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Protein

Protein N-terminal glutamine amidohydrolase

Gene

WDYHV1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281By similarity
Active sitei81 – 811By similarity
Active sitei97 – 971By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-terminal glutamine amidohydrolase (EC:3.5.1.-)
Alternative name(s):
Protein NH2-terminal glutamine deamidase
Short name:
N-terminal Gln amidase
Short name:
Nt(Q)-amidase
WDYHV motif-containing protein 1
Gene namesi
Name:WDYHV1
Synonyms:C8orf32, NTAQ1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:25490. WDYHV1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164727566.

Polymorphism and mutation databases

BioMutaiWDYHV1.
DMDMi152112225.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Protein N-terminal glutamine amidohydrolasePRO_0000279409Add
BLAST

Proteomic databases

MaxQBiQ96HA8.
PaxDbiQ96HA8.
PRIDEiQ96HA8.

PTM databases

PhosphoSiteiQ96HA8.

Expressioni

Gene expression databases

BgeeiQ96HA8.
CleanExiHS_WDYHV1.
ExpressionAtlasiQ96HA8. baseline and differential.
GenevisibleiQ96HA8. HS.

Organism-specific databases

HPAiHPA024823.
HPA053680.

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
I4AY873EBI-741158,EBI-10287234
ACTBP607093EBI-741158,EBI-353944
ACTG1P632613EBI-741158,EBI-351292
AMOTQ4VCS5-23EBI-741158,EBI-3891843
AMOTL2Q9Y2J4-43EBI-741158,EBI-10187270
APIPQ96GX93EBI-741158,EBI-359248
ASLP044243EBI-741158,EBI-750131
BIRC2Q134903EBI-741158,EBI-514538
BLMHQ138673EBI-741158,EBI-718504
C1orf50Q9BV193EBI-741158,EBI-2874661
CAPN3P208073EBI-741158,EBI-5655000
CCDC102BA1A4H13EBI-741158,EBI-10171570
CCDC184Q52MB23EBI-741158,EBI-10179526
CDAP323203EBI-741158,EBI-9250559
CDR2Q018503EBI-741158,EBI-1181367
CRYAAP024893EBI-741158,EBI-6875961
CTHP329293EBI-741158,EBI-749763
DAB1O755533EBI-741158,EBI-7875264
DHPSP493663EBI-741158,EBI-741925
EDARADDQ8WWZ33EBI-741158,EBI-2949647
EIF2B1Q142323EBI-741158,EBI-491065
ETV6P412123EBI-741158,EBI-1372759
FTH1Q6NZ443EBI-741158,EBI-10180219
GAS7O608613EBI-741158,EBI-2683717
GMDSO605473EBI-741158,EBI-746373
GOLGA2Q083793EBI-741158,EBI-618309
HEL-S-182mPV9HW603EBI-741158,EBI-10180762
HEL-S-70V9HW803EBI-741158,EBI-10175326
HPRT1P004926EBI-741158,EBI-748210
HSD17B14Q9BPX16EBI-741158,EBI-742664
JUPP149233EBI-741158,EBI-702484
KCNH1O952593EBI-741158,EBI-2909270
KCTD1Q719H93EBI-741158,EBI-9027502
KLHL12Q53G593EBI-741158,EBI-740929
KRT31Q153233EBI-741158,EBI-948001
KRTAP10-5P603703EBI-741158,EBI-10172150
KRTAP10-7P604093EBI-741158,EBI-10172290
KRTAP4-2Q9BYR53EBI-741158,EBI-10172511
KRTAP5-9P263713EBI-741158,EBI-3958099
KRTAP9-2Q9BYQ43EBI-741158,EBI-1044640
KRTAP9-4Q9BYQ23EBI-741158,EBI-10185730
LONRF1Q17RB83EBI-741158,EBI-2341787
LZTFL1Q9NQ483EBI-741158,EBI-2824799
LZTS2Q9BRK43EBI-741158,EBI-741037
MAGEA11P43364-23EBI-741158,EBI-10178634
MARCH10Q8NA823EBI-741158,EBI-2341554
MTUS2Q5JR593EBI-741158,EBI-742948
NECAB2H3BTW23EBI-741158,EBI-10172876
NME1P155313EBI-741158,EBI-741141
NPLQ9BXD53EBI-741158,EBI-10287915
NUDT14O958483EBI-741158,EBI-536866
PNMA1Q8ND903EBI-741158,EBI-302345
PNMA5Q96PV43EBI-741158,EBI-10171633
PPCDCQ96CD23EBI-741158,EBI-724333
PRMT1Q998733EBI-741158,EBI-78738
PRPS2P119083EBI-741158,EBI-4290895
PS1TP5BP1Q1KLZ03EBI-741158,EBI-9978131
PYGMP112173EBI-741158,EBI-357469
RABAC1Q9UI146EBI-741158,EBI-712367
RAD54LQ926983EBI-741158,EBI-5333483
RBPMSQ930623EBI-741158,EBI-740322
RPIAP492474EBI-741158,EBI-744831
SEPT3Q9UH033EBI-741158,EBI-727037
SIAH1Q8IUQ43EBI-741158,EBI-747107
STX11O755583EBI-741158,EBI-714135
THAP1Q9NVV94EBI-741158,EBI-741515
TMEM239Q8WW343EBI-741158,EBI-9675724
TNRA1L3063EBI-741158,EBI-10182881
TOLLIPQ9H0E23EBI-741158,EBI-74615
TRIM27P143733EBI-741158,EBI-719493
TRIM54Q9BYV23EBI-741158,EBI-2130429
TRIP13Q156453EBI-741158,EBI-358993
TSC22D1Q157143EBI-741158,EBI-712609
VAC14Q08AM63EBI-741158,EBI-2107455
ZBTB8AQ96BR93EBI-741158,EBI-742740
ZNF341Q9BYN73EBI-741158,EBI-9089622

