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Q96HA8

- NTAQ1_HUMAN

UniProt

Q96HA8 - NTAQ1_HUMAN

Protein

Protein N-terminal glutamine amidohydrolase

Gene

WDYHV1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (06 Mar 2007)
      Previous versions | rss
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    Functioni

    Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei28 – 281By similarity
    Active sitei81 – 811By similarity
    Active sitei97 – 971By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein-N-terminal glutamine amidohydrolase activity Source: UniProtKB

    GO - Biological processi

    1. cellular protein modification process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein N-terminal glutamine amidohydrolase (EC:3.5.1.-)
    Alternative name(s):
    Protein NH2-terminal glutamine deamidase
    Short name:
    N-terminal Gln amidase
    Short name:
    Nt(Q)-amidase
    WDYHV motif-containing protein 1
    Gene namesi
    Name:WDYHV1
    Synonyms:C8orf32, NTAQ1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:25490. WDYHV1.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA164727566.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 205205Protein N-terminal glutamine amidohydrolasePRO_0000279409Add
    BLAST

    Proteomic databases

    MaxQBiQ96HA8.
    PaxDbiQ96HA8.
    PRIDEiQ96HA8.

    PTM databases

    PhosphoSiteiQ96HA8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ96HA8.
    BgeeiQ96HA8.
    CleanExiHS_WDYHV1.
    GenevestigatoriQ96HA8.

    Organism-specific databases

    HPAiHPA024823.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HPRT1P004923EBI-741158,EBI-748210
    HSD17B14Q9BPX13EBI-741158,EBI-742664
    JUPP149233EBI-741158,EBI-702484
    NME1P155313EBI-741158,EBI-741141
    RABAC1Q9UI143EBI-741158,EBI-712367
    TRIP13Q156453EBI-741158,EBI-358993

    Protein-protein interaction databases

    BioGridi120405. 19 interactions.
    IntActiQ96HA8. 19 interactions.
    MINTiMINT-1443224.
    STRINGi9606.ENSP00000287387.

    Structurei

    Secondary structure

    1
    205
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi18 – 203
    Helixi28 – 4114
    Beta strandi42 – 454
    Helixi47 – 493
    Beta strandi50 – 567
    Beta strandi62 – 676
    Beta strandi76 – 794
    Beta strandi81 – 888
    Turni89 – 913
    Beta strandi92 – 965
    Beta strandi100 – 1023
    Beta strandi104 – 1074
    Helixi108 – 1147
    Helixi124 – 1263
    Beta strandi129 – 1346
    Helixi135 – 1417
    Helixi147 – 1493
    Beta strandi152 – 1543
    Beta strandi156 – 1583
    Helixi176 – 1794
    Beta strandi185 – 1928
    Helixi193 – 2008

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3C9QX-ray1.50A2-205[»]
    ProteinModelPortaliQ96HA8.
    SMRiQ96HA8. Positions 8-202.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ96HA8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NTAQ1 family.Curated

    Phylogenomic databases

    eggNOGiNOG282583.
    HOGENOMiHOG000007890.
    InParanoidiQ96HA8.
    OMAiNSCYCEE.
    OrthoDBiEOG70PC0N.
    PhylomeDBiQ96HA8.
    TreeFamiTF105807.

    Family and domain databases

    Gene3Di3.10.620.10. 1 hit.
    InterProiIPR023128. Prot_N_Gln_amidohydro_ab_roll.
    [Graphical view]
    PfamiPF09764. Nt_Gln_amidase. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96HA8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEGNGPAAVH YQPASPPRDA CVYSSCYCEE NIWKLCEYIK NHDQYPLEEC    50
    YAVFISNERK MIPIWKQQAR PGDGPVIWDY HVVLLHVSSG GQNFIYDLDT 100
    VLPFPCLFDT YVEDAFKSDD DIHPQFRRKF RVIRADSYLK NFASDRSHMK 150
    DSSGNWREPP PPYPCIETGD SKMNLNDFIS MDPKVGWGAV YTLSEFTHRF 200
    GSKNC 205
    Length:205
    Mass (Da):23,680
    Last modified:March 6, 2007 - v2
    Checksum:iF858AB0C73928CA9
    GO
    Isoform 2 (identifier: Q96HA8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-60: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:145
    Mass (Da):16,736
    Checksum:iAC61BBE11D3642F1
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti32 – 321I → V.1 Publication
    Corresponds to variant rs6999234 [ dbSNP | Ensembl ].
    VAR_030882
    Natural varianti93 – 931N → S.1 Publication
    Corresponds to variant rs7014678 [ dbSNP | Ensembl ].
    VAR_030883
    Natural varianti116 – 1161F → I.1 Publication
    Corresponds to variant rs6470147 [ dbSNP | Ensembl ].
    VAR_030884
    Natural varianti134 – 1341R → C.1 Publication
    Corresponds to variant rs3824250 [ dbSNP | Ensembl ].
    VAR_030885

