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Q96HA8

- NTAQ1_HUMAN

UniProt

Q96HA8 - NTAQ1_HUMAN

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Protein
Protein N-terminal glutamine amidohydrolase
Gene
WDYHV1, C8orf32, NTAQ1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281 By similarity
Active sitei81 – 811 By similarity
Active sitei97 – 971 By similarity

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein-N-terminal glutamine amidohydrolase activity Source: Ensembl

GO - Biological processi

  1. cellular protein modification process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-terminal glutamine amidohydrolase (EC:3.5.1.-)
Alternative name(s):
Protein NH2-terminal glutamine deamidase
Short name:
N-terminal Gln amidase
Short name:
Nt(Q)-amidase
WDYHV motif-containing protein 1
Gene namesi
Name:WDYHV1
Synonyms:C8orf32, NTAQ1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:25490. WDYHV1.

Subcellular locationi

Cytoplasmcytosol By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164727566.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Protein N-terminal glutamine amidohydrolase
PRO_0000279409Add
BLAST

Proteomic databases

MaxQBiQ96HA8.
PaxDbiQ96HA8.
PRIDEiQ96HA8.

PTM databases

PhosphoSiteiQ96HA8.

Expressioni

Gene expression databases

ArrayExpressiQ96HA8.
BgeeiQ96HA8.
CleanExiHS_WDYHV1.
GenevestigatoriQ96HA8.

Organism-specific databases

HPAiHPA024823.

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HPRT1P004923EBI-741158,EBI-748210
HSD17B14Q9BPX13EBI-741158,EBI-742664
JUPP149233EBI-741158,EBI-702484
NME1P155313EBI-741158,EBI-741141
RABAC1Q9UI143EBI-741158,EBI-712367
TRIP13Q156453EBI-741158,EBI-358993

Protein-protein interaction databases

BioGridi120405. 19 interactions.
IntActiQ96HA8. 19 interactions.
MINTiMINT-1443224.
STRINGi9606.ENSP00000287387.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 203
Helixi28 – 4114
Beta strandi42 – 454
Helixi47 – 493
Beta strandi50 – 567
Beta strandi62 – 676
Beta strandi76 – 794
Beta strandi81 – 888
Turni89 – 913
Beta strandi92 – 965
Beta strandi100 – 1023
Beta strandi104 – 1074
Helixi108 – 1147
Helixi124 – 1263
Beta strandi129 – 1346
Helixi135 – 1417
Helixi147 – 1493
Beta strandi152 – 1543
Beta strandi156 – 1583
Helixi176 – 1794
Beta strandi185 – 1928
Helixi193 – 2008

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C9QX-ray1.50A2-205[»]
ProteinModelPortaliQ96HA8.
SMRiQ96HA8. Positions 8-202.

Miscellaneous databases

EvolutionaryTraceiQ96HA8.

Family & Domainsi

Sequence similaritiesi

Belongs to the NTAQ1 family.

Phylogenomic databases

eggNOGiNOG282583.
HOGENOMiHOG000007890.
InParanoidiQ96HA8.
OMAiNSCYCEE.
OrthoDBiEOG70PC0N.
PhylomeDBiQ96HA8.
TreeFamiTF105807.

Family and domain databases

Gene3Di3.10.620.10. 1 hit.
InterProiIPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view]
PfamiPF09764. Nt_Gln_amidase. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96HA8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEGNGPAAVH YQPASPPRDA CVYSSCYCEE NIWKLCEYIK NHDQYPLEEC    50
YAVFISNERK MIPIWKQQAR PGDGPVIWDY HVVLLHVSSG GQNFIYDLDT 100
VLPFPCLFDT YVEDAFKSDD DIHPQFRRKF RVIRADSYLK NFASDRSHMK 150
DSSGNWREPP PPYPCIETGD SKMNLNDFIS MDPKVGWGAV YTLSEFTHRF 200
GSKNC 205
Length:205
Mass (Da):23,680
Last modified:March 6, 2007 - v2
Checksum:iF858AB0C73928CA9
GO
Isoform 2 (identifier: Q96HA8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.

Note: No experimental confirmation available.

