ID   S35A4_HUMAN             Reviewed;         324 AA.
AC   Q96G79; A8K013;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   24-JAN-2024, entry version 149.
DE   RecName: Full=Probable UDP-sugar transporter protein SLC35A4 {ECO:0000305};
DE   AltName: Full=Solute carrier family 35 member A4 {ECO:0000312|HGNC:HGNC:20753};
GN   Name=SLC35A4 {ECO:0000312|HGNC:HGNC:20753};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Adrenal gland;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Uterus;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Muscle;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   ALTERNATIVE INITIATION (ISOFORM 2).
RX   PubMed=25621764; DOI=10.7554/elife.03971;
RA   Andreev D.E., O'Connor P.B., Fahey C., Kenny E.M., Terenin I.M.,
RA   Dmitriev S.E., Cormican P., Morris D.W., Shatsky I.N., Baranov P.V.;
RT   "Translation of 5' leaders is pervasive in genes resistant to eIF2
RT   repression.";
RL   Elife 4:E03971-E03971(2015).
RN   [6]
RP   FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, AND IDENTIFICATION IN A COMPLEX
RP   WITH SLC35A2 AND SLC35A3.
RX   PubMed=28167211; DOI=10.1016/j.bbamcr.2017.02.002;
RA   Sosicka P., Maszczak-Seneczko D., Bazan B., Shauchuk Y., Kaczmarek B.,
RA   Olczak M.;
RT   "An insight into the orphan nucleotide sugar transporter SLC35A4.";
RL   Biochim. Biophys. Acta 1864:825-838(2017).
RN   [7]
RP   FUNCTION, AND TRANSPORTER ACTIVITY.
RX   PubMed=34015330; DOI=10.1016/j.jbc.2021.100789;
RA   Ury B., Potelle S., Caligiore F., Whorton M.R., Bommer G.T.;
RT   "The promiscuous binding pocket of SLC35A1 ensures redundant transport of
RT   CDP-ribitol to the Golgi.";
RL   J. Biol. Chem. 296:100789-100789(2021).
CC   -!- FUNCTION: Mediates the transport of CDP-ribitol (PubMed:34015330). Does
CC       not exhibit CMP-sialic acid, UDP-galactose and UDP-N-acetylglucosamine
CC       transport activity (PubMed:28167211, PubMed:34015330).
CC       {ECO:0000269|PubMed:28167211, ECO:0000269|PubMed:34015330}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=CDP(out) + CDP-L-ribitol(in) = CDP(in) + CDP-L-ribitol(out);
CC         Xref=Rhea:RHEA:71579, ChEBI:CHEBI:57608, ChEBI:CHEBI:58069;
CC         Evidence={ECO:0000269|PubMed:34015330};
CC   -!- SUBUNIT: Found in a complex with SLC35A2 and SLC35A3.
CC       {ECO:0000269|PubMed:28167211}.
CC   -!- INTERACTION:
CC       Q96G79; Q13323: BIK; NbExp=3; IntAct=EBI-12363689, EBI-700794;
CC       Q96G79; P21964: COMT; NbExp=3; IntAct=EBI-12363689, EBI-372265;
CC       Q96G79; Q7Z7G2: CPLX4; NbExp=3; IntAct=EBI-12363689, EBI-18013275;
CC       Q96G79; Q96BA8: CREB3L1; NbExp=3; IntAct=EBI-12363689, EBI-6942903;
CC       Q96G79; P00387: CYB5R3; NbExp=3; IntAct=EBI-12363689, EBI-1046040;
CC       Q96G79; O00559: EBAG9; NbExp=3; IntAct=EBI-12363689, EBI-8787095;
CC       Q96G79; Q15125: EBP; NbExp=3; IntAct=EBI-12363689, EBI-3915253;
CC       Q96G79; Q9Y282: ERGIC3; NbExp=3; IntAct=EBI-12363689, EBI-781551;
CC       Q96G79; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-12363689, EBI-18304435;
