Q96GW7 (PGCB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Brevican core protein

Alternative name(s):
Brain-enriched hyaluronan-binding protein
Short name=BEHAB
Chondroitin sulfate proteoglycan 7

Gene names

Name:	BCAN
Synonyms:	BEHAB, CSPG7
ORF Names:	UNQ2525/PRO6018

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.
Subunit structure	Interacts with TNR By similarity.
Subcellular location	Isoform 1: Secreted › extracellular space › extracellular matrix. Isoform 2: Membrane; Lipid-anchor › GPI-anchor.
Developmental stage	Isoform 1 is highly expressed from birth through 8 years of age and is down-regulated by 20 years of age to low levels that are maintained in the normal adult cortex. Isoform 2 is expressed at uniformly low levels throughout development. Ref.1
Post-translational modification	Contains mostly chondroitin sulfate By similarity. O-glycosylated with a core 1 or possibly core 8 glycan. Ref.6
Sequence similarities	Belongs to the aggrecan/versican proteoglycan family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 2 Link domains. Contains 1 Sushi (CCP/SCR) domain.

Ontologies

Keywords
Cellular component	Extracellular matrix Membrane Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	EGF-like domain Immunoglobulin domain Repeat Signal Sushi
Ligand	Hyaluronic acid Lectin
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein Proteoglycan
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyaluronic acid binding Inferred from electronic annotation. Source: UniProtKB-KW sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96GW7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96GW7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     649-671: DCVPSPCHNGGTCLEEEEGVRCL → NSAQGSTALSILLLFFPLQLWVT
     672-911: Missing.

