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Q96HF1 (SFRP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Secreted frizzled-related protein 2

Short name=FRP-2
Short name=sFRP-2
Alternative name(s):
Secreted apoptosis-related protein 1
Short name=SARP-1
Gene names
Name:SFRP2
Synonyms:FRP2, SARP1
ORF Names:FKSG12, UNQ361/PRO697
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length295 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. Ref.1 Ref.7

Domain

The FZ domain is involved in binding with Wnt ligands By similarity.

Sequence similarities

Belongs to the secreted frizzled-related protein (sFRP) family.

Contains 1 FZ (frizzled) domain.

Contains 1 NTR domain.

Ontologies

Keywords
   Biological processDifferentiation
Wnt signaling pathway
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionDevelopmental protein
   PTMDisulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway involved in somitogenesis

Inferred from electronic annotation. Source: Ensembl

bone morphogenesis

Inferred from electronic annotation. Source: Ensembl

brain development

Inferred from Biological aspect of Ancestor. Source: RefGenome

cardiac left ventricle morphogenesis

Inferred from mutant phenotype PubMed 19593212. Source: BHF-UCL

cell-cell signaling

Inferred from sequence or structural similarity. Source: BHF-UCL

cellular response to X-ray

Inferred from electronic annotation. Source: Ensembl

cellular response to extracellular stimulus

Inferred from electronic annotation. Source: Ensembl

chondrocyte development

Inferred from electronic annotation. Source: Ensembl

collagen fibril organization

Inferred from electronic annotation. Source: Ensembl

convergent extension involved in axis elongation

Inferred from electronic annotation. Source: Ensembl

digestive tract morphogenesis

Inferred from electronic annotation. Source: Ensembl

embryo development

Inferred from Biological aspect of Ancestor. Source: RefGenome

embryonic digit morphogenesis

Inferred from electronic annotation. Source: Ensembl

gonad development

Inferred from Biological aspect of Ancestor. Source: RefGenome

hematopoietic stem cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

male gonad development

Inferred from electronic annotation. Source: Ensembl

negative regulation of BMP signaling pathway

Inferred from electronic annotation. Source: Ensembl

negative regulation of JUN kinase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of Wnt signaling pathway

Inferred from direct assay PubMed 20208569. Source: UniProtKB

negative regulation of canonical Wnt signaling pathway

Inferred from direct assay PubMed 17471511. Source: UniProtKB

negative regulation of cardiac muscle cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell growth

Inferred from direct assay PubMed 20208569. Source: UniProtKB

negative regulation of cell migration

Inferred from sequence or structural similarity PubMed 10980594. Source: UniProtKB

negative regulation of cell proliferation

Inferred from direct assay PubMed 20208569. Source: UniProtKB

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of dermatome development

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of epithelial cell proliferation

Inferred from direct assay PubMed 19095296. Source: UniProtKB

negative regulation of epithelial to mesenchymal transition

Inferred from direct assay PubMed 19095296. Source: UniProtKB

negative regulation of extrinsic apoptotic signaling pathway via death domain receptors

Inferred from electronic annotation. Source: Ensembl

negative regulation of gene expression

Inferred from sequence or structural similarity PubMed 10980594. Source: UniProtKB

negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of mesodermal cell fate specification

Inferred from electronic annotation. Source: Ensembl

negative regulation of peptidyl-tyrosine phosphorylation

Inferred from sequence or structural similarity PubMed 10980594. Source: UniProtKB

negative regulation of planar cell polarity pathway involved in axis elongation

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 19095296. Source: UniProtKB

neural tube closure

Inferred from electronic annotation. Source: Ensembl

outflow tract morphogenesis

Inferred from mutant phenotype PubMed 19593212. Source: BHF-UCL

patterning of blood vessels

Inferred from sequence or structural similarity. Source: BHF-UCL

planar cell polarity pathway involved in neural tube closure

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of apoptotic process

Inferred from mutant phenotype PubMed 17848950. Source: BHF-UCL

positive regulation of canonical Wnt signaling pathway

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of catenin import into nucleus

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell adhesion mediated by integrin

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of cell growth

Inferred from sequence or structural similarity PubMed 10980594. Source: UniProtKB

positive regulation of cell proliferation

Inferred from direct assay PubMed 10980594. Source: UniProtKB

positive regulation of fat cell differentiation

Inferred from direct assay PubMed 12055200. Source: MGI

positive regulation of osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-serine phosphorylation

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: BHF-UCL

post-anal tail morphogenesis

Inferred from electronic annotation. Source: Ensembl

regulation of stem cell division

Inferred from sequence or structural similarity. Source: UniProtKB

response to drug

Inferred from electronic annotation. Source: Ensembl

response to nutrient

Inferred from electronic annotation. Source: Ensembl

sclerotome development

Inferred from sequence or structural similarity. Source: BHF-UCL

vasculature development

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentcytoplasm

Inferred from Biological aspect of Ancestor. Source: RefGenome

extracellular matrix

Inferred from sequence or structural similarity. Source: BHF-UCL

extracellular space

Inferred from direct assay PubMed 10980594. Source: UniProtKB

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionPDZ domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-activated receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-protein binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

endopeptidase activator activity

Inferred from electronic annotation. Source: Ensembl

fibronectin binding

Inferred from sequence or structural similarity. Source: BHF-UCL

integrin binding

Inferred from sequence or structural similarity. Source: BHF-UCL

metalloenzyme activator activity

Inferred from electronic annotation. Source: Ensembl

receptor agonist activity

Inferred from sequence or structural similarity. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.8
Chain25 – 295271Secreted frizzled-related protein 2
PRO_0000032542