Protein-protein interaction databases

BioGridi120405. 88 interactions.
IntActiQ96HA8. 89 interactions.
MINTiMINT-1443224.
STRINGi9606.ENSP00000287387.

Structurei

Secondary structure

1
205
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 203Combined sources
Helixi28 – 4114Combined sources
Beta strandi42 – 454Combined sources
Helixi47 – 493Combined sources
Beta strandi50 – 567Combined sources
Beta strandi62 – 676Combined sources
Beta strandi76 – 794Combined sources
Beta strandi81 – 888Combined sources
Turni89 – 913Combined sources
Beta strandi92 – 965Combined sources
Beta strandi100 – 1023Combined sources
Beta strandi104 – 1074Combined sources
Helixi108 – 1147Combined sources
Helixi124 – 1263Combined sources
Beta strandi129 – 1346Combined sources
Helixi135 – 1417Combined sources
Helixi147 – 1493Combined sources
Beta strandi152 – 1543Combined sources
Beta strandi156 – 1583Combined sources
Helixi176 – 1794Combined sources
Beta strandi185 – 1928Combined sources
Helixi193 – 2008Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C9QX-ray1.50A2-205[»]
4W79X-ray1.50A2-202[»]
ProteinModelPortaliQ96HA8.
SMRiQ96HA8. Positions 2-202.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96HA8.

Family & Domainsi

Sequence similaritiesi

Belongs to the NTAQ1 family.Curated

Phylogenomic databases

eggNOGiNOG282583.
GeneTreeiENSGT00390000014398.
HOGENOMiHOG000007890.
InParanoidiQ96HA8.
OMAiVIHAPVF.
OrthoDBiEOG70PC0N.
PhylomeDBiQ96HA8.
TreeFamiTF105807.

Family and domain databases

Gene3Di3.10.620.10. 1 hit.
InterProiIPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view]
PfamiPF09764. Nt_Gln_amidase. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96HA8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGNGPAAVH YQPASPPRDA CVYSSCYCEE NIWKLCEYIK NHDQYPLEEC
60 70 80 90 100
YAVFISNERK MIPIWKQQAR PGDGPVIWDY HVVLLHVSSG GQNFIYDLDT
110 120 130 140 150
VLPFPCLFDT YVEDAFKSDD DIHPQFRRKF RVIRADSYLK NFASDRSHMK
160 170 180 190 200
DSSGNWREPP PPYPCIETGD SKMNLNDFIS MDPKVGWGAV YTLSEFTHRF

GSKNC
Length:205
Mass (Da):23,680
Last modified:March 6, 2007 - v2
Checksum:iF858AB0C73928CA9
GO
Isoform 2 (identifier: Q96HA8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.

