Q96GE6 (CALL4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calmodulin-like protein 4

Alternative name(s):
Serologically defined breast cancer antigen NY-BR-20

Gene names

Name:

CALML4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Tissue specificity	Expressed in breast cancer cell lines. Ref.1
Sequence similarities	Belongs to the calmodulin family. Contains 4 EF-hand domains.
Sequence caution	The sequence AAG48255.1 differs from that shown. Reason: Frameshift at several positions. The sequence BAG59377.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96GE6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96GE6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Isoform 3 (identifier: Q96GE6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-115: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Calmodulin-like protein 4		PRO_0000314933
Regions
	Domain	51 – 86	36	EF-hand 1
	Domain	87 – 122	36	EF-hand 2
	Domain	124 – 159	36	EF-hand 3
	Domain	160 – 195	36	EF-hand 4
Natural variations
	Alternative sequence	1 – 115	115	Missing in isoform 3.		VSP_030435
	Alternative sequence	1 – 76	76	Missing in isoform 2.		VSP_030436
	Natural variant	28	1	R → C. Corresponds to variant rs3803381 [ dbSNP | Ensembl ].		VAR_048586
	Natural variant	154	1	T → K. Corresponds to variant rs2280217 [ dbSNP | Ensembl ].		VAR_048587
Experimental info
	Sequence conflict	40	1	R → G in AAG48255. Ref.1
	Sequence conflict	60	1	F → S in AAG48255. Ref.1
	Sequence conflict	76	1	L → F in AAG48255. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 14, 2008. Version 3. Checksum: 547C558AC7EDFABD	FASTA	196	21,883
	10 20 30 40 50 60 MAAEHLLPGP PPSLADFRLE AGGKGTERGS GSSKPTGSSR GPRMAKFLSQ DQINEYKECF 70 80 90 100 110 120 SLYDKQQRGK IKATDLMVAM RCLGASPTPG EVQRHLQTHG IDGNGELDFS TFLTIMHMQI 130 140 150 160 170 180 KQEDPKKEIL LAMLMVDKEK KGYVMASDLR SKLTSLGEKL THKEVDDLFR EADIEPNGKV 190 KYDEFIHKIT LPGRDY
	Isoform 2 [UniParc]. Checksum: 8409F82E7385FB69	FASTA	120	13,661
	10 20 30 40 50 60 MVAMRCLGAS PTPGEVQRHL QTHGIDGNGE LDFSTFLTIM HMQIKQEDPK KEILLAMLMV 70 80 90 100 110 120 DKEKKGYVMA SDLRSKLTSL GEKLTHKEVD DLFREADIEP NGKVKYDEFI HKITLPGRDY
	Isoform 3 [UniParc]. Checksum: F176C12A51C39F12	FASTA	81	9,478
	10 20 30 40 50 60 MHMQIKQEDP KKEILLAMLM VDKEKKGYVM ASDLRSKLTS LGEKLTHKEV DDLFREADIE 70 80 PNGKVKYDEF IHKITLPGRD Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression." Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O., Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J. Cancer Immun. 1:4-4(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Mammary gland.
[2]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Prostate.
[4]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Colon.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-196 (ISOFORM 1).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF308287 mRNA. Translation: AAG48255.1. Sequence problems. CR598967 mRNA. No translation available. BX640702 mRNA. Translation: CAE45822.1. AC107871 Genomic DNA. No translation available. CH471082 Genomic DNA. Translation: EAW77810.1. BC009516 mRNA. No translation available. AK296804 mRNA. Translation: BAG59377.1. Different initiation.
IPI	IPI00478437. IPI00789951. IPI00791138.
RefSeq	NP_001026903.2. NM_001031733.2. NP_219501.2. NM_033429.2.
UniGene	Hs.709550.
3D structure databases
ProteinModelPortal	Q96GE6.
ModBase	Search...
PTM databases
PhosphoSite	Q96GE6.
Polymorphism databases
DMDM	209572745.
Proteomic databases
PaxDb	Q96GE6.
PRIDE	Q96GE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000467889; ENSP00000419081; ENSG00000129007. ENST00000540479; ENSP00000438177; ENSG00000129007.
GeneID	91860.
KEGG	hsa:91860.
UCSC	uc002arb.3. human.
Organism-specific databases
CTD	91860.
GeneCards	GC15M068483.
H-InvDB	HIX0012375.
HGNC	HGNC:18445. CALML4.
HPA	HPA051109.
neXtProt	NX_Q96GE6.
PharmGKB	PA134903345.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5126.
HOGENOM	HOG000233018.
HOVERGEN	HBG012180.
InParanoid	Q96GE6.
OMA	VMRCLGT.
Gene expression databases
ArrayExpress	Q96GE6.
Bgee	Q96GE6.
CleanEx	HS_CALML4.
Genevestigator	Q96GE6.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13499. EF_hand_5. 1 hit. [Graphical view]
SMART	SM00054. EFh. 4 hits. [Graphical view]
PROSITE	PS50222. EF_HAND_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	91860.
NextBio	77492.

Entry information

Entry name

CALL4_HUMAN

Accession

Primary (citable) accession number: Q96GE6
Secondary accession number(s): B4DL15, Q6N048, Q9H286

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 14, 2008
Last modified:	May 29, 2013
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.