Note: GPI-anchor amidated asparagine on Asn-649 (Potential).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 911	889	Brevican core protein		PRO_0000017511
Regions
	Domain	36 – 155	120	Ig-like V-type
	Domain	157 – 252	96	Link 1
	Domain	257 – 354	98	Link 2
	Domain	646 – 682	37	EGF-like
	Domain	695 – 809	115	C-type lectin
	Domain	813 – 873	61	Sushi
	Region	520 – 530	11	O-glycosylated at two sites
	Region	540 – 545	6	O-glycosylated at two sites
	Region	569 – 575	7	O-glycosylated at one site
	Compositional bias	385 – 477	93	Glu-rich
Amino acid modifications
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	337	1	N-linked (GlcNAc...) Potential
	Glycosylation	905	1	O-linked (GalNAc...) Probable
	Glycosylation	906	1	O-linked (GalNAc...) Probable
	Disulfide bond	57 ↔ 137		By similarity
	Disulfide bond	179 ↔ 250		By similarity
	Disulfide bond	203 ↔ 224		By similarity
	Disulfide bond	277 ↔ 352		By similarity
	Disulfide bond	301 ↔ 322		By similarity
	Disulfide bond	650 ↔ 661		By similarity
	Disulfide bond	655 ↔ 670		By similarity
	Disulfide bond	672 ↔ 681		By similarity
	Disulfide bond	688 ↔ 699		By similarity
	Disulfide bond	716 ↔ 808		By similarity
	Disulfide bond	784 ↔ 800		By similarity
	Disulfide bond	815 ↔ 858		By similarity
	Disulfide bond	844 ↔ 871		By similarity
Natural variations
	Alternative sequence	649 – 671	23	DCVPS…GVRCL → NSAQGSTALSILLLFFPLQL WVT in isoform 2.		VSP_011184
	Alternative sequence	672 – 911	240	Missing in isoform 2.		VSP_011185
	Natural variant	356	1	S → L. Corresponds to variant rs12065791 [ dbSNP | Ensembl ].		VAR_050123
	Natural variant	504	1	E → K. Ref.2 Corresponds to variant rs1056695 [ dbSNP | Ensembl ].		VAR_019551
Experimental info
	Sequence conflict	175	1	A → T in AAH22938. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 5268E796FA3938D2	FASTA	911	99,118
	10 20 30 40 50 60 MAQLFLPLLA ALVLAQAPAA LADVLEGDSS EDRAFRVRIA GDAPLQGVLG GALTIPCHVH 70 80 90 100 110 120 YLRPPPSRRA VLGSPRVKWT FLSRGREAEV LVARGVRVKV NEAYRFRVAL PAYPASLTDV 130 140 150 160 170 180 SLALSELRPN DSGIYRCEVQ HGIDDSSDAV EVKVKGVVFL YREGSARYAF SFSGAQEACA 190 200 210 220 230 240 RIGAHIATPE QLYAAYLGGY EQCDAGWLSD QTVRYPIQTP REACYGDMDG FPGVRNYGVV 250 260 270 280 290 300 DPDDLYDVYC YAEDLNGELF LGDPPEKLTL EEARAYCQER GAEIATTGQL YAAWDGGLDH 310 320 330 340 350 360 CSPGWLADGS VRYPIVTPSQ RCGGGLPGVK TLFLFPNQTG FPNKHSRFNV YCFRDSAQPS 370 380 390 400 410 420 AIPEASNPAS NPASDGLEAI VTVTETLEEL QLPQEATESE SRGAIYSIPI MEDGGGGSST 430 440 450 460 470 480 PEDPAEAPRT LLEFETQSMV PPTGFSEEEG KALEEEEKYE DEEEKEEEEE EEEVEDEALW 490 500 510 520 530 540 AWPSELSSPG PEASLPTEPA AQEESLSQAP ARAVLQPGAS PLPDGESEAS RPPRVHGPPT 550 560 570 580 590 600 ETLPTPRERN LASPSPSTLV EAREVGEATG GPELSGVPRG ESEETGSSEG APSLLPATRA 610 620 630 640 650 660 PEGTRELEAP SEDNSGRTAP AGTSVQAQPV LPTDSASRGG VAVVPASGDC VPSPCHNGGT 670 680 690 700 710 720 CLEEEEGVRC LCLPGYGGDL CDVGLRFCNP GWDAFQGACY KHFSTRRSWE EAETQCRMYG 730 740 750 760 770 780 AHLASISTPE EQDFINNRYR EYQWIGLNDR TIEGDFLWSD GVPLLYENWN PGQPDSYFLS 790 800 810 820 830 840 GENCVVMVWH DQGQWSDVPC NYHLSYTCKM GLVSCGPPPE LPLAQVFGRP RLRYEVDTVL 850 860 870 880 890 900 RYRCREGLAQ RNLPLIRCQE NGRWEAPQIS CVPRRPARAL HPEEDPEGRQ GRLLGRWKAL 910 LIPPSSPMPG P
	Isoform 2 [UniParc]. Checksum: BAD0E1959546D4B9	FASTA	671	71,671
	10 20 30 40 50 60 MAQLFLPLLA ALVLAQAPAA LADVLEGDSS EDRAFRVRIA GDAPLQGVLG GALTIPCHVH 70 80 90 100 110 120 YLRPPPSRRA VLGSPRVKWT FLSRGREAEV LVARGVRVKV NEAYRFRVAL PAYPASLTDV 130 140 150 160 170 180 SLALSELRPN DSGIYRCEVQ HGIDDSSDAV EVKVKGVVFL YREGSARYAF SFSGAQEACA 190 200 210 220 230 240 RIGAHIATPE QLYAAYLGGY EQCDAGWLSD QTVRYPIQTP REACYGDMDG FPGVRNYGVV 250 260 270 280 290 300 DPDDLYDVYC YAEDLNGELF LGDPPEKLTL EEARAYCQER GAEIATTGQL YAAWDGGLDH 310 320 330 340 350 360 CSPGWLADGS VRYPIVTPSQ RCGGGLPGVK TLFLFPNQTG FPNKHSRFNV YCFRDSAQPS 370 380 390 400 410 420 AIPEASNPAS NPASDGLEAI VTVTETLEEL QLPQEATESE SRGAIYSIPI MEDGGGGSST 430 440 450 460 470 480 PEDPAEAPRT LLEFETQSMV PPTGFSEEEG KALEEEEKYE DEEEKEEEEE EEEVEDEALW 490 500 510 520 530 540 AWPSELSSPG PEASLPTEPA AQEESLSQAP ARAVLQPGAS PLPDGESEAS RPPRVHGPPT 550 560 570 580 590 600 ETLPTPRERN LASPSPSTLV EAREVGEATG GPELSGVPRG ESEETGSSEG APSLLPATRA 610 620 630 640 650 660 PEGTRELEAP SEDNSGRTAP AGTSVQAQPV LPTDSASRGG VAVVPASGNS AQGSTALSIL 670 LLFFPLQLWV T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning, chromosomal localization, and expression analysis of human BEHAB/brevican, a brain specific proteoglycan regulated during cortical development and in glioma." Gary S.C., Zerillo C.A., Chiang V.L., Gaw J.U., Gray G., Hockfield S. Gene 256:139-147(2000) [PubMed: 11054543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), DEVELOPMENTAL STAGE. Tissue: Brain cortex.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LYS-504.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[6]	"Enrichment of glycopeptides for glycan structure and attachment site identification." Nilsson J., Rueetschi U., Halim A., Hesse C., Carlsohn E., Brinkmalm G., Larson G. Nat. Methods 6:809-811(2009) [PubMed: 19838169] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT SER-905 AND SER-906, STRUCTURE OF CARBOHYDRATES, MASS SPECTROMETRY. Tissue: Cerebrospinal fluid.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Brevican