Show »
Length:145
Mass (Da):16,736
Checksum:iAC61BBE11D3642F1
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti32 – 321I → V.1 Publication
Corresponds to variant rs6999234 [ dbSNP | Ensembl ].
VAR_030882
Natural varianti93 – 931N → S.1 Publication
Corresponds to variant rs7014678 [ dbSNP | Ensembl ].
VAR_030883
Natural varianti116 – 1161F → I.1 Publication
Corresponds to variant rs6470147 [ dbSNP | Ensembl ].
VAR_030884
Natural varianti134 – 1341R → C.1 Publication
Corresponds to variant rs3824250 [ dbSNP | Ensembl ].
VAR_030885

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6060Missing in isoform 2.
VSP_055268Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK001066 mRNA. Translation: BAA91488.1.
AK293492 mRNA. Translation: BAG56979.1.
AC021305 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW92038.1.
BC008781 mRNA. Translation: AAH08781.1.
CCDSiCCDS6344.1. [Q96HA8-1]
RefSeqiNP_001269953.1. NM_001283024.1.
NP_060494.1. NM_018024.2.
UniGeneiHs.18029.

Genome annotation databases

EnsembliENST00000287387; ENSP00000287387; ENSG00000156795.
ENST00000523984; ENSP00000430427; ENSG00000156795.
GeneIDi55093.
KEGGihsa:55093.
UCSCiuc003yqn.1. human. [Q96HA8-1]

Polymorphism databases

DMDMi152112225.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK001066 mRNA. Translation: BAA91488.1 .
AK293492 mRNA. Translation: BAG56979.1 .
AC021305 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW92038.1 .
BC008781 mRNA. Translation: AAH08781.1 .
CCDSi CCDS6344.1. [Q96HA8-1 ]
RefSeqi NP_001269953.1. NM_001283024.1.
NP_060494.1. NM_018024.2.
UniGenei Hs.18029.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3C9Q X-ray 1.50 A 2-205 [» ]
ProteinModelPortali Q96HA8.
SMRi Q96HA8. Positions 8-202.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120405. 19 interactions.
IntActi Q96HA8. 19 interactions.
MINTi MINT-1443224.
STRINGi 9606.ENSP00000287387.

PTM databases

PhosphoSitei Q96HA8.

Polymorphism databases

DMDMi 152112225.

Proteomic databases

MaxQBi Q96HA8.
PaxDbi Q96HA8.
PRIDEi Q96HA8.

Protocols and materials databases

DNASUi 55093.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000287387 ; ENSP00000287387 ; ENSG00000156795 .
ENST00000523984 ; ENSP00000430427 ; ENSG00000156795 .
GeneIDi 55093.
KEGGi hsa:55093.
UCSCi uc003yqn.1. human. [Q96HA8-1 ]

Organism-specific databases

CTDi 55093.
GeneCardsi GC08P124428.
HGNCi HGNC:25490. WDYHV1.
HPAi HPA024823.
neXtProti NX_Q96HA8.
PharmGKBi PA164727566.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG282583.
HOGENOMi HOG000007890.
InParanoidi Q96HA8.
OMAi NSCYCEE.
OrthoDBi EOG70PC0N.
PhylomeDBi Q96HA8.
TreeFami TF105807.

Miscellaneous databases

EvolutionaryTracei Q96HA8.
GeneWikii C8orf32.
GenomeRNAii 55093.
NextBioi 35471129.
PROi Q96HA8.

Gene expression databases

ArrayExpressi Q96HA8.
Bgeei Q96HA8.
CleanExi HS_WDYHV1.
Genevestigatori Q96HA8.

Family and domain databases

Gene3Di 3.10.620.10. 1 hit.
InterProi IPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view ]
Pfami PF09764. Nt_Gln_amidase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Cerebellum and Embryo.
  2. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-32; SER-93; ILE-116 AND CYS-134.
    Tissue: Uterus.
  5. "Crystal structure of the uncharacterized human protein c8orf32 with bound peptide."
    Center for eukaryotic structural genomics (CESG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 2-205 IN COMPLEX WITH SER-THR-ALA TRIPEPTIDE, SUBUNIT.

Entry informationi

Entry nameiNTAQ1_HUMAN
AccessioniPrimary (citable) accession number: Q96HA8
Secondary accession number(s): B4DE68, Q9NW95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: September 3, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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