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Q96HA8 (NTAQ1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein N-terminal glutamine amidohydrolase

EC=3.5.1.-
Alternative name(s):
Protein NH2-terminal glutamine deamidase
Short name=N-terminal Gln amidase
Short name=Nt(Q)-amidase
WDYHV motif-containing protein 1
Gene names
Name:WDYHV1
Synonyms:C8orf32, NTAQ1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length205 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine By similarity.

Subunit structure

Monomer. Ref.5

Subcellular location

Cytoplasmcytosol By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the NTAQ1 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96HA8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96HA8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 205205Protein N-terminal glutamine amidohydrolase
PRO_0000279409

Sites

Active site281 By similarity
Active site811 By similarity
Active site971 By similarity

Natural variations

Alternative sequence1 – 6060Missing in isoform 2.
VSP_055268
Natural variant321I → V. Ref.4
Corresponds to variant rs6999234 [ dbSNP | Ensembl ].
VAR_030882
Natural variant931N → S. Ref.4
Corresponds to variant rs7014678 [ dbSNP | Ensembl ].
VAR_030883
Natural variant1161F → I. Ref.4
Corresponds to variant rs6470147 [ dbSNP | Ensembl ].
VAR_030884
Natural variant1341R → C. Ref.4
Corresponds to variant rs3824250 [ dbSNP | Ensembl ].
VAR_030885

Secondary structure

...................................... 205
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 6, 2007. Version 2.
Checksum: F858AB0C73928CA9

FASTA20523,680
        10         20         30         40         50         60 
MEGNGPAAVH YQPASPPRDA CVYSSCYCEE NIWKLCEYIK NHDQYPLEEC YAVFISNERK 

        70         80         90        100        110        120 
MIPIWKQQAR PGDGPVIWDY HVVLLHVSSG GQNFIYDLDT VLPFPCLFDT YVEDAFKSDD 

       130        140        150        160        170        180 
DIHPQFRRKF RVIRADSYLK NFASDRSHMK DSSGNWREPP PPYPCIETGD SKMNLNDFIS 

       190        200 
MDPKVGWGAV YTLSEFTHRF GSKNC 

« Hide

Isoform 2 [UniParc].

Checksum: AC61BBE11D3642F1
Show »

FASTA14516,736

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Cerebellum and Embryo.
[2]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-32; SER-93; ILE-116 AND CYS-134.
Tissue: Uterus.
[5]"Crystal structure of the uncharacterized human protein c8orf32 with bound peptide."
Center for eukaryotic structural genomics (CESG)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 2-205 IN COMPLEX WITH SER-THR-ALA TRIPEPTIDE, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK001066 mRNA. Translation: BAA91488.1.
AK293492 mRNA. Translation: BAG56979.1.
AC021305 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW92038.1.
BC008781 mRNA. Translation: AAH08781.1.
CCDSCCDS6344.1.
RefSeqNP_060494.1. NM_018024.2.
UniGeneHs.18029.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3C9QX-ray1.50A2-205[»]
ProteinModelPortalQ96HA8.
SMRQ96HA8. Positions 8-202.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120405. 19 interactions.
IntActQ96HA8. 19 interactions.
MINTMINT-1443224.
STRING9606.ENSP00000287387.

PTM databases

PhosphoSiteQ96HA8.

Polymorphism databases

DMDM152112225.

Proteomic databases

MaxQBQ96HA8.
PaxDbQ96HA8.
PRIDEQ96HA8.

Protocols and materials databases

DNASU55093.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000287387; ENSP00000287387; ENSG00000156795.
ENST00000523984; ENSP00000430427; ENSG00000156795.
GeneID55093.
KEGGhsa:55093.
UCSCuc003yqn.1. human.

Organism-specific databases

CTD55093.
GeneCardsGC08P124428.
HGNCHGNC:25490. WDYHV1.
HPAHPA024823.
neXtProtNX_Q96HA8.
PharmGKBPA164727566.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG282583.
HOGENOMHOG000007890.
InParanoidQ96HA8.
OMANSCYCEE.
OrthoDBEOG70PC0N.
PhylomeDBQ96HA8.
TreeFamTF105807.

Gene expression databases

ArrayExpressQ96HA8.
BgeeQ96HA8.
CleanExHS_WDYHV1.
GenevestigatorQ96HA8.

Family and domain databases

Gene3D3.10.620.10. 1 hit.
InterProIPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view]
PfamPF09764. Nt_Gln_amidase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ96HA8.
GeneWikiC8orf32.
GenomeRNAi55093.
NextBio58673.
PROQ96HA8.

Entry information

Entry nameNTAQ1_HUMAN
AccessionPrimary (citable) accession number: Q96HA8
Secondary accession number(s): B4DE68, Q9NW95
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: July 9, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM