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Protein

Protein N-terminal glutamine amidohydrolase

Gene

WDYHV1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei28By similarity1
Active sitei81By similarity1
Active sitei97By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-terminal glutamine amidohydrolase (EC:3.5.1.-)
Alternative name(s):
Protein NH2-terminal glutamine deamidase
Short name:
N-terminal Gln amidase
Short name:
Nt(Q)-amidase
WDYHV motif-containing protein 1
Gene namesi
Name:WDYHV1
Synonyms:C8orf32, NTAQ1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:25490. WDYHV1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000156795.
PharmGKBiPA164727566.

Polymorphism and mutation databases

BioMutaiWDYHV1.
DMDMi152112225.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002794091 – 205Protein N-terminal glutamine amidohydrolaseAdd BLAST205

Proteomic databases

EPDiQ96HA8.
MaxQBiQ96HA8.
PaxDbiQ96HA8.
PeptideAtlasiQ96HA8.
PRIDEiQ96HA8.

PTM databases

iPTMnetiQ96HA8.
PhosphoSitePlusiQ96HA8.

Expressioni

Gene expression databases

BgeeiENSG00000156795.
CleanExiHS_WDYHV1.
ExpressionAtlasiQ96HA8. baseline and differential.
GenevisibleiQ96HA8. HS.

Organism-specific databases

HPAiHPA024823.
HPA053680.

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTBP607093EBI-741158,EBI-353944
ACTG1P632615EBI-741158,EBI-351292
AMOTQ4VCS5-23EBI-741158,EBI-3891843
AMOTL2Q9Y2J4-43EBI-741158,EBI-10187270
APIPQ96GX93EBI-741158,EBI-359248
ASLP044245EBI-741158,EBI-750131
BIRC2Q134903EBI-741158,EBI-514538
BLMHQ138673EBI-741158,EBI-718504
C1orf50Q9BV195EBI-741158,EBI-2874661
CAPN3P208073EBI-741158,EBI-5655000
CCDC102BA1A4H13EBI-741158,EBI-10171570
CCDC184Q52MB23EBI-741158,EBI-10179526
CDAP323205EBI-741158,EBI-9250559
CDR2Q018503EBI-741158,EBI-1181367
CEP55Q53EZ44EBI-741158,EBI-747776
COG6Q9Y2V74EBI-741158,EBI-3866319
COILP384323EBI-741158,EBI-945751
CRYAAP024895EBI-741158,EBI-6875961
CTHP329293EBI-741158,EBI-749763
DAB1O755533EBI-741158,EBI-7875264
DCTPP1Q9H7733EBI-741158,EBI-723569
DHPSP493663EBI-741158,EBI-741925
EDARADDQ8WWZ33EBI-741158,EBI-2949647
EIF2B1Q142325EBI-741158,EBI-491065
ETV6P412123EBI-741158,EBI-1372759
FTH1Q6NZ443EBI-741158,EBI-10180219
GAS7O608613EBI-741158,EBI-2683717
GAS7O60861-14EBI-741158,EBI-11745923
GMDSO605475EBI-741158,EBI-746373
GOLGA2A0A0C4DGS54EBI-741158,EBI-11522202
GOLGA2Q083793EBI-741158,EBI-618309
HEL-S-182mPV9HW603EBI-741158,EBI-10180762
HEL-S-70V9HW803EBI-741158,EBI-10175326
HPRT1P004928EBI-741158,EBI-748210
HSD17B14Q9BPX110EBI-741158,EBI-742664
JUPP149233EBI-741158,EBI-702484
KCNH1O952593EBI-741158,EBI-2909270
KCTD1Q719H93EBI-741158,EBI-9027502
KLHL12Q53G597EBI-741158,EBI-740929
KRT31Q153233EBI-741158,EBI-948001
KRTAP10-5P603703EBI-741158,EBI-10172150
KRTAP10-7P604093EBI-741158,EBI-10172290
KRTAP4-2Q9BYR53EBI-741158,EBI-10172511
KRTAP5-9P263715EBI-741158,EBI-3958099
KRTAP9-2Q9BYQ43EBI-741158,EBI-1044640
KRTAP9-4Q9BYQ23EBI-741158,EBI-10185730
LONRF1Q17RB83EBI-741158,EBI-2341787
LZTFL1Q9NQ483EBI-741158,EBI-2824799
LZTS2Q9BRK43EBI-741158,EBI-741037
MAGEA11P43364-23EBI-741158,EBI-10178634
MAGEA2BP433564EBI-741158,EBI-5650739
MARCH10Q8NA823EBI-741158,EBI-2341554
MIFI4AY873EBI-741158,EBI-10287234
MTUS2Q5JR593EBI-741158,EBI-742948
MTUS2Q5JR59-34EBI-741158,EBI-11522433
NAGKQ9UJ70-24EBI-741158,EBI-11526455
NECAB2H3BTW27EBI-741158,EBI-10172876
NME1P155313EBI-741158,EBI-741141
NPLQ9BXD53EBI-741158,EBI-10287915
NT5C1AQ9BXI34EBI-741158,EBI-10441581
NUDT14O958483EBI-741158,EBI-536866
PCBD1P614573EBI-741158,EBI-740475
PNMA1Q8ND905EBI-741158,EBI-302345
PNMA5Q96PV45EBI-741158,EBI-10171633
PPCDCQ96CD25EBI-741158,EBI-724333
PRMT1Q998733EBI-741158,EBI-78738
PRPS2P119083EBI-741158,EBI-4290895
PS1TP5BP1Q1KLZ03EBI-741158,EBI-9978131
PSME3P612894EBI-741158,EBI-355546
PYGMP112173EBI-741158,EBI-357469
RABAC1Q9UI148EBI-741158,EBI-712367
RAD54LQ926983EBI-741158,EBI-5333483
RBPMSQ930623EBI-741158,EBI-740322
ROPN1Q9HAT04EBI-741158,EBI-1378139
RPIAP492476EBI-741158,EBI-744831
SEPT3Q9UH033EBI-741158,EBI-727037
SFNP319473EBI-741158,EBI-476295
SIAH1Q8IUQ43EBI-741158,EBI-747107
SPERTQ8NA61-24EBI-741158,EBI-11524851
STX11O755583EBI-741158,EBI-714135
TCL1AP562794EBI-741158,EBI-749995
THAP1Q9NVV96EBI-741158,EBI-741515
TMEM239Q8WW343EBI-741158,EBI-9675724
TNRA1L3063EBI-741158,EBI-10182881
TOLLIPQ9H0E23EBI-741158,EBI-74615
TRIM27P143735EBI-741158,EBI-719493
TRIM54Q9BYV25EBI-741158,EBI-2130429
TRIP13Q156453EBI-741158,EBI-358993
TSC22D1Q157143EBI-741158,EBI-712609
VAC14Q08AM63EBI-741158,EBI-2107455
XIAPP981703EBI-741158,EBI-517127
ZBTB8AQ96BR93EBI-741158,EBI-742740
ZNF341Q9BYN73EBI-741158,EBI-9089622

