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Protein

Neurotrophin receptor-interacting factor homolog

Gene

ZNF274

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription repressor. Specifically binds to the 3'-end of zinc-finger coding genes and recruiting chromatin-modifying proteins such as SETDB1 and TRIM28/KAP1, leading to transcription repression. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri507 – 529C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri535 – 557C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri563 – 585C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri591 – 613C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri619 – 641C2H2-type 5PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • neurotrophin p75 receptor binding Source: GO_Central
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: GO_Central
  • sequence-specific DNA binding Source: UniProtKB
  • transcription corepressor activity Source: ProtInc
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc

GO - Biological processi

  • regulation of histone H3-K9 trimethylation Source: UniProtKB
  • regulation of transcription, DNA-templated Source: ProtInc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000171606-MONOMER.
ReactomeiR-HSA-212436. Generic Transcription Pathway.
SignaLinkiQ96GC6.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurotrophin receptor-interacting factor homolog
Alternative name(s):
Zinc finger protein 274
Zinc finger protein HFB101
Zinc finger protein with KRAB and SCAN domains 19
Zinc finger protein zfp2
Short name:
Zf2
Gene namesi
Name:ZNF274
Synonyms:ZKSCAN19
ORF Names:SP2114
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:13068. ZNF274.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleusnucleolus 1 Publication

GO - Cellular componenti

  • centrosome Source: HPA
  • cytoplasm Source: UniProtKB-SubCell
  • nucleolus Source: ProtInc
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10782.
OpenTargetsiENSG00000171606.
PharmGKBiPA37644.

Polymorphism and mutation databases

BioMutaiZNF274.
DMDMi327478548.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000475011 – 653Neurotrophin receptor-interacting factor homologAdd BLAST653

Proteomic databases

MaxQBiQ96GC6.
PaxDbiQ96GC6.
PeptideAtlasiQ96GC6.
PRIDEiQ96GC6.

PTM databases

iPTMnetiQ96GC6.
PhosphoSitePlusiQ96GC6.

Expressioni

Gene expression databases

BgeeiENSG00000171606.
CleanExiHS_ZNF274.
ExpressionAtlasiQ96GC6. baseline and differential.
GenevisibleiQ96GC6. HS.

Organism-specific databases

HPAiHPA028880.

Interactioni

Subunit structurei

Interacts with SETDB1 and TRIM28/KAP1. Interacts with ATRX. Forms a complex with ATRX, SETDB1 and TRIM28 (PubMed:27029610).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115999. 11 interactors.
IntActiQ96GC6. 2 interactors.
STRINGi9606.ENSP00000321209.

Structurei

3D structure databases

ProteinModelPortaliQ96GC6.
SMRiQ96GC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 85KRAB 1PROSITE-ProRule annotationAdd BLAST72
Domaini161 – 243SCAN boxPROSITE-ProRule annotationAdd BLAST83
Domaini287 – 360KRAB 2PROSITE-ProRule annotationAdd BLAST74

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 2 KRAB domains.PROSITE-ProRule annotation
Contains 1 SCAN box domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri507 – 529C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri535 – 557C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri563 – 585C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri591 – 613C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri619 – 641C2H2-type 5PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00680000099963.
HOVERGENiHBG018163.
InParanoidiQ96GC6.
KOiK12458.
OMAiTAWSCEP.
OrthoDBiEOG091G02MB.
PhylomeDBiQ96GC6.
TreeFamiTF338018.

