Q96GJ1 (TRM2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (uracil(54)-C(5))-methyltransferase homolog
EC=2.1.1.35

Alternative name(s):
TRM2 homolog

Gene names

Name:	TRMT2B
Synonyms:	CXorf34

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation By similarity.
Catalytic activity	S-adenosyl-L-methionine + uridine54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA.
Sequence similarities	Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	mitochondrion Inferred from electronic annotation. Source: Compara
Molecular_function	S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96GJ1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96GJ1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     464-504: LCCPPDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR → RSLILLECSGMVSAHCSLHLPGSSDSPASAS

Note: No experimental confirmation available.

Isoform 3 (identifier: Q96GJ1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     102-146: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	tRNA (uracil(54)-C(5))-methyltransferase homolog		PRO_0000311932
Sites
	Active site	451	1	Nucleophile By similarity
	Binding site	323	1	S-adenosyl-L-methionine By similarity
	Binding site	373	1	S-adenosyl-L-methionine By similarity
Natural variations
	Alternative sequence	102 – 146	45	Missing in isoform 3.		VSP_029643
	Alternative sequence	464 – 504	41	LCCPP…LLFTR → RSLILLECSGMVSAHCSLHL PGSSDSPASAS in isoform 2.		VSP_029644
	Natural variant	12	1	S → R. Corresponds to variant rs7064613 [ dbSNP | Ensembl ].		VAR_037355
Experimental info
	Sequence conflict	238	1	H → R in BAB14223. Ref.1
	Sequence conflict	451	1	C → R in CAI46112. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D7A28E168AC9C366	FASTA	504	56,476
	10 20 30 40 50 60 MAGLKRRVPL HSLRYFISMV GLFSKPGLLP WYARNPPGWS QLFLGTVCKG DFTRVIATKC 70 80 90 100 110 120 QKGQKSQKKP SHLGPLDGSW QERLADVVTP LWRLSYEEQL KVKFAAQKKI LQRLESYIQM 130 140 150 160 170 180 LNGVSVTTAV PKSERLSCLL HPIIPSPVIN GYRNKSTFSV NRGPDGNPKT VGFYLGTWRD 190 200 210 220 230 240 GNVVCVQSNH LKNIPEKHSQ VAQYYEVFLR QSPLEPCLVF HEGGYWRELT VRTNSQGHTM 250 260 270 280 290 300 AIITFHPQKL SQEELHVQKE IVKEFFIRGP GAACGLTSLY FQESTMTRCS HQQSPYQLLF 310 320 330 340 350 360 GEPYIFEELL SLKIRISPDA FFQINTAGAE MLYRTVGELT GVNSDTILLD ICCGTGVIGL 370 380 390 400 410 420 SLAQHTSRVL GIELLEQAVE DARWTAAFNG ITNSEFHTGQ AEKILPGLLK SKEDGQSIVA 430 440 450 460 470 480 VVNPARAGLH YKVIQAIRNF RAIHTLVFVS CKLHGESTRN VIELCCPPDP AKKLLGEPFV 490 500 LQQAVPVDLF PHTPHCELVL LFTR
	Isoform 2 [UniParc]. Checksum: 22B3CD7F012C136F	FASTA	494	55,006
	10 20 30 40 50 60 MAGLKRRVPL HSLRYFISMV GLFSKPGLLP WYARNPPGWS QLFLGTVCKG DFTRVIATKC 70 80 90 100 110 120 QKGQKSQKKP SHLGPLDGSW QERLADVVTP LWRLSYEEQL KVKFAAQKKI LQRLESYIQM 130 140 150 160 170 180 LNGVSVTTAV PKSERLSCLL HPIIPSPVIN GYRNKSTFSV NRGPDGNPKT VGFYLGTWRD 190 200 210 220 230 240 GNVVCVQSNH LKNIPEKHSQ VAQYYEVFLR QSPLEPCLVF HEGGYWRELT VRTNSQGHTM 250 260 270 280 290 300 AIITFHPQKL SQEELHVQKE IVKEFFIRGP GAACGLTSLY FQESTMTRCS HQQSPYQLLF 310 320 330 340 350 360 GEPYIFEELL SLKIRISPDA FFQINTAGAE MLYRTVGELT GVNSDTILLD ICCGTGVIGL 370 380 390 400 410 420 SLAQHTSRVL GIELLEQAVE DARWTAAFNG ITNSEFHTGQ AEKILPGLLK SKEDGQSIVA 430 440 450 460 470 480 VVNPARAGLH YKVIQAIRNF RAIHTLVFVS CKLHGESTRN VIERSLILLE CSGMVSAHCS 490 LHLPGSSDSP ASAS
	Isoform 3 [UniParc]. Checksum: 2000E09C0715483C	FASTA	459	51,485
	10 20 30 40 50 60 MAGLKRRVPL HSLRYFISMV GLFSKPGLLP WYARNPPGWS QLFLGTVCKG DFTRVIATKC 70 80 90 100 110 120 QKGQKSQKKP SHLGPLDGSW QERLADVVTP LWRLSYEEQL KPVINGYRNK STFSVNRGPD 130 140 150 160 170 180 GNPKTVGFYL GTWRDGNVVC VQSNHLKNIP EKHSQVAQYY EVFLRQSPLE PCLVFHEGGY 190 200 210 220 230 240 WRELTVRTNS QGHTMAIITF HPQKLSQEEL HVQKEIVKEF FIRGPGAACG LTSLYFQEST 250 260 270 280 290 300 MTRCSHQQSP YQLLFGEPYI FEELLSLKIR ISPDAFFQIN TAGAEMLYRT VGELTGVNSD 310 320 330 340 350 360 TILLDICCGT GVIGLSLAQH TSRVLGIELL EQAVEDARWT AAFNGITNSE FHTGQAEKIL 370 380 390 400 410 420 PGLLKSKEDG QSIVAVVNPA RAGLHYKVIQ AIRNFRAIHT LVFVSCKLHG ESTRNVIELC 430 440 450 CPPDPAKKLL GEPFVLQQAV PVDLFPHTPH CELVLLFTR