Protein-protein interaction databases

BioGridi120405. 132 interactors.
IntActiQ96HA8. 194 interactors.
MINTiMINT-1443224.
STRINGi9606.ENSP00000287387.

Structurei

Secondary structure

1205
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 20Combined sources3
Helixi28 – 41Combined sources14
Beta strandi42 – 45Combined sources4
Helixi47 – 49Combined sources3
Beta strandi50 – 56Combined sources7
Beta strandi62 – 67Combined sources6
Beta strandi76 – 79Combined sources4
Beta strandi81 – 88Combined sources8
Turni89 – 91Combined sources3
Beta strandi92 – 96Combined sources5
Beta strandi100 – 102Combined sources3
Beta strandi104 – 107Combined sources4
Helixi108 – 114Combined sources7
Helixi124 – 126Combined sources3
Beta strandi129 – 134Combined sources6
Helixi135 – 141Combined sources7
Helixi147 – 149Combined sources3
Beta strandi152 – 154Combined sources3
Beta strandi156 – 158Combined sources3
Helixi176 – 179Combined sources4
Beta strandi185 – 192Combined sources8
Helixi193 – 200Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C9QX-ray1.50A2-205[»]
4W79X-ray1.50A2-202[»]
ProteinModelPortaliQ96HA8.
SMRiQ96HA8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96HA8.

Family & Domainsi

Sequence similaritiesi

Belongs to the NTAQ1 family.Curated

Phylogenomic databases

eggNOGiKOG3261. Eukaryota.
ENOG4111G3S. LUCA.
GeneTreeiENSGT00390000014398.
HOGENOMiHOG000007890.
InParanoidiQ96HA8.
OMAiDRRHMKD.
OrthoDBiEOG091G0K1H.
PhylomeDBiQ96HA8.
TreeFamiTF105807.

Family and domain databases

Gene3Di3.10.620.10. 1 hit.
InterProiIPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view]
PfamiPF09764. Nt_Gln_amidase. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96HA8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGNGPAAVH YQPASPPRDA CVYSSCYCEE NIWKLCEYIK NHDQYPLEEC
60 70 80 90 100
YAVFISNERK MIPIWKQQAR PGDGPVIWDY HVVLLHVSSG GQNFIYDLDT
110 120 130 140 150
VLPFPCLFDT YVEDAFKSDD DIHPQFRRKF RVIRADSYLK NFASDRSHMK
160 170 180 190 200
DSSGNWREPP PPYPCIETGD SKMNLNDFIS MDPKVGWGAV YTLSEFTHRF

GSKNC
Length:205
Mass (Da):23,680
Last modified:March 6, 2007 - v2
Checksum:iF858AB0C73928CA9
GO
Isoform 2 (identifier: Q96HA8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.

