Reviewed, UniProtKB/Swiss-Prot Q96GX9 (APIP_HUMAN)

Names and origin

Protein names

Recommended name:
    APAF1-interacting protein

Gene names

Name:	APIP
ORF Names:	CGI-29

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has an anti-apoptotic function and prevents muscle ischemic damage. Inhibits the cytochrome c-dependent and APAF1-mediated cell death. Ref.3
Cofactor	Binds 1 zinc ion per subunit Potential.
Subunit structure	Interacts with APAF1. Ref.3
Subcellular location	Cytoplasm.
Tissue specificity	Ubiquitously expressed with high expression in skeletal muscle, heart and kidney. Ref.3
Sequence similarities	Belongs to the aldolase class II family. APIP subfamily.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	Metal-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-359248,EBI-359248
NUDT18	Q6ZVK8	2	EBI-359248,EBI-740486

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	APAF1-interacting protein		PRO_0000239022
Sites
	Metal binding	115	1	Zinc By similarity
	Metal binding	117	1	Zinc By similarity
Natural variations
	Natural variant	7	1	R → W: dbSNP rs2956114. Ref.2		VAR_026575
	Natural variant	23	1	H → R: dbSNP rs17850326. Ref.2		VAR_026576
	Natural variant	76	1	C → Y: dbSNP rs1977420. Ref.2		VAR_026577
	Natural variant	181	1	M → V: dbSNP rs17850327. Ref.2		VAR_026578
Experimental info
	Sequence conflict	7	1	R → G in AAD27738. Ref.1
	Sequence conflict	172	1	D → T in AAD27738. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96GX9-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 9B8D5D1435D6775A	FASTA	242	27,125
	10 20 30 40 50 60 MSGCDAREGD CCSRRCGAQD KEHPRYLIPE LCKQFYHLGW VTGTGGGISL KHGDEIYIAP 70 80 90 100 110 120 SGVQKERIQP EDMFVCDINE KDISGPSPSK KLKKSQCTPL FMNAYTMRGA GAVIHTHSKA 130 140 150 160 170 180 AVMATLLFPG REFKITHQEM IKGIKKCTSG GYYRYDDMLV VPIIENTPEE KDLKDRMAHA 190 200 210 220 230 240 MNEYPDSCAV LVRRHGVYVW GETWEKAKTM CECYDYLFDI AVSMKKVGLD PSQLPVGENG IV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed: 10810093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS TRP-7; ARG-23; TYR-76 AND VAL-181. Tissue: Bone marrow, Colon and Eye.
[3]	"Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein." Cho D.-H., Hong Y.-M., Lee H.-J., Woo H.-N., Pyo J.-O., Mak T.W., Jung Y.-K. J. Biol. Chem. 279:39942-39950(2004) [PubMed: 15262985] [Abstract] Cited for: FUNCTION, INTERACTION WITH APAF1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[4]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF132963 mRNA. Translation: AAD27738.1. BC008440 mRNA. Translation: AAH08440.1. BC009077 mRNA. Translation: AAH09077.1. BC017594 mRNA. Translation: AAH17594.1.
IPI	IPI00449162. IPI00549730.
RefSeq	NP_057041.2.
UniGene	Hs.447794
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96GX9. 8 interactions.
STRING	Q96GX9.
PTM databases
PhosphoSite	Q96GX9.
Proteomic databases
PeptideAtlas	Q96GX9.
PRIDE	Q96GX9.
Genome annotation databases
Ensembl	ENST00000278359; ENSP00000278359; ENSG00000149089; Homo sapiens. [Genome view]
GeneID	51074.
KEGG	hsa:51074.
UCSC	uc001mvs.2. human. uc009ykg.1. human.
Organism-specific databases
CTD	51074.
GeneCards	GC11M034861.
H-InvDB	HIX0009553.
HGNC	HGNC:17581. APIP.
MIM	612491. gene.
PharmGKB	PA142672601.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96GX9.
HOVERGEN	Q96GX9.
OrthoDB	EOG9V9X9G
Gene expression databases
ArrayExpress	Q96GX9.
Bgee	Q96GX9.
CleanEx	HS_APIP.
Genevestigator	Q96GX9.
GermOnline	ENSG00000149089. Homo sapiens.
Family and domain databases
InterPro	IPR001303. Aldolase_II/adducin_N. IPR017714. MethylthioRu-1-P_deHdtase_MtnB. [Graphical view]
Gene3D	G3DSA:3.40.225.10. Aldolase_II/adducin_N. 1 hit.
Pfam	PF00596. Aldolase_II. 1 hit. [Graphical view]
TIGRFAMs	TIGR03328. salvage_mtnB. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	53699.
SOURCE	Search...

Entry information

Entry name

APIP_HUMAN

Accession

Primary (citable) accession number: Q96GX9
Secondary accession number(s): Q6PJX6 , Q8WVU2, Q96HK2, Q9Y318

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	December 1, 2001
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.