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Protein

DCN1-like protein 1

Gene

DCUN1D1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates (PubMed:25349211). Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
DCN1-like protein 1
Alternative name(s):
DCUN1 domain-containing protein 1
Defective in cullin neddylation protein 1-like protein 1
Squamous cell carcinoma-related oncogene
Gene namesi
Name:DCUN1D1
Synonyms:DCUN1L1, RP42, SCCRO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:18184. DCUN1D1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi241D → N: Loss of binding to CAND1 and CUL-RBX1 complex but retains binding to UBE2M. 1 Publication1

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi54165.
OpenTargetsiENSG00000043093.
PharmGKBiPA142672008.

Polymorphism and mutation databases

BioMutaiDCUN1D1.
DMDMi73919222.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001294981 – 259DCN1-like protein 1Add BLAST259

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ96GG9.
MaxQBiQ96GG9.
PaxDbiQ96GG9.
PeptideAtlasiQ96GG9.
PRIDEiQ96GG9.

PTM databases

iPTMnetiQ96GG9.
PhosphoSitePlusiQ96GG9.

Expressioni

Tissue specificityi

Expressed in pancreas, kidney, placenta, brain and heart. Weakly or not expressed in liver, skeletal muscle and lung. Strongly overexpressed in thyroid tumors, bronchioloalveolar carcinomas, and malignant tissues of squamous cell carcinoma of the oral tongue. Not overexpressed in aggressive adrenocortical carcinomas.1 Publication

Gene expression databases

BgeeiENSG00000043093.
CleanExiHS_DCUN1D1.
ExpressionAtlasiQ96GG9. baseline and differential.
GenevisibleiQ96GG9. HS.

Organism-specific databases

HPAiHPA035911.

Interactioni

Subunit structurei

Part of an E3 complex for neddylation composed of cullins, RBX1, UBE2M and CAND1. Interacts (via the C-terminus 50 AA) with CUL1, CUL2, CUL3, CUL4 and CUL5. Binds neddylated CUL1. Interacts (via the C-terminus 50 AA) directly with RBX1. Interacts preferentially with UBE2M-NEDD8 thioester (via N-terminus 1-26 AA) than with free UBE2M. UBE2M N-terminal acetylation increases the affinity of this interaction by about 2 orders of magnitude. Interacts with CAND1 when in complex with CUL1-RBX1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARRDC3Q96B675EBI-740086,EBI-2875665
DAZAP2Q150386EBI-740086,EBI-724310
LZTS2Q9BRK45EBI-740086,EBI-741037
TP53BP2Q13625-33EBI-740086,EBI-10175039
TRIM39Q9HCM94EBI-740086,EBI-739510
TRIM39Q9HCM9-24EBI-740086,EBI-11523450
TRIM54Q9BYV23EBI-740086,EBI-2130429
VPS37BQ9H9H43EBI-740086,EBI-4400866

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119920. 237 interactors.
DIPiDIP-42121N.
IntActiQ96GG9. 21 interactors.
MINTiMINT-1182942.
STRINGi9606.ENSP00000292782.

Structurei

Secondary structure

1259
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi62 – 72Combined sources11
Beta strandi77 – 81Combined sources5
Helixi83 – 93Combined sources11
Helixi100 – 108Combined sources9
Beta strandi116 – 118Combined sources3
Helixi119 – 129Combined sources11
Helixi134 – 139Combined sources6
Helixi141 – 147Combined sources7
Helixi151 – 165Combined sources15
Beta strandi171 – 174Combined sources4
Helixi175 – 185Combined sources11
Turni187 – 189Combined sources3
Helixi193 – 202Combined sources10
Helixi210 – 222Combined sources13
Beta strandi232 – 236Combined sources5
Helixi238 – 250Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TDUX-ray1.50A/B62-259[»]
3TDZX-ray2.00A/B62-259[»]
4P5OX-ray3.11E/F62-259[»]
ProteinModelPortaliQ96GG9.
SMRiQ96GG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 45UBA-likeAdd BLAST38
Domaini60 – 248DCUN1PROSITE-ProRule annotationAdd BLAST189

Sequence similaritiesi

Contains 1 DCUN1 domain.PROSITE-ProRule annotation
Contains 1 UBA-like domain.Curated

Phylogenomic databases

eggNOGiKOG3077. Eukaryota.
ENOG410XTIJ. LUCA.
GeneTreeiENSGT00550000074529.
HOGENOMiHOG000241761.
HOVERGENiHBG055256.
InParanoidiQ96GG9.
KOiK17822.
OMAiEQTAIYC.
OrthoDBiEOG091G0HHH.
PhylomeDBiQ96GG9.
TreeFamiTF313332.

