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Q96GQ7 (DDX27_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ATP-dependent RNA helicase DDX27

EC=3.6.4.13
Alternative name(s):
DEAD box protein 27
Gene names
Name:DDX27
Synonyms:RHLP
ORF Names:HSPC259, PP3241
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length796 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable ATP-dependent RNA helicase.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subcellular location

Nucleus Potential.

Sequence similarities

Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Caution

It is uncertain whether Met-1 or Met-32 is the initiator.

Sequence caution

The sequence AAF28937.1 differs from that shown. Reason: Frameshift at several positions.

The sequence AAF28937.1 differs from that shown. Reason: Sequencing errors.

The sequence AAG22482.1 differs from that shown. Reason: Frameshift at positions 208, 427, 434 and 532.

The sequence AAH16060.2 differs from that shown. Reason: Erroneous initiation.

The sequence BAA91284.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB14343.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityPolymorphism
   LigandATP-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

poly(A) RNA binding

Inferred from direct assay PubMed 22658674PubMed 22681889. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 796796Probable ATP-dependent RNA helicase DDX27
PRO_0000055031

Regions

Domain249 – 423175Helicase ATP-binding
Domain457 – 603147Helicase C-terminal
Nucleotide binding262 – 2698ATP By similarity
Motif195 – 2006Nuclear localization signal Potential
Motif218 – 24629Q motif
Motif371 – 3744DEAD box
Compositional bias44 – 200157Asp/Glu/Lys-rich

Amino acid modifications

Modified residue541Phosphoserine By similarity
Modified residue561Phosphoserine By similarity
Modified residue791Phosphoserine Ref.7 Ref.8
Modified residue1661Phosphoserine Ref.7
Modified residue1771Phosphoserine Ref.7

Natural variations

Natural variant7661G → S. Ref.3 Ref.4
Corresponds to variant rs1130146 [ dbSNP | Ensembl ].
VAR_022849

Experimental info

Sequence conflict4821L → F in AAK21271. Ref.1
Sequence conflict5421N → S in BAA91284. Ref.4
Sequence conflict5481V → D in AAK95821. Ref.1
Sequence conflict5481V → D in BAB14343. Ref.4
Sequence conflict6741A → T in AAK21271. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q96GQ7 [UniParc].

Last modified March 28, 2003. Version 2.
Checksum: 9282C712B8F8B84A

FASTA79689,835
        10         20         30         40         50         60 
MVLAQRRRGG CEKLRAGPQA VLASGSGFCD NMLADLGLIG TIGEDDEVPV EPESDSGDEE 

        70         80         90        100        110        120 
EEGPIVLGRR QKALGKNRSA DFNPDFVFTE KEGTYDGSWA LADVMSQLKK KRAATTLDEK 

       130        140        150        160        170        180 
IEKVRKKRKT EDKEAKSGKL EKEKEAKEGS EPKEQEDLQE NDEEGSEDEA SETDYSSADE 

       190        200        210        220        230        240 
NILTKADTLK VKDRKKKKKK GQEAGGFFED ASQYDENLSF QDMNLSRPLL KAITAMGFKQ 

       250        260        270        280        290        300 
PTPIQKACIP VGLLGKDICA CAATGTGKTA AFALPVLERL IYKPRQAPVT RVLVLVPTRE 

       310        320        330        340        350        360 
LGIQVHSVTR QLAQFCNITT CLAVGGLDVK SQEAALRAAP DILIATPGRL IDHLHNCPSF 

       370        380        390        400        410        420 
HLSSIEVLIL DEADRMLDEY FEEQMKEIIR MCSHHRQTML FSATMTDEVK DLASVSLKNP 

       430        440        450        460        470        480 
VRIFVNSNTD VAPFLRQEFI RIRPNREGDR EAIVAALLTR TFTDHVMLFT QTKKQAHRMH 

       490        500        510        520        530        540 
ILLGLMGLQV GELHGNLSQT QRLEALRRFK DEQIDILVAT DVAARGLDIE GVKTVINFTM 

       550        560        570        580        590        600 
PNTIKHYVHR VGRTARAGRA GRSVSLVGED ERKMLKEIVK AAKAPVKARI LPQDVILKFR 

       610        620        630        640        650        660 
DKIEKMEKDV YAVLQLEAEE KEMQQSEAQI NTAKRLLEKG KEAVVQEPER SWFQTKEERK 