Note: No experimental confirmation available.
Show »
Length:145
Mass (Da):16,736
Checksum:iAC61BBE11D3642F1
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti32 – 321I → V.1 Publication
Corresponds to variant rs6999234 [ dbSNP | Ensembl ].
VAR_030882
Natural varianti93 – 931N → S.1 Publication
Corresponds to variant rs7014678 [ dbSNP | Ensembl ].
VAR_030883
Natural varianti116 – 1161F → I.1 Publication
Corresponds to variant rs6470147 [ dbSNP | Ensembl ].
VAR_030884
Natural varianti134 – 1341R → C.1 Publication
Corresponds to variant rs3824250 [ dbSNP | Ensembl ].
VAR_030885

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6060Missing in isoform 2. 1 PublicationVSP_055268Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001066 mRNA. Translation: BAA91488.1.
AK293492 mRNA. Translation: BAG56979.1.
AC021305 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW92038.1.
BC008781 mRNA. Translation: AAH08781.1.
CCDSiCCDS6344.1. [Q96HA8-1]
CCDS64965.1. [Q96HA8-2]
RefSeqiNP_001269953.1. NM_001283024.1. [Q96HA8-2]
NP_060494.1. NM_018024.2. [Q96HA8-1]
UniGeneiHs.18029.

Genome annotation databases

EnsembliENST00000287387; ENSP00000287387; ENSG00000156795. [Q96HA8-1]
ENST00000523984; ENSP00000430427; ENSG00000156795. [Q96HA8-2]
GeneIDi55093.
KEGGihsa:55093.
UCSCiuc003yqn.1. human. [Q96HA8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001066 mRNA. Translation: BAA91488.1.
AK293492 mRNA. Translation: BAG56979.1.
AC021305 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW92038.1.
BC008781 mRNA. Translation: AAH08781.1.
CCDSiCCDS6344.1. [Q96HA8-1]
CCDS64965.1. [Q96HA8-2]
RefSeqiNP_001269953.1. NM_001283024.1. [Q96HA8-2]
NP_060494.1. NM_018024.2. [Q96HA8-1]
UniGeneiHs.18029.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C9QX-ray1.50A2-205[»]
4W79X-ray1.50A2-202[»]
ProteinModelPortaliQ96HA8.
SMRiQ96HA8. Positions 2-202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120405. 88 interactions.
IntActiQ96HA8. 89 interactions.
MINTiMINT-1443224.
STRINGi9606.ENSP00000287387.

PTM databases

PhosphoSiteiQ96HA8.

Polymorphism and mutation databases

BioMutaiWDYHV1.
DMDMi152112225.

Proteomic databases

MaxQBiQ96HA8.
PaxDbiQ96HA8.
PRIDEiQ96HA8.

Protocols and materials databases

DNASUi55093.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000287387; ENSP00000287387; ENSG00000156795. [Q96HA8-1]
ENST00000523984; ENSP00000430427; ENSG00000156795. [Q96HA8-2]
GeneIDi55093.
KEGGihsa:55093.
UCSCiuc003yqn.1. human. [Q96HA8-1]

Organism-specific databases

CTDi55093.
GeneCardsiGC08P124428.
HGNCiHGNC:25490. WDYHV1.
HPAiHPA024823.
HPA053680.
neXtProtiNX_Q96HA8.
PharmGKBiPA164727566.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG282583.
GeneTreeiENSGT00390000014398.
HOGENOMiHOG000007890.
InParanoidiQ96HA8.
OMAiVIHAPVF.
OrthoDBiEOG70PC0N.
PhylomeDBiQ96HA8.
TreeFamiTF105807.

Miscellaneous databases

EvolutionaryTraceiQ96HA8.
GeneWikiiC8orf32.
GenomeRNAii55093.
NextBioi35471129.
PROiQ96HA8.

Gene expression databases

BgeeiQ96HA8.
CleanExiHS_WDYHV1.
ExpressionAtlasiQ96HA8. baseline and differential.
GenevisibleiQ96HA8. HS.

Family and domain databases

Gene3Di3.10.620.10. 1 hit.
InterProiIPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view]
PfamiPF09764. Nt_Gln_amidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Cerebellum and Embryo.
  2. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-32; SER-93; ILE-116 AND CYS-134.
    Tissue: Uterus.
  5. "Crystal structure of the uncharacterized human protein c8orf32 with bound peptide."
    Center for eukaryotic structural genomics (CESG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 2-205 IN COMPLEX WITH SER-THR-ALA TRIPEPTIDE, SUBUNIT.

Entry informationi

Entry nameiNTAQ1_HUMAN
AccessioniPrimary (citable) accession number: Q96HA8
Secondary accession number(s): B4DE68, Q9NW95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: June 24, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.