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Protein

N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)

Gene

ACY3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids (By similarity).By similarity

Catalytic activityi

An N-acyl-aromatic-L-amino acid + H2O = an aromatic-L-amino acid + a carboxylate.
An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi21 – 211ZincBy similarity
Metal bindingi24 – 241ZincBy similarity
Binding sitei63 – 631SubstrateBy similarity
Metal bindingi116 – 1161ZincBy similarity
Binding sitei178 – 1781SubstrateBy similarity
Binding sitei288 – 2881SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:HS13441-MONOMER.
ReactomeiREACT_264461. Aflatoxin activation and detoxification.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming) (EC:3.5.1.114)
Alternative name(s):
Acylase III
Aminoacylase-3
Short name:
ACY-3
Aspartoacylase-2
Hepatitis C virus core-binding protein 1
Short name:
HCBP1
Short name:
HCV core-binding protein 1
Gene namesi
Name:ACY3
Synonyms:ASPA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:24104. ACY3.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134936640.

Chemistry

DrugBankiDB00128. L-Aspartic Acid.

Polymorphism and mutation databases

BioMutaiACY3.
DMDMi34395507.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)PRO_0000216875Add
BLAST

Proteomic databases

MaxQBiQ96HD9.
PaxDbiQ96HD9.
PRIDEiQ96HD9.

PTM databases

PhosphoSiteiQ96HD9.

Expressioni

Gene expression databases

BgeeiQ96HD9.
CleanExiHS_ACY3.
ExpressionAtlasiQ96HD9. baseline and differential.
GenevisibleiQ96HD9. HS.

Organism-specific databases

HPAiHPA039219.

Interactioni

Subunit structurei

Exists as a mixture of homodimers and homotetramer, both catalytically active (By similarity). Interacts with HCV core protein.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AAMDCQ9H7C93EBI-3916242,EBI-10308705
ASPAP453813EBI-3916242,EBI-750475
GORASP2Q9H8Y86EBI-3916242,EBI-739467
LNX1Q8TBB13EBI-3916242,EBI-739832
NOTCH2NLQ7Z3S93EBI-3916242,EBI-945833
PIAS2O759283EBI-3916242,EBI-348555
PLEKHF2Q9H8W43EBI-3916242,EBI-742388
PMM2O153053EBI-3916242,EBI-10182608

Protein-protein interaction databases

BioGridi124869. 15 interactions.
IntActiQ96HD9. 12 interactions.
STRINGi9606.ENSP00000255082.

Structurei

3D structure databases

ProteinModelPortaliQ96HD9.
SMRiQ96HD9. Positions 8-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 210210Hydrolytic domainBy similarityAdd
BLAST
Regioni70 – 712Substrate bindingBy similarity
Regioni211 – 318108Shielding domainBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2988.
GeneTreeiENSGT00390000001189.
HOGENOMiHOG000232489.
HOVERGENiHBG004172.
InParanoidiQ96HD9.
KOiK18458.
OMAiTANMGTC.
OrthoDBiEOG78SQJ6.
PhylomeDBiQ96HD9.
TreeFamiTF328708.

Family and domain databases

HAMAPiMF_00704. Aspartoacylase.
InterProiIPR016708. Aspartoacylase.
IPR007036. Aste_AspA.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF018001. Aspartoacylase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q96HD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSLPVPREP LRRVAVTGGT HGNEMSGVYL ARHWLHAPAE LQRASFSAVP
60 70 80 90 100
VLANPAATSG CRRYVDHDLN RTFTSSFLNS RPTPDDPYEV TRARELNQLL
110 120 130 140 150
GPKASGQAFD FVLDLHNTTA NMGTCLIAKS SHEVFAMHLC RHLQLQYPEL
160 170 180 190 200
SCQVFLYQRS GEESYNLDSV AKNGLGLELG PQPQGVLRAD IFSRMRTLVA
210 220 230 240 250
TVLDFIELFN QGTAFPAFEM EAYRPVGVVD FPRTEAGHLA GTVHPQLQDR
260 270 280 290 300
DFQPLQPGAP IFQMFSGEDL LYEGESTVYP VFINEAAYYE KGVAFVQTEK
310
FTFTVPAMPA LTPAPSPAS
Length:319
Mass (Da):35,241
Last modified:December 1, 2001 - v1
Checksum:i2595358AC8B5BEFB
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti8 – 81R → Q.
Corresponds to variant rs948445 [ dbSNP | Ensembl ].
VAR_048341
Natural varianti281 – 2811V → M.
Corresponds to variant rs2290959 [ dbSNP | Ensembl ].
VAR_048342