       670        680        690        700        710        720 
KEKIAKALQE FDLALRGKKK RKKFMKDAKK KGEMTAEERS QFEILKAQMF AERLAKRNRR 

       730        740        750        760        770        780 
AKRARAMPEE EPVRGPAKKQ KQGKKSVFDE ELTNTSKKAL KQYRAGPSFE ERKQLGLPHQ 

       790 
RRGGNFKSKS RYKRRK 

« Hide

References

« Hide 'large scale' references
[1]Wang Y., Fan Y.-Z., Han W.-L., Yang T., Gao Y., Ma D.-L.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-766.
Tissue: Brain, Muscle and Skin.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 24-796, VARIANT SER-766.
Tissue: Stomach cancer and Teratocarcinoma.
[5]"Large-scale cDNA transfection screening for genes related to cancer development and progression."
Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. expand/collapse author list , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 129-688.
[6]"Human partial CDS from CD34+ stem cells."
Ye M., Zhang Q.-H., Zhou J., Shen Y., Wu X.-Y., Guan Z.Q., Wang L., Fan H.-Y., Mao Y.-F., Dai M., Huang Q.-H., Chen S.-J., Chen Z.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 600-796.
Tissue: Umbilical cord blood.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79; SER-166 AND SER-177, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY044431 mRNA. Translation: AAK95821.1.
AF336851 mRNA. Translation: AAK21271.1.
AL049766, AL357560 Genomic DNA. Translation: CAI22427.1.
AL357560, AL049766 Genomic DNA. Translation: CAH70236.1.
BC009304 mRNA. Translation: AAH09304.2.
BC011927 mRNA. Translation: AAH11927.2.
BC016060 mRNA. Translation: AAH16060.2. Different initiation.
BC126287 mRNA. Translation: AAI26288.1.
BC130275 mRNA. Translation: AAI30276.1.
BC144125 mRNA. Translation: AAI44126.1.
AK022979 mRNA. Translation: BAB14343.1. Different initiation.
AK000603 mRNA. Translation: BAA91284.1. Different initiation.
AF193054 mRNA. Translation: AAG22482.1. Frameshift.
AF161377 mRNA. Translation: AAF28937.1. Sequence problems.
CCDSCCDS13416.1.
RefSeqNP_060365.7. NM_017895.7.
UniGeneHs.129261.
Hs.65234.

3D structure databases

ProteinModelPortalQ96GQ7.
SMRQ96GQ7. Positions 218-581.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120793. 13 interactions.
IntActQ96GQ7. 6 interactions.
MINTMINT-1420816.

PTM databases

PhosphoSiteQ96GQ7.

Polymorphism databases

DMDM29427946.

2D gel databases

SWISS-2DPAGEQ96GQ7.

Proteomic databases

MaxQBQ96GQ7.
PaxDbQ96GQ7.
PeptideAtlasQ96GQ7.
PRIDEQ96GQ7.

Protocols and materials databases

DNASU55661.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000371764; ENSP00000360828; ENSG00000124228.
GeneID55661.
KEGGhsa:55661.
UCSCuc002xuh.3. human.

Organism-specific databases

CTD55661.
GeneCardsGC20P047835.
HGNCHGNC:15837. DDX27.
HPAHPA047087.
neXtProtNX_Q96GQ7.
PharmGKBPA27213.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0513.
HOVERGENHBG106162.
InParanoidQ96GQ7.
KOK13181.
OMAQFCNITT.
OrthoDBEOG7G1V5Q.
PhylomeDBQ96GQ7.
TreeFamTF314780.

Gene expression databases

ArrayExpressQ96GQ7.
BgeeQ96GQ7.
CleanExHS_DDX27.
GenevestigatorQ96GQ7.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDDX27. human.
GeneWikiDDX27.
GenomeRNAi55661.
NextBio60399.
PROQ96GQ7.

Entry information

Entry nameDDX27_HUMAN
AccessionPrimary (citable) accession number: Q96GQ7
Secondary accession number(s): A0AVB6 expand/collapse secondary AC list , B7ZLY1, Q5VXM7, Q8WYG4, Q969N7, Q96F57, Q96L97, Q9BWY9, Q9BXF0, Q9H990, Q9NWU3, Q9P0C2, Q9UGD6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: July 9, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM