Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase RNF185

Gene

RNF185

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521).3 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri39 – 80RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin-like protein conjugating enzyme binding Source: ParkinsonsUK-UCL
  • ubiquitin protein ligase activity Source: ParkinsonsUK-UCL

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • endoplasmic reticulum mannose trimming Source: Reactome
  • ERAD pathway Source: ParkinsonsUK-UCL
  • ER-associated misfolded protein catabolic process Source: GO_Central
  • protein autoubiquitination Source: ParkinsonsUK-UCL
  • transmembrane transport Source: Reactome
  • ubiquitin-dependent ERAD pathway Source: ParkinsonsUK-UCL

Keywordsi

Molecular functionTransferase
Biological processAutophagy, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-382556 ABC-family proteins mediated transport
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-901032 ER Quality Control Compartment (ERQC)
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF185 (EC:2.3.2.272 Publications)
Alternative name(s):
RING finger protein 185
RING-type E3 ubiquitin transferase RNF185Curated
Gene namesi
Name:RNF185
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000138942.15
HGNCiHGNC:26783 RNF185
neXtProtiNX_Q96GF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 130Cytoplasmic1 PublicationAdd BLAST130
Transmembranei131 – 151HelicalSequence analysisAdd BLAST21
Topological domaini152 – 171Mitochondrial intermembrane1 PublicationAdd BLAST20
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39C → S: Decreased ubiquitin ligase activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000138942
PharmGKBiPA142671056

Polymorphism and mutation databases

BioMutaiRNF185
DMDMi74751883

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002475201 – 192E3 ubiquitin-protein ligase RNF185Add BLAST192

Proteomic databases

EPDiQ96GF1
MaxQBiQ96GF1
PaxDbiQ96GF1
PeptideAtlasiQ96GF1
PRIDEiQ96GF1
ProteomicsDBi76632
76633 [Q96GF1-2]
TopDownProteomicsiQ96GF1-1 [Q96GF1-1]
Q96GF1-2 [Q96GF1-2]

PTM databases

iPTMnetiQ96GF1
PhosphoSitePlusiQ96GF1

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Inductioni

Up-regulated by unfolded protein response (UPR) and endoplasmic reticulum (ER) stress triggered by thapsigargin or tunicamycin.2 Publications

Gene expression databases

BgeeiENSG00000138942
CleanExiHS_RNF185
ExpressionAtlasiQ96GF1 baseline and differential
GenevisibleiQ96GF1 HS

Interactioni

Subunit structurei

Interacts with ATG5 and BNIP1.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin-like protein conjugating enzyme binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi124834, 70 interactors
IntActiQ96GF1, 31 interactors
STRINGi9606.ENSP00000320508

Structurei

3D structure databases

ProteinModelPortaliQ96GF1
SMRiQ96GF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 80Required for ubiquitin ligase activity and protection against ER stress-induced cell death1 PublicationAdd BLAST52

Domaini

The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri39 – 80RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG0823 Eukaryota
ENOG4111IHV LUCA
GeneTreeiENSGT00760000119075
HOGENOMiHOG000238304
HOVERGENiHBG054495
InParanoidiQ96GF1
KOiK10666
OMAiDGGFHMS
OrthoDBiEOG091G0W2I
PhylomeDBiQ96GF1
TreeFamiTF317334

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96GF1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKGPSASA SPENSSAGGP SGSSNGAGES GGQDSTFECN ICLDTAKDAV
60 70 80 90 100
ISLCGHLFCW PCLHQWLETR PNRQVCPVCK AGISRDKVIP LYGRGSTGQQ
110 120 130 140 150
DPREKTPPRP QGQRPEPENR GGFQGFGFGD GGFQMSFGIG AFPFGIFATA
160 170 180 190
FNINDGRPPP AVPGTPQYVD EQFLSRLFLF VALVIMFWLL IA
Length:192
Mass (Da):20,459
Last modified:December 1, 2001 - v1
Checksum:iF24B49EA566740B3
GO
Isoform 2 (identifier: Q96GF1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     66-121: Missing.

Note: No experimental confirmation available.
Show »
Length:136
Mass (Da):14,139
Checksum:i00AAB3A853E80773
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02000466 – 121Missing in isoform 2. 2 PublicationsAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ296559 mRNA Translation: ABB97506.1
DQ296561 mRNA Translation: ABB97508.1
DQ296562 mRNA Translation: ABB97509.1
DQ296565 mRNA Translation: ABB97512.1
CR456349 mRNA Translation: CAG30235.1
AK095947 mRNA Translation: BAC04659.1
AK291236 mRNA Translation: BAF83925.1
AC002073 Genomic DNA No translation available.
AC005005 Genomic DNA No translation available.
CH471095 Genomic DNA Translation: EAW59947.1
CH471095 Genomic DNA Translation: EAW59949.1
BC009504 mRNA Translation: AAH09504.1
BC026040 mRNA Translation: AAH26040.1
BC033166 mRNA Translation: AAH33166.1
BC012817 mRNA Translation: AAH12817.1
BC035684 mRNA Translation: AAH35684.1
CCDSiCCDS13890.1 [Q96GF1-1]
CCDS46689.1 [Q96GF1-2]
RefSeqiNP_001129297.1, NM_001135825.1 [Q96GF1-2]
NP_689480.2, NM_152267.3 [Q96GF1-1]
UniGeneiHs.517553

Genome annotation databases

EnsembliENST00000266252; ENSP00000266252; ENSG00000138942 [Q96GF1-2]
ENST00000326132; ENSP00000320508; ENSG00000138942 [Q96GF1-1]
ENST00000518626; ENSP00000427755; ENSG00000138942 [Q96GF1-1]
GeneIDi91445
KEGGihsa:91445
UCSCiuc003akb.4 human [Q96GF1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRN185_HUMAN
AccessioniPrimary (citable) accession number: Q96GF1
Secondary accession number(s): A8K5C1, A9X3T8, Q8N900
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: December 1, 2001
Last modified: June 20, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health