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Q96GQ7

- DDX27_HUMAN

UniProt

Q96GQ7 - DDX27_HUMAN

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Protein
Probable ATP-dependent RNA helicase DDX27
Gene
DDX27, RHLP, HSPC259, PP3241
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Probable ATP-dependent RNA helicase.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi262 – 2698ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. poly(A) RNA binding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX27 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 27
Gene namesi
Name:DDX27
Synonyms:RHLP
ORF Names:HSPC259, PP3241
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:15837. DDX27.

Subcellular locationi

Nucleus Reviewed prediction

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27213.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 796796Probable ATP-dependent RNA helicase DDX27
PRO_0000055031Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei54 – 541Phosphoserine By similarity
Modified residuei56 – 561Phosphoserine By similarity
Modified residuei79 – 791Phosphoserine2 Publications
Modified residuei166 – 1661Phosphoserine1 Publication
Modified residuei177 – 1771Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96GQ7.
PaxDbiQ96GQ7.
PeptideAtlasiQ96GQ7.
PRIDEiQ96GQ7.

2D gel databases

SWISS-2DPAGEQ96GQ7.

PTM databases

PhosphoSiteiQ96GQ7.

Expressioni

Gene expression databases

ArrayExpressiQ96GQ7.
BgeeiQ96GQ7.
CleanExiHS_DDX27.
GenevestigatoriQ96GQ7.

Organism-specific databases

HPAiHPA047087.

Interactioni

Protein-protein interaction databases

BioGridi120793. 14 interactions.
IntActiQ96GQ7. 6 interactions.
MINTiMINT-1420816.

Structurei

3D structure databases

ProteinModelPortaliQ96GQ7.
SMRiQ96GQ7. Positions 218-581.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini249 – 423175Helicase ATP-binding
Add
BLAST
Domaini457 – 603147Helicase C-terminal
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi195 – 2006Nuclear localization signal Reviewed prediction
Motifi218 – 24629Q motif
Add
BLAST
Motifi371 – 3744DEAD box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi44 – 200157Asp/Glu/Lys-rich
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0513.
HOVERGENiHBG106162.
InParanoidiQ96GQ7.
KOiK13181.
OMAiQFCNITT.
OrthoDBiEOG7G1V5Q.
PhylomeDBiQ96GQ7.
TreeFamiTF314780.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96GQ7-1 [UniParc]FASTAAdd to Basket

« Hide

MVLAQRRRGG CEKLRAGPQA VLASGSGFCD NMLADLGLIG TIGEDDEVPV    50
EPESDSGDEE EEGPIVLGRR QKALGKNRSA DFNPDFVFTE KEGTYDGSWA 100
LADVMSQLKK KRAATTLDEK IEKVRKKRKT EDKEAKSGKL EKEKEAKEGS 150
EPKEQEDLQE NDEEGSEDEA SETDYSSADE NILTKADTLK VKDRKKKKKK 200
GQEAGGFFED ASQYDENLSF QDMNLSRPLL KAITAMGFKQ PTPIQKACIP 250
VGLLGKDICA CAATGTGKTA AFALPVLERL IYKPRQAPVT RVLVLVPTRE 300
LGIQVHSVTR QLAQFCNITT CLAVGGLDVK SQEAALRAAP DILIATPGRL 350
IDHLHNCPSF HLSSIEVLIL DEADRMLDEY FEEQMKEIIR MCSHHRQTML 400
FSATMTDEVK DLASVSLKNP VRIFVNSNTD VAPFLRQEFI RIRPNREGDR 450
EAIVAALLTR TFTDHVMLFT QTKKQAHRMH ILLGLMGLQV GELHGNLSQT 500
QRLEALRRFK DEQIDILVAT DVAARGLDIE GVKTVINFTM PNTIKHYVHR 550
VGRTARAGRA GRSVSLVGED ERKMLKEIVK AAKAPVKARI LPQDVILKFR 600
DKIEKMEKDV YAVLQLEAEE KEMQQSEAQI NTAKRLLEKG KEAVVQEPER 650
SWFQTKEERK KEKIAKALQE FDLALRGKKK RKKFMKDAKK KGEMTAEERS 700
QFEILKAQMF AERLAKRNRR AKRARAMPEE EPVRGPAKKQ KQGKKSVFDE 750
ELTNTSKKAL KQYRAGPSFE ERKQLGLPHQ RRGGNFKSKS RYKRRK 796
Length:796
Mass (Da):89,835
Last modified:March 28, 2003 - v2
Checksum:i9282C712B8F8B84A
GO

Sequence cautioni

The sequence AAF28937.1 differs from that shown. Reason: Sequencing errors.
The sequence AAF28937.1 differs from that shown. Reason: Frameshift at several positions.
The sequence AAG22482.1 differs from that shown. Reason: Frameshift at positions 208, 427, 434 and 532.
The sequence AAH16060.2 differs from that shown. Reason: Erroneous initiation.
The sequence BAA91284.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB14343.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti766 – 7661G → S.2 Publications
Corresponds to variant rs1130146 [ dbSNP | Ensembl ].
VAR_022849

