Q96HA8 (NTAQ1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein N-terminal glutamine amidohydrolase
EC=3.5.1.-

Alternative name(s):
Protein NH2-terminal glutamine deamidase
Short name=N-terminal Gln amidase
Short name=Nt(Q)-amidase
WDYHV motif-containing protein 1

Gene names

Name:	WDYHV1
Synonyms:	C8orf32, NTAQ1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine By similarity.
Subunit structure	Monomer. Ref.3
Subcellular location	Cytoplasm › cytosol By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the NTAQ1 family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein modification process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	protein-N-terminal glutamine amidohydrolase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HPRT1	P00492	3	EBI-741158,EBI-748210
HSD17B14	Q9BPX1	3	EBI-741158,EBI-742664
JUP	P14923	3	EBI-741158,EBI-702484
NME1	P15531	3	EBI-741158,EBI-741141
RABAC1	Q9UI14	3	EBI-741158,EBI-712367
TRIP13	Q15645	3	EBI-741158,EBI-358993

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 205

205

Protein N-terminal glutamine amidohydrolase

PRO_0000279409

Sites

Active site

28

1

By similarity

Active site

81

1

By similarity

Active site

97

1

By similarity

Natural variations

Natural variant

32

1

I → V. Ref.2
Corresponds to variant rs6999234 [ dbSNP | Ensembl ].

VAR_030882

Natural variant

93

1

N → S. Ref.2
Corresponds to variant rs7014678 [ dbSNP | Ensembl ].

VAR_030883

Natural variant

116

1

F → I. Ref.2
Corresponds to variant rs6470147 [ dbSNP | Ensembl ].

VAR_030884

Natural variant

134

1

R → C. Ref.2
Corresponds to variant rs3824250 [ dbSNP | Ensembl ].

VAR_030885

Secondary structure

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205

Helix Strand Turn

Details...

Helix

18 – 20

3

Helix

28 – 41

14

Beta strand

42 – 45

4

Helix

47 – 49

3

Beta strand

50 – 56

7

Beta strand

62 – 67

6

Beta strand

76 – 79

4

Beta strand

81 – 88

8

Turn

89 – 91

3

Beta strand

92 – 96

5

Beta strand

100 – 102

3

Beta strand

104 – 107

4

Helix

108 – 114

7

Helix

124 – 126

3

Beta strand

129 – 134

6

Helix

135 – 141

7

Helix

147 – 149

3

Beta strand

152 – 154

3

Beta strand

156 – 158

3

Helix

176 – 179

4

Beta strand

185 – 192

8

Helix

193 – 200

8

Sequences

	Sequence		Length	Mass (Da)
	Q96HA8 [UniParc]. Last modified March 6, 2007. Version 2. Checksum: F858AB0C73928CA9	FASTA	205	23,680
	10 20 30 40 50 60 MEGNGPAAVH YQPASPPRDA CVYSSCYCEE NIWKLCEYIK NHDQYPLEEC YAVFISNERK 70 80 90 100 110 120 MIPIWKQQAR PGDGPVIWDY HVVLLHVSSG GQNFIYDLDT VLPFPCLFDT YVEDAFKSDD 130 140 150 160 170 180 DIHPQFRRKF RVIRADSYLK NFASDRSHMK DSSGNWREPP PPYPCIETGD SKMNLNDFIS 190 200 MDPKVGWGAV YTLSEFTHRF GSKNC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS VAL-32; SER-93; ILE-116 AND CYS-134. Tissue: Uterus.
[3]	"Crystal structure of the uncharacterized human protein c8orf32 with bound peptide." Center for eukaryotic structural genomics (CESG) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 2-205 IN COMPLEX WITH SER-THR-ALA TRIPEPTIDE, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK001066 mRNA. Translation: BAA91488.1.
BC008781 mRNA. Translation: AAH08781.1.

IPI

IPI00329756.

RefSeq

NP_060494.1. NM_018024.1.

UniGene

Hs.18029.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3C9Q	X-ray	1.50	A	2-205	[»]

ProteinModelPortal

Q96HA8.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96HA8. 18 interactions.

MINT

MINT-1443224.

STRING

9606.ENSP00000287387.

PTM databases

PhosphoSite

Q96HA8.

Polymorphism databases

DMDM

152112225.

Proteomic databases

PaxDb

Q96HA8.

PRIDE

Q96HA8.

Protocols and materials databases

DNASU

55093.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000287387; ENSP00000287387; ENSG00000156795.

GeneID

55093.

KEGG

hsa:55093.

UCSC

uc003yqn.1. human.

Organism-specific databases

CTD

55093.

GeneCards

GC08P124428.

HGNC

HGNC:25490. WDYHV1.

HPA

HPA024823.

neXtProt

NX_Q96HA8.

PharmGKB

PA164727566.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG282583.

HOGENOM

HOG000007890.

InParanoid

Q96HA8.

OMA

VPLWRQK.

OrthoDB

EOG4HQDKH.

PhylomeDB

Q96HA8.

Gene expression databases

ArrayExpress

Q96HA8.

Bgee

Q96HA8.

CleanEx

HS_WDYHV1.

Genevestigator

Q96HA8.

Family and domain databases

Gene3D

3.10.620.10. 1 hit.

InterPro

IPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view]

Pfam

PF09764. Nt_Gln_amidase. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q96HA8.

GenomeRNAi

55093.

NextBio

58673.

Entry information

Entry name

NTAQ1_HUMAN

Accession

Primary (citable) accession number: Q96HA8
Secondary accession number(s): Q9NW95

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.