Q96GQ7 (DDX27_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ATP-dependent RNA helicase DDX27
EC=3.6.4.13

Alternative name(s):
DEAD box protein 27

Gene names

Name:	DDX27
Synonyms:	RHLP
ORF Names:	HSPC259, PP3241

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	796 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable ATP-dependent RNA helicase.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Caution	It is uncertain whether Met-1 or Met-32 is the initiator.
Sequence caution	The sequence AAF28937.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAF28937.1 differs from that shown. Reason: Sequencing errors. The sequence AAG22482.1 differs from that shown. Reason: Frameshift at positions 208, 427, 434 and 532. The sequence AAH16060.2 differs from that shown. Reason: Erroneous initiation. The sequence BAA91284.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14343.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 796	796	Probable ATP-dependent RNA helicase DDX27		PRO_0000055031
Regions
	Domain	249 – 423	175	Helicase ATP-binding
	Domain	457 – 603	147	Helicase C-terminal
	Nucleotide binding	262 – 269	8	ATP By similarity
	Motif	195 – 200	6	Nuclear localization signal Potential
	Motif	218 – 246	29	Q motif
	Motif	371 – 374	4	DEAD box
	Compositional bias	44 – 200	157	Asp/Glu/Lys-rich
Amino acid modifications
	Modified residue	54	1	Phosphoserine By similarity
	Modified residue	56	1	Phosphoserine By similarity
	Modified residue	79	1	Phosphoserine Ref.7 Ref.8
	Modified residue	166	1	Phosphoserine Ref.7
	Modified residue	177	1	Phosphoserine Ref.7
Natural variations
	Natural variant	766	1	G → S. Ref.3 Ref.4 Corresponds to variant rs1130146 [ dbSNP | Ensembl ].		VAR_022849
Experimental info
	Sequence conflict	482	1	L → F in AAK21271. Ref.1
	Sequence conflict	542	1	N → S in BAA91284. Ref.4
	Sequence conflict	548	1	V → D in AAK95821. Ref.1
	Sequence conflict	548	1	V → D in BAB14343. Ref.4
	Sequence conflict	674	1	A → T in AAK21271. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96GQ7 [UniParc]. Last modified March 28, 2003. Version 2. Checksum: 9282C712B8F8B84A	FASTA	796	89,835
	10 20 30 40 50 60 MVLAQRRRGG CEKLRAGPQA VLASGSGFCD NMLADLGLIG TIGEDDEVPV EPESDSGDEE 70 80 90 100 110 120 EEGPIVLGRR QKALGKNRSA DFNPDFVFTE KEGTYDGSWA LADVMSQLKK KRAATTLDEK 130 140 150 160 170 180 IEKVRKKRKT EDKEAKSGKL EKEKEAKEGS EPKEQEDLQE NDEEGSEDEA SETDYSSADE 190 200 210 220 230 240 NILTKADTLK VKDRKKKKKK GQEAGGFFED ASQYDENLSF QDMNLSRPLL KAITAMGFKQ 250 260 270 280 290 300 PTPIQKACIP VGLLGKDICA CAATGTGKTA AFALPVLERL IYKPRQAPVT RVLVLVPTRE 310 320 330 340 350 360 LGIQVHSVTR QLAQFCNITT CLAVGGLDVK SQEAALRAAP DILIATPGRL IDHLHNCPSF 370 380 390 400 410 420 HLSSIEVLIL DEADRMLDEY FEEQMKEIIR MCSHHRQTML FSATMTDEVK DLASVSLKNP 430 440 450 460 470 480 VRIFVNSNTD VAPFLRQEFI RIRPNREGDR EAIVAALLTR TFTDHVMLFT QTKKQAHRMH 490 500 510 520 530 540 ILLGLMGLQV GELHGNLSQT QRLEALRRFK DEQIDILVAT DVAARGLDIE GVKTVINFTM 550 560 570 580 590 600 PNTIKHYVHR VGRTARAGRA GRSVSLVGED ERKMLKEIVK AAKAPVKARI LPQDVILKFR 610 620 630 640 650 660 DKIEKMEKDV YAVLQLEAEE KEMQQSEAQI NTAKRLLEKG KEAVVQEPER SWFQTKEERK 670 680 690 700 710 720 KEKIAKALQE FDLALRGKKK RKKFMKDAKK KGEMTAEERS QFEILKAQMF AERLAKRNRR 730 740 750 760 770 780 AKRARAMPEE EPVRGPAKKQ KQGKKSVFDE ELTNTSKKAL KQYRAGPSFE ERKQLGLPHQ 790 RRGGNFKSKS RYKRRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Wang Y., Fan Y.