Note: No experimental confirmation available.
Show »
Length:145
Mass (Da):16,736
Checksum:iAC61BBE11D3642F1
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03088232I → V.1 PublicationCorresponds to variant rs6999234dbSNPEnsembl.1
Natural variantiVAR_03088393N → S.1 PublicationCorresponds to variant rs7014678dbSNPEnsembl.1
Natural variantiVAR_030884116F → I.1 PublicationCorresponds to variant rs6470147dbSNPEnsembl.1
Natural variantiVAR_030885134R → C.1 PublicationCorresponds to variant rs3824250dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0552681 – 60Missing in isoform 2. 1 PublicationAdd BLAST60

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001066 mRNA. Translation: BAA91488.1.
AK293492 mRNA. Translation: BAG56979.1.
AC021305 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW92038.1.
BC008781 mRNA. Translation: AAH08781.1.
CCDSiCCDS6344.1. [Q96HA8-1]
CCDS64965.1. [Q96HA8-2]
RefSeqiNP_001269953.1. NM_001283024.1. [Q96HA8-2]
NP_060494.1. NM_018024.2. [Q96HA8-1]
UniGeneiHs.18029.

Genome annotation databases

EnsembliENST00000287387; ENSP00000287387; ENSG00000156795. [Q96HA8-1]
ENST00000523984; ENSP00000430427; ENSG00000156795. [Q96HA8-2]
GeneIDi55093.
KEGGihsa:55093.
UCSCiuc003yqn.3. human. [Q96HA8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001066 mRNA. Translation: BAA91488.1.
AK293492 mRNA. Translation: BAG56979.1.
AC021305 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW92038.1.
BC008781 mRNA. Translation: AAH08781.1.
CCDSiCCDS6344.1. [Q96HA8-1]
CCDS64965.1. [Q96HA8-2]
RefSeqiNP_001269953.1. NM_001283024.1. [Q96HA8-2]
NP_060494.1. NM_018024.2. [Q96HA8-1]
UniGeneiHs.18029.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C9QX-ray1.50A2-205[»]
4W79X-ray1.50A2-202[»]
ProteinModelPortaliQ96HA8.
SMRiQ96HA8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120405. 132 interactors.
IntActiQ96HA8. 194 interactors.
MINTiMINT-1443224.
STRINGi9606.ENSP00000287387.

PTM databases

iPTMnetiQ96HA8.
PhosphoSitePlusiQ96HA8.

Polymorphism and mutation databases

BioMutaiWDYHV1.
DMDMi152112225.

Proteomic databases

EPDiQ96HA8.
MaxQBiQ96HA8.
PaxDbiQ96HA8.
PeptideAtlasiQ96HA8.
PRIDEiQ96HA8.

Protocols and materials databases

DNASUi55093.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000287387; ENSP00000287387; ENSG00000156795. [Q96HA8-1]
ENST00000523984; ENSP00000430427; ENSG00000156795. [Q96HA8-2]
GeneIDi55093.
KEGGihsa:55093.
UCSCiuc003yqn.3. human. [Q96HA8-1]

Organism-specific databases

CTDi55093.
GeneCardsiWDYHV1.
HGNCiHGNC:25490. WDYHV1.
HPAiHPA024823.
HPA053680.
neXtProtiNX_Q96HA8.
OpenTargetsiENSG00000156795.
PharmGKBiPA164727566.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3261. Eukaryota.
ENOG4111G3S. LUCA.
GeneTreeiENSGT00390000014398.
HOGENOMiHOG000007890.
InParanoidiQ96HA8.
OMAiDRRHMKD.
OrthoDBiEOG091G0K1H.
PhylomeDBiQ96HA8.
TreeFamiTF105807.

Miscellaneous databases

EvolutionaryTraceiQ96HA8.
GeneWikiiC8orf32.
GenomeRNAii55093.
PROiQ96HA8.

Gene expression databases

BgeeiENSG00000156795.
CleanExiHS_WDYHV1.
ExpressionAtlasiQ96HA8. baseline and differential.
GenevisibleiQ96HA8. HS.

Family and domain databases

Gene3Di3.10.620.10. 1 hit.
InterProiIPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view]
PfamiPF09764. Nt_Gln_amidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTAQ1_HUMAN
AccessioniPrimary (citable) accession number: Q96HA8
Secondary accession number(s): B4DE68, Q9NW95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: November 30, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.