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6060Missing in isoform 2. 1 PublicationVSP_055268Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK001066 mRNA. Translation: BAA91488.1.
    AK293492 mRNA. Translation: BAG56979.1.
    AC021305 Genomic DNA. No translation available.
    CH471060 Genomic DNA. Translation: EAW92038.1.
    BC008781 mRNA. Translation: AAH08781.1.
    CCDSiCCDS6344.1. [Q96HA8-1]
    CCDS64965.1. [Q96HA8-2]
    RefSeqiNP_001269953.1. NM_001283024.1.
    NP_060494.1. NM_018024.2.
    UniGeneiHs.18029.

    Genome annotation databases

    GeneIDi55093.
    KEGGihsa:55093.
    UCSCiuc003yqn.1. human. [Q96HA8-1]

    Polymorphism databases

    DMDMi152112225.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK001066 mRNA. Translation: BAA91488.1 .
    AK293492 mRNA. Translation: BAG56979.1 .
    AC021305 Genomic DNA. No translation available.
    CH471060 Genomic DNA. Translation: EAW92038.1 .
    BC008781 mRNA. Translation: AAH08781.1 .
    CCDSi CCDS6344.1. [Q96HA8-1 ]
    CCDS64965.1. [Q96HA8-2 ]
    RefSeqi NP_001269953.1. NM_001283024.1.
    NP_060494.1. NM_018024.2.
    UniGenei Hs.18029.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3C9Q X-ray 1.50 A 2-205 [» ]
    ProteinModelPortali Q96HA8.
    SMRi Q96HA8. Positions 8-202.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120405. 19 interactions.
    IntActi Q96HA8. 19 interactions.
    MINTi MINT-1443224.
    STRINGi 9606.ENSP00000287387.

    PTM databases

    PhosphoSitei Q96HA8.

    Polymorphism databases

    DMDMi 152112225.

    Proteomic databases

    MaxQBi Q96HA8.
    PaxDbi Q96HA8.
    PRIDEi Q96HA8.

    Protocols and materials databases

    DNASUi 55093.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 55093.
    KEGGi hsa:55093.
    UCSCi uc003yqn.1. human. [Q96HA8-1 ]

    Organism-specific databases

    CTDi 55093.
    GeneCardsi GC08P124428.
    HGNCi HGNC:25490. WDYHV1.
    HPAi HPA024823.
    neXtProti NX_Q96HA8.
    PharmGKBi PA164727566.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG282583.
    HOGENOMi HOG000007890.
    InParanoidi Q96HA8.
    OMAi NSCYCEE.
    OrthoDBi EOG70PC0N.
    PhylomeDBi Q96HA8.
    TreeFami TF105807.

    Miscellaneous databases

    EvolutionaryTracei Q96HA8.
    GeneWikii C8orf32.
    GenomeRNAii 55093.
    NextBioi 35471129.
    PROi Q96HA8.

    Gene expression databases

    ArrayExpressi Q96HA8.
    Bgeei Q96HA8.
    CleanExi HS_WDYHV1.
    Genevestigatori Q96HA8.

    Family and domain databases

    Gene3Di 3.10.620.10. 1 hit.
    InterProi IPR023128. Prot_N_Gln_amidohydro_ab_roll.
    [Graphical view ]
    Pfami PF09764. Nt_Gln_amidase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Cerebellum and Embryo.
    2. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-32; SER-93; ILE-116 AND CYS-134.
      Tissue: Uterus.
    5. "Crystal structure of the uncharacterized human protein c8orf32 with bound peptide."
      Center for eukaryotic structural genomics (CESG)
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 2-205 IN COMPLEX WITH SER-THR-ALA TRIPEPTIDE, SUBUNIT.

    Entry informationi

    Entry nameiNTAQ1_HUMAN
    AccessioniPrimary (citable) accession number: Q96HA8
    Secondary accession number(s): B4DE68, Q9NW95
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2007
    Last sequence update: March 6, 2007
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3