Family and domain databases

InterProiIPR014764. DCN-prot.
IPR005176. PONY_dom.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR12281. PTHR12281. 1 hit.
PfamiPF03556. Cullin_binding. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS51229. DCUN1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96GG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKLKSSQKD KVRQFMIFTQ SSEKTAVSCL SQNDWKLDVA TDNFFQNPEL
60 70 80 90 100
YIRESVKGSL DRKKLEQLYN RYKDPQDENK IGIDGIQQFC DDLALDPASI
110 120 130 140 150
SVLIIAWKFR AATQCEFSKQ EFMDGMTELG CDSIEKLKAQ IPKMEQELKE
160 170 180 190 200
PGRFKDFYQF TFNFAKNPGQ KGLDLEMAIA YWNLVLNGRF KFLDLWNKFL
210 220 230 240 250
LEHHKRSIPK DTWNLLLDFS TMIADDMSNY DEEGAWPVLI DDFVEFARPQ

IAGTKSTTV
Length:259
Mass (Da):30,124
Last modified:December 1, 2001 - v1
Checksum:iF3709235E0610C54
GO

Sequence cautioni

The sequence BAB15235 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134I → T in AAL78673 (PubMed:17018598).Curated1
Sequence conflicti136K → Q in AAG00606 (PubMed:10777668).Curated1
Sequence conflicti191 – 192KF → RL in AAL78673 (PubMed:17018598).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF292100 mRNA. Translation: AAG00606.2.
AF456425 mRNA. Translation: AAL78672.1.
AF456426 mRNA. Translation: AAL78673.1.
AK025764 mRNA. Translation: BAB15235.1. Different initiation.
AK056335 mRNA. Translation: BAG51680.1.
AK314480 mRNA. Translation: BAG37084.1.
CH471052 Genomic DNA. Translation: EAW78342.1.
BC009478 mRNA. Translation: AAH09478.1.
BC013163 mRNA. Translation: AAH13163.2.
CCDSiCCDS3240.1.
RefSeqiNP_001295030.1. NM_001308101.1.
NP_065691.2. NM_020640.3.
UniGeneiHs.592789.
Hs.744153.

Genome annotation databases

EnsembliENST00000292782; ENSP00000292782; ENSG00000043093.
GeneIDi54165.
KEGGihsa:54165.
UCSCiuc003fld.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF292100 mRNA. Translation: AAG00606.2.
AF456425 mRNA. Translation: AAL78672.1.
AF456426 mRNA. Translation: AAL78673.1.
AK025764 mRNA. Translation: BAB15235.1. Different initiation.
AK056335 mRNA. Translation: BAG51680.1.
AK314480 mRNA. Translation: BAG37084.1.
CH471052 Genomic DNA. Translation: EAW78342.1.
BC009478 mRNA. Translation: AAH09478.1.
BC013163 mRNA. Translation: AAH13163.2.
CCDSiCCDS3240.1.
RefSeqiNP_001295030.1. NM_001308101.1.
NP_065691.2. NM_020640.3.
UniGeneiHs.592789.
Hs.744153.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TDUX-ray1.50A/B62-259[»]
3TDZX-ray2.00A/B62-259[»]
4P5OX-ray3.11E/F62-259[»]
ProteinModelPortaliQ96GG9.
SMRiQ96GG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119920. 237 interactors.
DIPiDIP-42121N.
IntActiQ96GG9. 21 interactors.
MINTiMINT-1182942.
STRINGi9606.ENSP00000292782.

PTM databases

iPTMnetiQ96GG9.
PhosphoSitePlusiQ96GG9.

Polymorphism and mutation databases

BioMutaiDCUN1D1.
DMDMi73919222.

Proteomic databases

EPDiQ96GG9.
MaxQBiQ96GG9.
PaxDbiQ96GG9.
PeptideAtlasiQ96GG9.
PRIDEiQ96GG9.

Protocols and materials databases

DNASUi54165.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292782; ENSP00000292782; ENSG00000043093.
GeneIDi54165.
KEGGihsa:54165.
UCSCiuc003fld.2. human.

Organism-specific databases

CTDi54165.
DisGeNETi54165.
GeneCardsiDCUN1D1.
HGNCiHGNC:18184. DCUN1D1.
HPAiHPA035911.
MIMi605905. gene.
neXtProtiNX_Q96GG9.
OpenTargetsiENSG00000043093.
PharmGKBiPA142672008.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3077. Eukaryota.
ENOG410XTIJ. LUCA.
GeneTreeiENSGT00550000074529.
HOGENOMiHOG000241761.
HOVERGENiHBG055256.
InParanoidiQ96GG9.
KOiK17822.
OMAiEQTAIYC.
OrthoDBiEOG091G0HHH.
PhylomeDBiQ96GG9.
TreeFamiTF313332.

Miscellaneous databases

ChiTaRSiDCUN1D1. human.
GeneWikiiDCUN1D1.
GenomeRNAii54165.
PROiQ96GG9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000043093.
CleanExiHS_DCUN1D1.
ExpressionAtlasiQ96GG9. baseline and differential.
GenevisibleiQ96GG9. HS.

Family and domain databases

InterProiIPR014764. DCN-prot.
IPR005176. PONY_dom.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR12281. PTHR12281. 1 hit.
PfamiPF03556. Cullin_binding. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS51229. DCUN1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCNL1_HUMAN
AccessioniPrimary (citable) accession number: Q96GG9
Secondary accession number(s): B2RB37
, Q7L3G9, Q8TEX7, Q9H6M1, Q9HCT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.