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Teratocarcinoma.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Lymph node.
[3]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain, Colon, Lymph, Placenta and Skin.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK022749 mRNA. Translation: BAB14223.1. AL832849 mRNA. Translation: CAI46112.1. AL133275, AL109952, Z97985 Genomic DNA. Translation: CAI42155.1. AL133275, AL109952, Z97985 Genomic DNA. Translation: CAI42156.1. Z97985, AL109952, AL133275 Genomic DNA. Translation: CAI42658.1. Z97985, AL109952, AL133275 Genomic DNA. Translation: CAI42657.1. AL109952, AL133275, Z97985 Genomic DNA. Translation: CAI42997.1. AL109952, AL133275, Z97985 Genomic DNA. Translation: CAI42998.1. CH471115 Genomic DNA. Translation: EAX02831.1. CH471115 Genomic DNA. Translation: EAX02837.1. BC007526 mRNA. Translation: AAH07526.1. BC008067 mRNA. Translation: AAH08067.2. BC009437 mRNA. Translation: AAH09437.1. BC020116 mRNA. Translation: AAH20116.1. BC034272 mRNA. Translation: AAH34272.1.
IPI	IPI00550091. IPI00747797. IPI00749054.
RefSeq	NP_001161442.1. NM_001167970.1. NP_001161443.1. NM_001167971.1. NP_001161444.1. NM_001167972.1. NP_079193.2. NM_024917.5.
UniGene	Hs.496501.
3D structure databases
ProteinModelPortal	Q96GJ1.
ModBase	Search...
PTM databases
PhosphoSite	Q96GJ1.
Polymorphism databases
DMDM	74762656.
Proteomic databases
PaxDb	Q96GJ1.
PRIDE	Q96GJ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338687; ENSP00000340970; ENSG00000188917. ENST00000372935; ENSP00000362026; ENSG00000188917. ENST00000372936; ENSP00000362027; ENSG00000188917. ENST00000372939; ENSP00000362030; ENSG00000188917. ENST00000545398; ENSP00000438134; ENSG00000188917.
GeneID	79979.
KEGG	hsa:79979.
UCSC	uc004egq.3. human. uc004egv.3. human.
Organism-specific databases
CTD	79979.
GeneCards	GC0XM100264.
H-InvDB	HIX0016920. HIX0029044.
HGNC	HGNC:25748. TRMT2B.
HPA	HPA035120.
neXtProt	NX_Q96GJ1.
PharmGKB	PA164726782.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2265.
HOVERGEN	HBG108599.
InParanoid	Q96GJ1.
KO	K15331.
OMA	STMTRCS.
PhylomeDB	Q96GJ1.
Gene expression databases
ArrayExpress	Q96GJ1.
Bgee	Q96GJ1.
CleanEx	HS_TRMT2B.
Genevestigator	Q96GJ1.
Family and domain databases
InterPro	IPR025714. Methyltranfer_dom. IPR025823. tRNA_(uracil-5-)_MeTrfase_met. [Graphical view]
Pfam	PF13847. Methyltransf_31. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TRMT2B. human.
GenomeRNAi	79979.
NextBio	70009.

Entry information

Entry name

TRM2_HUMAN

Accession

Primary (citable) accession number: Q96GJ1
Secondary accession number(s): A6NDG5 , A6NEI9, A6NMG6, Q5JPF0, Q5JVY6, Q96HU7, Q96IH9, Q9H9K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.