Family and domain databases

CDDicd07765. KRAB_A-box. 2 hits.
Gene3Di3.30.160.60. 5 hits.
InterProiIPR001909. KRAB.
IPR008916. Retrov_capsid_C.
IPR003309. SCAN_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 2 hits.
PF02023. SCAN. 1 hit.
PF00096. zf-C2H2. 3 hits.
[Graphical view]
SMARTiSM00349. KRAB. 2 hits.
SM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 5 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 2 hits.
SSF47353. SSF47353. 1 hit.
PROSITEiPS50805. KRAB. 2 hits.
PS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q96GC6-1) [UniParc]FASTAAdd to basket
Also known as: SP2114a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASRLPTAWS CEPVTFEDVT LGFTPEEWGL LDLKQKSLYR EVMLENYRNL
60 70 80 90 100
VSVEHQLSKP DVVSQLEEAE DFWPVERGIP QDTIPEYPEL QLDPKLDPLP
110 120 130 140 150
AESPLMNIEV VEVLTLNQEV AGPRNAQIQA LYAEDGSLSA DAPSEQVQQQ
160 170 180 190 200
GKHPGDPEAA RQRFRQFRYK DMTGPREALD QLRELCHQWL QPKARSKEQI
210 220 230 240 250
LELLVLEQFL GALPVKLRTW VESQHPENCQ EVVALVEGVT WMSEEEVLPA
260 270 280 290 300
GQPAEGTTCC LEVTAQQEEK QEDAAICPVT VLPEEPVTFQ DVAVDFSREE
310 320 330 340 350
WGLLGPTQRT EYRDVMLETF GHLVSVGWET TLENKELAPN SDIPEEEPAP
360 370 380 390 400
SLKVQESSRD CALSSTLEDT LQGGVQEVQD TVLKQMESAQ EKDLPQKKHF
410 420 430 440 450
DNRESQANSG ALDTNQVSLQ KIDNPESQAN SGALDTNQVL LHKIPPRKRL
460 470 480 490 500
RKRDSQVKSM KHNSRVKIHQ KSCERQKAKE GNGCRKTFSR STKQITFIRI
510 520 530 540 550
HKGSQVCRCS ECGKIFRNPR YFSVHKKIHT GERPYVCQDC GKGFVQSSSL
560 570 580 590 600
TQHQRVHSGE RPFECQECGR TFNDRSAISQ HLRTHTGAKP YKCQDCGKAF
610 620 630 640 650
RQSSHLIRHQ RTHTGERPYA CNKCGKAFTQ SSHLIGHQRT HNRTKRKKKQ

PTS
Length:653
Mass (Da):74,177
Last modified:April 5, 2011 - v2
Checksum:i8B91F51F4BEED925
GO
Isoform 2 (identifier: Q96GC6-2) [UniParc]FASTAAdd to basket
Also known as: ZNF274a

The sequence of this isoform differs from the canonical sequence as follows:
     55-86: Missing.

Show »
Length:621
Mass (Da):70,505
Checksum:i8B3B3F12B341A136
GO
Isoform 3 (identifier: Q96GC6-3) [UniParc]FASTAAdd to basket
Also known as: ZNF274b

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.

Show »
Length:548
Mass (Da):62,113
Checksum:i1A8C8BFE6FB17B69
GO
Isoform 4 (identifier: Q96GC6-4) [UniParc]FASTAAdd to basket
Also known as: SP2114b

The sequence of this isoform differs from the canonical sequence as follows:
     1-281: Missing.
     282-284: LPE → MLQ

Show »
Length:372
Mass (Da):42,448
Checksum:iF7D35AECFECCF7CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14V → E in AAG24390 (PubMed:15498874).Curated1
Sequence conflicti212A → T in AAG24390 (PubMed:15498874).Curated1
Sequence conflicti289F → S in CAE46074 (PubMed:17974005).Curated1
Sequence conflicti636G → W in BAA88522 (PubMed:10777669).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_064920147V → I.4 PublicationsCorresponds to variant rs7256349dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0069121 – 281Missing in isoform 4. 2 PublicationsAdd BLAST281
Alternative sequenceiVSP_0069101 – 105Missing in isoform 3. 1 PublicationAdd BLAST105
Alternative sequenceiVSP_00691155 – 86Missing in isoform 2. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_006913282 – 284LPE → MLQ in isoform 4. 2 Publications3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029149 mRNA. Translation: BAA88522.1.
AB029150 mRNA. Translation: BAA88523.1.
AF275680 mRNA. Translation: AAG24390.1.
AF275681 mRNA. Translation: AAG24391.1.
BX641169 mRNA. Translation: CAE46074.1.
BT007304 mRNA. Translation: AAP35968.1.
AC008751 Genomic DNA. No translation available.
AC020915 Genomic DNA. No translation available.
BC009763 mRNA. Translation: AAH09763.1.
U71598 mRNA. Translation: AAB16810.1.
CCDSiCCDS74473.1. [Q96GC6-1]
CCDS74474.1. [Q96GC6-2]
CCDS74476.1. [Q96GC6-3]
RefSeqiNP_001265663.1. NM_001278734.1.
NP_057408.2. NM_016324.3. [Q96GC6-3]
NP_057409.1. NM_016325.3. [Q96GC6-2]
NP_598009.1. NM_133502.2. [Q96GC6-1]
XP_011524629.1. XM_011526327.1. [Q96GC6-3]
XP_016881663.1. XM_017026174.1. [Q96GC6-3]
UniGeneiHs.83761.