CC       Q96G79; A2A2Y4: FRMD3; NbExp=3; IntAct=EBI-12363689, EBI-6911547;
CC       Q96G79; Q9UJ14: GGT7; NbExp=3; IntAct=EBI-12363689, EBI-1058791;
CC       Q96G79; P48165: GJA8; NbExp=3; IntAct=EBI-12363689, EBI-17458373;
CC       Q96G79; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-12363689, EBI-13345167;
CC       Q96G79; Q9BSE4: HERPUD2; NbExp=3; IntAct=EBI-12363689, EBI-2868124;
CC       Q96G79; Q9NY72: SCN3B; NbExp=3; IntAct=EBI-12363689, EBI-17247926;
CC       Q96G79; Q96EP9: SLC10A4; NbExp=3; IntAct=EBI-12363689, EBI-17456472;
CC       Q96G79; Q9UKG4: SLC13A4; NbExp=3; IntAct=EBI-12363689, EBI-12808018;
CC       Q96G79; Q8WWF3: SSMEM1; NbExp=3; IntAct=EBI-12363689, EBI-17280858;
CC       Q96G79; Q8N9I0: SYT2; NbExp=3; IntAct=EBI-12363689, EBI-8032987;
CC       Q96G79; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-12363689, EBI-8638294;
CC       Q96G79; Q6P9G4: TMEM154; NbExp=3; IntAct=EBI-12363689, EBI-13329239;
CC       Q96G79; Q96Q45-2: TMEM237; NbExp=5; IntAct=EBI-12363689, EBI-10982110;
CC       Q96G79; Q4KMG9: TMEM52B; NbExp=3; IntAct=EBI-12363689, EBI-18178701;
CC       Q96G79; Q9BSR8: YIPF4; NbExp=3; IntAct=EBI-12363689, EBI-751253;
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000269|PubMed:28167211}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q96G79-1; Sequence=Displayed;
CC       Name=2; Synonyms=uORF {ECO:0000303|PubMed:25621764};
CC         IsoId=L0R6Q1-1; Sequence=External;
CC   -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35A
CC       subfamily. {ECO:0000305}.
CC   -!- CAUTION: The translation and the expression of this protein may be
CC       maintained under stress conditions thanks to the presence of the
CC       upstream ORF in transcripts. {ECO:0000269|PubMed:25621764}.
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DR   EMBL; AK289378; BAF82067.1; -; mRNA.
DR   EMBL; AL833972; CAD38817.1; -; mRNA.
DR   EMBL; CH471062; EAW62040.1; -; Genomic_DNA.
DR   EMBL; BC009906; AAH09906.1; -; mRNA.
DR   CCDS; CCDS4231.1; -. [Q96G79-1]
DR   RefSeq; NP_542401.1; NM_080670.3. [Q96G79-1]
DR   AlphaFoldDB; Q96G79; -.
DR   SMR; Q96G79; -.
DR   BioGRID; 125263; 33.
DR   IntAct; Q96G79; 25.
DR   MINT; Q96G79; -.
DR   STRING; 9606.ENSP00000424566; -.
DR   TCDB; 2.A.7.12.12; the drug/metabolite transporter (dmt) superfamily.
DR   GlyGen; Q96G79; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q96G79; -.
DR   PhosphoSitePlus; Q96G79; -.
DR   SwissPalm; Q96G79; -.
DR   BioMuta; SLC35A4; -.
DR   DMDM; 74731793; -.
DR   PaxDb; 9606-ENSP00000424566; -.
DR   PeptideAtlas; Q96G79; -.
DR   Antibodypedia; 45451; 28 antibodies from 13 providers.
DR   DNASU; 113829; -.
DR   Ensembl; ENST00000323146.8; ENSP00000327133.3; ENSG00000176087.16. [Q96G79-1]
DR   Ensembl; ENST00000514199.1; ENSP00000424566.1; ENSG00000176087.16. [Q96G79-1]
DR   Ensembl; ENST00000612662.2; ENSP00000479255.1; ENSG00000176087.16. [Q96G79-1]
DR   GeneID; 113829; -.
DR   KEGG; hsa:113829; -.
DR   MANE-Select; ENST00000323146.8; ENSP00000327133.3; NM_080670.4; NP_542401.1.
DR   UCSC; uc003lgg.1; human. [Q96G79-1]
DR   AGR; HGNC:20753; -.