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti482 – 4821L → F in AAK21271. 1 Publication
Sequence conflicti542 – 5421N → S in BAA91284. 1 Publication
Sequence conflicti548 – 5481V → D in AAK95821. 1 Publication
Sequence conflicti548 – 5481V → D in BAB14343. 1 Publication
Sequence conflicti674 – 6741A → T in AAK21271. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY044431 mRNA. Translation: AAK95821.1.
AF336851 mRNA. Translation: AAK21271.1.
AL049766, AL357560 Genomic DNA. Translation: CAI22427.1.
AL357560, AL049766 Genomic DNA. Translation: CAH70236.1.
BC009304 mRNA. Translation: AAH09304.2.
BC011927 mRNA. Translation: AAH11927.2.
BC016060 mRNA. Translation: AAH16060.2. Different initiation.
BC126287 mRNA. Translation: AAI26288.1.
BC130275 mRNA. Translation: AAI30276.1.
BC144125 mRNA. Translation: AAI44126.1.
AK022979 mRNA. Translation: BAB14343.1. Different initiation.
AK000603 mRNA. Translation: BAA91284.1. Different initiation.
AF193054 mRNA. Translation: AAG22482.1. Frameshift.
AF161377 mRNA. Translation: AAF28937.1. Sequence problems.
CCDSiCCDS13416.1.
RefSeqiNP_060365.7. NM_017895.7.
UniGeneiHs.129261.
Hs.65234.

Genome annotation databases

EnsembliENST00000371764; ENSP00000360828; ENSG00000124228.
GeneIDi55661.
KEGGihsa:55661.
UCSCiuc002xuh.3. human.

Polymorphism databases

DMDMi29427946.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY044431 mRNA. Translation: AAK95821.1 .
AF336851 mRNA. Translation: AAK21271.1 .
AL049766 , AL357560 Genomic DNA. Translation: CAI22427.1 .
AL357560 , AL049766 Genomic DNA. Translation: CAH70236.1 .
BC009304 mRNA. Translation: AAH09304.2 .
BC011927 mRNA. Translation: AAH11927.2 .
BC016060 mRNA. Translation: AAH16060.2 . Different initiation.
BC126287 mRNA. Translation: AAI26288.1 .
BC130275 mRNA. Translation: AAI30276.1 .
BC144125 mRNA. Translation: AAI44126.1 .
AK022979 mRNA. Translation: BAB14343.1 . Different initiation.
AK000603 mRNA. Translation: BAA91284.1 . Different initiation.
AF193054 mRNA. Translation: AAG22482.1 . Frameshift.
AF161377 mRNA. Translation: AAF28937.1 . Sequence problems.
CCDSi CCDS13416.1.
RefSeqi NP_060365.7. NM_017895.7.
UniGenei Hs.129261.
Hs.65234.

3D structure databases

ProteinModelPortali Q96GQ7.
SMRi Q96GQ7. Positions 218-581.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120793. 14 interactions.
IntActi Q96GQ7. 6 interactions.
MINTi MINT-1420816.

PTM databases

PhosphoSitei Q96GQ7.

Polymorphism databases

DMDMi 29427946.

2D gel databases

SWISS-2DPAGE Q96GQ7.

Proteomic databases

MaxQBi Q96GQ7.
PaxDbi Q96GQ7.
PeptideAtlasi Q96GQ7.
PRIDEi Q96GQ7.

Protocols and materials databases

DNASUi 55661.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000371764 ; ENSP00000360828 ; ENSG00000124228 .
GeneIDi 55661.
KEGGi hsa:55661.
UCSCi uc002xuh.3. human.

Organism-specific databases

CTDi 55661.
GeneCardsi GC20P047835.
HGNCi HGNC:15837. DDX27.
HPAi HPA047087.
neXtProti NX_Q96GQ7.
PharmGKBi PA27213.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0513.
HOVERGENi HBG106162.
InParanoidi Q96GQ7.
KOi K13181.
OMAi QFCNITT.
OrthoDBi EOG7G1V5Q.
PhylomeDBi Q96GQ7.
TreeFami TF314780.

Miscellaneous databases

ChiTaRSi DDX27. human.
GeneWikii DDX27.
GenomeRNAii 55661.
NextBioi 60399.
PROi Q96GQ7.

Gene expression databases

ArrayExpressi Q96GQ7.
Bgeei Q96GQ7.
CleanExi HS_DDX27.
Genevestigatori Q96GQ7.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Wang Y., Fan Y.-Z., Han W.-L., Yang T., Gao Y., Ma D.-L.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-766.
    Tissue: Brain, Muscle and Skin.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 24-796, VARIANT SER-766.
    Tissue: Stomach cancer and Teratocarcinoma.
  5. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
    Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
    , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 129-688.
  6. "Human partial CDS from CD34+ stem cells."
    Ye M., Zhang Q.-H., Zhou J., Shen Y., Wu X.-Y., Guan Z.Q., Wang L., Fan H.-Y., Mao Y.-F., Dai M., Huang Q.-H., Chen S.-J., Chen Z.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 600-796.
    Tissue: Umbilical cord blood.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79; SER-166 AND SER-177, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDDX27_HUMAN
AccessioniPrimary (citable) accession number: Q96GQ7
Secondary accession number(s): A0AVB6
, B7ZLY1, Q5VXM7, Q8WYG4, Q969N7, Q96F57, Q96L97, Q9BWY9, Q9BXF0, Q9H990, Q9NWU3, Q9P0C2, Q9UGD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: September 3, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-32 is the initiator.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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