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF228710 mRNA. Translation: AAG23134.1. AF229053 mRNA. Translation: AAG23135.1. AY358372 mRNA. Translation: AAQ88738.1. AL365181, AL590666 Genomic DNA. Translation: CAI13056.1. AL365181 Genomic DNA. Translation: CAI13060.1. AL590666, AL365181 Genomic DNA. Translation: CAI16352.1. CH471121 Genomic DNA. Translation: EAW52927.1. CH471121 Genomic DNA. Translation: EAW52928.1. CH471121 Genomic DNA. Translation: EAW52929.1. CH471121 Genomic DNA. Translation: EAW52930.1. BC009117 mRNA. Translation: AAH09117.1. BC022938 mRNA. Translation: AAH22938.1. BC027971 mRNA. Translation: AAH27971.1.
IPI	IPI00456623. IPI00456624.
RefSeq	NP_068767.3. NM_021948.4. NP_940819.1. NM_198427.1.
UniGene	Hs.516904. Hs.663621.
3D structure databases
ProteinModelPortal	Q96GW7.
SMR	Q96GW7. Positions 43-156, 158-256, 259-357, 649-872.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96GW7. 1 interaction.
MINT	MINT-4830140.
STRING	Q96GW7.
PTM databases
PhosphoSite	Q96GW7.
Polymorphism databases
DMDM	68067899.
Proteomic databases
PeptideAtlas	Q96GW7.
PRIDE	Q96GW7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000329117; ENSP00000331210; ENSG00000132692.
GeneID	63827.
KEGG	hsa:63827.
UCSC	uc001fpo.1. human. uc001fpp.1. human.
Organism-specific databases
CTD	63827.
GeneCards	GC01P156611.
H-InvDB	HIX0001168.
HGNC	HGNC:23059. BCAN.
HPA	CAB025862. HPA007865.
MIM	600347. gene.
neXtProt	NX_Q96GW7.
PharmGKB	PA134868393.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG716672.
HOVERGEN	HBG008175.
InParanoid	Q96GW7.
OMA	YCFRDSA.
OrthoDB	EOG4XGZZW.
PhylomeDB	Q96GW7.
Gene expression databases
ArrayExpress	Q96GW7.
Bgee	Q96GW7.
CleanEx	HS_BCAN.
Genevestigator	Q96GW7.
GermOnline	ENSG00000132692. Homo sapiens.
Family and domain databases
InterPro	IPR002353. AntifreezeII. IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR018378. C-type_lectin_CS. IPR016187. C-type_lectin_fold. IPR016060. Complement_control_module. IPR006209. EGF. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003006. Ig/MHC_CS. IPR013106. Ig_V-set. IPR003596. Ig_V-set_subgr. IPR000538. Link. IPR000436. Sushi_SCR_CCP. [Graphical view]
Gene3D	G3DSA:3.10.100.10. C-type_lectin-like. 3 hits. G3DSA:2.10.70.10. Complement_control_module. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K06795.
Pfam	PF00008. EGF. 1 hit. PF00059. Lectin_C. 1 hit. PF00084. Sushi. 1 hit. PF07686. V-set. 1 hit. PF00193. Xlink. 2 hits. [Graphical view]
PRINTS	PR00356. ANTIFREEZEII. PR01265. LINKMODULE.
SMART	SM00032. CCP. 1 hit. SM00034. CLECT. 1 hit. SM00181. EGF. 1 hit. SM00406. IGv. 1 hit. SM00445. LINK. 2 hits. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 3 hits. SSF57535. Complement_control_module. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. 1 hit. PS50041. C_TYPE_LECTIN_2. 1 hit. PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS50835. IG_LIKE. 1 hit. PS00290. IG_MHC. 1 hit. PS01241. LINK_1. 2 hits. PS50963. LINK_2. 2 hits. PS50923. SUSHI. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	65542.
SOURCE	Search...

Entry information

Entry name

PGCB_HUMAN

Accession

Primary (citable) accession number: Q96GW7
Secondary accession number(s): D3DVC2 , Q5SZ10, Q5T3I5, Q8TBB9, Q9HBK1, Q9HBK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.