ID   Q96GL6_HUMAN            Unreviewed;       385 AA.
AC   Q96GL6;
DT   01-DEC-2001, integrated into UniProtKB/TrEMBL.
DT   01-DEC-2001, sequence version 1.
DT   27-MAR-2024, entry version 159.
DE   SubName: Full=SLC18A1 protein {ECO:0000313|EMBL:AAH09387.1};
DE   SubName: Full=Solute carrier family 18 member A1 {ECO:0000313|Ensembl:ENSP00000428001.1};
GN   Name=SLC18A1 {ECO:0000313|EMBL:AAH09387.1,
GN   ECO:0000313|Ensembl:ENSP00000428001.1};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|EMBL:AAH09387.1};
RN   [1] {ECO:0000313|EMBL:AAH09387.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain {ECO:0000313|EMBL:AAH09387.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RA   Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H.,
RA   Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M.,
RA   Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M.,
RA   Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F.,
RA   Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T.,
RA   Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F.,
RA   Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E.,
RA   Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y.,
RA   Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E.,
RA   Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y.,
RA   Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W.,
RA   Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J.,
RA   Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W.,
RA   Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J.,
RA   Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I.,
RA   Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U.,
RA   Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A.,
RA   Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2] {ECO:0000313|Ensembl:ENSP00000428001.1, ECO:0000313|Proteomes:UP000005640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16421571; DOI=10.1038/nature04406;
RA   Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA   Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA   Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA   Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA   Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA   Engels R., Glockner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA   Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA   Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA   Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA   O'Neill K., Parker S.C., Polley A., Raymond C.K., Reichwald K.,
RA   Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA   Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA   Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA   Platzer M., Shimizu N., Lander E.S.;
RT   "DNA sequence and analysis of human chromosome 8.";
RL   Nature 439:331-335(2006).
RN   [3] {ECO:0000313|Ensembl:ENSP00000428001.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+)(out) + serotonin(in) = 2 H(+)(in) + serotonin(out);
CC         Xref=Rhea:RHEA:73743, ChEBI:CHEBI:15378, ChEBI:CHEBI:350546;
CC         Evidence={ECO:0000256|ARBA:ARBA00034441};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:73744;
CC         Evidence={ECO:0000256|ARBA:ARBA00034441};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dopamine(in) + 2 H(+)(out) = dopamine(out) + 2 H(+)(in);
CC         Xref=Rhea:RHEA:73739, ChEBI:CHEBI:15378, ChEBI:CHEBI:59905;
CC         Evidence={ECO:0000256|ARBA:ARBA00034439};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:73740;
CC         Evidence={ECO:0000256|ARBA:ARBA00034439};
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle membrane
CC       {ECO:0000256|ARBA:ARBA00004638}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004638}. Cytoplasmic vesicle, secretory
CC       vesicle, synaptic vesicle membrane {ECO:0000256|ARBA:ARBA00004644};
CC       Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004644}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Vesicular
CC       transporter family. {ECO:0000256|ARBA:ARBA00006829}.
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DR   EMBL; AC025853; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC009387; AAH09387.1; -; mRNA.
DR   RefSeq; NP_001129163.1; NM_001135691.2.
DR   RefSeq; NP_001135796.1; NM_001142324.1.
DR   RefSeq; NP_001135797.1; NM_001142325.1.
DR   RefSeq; NP_003044.1; NM_003053.3.
DR   ProteomicsDB; 76641; -.
DR   DNASU; 6570; -.
DR   Ensembl; ENST00000517776.5; ENSP00000428001.1; ENSG00000036565.15.
DR   GeneID; 6570; -.
DR   KEGG; hsa:6570; -.
DR   UCSC; uc064ktt.1; human.
DR   CTD; 6570; -.
DR   HGNC; HGNC:10934; SLC18A1.
DR   PharmGKB; PA324; -.
DR   VEuPathDB; HostDB:ENSG00000036565; -.
DR   GeneTree; ENSGT00940000159352; -.
DR   OrthoDB; 5388206at2759; -.
DR   BioGRID-ORCS; 6570; 13 hits in 1157 CRISPR screens.
DR   ChiTaRS; SLC18A1; human.
DR   GenomeRNAi; 6570; -.
DR   Proteomes; UP000005640; Chromosome 8.
DR   Bgee; ENSG00000036565; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 56 other cell types or tissues.
DR   GO; GO:0030672; C:synaptic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0051649; P:establishment of localization in cell; IEA:UniProt.
DR   GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR   GO; GO:0006837; P:serotonin transport; IEA:UniProt.
DR   Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR004734; Multidrug-R.
DR   NCBIfam; TIGR00880; 2_A_01_02; 1.
DR   PANTHER; PTHR23506; -; 1.
DR   PANTHER; PTHR23506:SF31; CHROMAFFIN GRANULE AMINE TRANSPORTER; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Neurotransmitter transport {ECO:0000256|ARBA:ARBA00022775};
KW   Proteomics identification {ECO:0007829|PeptideAtlas:Q96GL6,
KW   ECO:0007829|ProteomicsDB:Q96GL6};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005640};
KW   Synapse {ECO:0000256|ARBA:ARBA00023018};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022775}.
FT   TRANSMEM        21..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        164..186
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        198..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        232..253
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        260..281
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        301..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        338..357
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        363..383
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          23..385
FT                   /note="Major facilitator superfamily (MFS) profile"
FT                   /evidence="ECO:0000259|PROSITE:PS50850"
SQ   SEQUENCE   385 AA;  41172 MW;  DF4240D7F4CBC2BA CRC64;
     MLRTILDAPQ RLLKEGRASR QLVLVVVFVA LLLDNMLFTV VVPIVPTFLY DMEFKEVNSS
     LHLGHAGSSP HALASPAFST IFSFFNNNTV AVEESVPSGI AWMNDTASTI PPPATEAISA
     HKNNCLQGTG FLEEEITRVG VLFASKAVMQ LLVNPFVGPL TNRIGYHIPM FAGFVIMFLS
     TVMFAFSGTY TLLFVARTLQ GIGSSFSSVA GLGMLASVYT DDHERGRAMG TALGGLALGL
     LVGAPFGSVM YEFVGKSAPF LILAFLALLD GALQLCILQP SKVSPESAKG TPLFMLLKDP
     YILVAAGSIC FANMGVAILE PTLPIWMMQT MCSPKWQLGL AFLPASVSYL IGTNLFGVLA
     NKMGRWLCSL IGMLVVGTSL LCLPS
//