DR   CTD; 113829; -.
DR   DisGeNET; 113829; -.
DR   GeneCards; SLC35A4; -.
DR   HGNC; HGNC:20753; SLC35A4.
DR   HPA; ENSG00000176087; Low tissue specificity.
DR   MIM; 620297; gene.
DR   neXtProt; NX_Q96G79; -.
DR   OpenTargets; ENSG00000176087; -.
DR   PharmGKB; PA134971555; -.
DR   VEuPathDB; HostDB:ENSG00000176087; -.
DR   eggNOG; KOG2234; Eukaryota.
DR   GeneTree; ENSGT00950000182827; -.
DR   HOGENOM; CLU_024645_5_1_1; -.
DR   InParanoid; Q96G79; -.
DR   OMA; SSCVVMI; -.
DR   OrthoDB; 4641608at2759; -.
DR   PhylomeDB; Q96G79; -.
DR   TreeFam; TF315345; -.
DR   PathwayCommons; Q96G79; -.
DR   SignaLink; Q96G79; -.
DR   BioGRID-ORCS; 113829; 11 hits in 1152 CRISPR screens.
DR   ChiTaRS; SLC35A4; human.
DR   GenomeRNAi; 113829; -.
DR   Pharos; Q96G79; Tdark.
DR   Proteomes; UP000005640; Chromosome 5.
DR   RNAct; Q96G79; Protein.
DR   Bgee; ENSG00000176087; Expressed in islet of Langerhans and 180 other cell types or tissues.
DR   ExpressionAtlas; Q96G79; baseline and differential.
DR   GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR   GO; GO:0000139; C:Golgi membrane; IDA:UniProtKB.
DR   GO; GO:0015165; F:pyrimidine nucleotide-sugar transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007271; Nuc_sug_transpt.
DR   PANTHER; PTHR10231; NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER; 1.
DR   PANTHER; PTHR10231:SF96; UDP-SUGAR TRANSPORTER PROTEIN SLC35A4-RELATED; 1.
DR   Pfam; PF04142; Nuc_sug_transp; 1.
DR   PIRSF; PIRSF005799; UDP-gal_transpt; 1.
DR   SUPFAM; SSF103481; Multidrug resistance efflux transporter EmrE; 1.
DR   Genevisible; Q96G79; HS.
PE   1: Evidence at protein level;
KW   Alternative initiation; Golgi apparatus; Membrane; Reference proteome;
KW   Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..324
FT                   /note="Probable UDP-sugar transporter protein SLC35A4"
FT                   /id="PRO_0000337748"
FT   TOPO_DOM        1..18
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:28167211"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..52
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305|PubMed:28167211"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        74..85
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:28167211"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        107..142
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305|PubMed:28167211"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        164..180
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:28167211"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        202..214
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305|PubMed:28167211"
FT   TRANSMEM        215..235
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        236..250
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:28167211"
FT   TRANSMEM        251..271
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        272..275
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305|PubMed:28167211"
FT   TRANSMEM        276..298
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        299..324
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:28167211"
FT   CONFLICT        79
FT                   /note="G -> E (in Ref. 1; BAF82067)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        81
FT                   /note="P -> L (in Ref. 1; BAF82067)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        306
FT                   /note="F -> L (in Ref. 1; BAF82067)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   324 AA;  34593 MW;  9BD54BA459B6C94A CRC64;
     MSVEDGGMPG LGRPRQARWT LMLLLSTAMY GAHAPLLALC HVDGRVPFRP SSAVLLTELT
     KLLLCAFSLL VGWQAWPQGP PPWRQAAPFA LSALLYGANN NLVIYLQRYM DPSTYQVLSN
     LKIGSTAVLY CLCLRHRLSV RQGLALLLLM AAGACYAAGG LQVPGNTLPS PPPAAAASPM
     PLHITPLGLL LLILYCLISG LSSVYTELLM KRQRLPLALQ NLFLYTFGVL LNLGLHAGGG
     SGPGLLEGFS GWAALVVLSQ ALNGLLMSAV MKHGSSITRL FVVSCSLVVN AVLSAVLLRL
     QLTAAFFLAT LLIGLAMRLY YGSR
//