-Z., Han W.-L., Yang T., Gao Y., Ma D.-L. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-766. Tissue: Brain, Muscle and Skin.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 24-796, VARIANT SER-766. Tissue: Stomach cancer and Teratocarcinoma.
[5]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 129-688.
[6]	"Human partial CDS from CD34+ stem cells." Ye M., Zhang Q.-H., Zhou J., Shen Y., Wu X.-Y., Guan Z.Q., Wang L., Fan H.-Y., Mao Y.-F., Dai M., Huang Q.-H., Chen S.-J., Chen Z. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 600-796. Tissue: Umbilical cord blood.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79; SER-166 AND SER-177, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY044431 mRNA. Translation: AAK95821.1. AF336851 mRNA. Translation: AAK21271.1. AL049766, AL357560 Genomic DNA. Translation: CAI22427.1. AL357560, AL049766 Genomic DNA. Translation: CAH70236.1. BC009304 mRNA. Translation: AAH09304.2. BC011927 mRNA. Translation: AAH11927.2. BC016060 mRNA. Translation: AAH16060.2. Different initiation. BC126287 mRNA. Translation: AAI26288.1. BC130275 mRNA. Translation: AAI30276.1. BC144125 mRNA. Translation: AAI44126.1. AK022979 mRNA. Translation: BAB14343.1. Different initiation. AK000603 mRNA. Translation: BAA91284.1. Different initiation. AF193054 mRNA. Translation: AAG22482.1. Frameshift. AF161377 mRNA. Translation: AAF28937.1. Sequence problems.
IPI	IPI00293078.
RefSeq	NP_060365.7. NM_017895.7.
UniGene	Hs.129261. Hs.65234.
3D structure databases
ProteinModelPortal	Q96GQ7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96GQ7. 5 interactions.
MINT	MINT-1420816.
PTM databases
PhosphoSite	Q96GQ7.
Polymorphism databases
DMDM	29427946.
2D gel databases
SWISS-2DPAGE	Q96GQ7.
Proteomic databases
PaxDb	Q96GQ7.
PeptideAtlas	Q96GQ7.
PRIDE	Q96GQ7.
Protocols and materials databases
DNASU	55661.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000371764; ENSP00000360828; ENSG00000124228.
GeneID	55661.
KEGG	hsa:55661.
UCSC	uc002xuh.3. human.
Organism-specific databases
CTD	55661.
GeneCards	GC20P047835.
HGNC	HGNC:15837. DDX27.
HPA	HPA047087.
neXtProt	NX_Q96GQ7.
PharmGKB	PA27213.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0513.
HOVERGEN	HBG106162.
InParanoid	Q96GQ7.
KO	K13181.
OMA	TRTFTDH.
OrthoDB	EOG4NVZJV.
PhylomeDB	Q96GQ7.
Gene expression databases
ArrayExpress	Q96GQ7.
Bgee	Q96GQ7.
CleanEx	HS_DDX27.
Genevestigator	Q96GQ7.
GermOnline	ENSG00000124228. Homo sapiens.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DDX27. human.
GenomeRNAi	55661.
NextBio	60399.

Entry information

Entry name

DDX27_HUMAN

Accession

Primary (citable) accession number: Q96GQ7
Secondary accession number(s): A0AVB6 , B7ZLY1, Q5VXM7, Q8WYG4, Q969N7, Q96F57, Q96L97, Q9BWY9, Q9BXF0, Q9H990, Q9NWU3, Q9P0C2, Q9UGD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 28, 2003
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.