA. Translation: AAH08666.1. AF017986 mRNA. Translation: AAB70792.1.
IPI	IPI00027596.
PIR	JE0174.
RefSeq	NP_003004.1. NM_003013.2.
UniGene	Hs.481022.
3D structure databases
ProteinModelPortal	Q96HF1.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000274063.
PTM databases
PhosphoSite	Q96HF1.
Polymorphism databases
DMDM	61216828.
Proteomic databases
PaxDb	Q96HF1.
PRIDE	Q96HF1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000274063; ENSP00000274063; ENSG00000145423.
GeneID	6423.
KEGG	hsa:6423.
UCSC	uc003inv.1. human.
Organism-specific databases
CTD	6423.
GeneCards	GC04M154701.
HGNC	HGNC:10777. SFRP2.
HPA	HPA002652.
MIM	604157. gene.
neXtProt	NX_Q96HF1.
PharmGKB	PA35693.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG240950.
HOGENOM	HOG000231058.
HOVERGEN	HBG052928.
InParanoid	Q96HF1.
KO	K02176.
OMA	CKNKNED.
OrthoDB	EOG48GW3T.
PhylomeDB	Q96HF1.
Gene expression databases
ArrayExpress	Q96HF1.
Bgee	Q96HF1.
CleanEx	HS_SFRP2.
Genevestigator	Q96HF1.
GermOnline	ENSG00000145423. Homo sapiens.
Family and domain databases
Gene3D	1.10.2000.10. 1 hit.
InterPro	IPR015526. Frizzled/SFRP. IPR020067. Frizzled_dom. IPR001134. Netrin_domain. IPR018933. Netrin_module_non-TIMP. IPR026558. SFRP2. IPR008993. TIMP-like_OB-fold. [Graphical view]
PANTHER	PTHR11309. PTHR11309. 1 hit. PTHR11309:SF45. PTHR11309:SF45. 1 hit.
Pfam	PF01392. Fz. 1 hit. PF01759. NTR. 1 hit. [Graphical view]
SMART	SM00643. C345C. 1 hit. SM00063. FRI. 1 hit. [Graphical view]
SUPFAM	SSF63501. Frizzled_Cys-rich. 1 hit. SSF50242. TIMP_like. 1 hit.
PROSITE	PS50038. FZ. 1 hit. PS50189. NTR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6423.
NextBio	24943.
SOURCE	Search...

Entry information

Entry name

SFRP2_HUMAN

Accession

Primary (citable) accession number: Q96HF1
Secondary accession number(s): B3KQR2, O14778, Q9HAP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.