Genome annotation databases

EnsembliENST00000345813; ENSP00000321187; ENSG00000171606. [Q96GC6-2]
ENST00000424679; ENSP00000409872; ENSG00000171606. [Q96GC6-3]
ENST00000610905; ENSP00000478533; ENSG00000171606. [Q96GC6-1]
ENST00000617501; ENSP00000484810; ENSG00000171606. [Q96GC6-1]
GeneIDi10782.
KEGGihsa:10782.
UCSCiuc032igq.2. human. [Q96GC6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029149 mRNA. Translation: BAA88522.1.
AB029150 mRNA. Translation: BAA88523.1.
AF275680 mRNA. Translation: AAG24390.1.
AF275681 mRNA. Translation: AAG24391.1.
BX641169 mRNA. Translation: CAE46074.1.
BT007304 mRNA. Translation: AAP35968.1.
AC008751 Genomic DNA. No translation available.
AC020915 Genomic DNA. No translation available.
BC009763 mRNA. Translation: AAH09763.1.
U71598 mRNA. Translation: AAB16810.1.
CCDSiCCDS74473.1. [Q96GC6-1]
CCDS74474.1. [Q96GC6-2]
CCDS74476.1. [Q96GC6-3]
RefSeqiNP_001265663.1. NM_001278734.1.
NP_057408.2. NM_016324.3. [Q96GC6-3]
NP_057409.1. NM_016325.3. [Q96GC6-2]
NP_598009.1. NM_133502.2. [Q96GC6-1]
XP_011524629.1. XM_011526327.1. [Q96GC6-3]
XP_016881663.1. XM_017026174.1. [Q96GC6-3]
UniGeneiHs.83761.

3D structure databases

ProteinModelPortaliQ96GC6.
SMRiQ96GC6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115999. 11 interactors.
IntActiQ96GC6. 2 interactors.
STRINGi9606.ENSP00000321209.

PTM databases

iPTMnetiQ96GC6.
PhosphoSitePlusiQ96GC6.

Polymorphism and mutation databases

BioMutaiZNF274.
DMDMi327478548.

Proteomic databases

MaxQBiQ96GC6.
PaxDbiQ96GC6.
PeptideAtlasiQ96GC6.
PRIDEiQ96GC6.

Protocols and materials databases

DNASUi10782.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000345813; ENSP00000321187; ENSG00000171606. [Q96GC6-2]
ENST00000424679; ENSP00000409872; ENSG00000171606. [Q96GC6-3]
ENST00000610905; ENSP00000478533; ENSG00000171606. [Q96GC6-1]
ENST00000617501; ENSP00000484810; ENSG00000171606. [Q96GC6-1]
GeneIDi10782.
KEGGihsa:10782.
UCSCiuc032igq.2. human. [Q96GC6-1]

Organism-specific databases

CTDi10782.
DisGeNETi10782.
GeneCardsiZNF274.
HGNCiHGNC:13068. ZNF274.
HPAiHPA028880.
MIMi605467. gene.
neXtProtiNX_Q96GC6.
OpenTargetsiENSG00000171606.
PharmGKBiPA37644.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00680000099963.
HOVERGENiHBG018163.
InParanoidiQ96GC6.
KOiK12458.
OMAiTAWSCEP.
OrthoDBiEOG091G02MB.
PhylomeDBiQ96GC6.
TreeFamiTF338018.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000171606-MONOMER.
ReactomeiR-HSA-212436. Generic Transcription Pathway.
SignaLinkiQ96GC6.

Miscellaneous databases

ChiTaRSiZNF274. human.
GeneWikiiZNF274.
GenomeRNAii10782.
PROiQ96GC6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171606.
CleanExiHS_ZNF274.
ExpressionAtlasiQ96GC6. baseline and differential.
GenevisibleiQ96GC6. HS.

Family and domain databases

CDDicd07765. KRAB_A-box. 2 hits.
Gene3Di3.30.160.60. 5 hits.
InterProiIPR001909. KRAB.
IPR008916. Retrov_capsid_C.
IPR003309. SCAN_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 2 hits.
PF02023. SCAN. 1 hit.
PF00096. zf-C2H2. 3 hits.
[Graphical view]
SMARTiSM00349. KRAB. 2 hits.
SM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 5 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 2 hits.
SSF47353. SSF47353. 1 hit.
PROSITEiPS50805. KRAB. 2 hits.
PS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZN274_HUMAN
AccessioniPrimary (citable) accession number: Q96GC6
Secondary accession number(s): Q53XU4
, Q6MZG1, Q8WY37, Q8WY38, Q92969, Q9UII0, Q9UII1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: April 5, 2011
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.