Q96GW9 (SYMM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase, mitochondrial
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase 2
Mitochondrial methionyl-tRNA synthetase
Short name=MtMetRS

Gene names

Name:

MARS2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). Ref.5
Subcellular location	Mitochondrion matrix Probable.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Biophysicochemical properties	Kinetic parameters: KM=18 µM for Met Ref.5 KM=85 µM for ATP KM=2.1 µM for tRNA-Met pH dependence: Optimum pH is 7.8-8.2.
Sequence caution	The sequence AAH09115.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH40934.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from direct assay Ref.5. Source: UniProtKB
Cellular component	mitochondrial matrix Inferred from direct assay Ref.5. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 29	29	Mitochondrion Potential
	Chain	30 – 593	564	Methionine--tRNA ligase, mitochondrial		PRO_0000045493
Regions
	Motif	52 – 62	11	"HIGH" region
	Motif	347 – 351	5	"KMSKS" region
Sites
	Binding site	350	1	ATP By similarity
Experimental info
	Sequence conflict	535 – 536	2	LL → MM in BAC92749. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96GW9 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: A5076FD9F31F4B96	FASTA	593	66,591
	10 20 30 40 50 60 MLRTSVLRLL GRTGASRLSL LEDFGPRYYS SGSLSAGDDA CDVRAYFTTP IFYVNAAPHI 70 80 90 100 110 120 GHLYSALLAD ALCRHRRLRG PSTAATRFST GTDEHGLKIQ QAAATAGLAP TELCDRVSEQ 130 140 150 160 170 180 FQQLFQEAGI SCTDFIRTTE ARHRVAVQHF WGVLKSRGLL YKGVYEGWYC ASDECFLPEA 190 200 210 220 230 240 KVTQQPGPSG DSFPVSLESG HPVSWTKEEN YIFRLSQFRK PLQRWLRGNP QAITPEPFHH 250 260 270 280 290 300 VVLQWLDEEL PDLSVSRRSS HLHWGIPVPG DDSQTIYVWL DALVNYLTVI GYPNAEFKSW 310 320 330 340 350 360 WPATSHIIGK DILKFHAIYW PAFLLGAGMS PPQRICVHSH WTVCGQKMSK SLGNVVDPRT 370 380 390 400 410 420 CLNRYTVDGF RYFLLRQGVP NWDCDYYDEK VVKLLNSELA DALGGLLNRC TAKRINPSET 430 440 450 460 470 480 YPAFCTTCFP SEPGLVGPSV RAQAEDYALV SAVATLPKQV ADHYDNFRIY KALEAVSSCV 490 500 510 520 530 540 RQTNGFVQRH APWKLNWESP VDAPWLGTVL HVALECLRVF GTLLQPVTPS LADKLLSRLG 550 560 570 580 590 VSASERSLGE LYFLPRFYGH PCPFEGRRLG PETGLLFPRL DQSRTWLVKA HRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Downregulation of mitochondrial translation factors during the differentiation of HL-60 cells by TPA." Murata H., Takeuchi-Tomita N. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Trachea.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Colon.
[5]	"Characterization of the human mitochondrial methionyl-tRNA synthetase." Spencer A.C., Heck A., Takeuchi N., Watanabe K., Spremulli L.L. Biochemistry 43:9743-9754(2004) [PubMed: 15274629] [Abstract] Cited for: ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB107013 mRNA. Translation: BAC92749.1. AK098121 mRNA. Translation: BAC05238.1. AC073058 Genomic DNA. Translation: AAX93244.1. BC009115 mRNA. Translation: AAH09115.1. Different initiation. BC040934 mRNA. Translation: AAH40934.1. Different initiation. BC126294 mRNA. Translation: AAI26295.1.
IPI	IPI00062839.
RefSeq	NP_612404.1. NM_138395.3.
UniGene	Hs.116602.
3D structure databases
ProteinModelPortal	Q96GW9.
SMR	Q96GW9. Positions 302-361.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96GW9.
Polymorphism databases
DMDM	85541638.
Proteomic databases
PRIDE	Q96GW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000282276; ENSP00000282276; ENSG00000152428.
GeneID	92935.
KEGG	hsa:92935.
NMPDR	fig|9606.3.peg.19100.
UCSC	uc002uuq.1. human.
Organism-specific databases
CTD	92935.
GeneCards	GC02P198570.
H-InvDB	HIX0002712.
HGNC	HGNC:25133. MARS2.
HPA	HPA035589.
MIM	609728. gene.
neXtProt	NX_Q96GW9.
PharmGKB	PA134863396.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19743.
GeneTree	ENSGT00550000075136.
HOGENOM	HBG721667.
HOVERGEN	HBG080702.
InParanoid	Q96GW9.
OMA	FWGVLKS.
OrthoDB	EOG4T7830.
PhylomeDB	Q96GW9.
Enzyme and pathway databases
Reactome	REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q96GW9.
Bgee	Q96GW9.
CleanEx	HS_MARS2.
Genevestigator	Q96GW9.
GermOnline	ENSG00000152428. Homo sapiens.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR014758. Met-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00134. L-Methionine.
NextBio	77920.
SOURCE	Search...

Entry information

Entry name

SYMM_HUMAN

Accession

Primary (citable) accession number: Q96GW9
Secondary accession number(s): A0AVC3 , Q76E79, Q8IW62, Q8N7N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	January 10, 2006
Last modified:	January 25, 2012
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.