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY040761 mRNA. Translation: AAK94770.1.
AY169233 mRNA. Translation: AAN87896.1.
BC008689 mRNA. Translation: AAH08689.1.
CCDSiCCDS8175.1.
RefSeqiNP_542389.1. NM_080658.1.
UniGeneiHs.126265.

Genome annotation databases

EnsembliENST00000255082; ENSP00000255082; ENSG00000132744.
GeneIDi91703.
KEGGihsa:91703.
UCSCiuc001omq.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY040761 mRNA. Translation: AAK94770.1.
AY169233 mRNA. Translation: AAN87896.1.
BC008689 mRNA. Translation: AAH08689.1.
CCDSiCCDS8175.1.
RefSeqiNP_542389.1. NM_080658.1.
UniGeneiHs.126265.

3D structure databases

ProteinModelPortaliQ96HD9.
SMRiQ96HD9. Positions 8-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124869. 15 interactions.
IntActiQ96HD9. 12 interactions.
STRINGi9606.ENSP00000255082.

Chemistry

DrugBankiDB00128. L-Aspartic Acid.

PTM databases

PhosphoSiteiQ96HD9.

Polymorphism and mutation databases

BioMutaiACY3.
DMDMi34395507.

Proteomic databases

MaxQBiQ96HD9.
PaxDbiQ96HD9.
PRIDEiQ96HD9.

Protocols and materials databases

DNASUi91703.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000255082; ENSP00000255082; ENSG00000132744.
GeneIDi91703.
KEGGihsa:91703.
UCSCiuc001omq.3. human.

Organism-specific databases

CTDi91703.
GeneCardsiGC11M067411.
HGNCiHGNC:24104. ACY3.
HPAiHPA039219.
MIMi614413. gene.
neXtProtiNX_Q96HD9.
PharmGKBiPA134936640.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2988.
GeneTreeiENSGT00390000001189.
HOGENOMiHOG000232489.
HOVERGENiHBG004172.
InParanoidiQ96HD9.
KOiK18458.
OMAiTANMGTC.
OrthoDBiEOG78SQJ6.
PhylomeDBiQ96HD9.
TreeFamiTF328708.

Enzyme and pathway databases

BioCyciMetaCyc:HS13441-MONOMER.
ReactomeiREACT_264461. Aflatoxin activation and detoxification.

Miscellaneous databases

GenomeRNAii91703.
NextBioi77411.
PROiQ96HD9.
SOURCEiSearch...

Gene expression databases

BgeeiQ96HD9.
CleanExiHS_ACY3.
ExpressionAtlasiQ96HD9. baseline and differential.
GenevisibleiQ96HD9. HS.

Family and domain databases

HAMAPiMF_00704. Aspartoacylase.
InterProiIPR016708. Aspartoacylase.
IPR007036. Aste_AspA.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF018001. Aspartoacylase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel aspartoacylase homolog (ACY-3) in human and mouse kidneys."
    Huang C.-H., Chen H., Peng J., Chen Y.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Pushkin A., Carpenito G., Abuladze N., Newman D., Kurtz I.
    Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  4. "Identification of a novel protein binding to hepatitis C virus core protein."
    Chen Y.R., Chen T.Y., Zhang S.L., Lin S.M., Zhao Y.R., Ye F., Zhang X., Shi L., Dang S.S., Liu M.
    J. Gastroenterol. Hepatol. 24:1300-1304(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HCV CORE PROTEIN, SUBCELLULAR LOCATION.
  5. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiACY3_HUMAN
AccessioniPrimary (citable) accession number: Q96HD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.