Regions

Domain35 – 155121FZ
Domain172 – 295124NTR

Amino acid modifications

Disulfide bond40 ↔ 103 By similarity
Disulfide bond50 ↔ 96 By similarity
Disulfide bond87 ↔ 125 By similarity
Disulfide bond114 ↔ 152 By similarity
Disulfide bond118 ↔ 142 By similarity
Disulfide bond172 ↔ 245 By similarity
Disulfide bond175 ↔ 247 By similarity
Disulfide bond190 ↔ 295 By similarity

Natural variations

Natural variant451A → V. Ref.3 Ref.6
Corresponds to variant rs4643790 [ dbSNP | Ensembl ].
VAR_051963

Experimental info

Sequence conflict132 – 1332Missing in AAB70792. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q96HF1 [UniParc].

Last modified March 15, 2005. Version 2.
Checksum: 97597971541BFBC4

FASTA29533,490
        10         20         30         40         50         60 
MLQGPGSLLL LFLASHCCLG SARGLFLFGQ PDFSYKRSNC KPIPANLQLC HGIEYQNMRL 

        70         80         90        100        110        120 
PNLLGHETMK EVLEQAGAWI PLVMKQCHPD TKKFLCSLFA PVCLDDLDET IQPCHSLCVQ 

       130        140        150        160        170        180 
VKDRCAPVMS AFGFPWPDML ECDRFPQDND LCIPLASSDH LLPATEEAPK VCEACKNKND 

       190        200        210        220        230        240 
DDNDIMETLC KNDFALKIKV KEITYINRDT KIILETKSKT IYKLNGVSER DLKKSVLWLK 

       250        260        270        280        290 
DSLQCTCEEM NDINAPYLVM GQKQGGELVI TSVKRWQKGQ REFKRISRSI RKLQC 

« Hide

References

« Hide 'large scale' references
[1]"Tissue restricted expression of two human Frzbs in preadipocytes and pancreas."
Hu E., Zhu Y., Fredrickson T., Barnes M., Kelsell D., Beeley L., Brooks D.
Biochem. Biophys. Res. Commun. 247:287-293(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"Characterization of FKSG12, a novel protein related to pancreas tumor."
Wang Y.-G.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-45.
[4]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-45.
Tissue: Eye.
[7]"SARPs: a family of secreted apoptosis-related proteins."
Melkonyan H.S., Chang W.C., Shapiro J.P., Mahadevappa M., Fitzpatrick P.A., Kiefer M.C., Tomei L.D., Umansky S.R.
Proc. Natl. Acad. Sci. U.S.A. 94:13636-13641(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-208, TISSUE SPECIFICITY.
[8]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-39.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF311912 mRNA. Translation: AAG24923.1.
AY359001 mRNA. Translation: AAQ89360.1.
AK075372 mRNA. Translation: BAG52124.1.
CH471056 Genomic DNA. Translation: EAX04949.1.
BC008666 mRNA. Translation: AAH08666.1.
AF017986 mRNA. Translation: AAB70792.1.
PIRJE0174.
RefSeqNP_003004.1. NM_003013.2.
UniGeneHs.481022.

3D structure databases

ProteinModelPortalQ96HF1.
SMRQ96HF1. Positions 49-150.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112321. 1 interaction.
STRING9606.ENSP00000274063.

Protein family/group databases

MEROPSI93.001.

PTM databases

PhosphoSiteQ96HF1.

Polymorphism databases

DMDM61216828.

Proteomic databases

PaxDbQ96HF1.
PRIDEQ96HF1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000274063; ENSP00000274063; ENSG00000145423.
GeneID6423.
KEGGhsa:6423.
UCSCuc003inv.1. human.

Organism-specific databases

CTD6423.
GeneCardsGC04M154701.
HGNCHGNC:10777. SFRP2.
HPAHPA002652.
MIM604157. gene.
neXtProtNX_Q96HF1.
PharmGKBPA35693.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG240950.
HOGENOMHOG000231058.
HOVERGENHBG052928.
InParanoidQ96HF1.
KOK02176.
OMAGHETMNE.
OrthoDBEOG789CBZ.
PhylomeDBQ96HF1.
TreeFamTF350133.

Enzyme and pathway databases

SignaLinkQ96HF1.

Gene expression databases

ArrayExpressQ96HF1.
BgeeQ96HF1.
CleanExHS_SFRP2.
GenevestigatorQ96HF1.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR026558. SFRP2.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF45. PTHR11309:SF45. 1 hit.
PfamPF01392. Fz. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTSM00643. C345C. 1 hit.
SM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF50242. SSF50242. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSFRP2.
GenomeRNAi6423.
NextBio24943.
PROQ96HF1.
SOURCESearch...

Entry information

Entry nameSFRP2_HUMAN
AccessionPrimary (citable) accession number: Q96HF1
Secondary accession number(s): B3KQR2, O14778, Q9HAP5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: April 16, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM