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Protein

Diphosphoinositol polyphosphate phosphohydrolase 3-beta

Gene

NUDT11

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate.1 Publication

Catalytic activityi

Diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate.3 Publications
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-pentaphosphate + AMP.3 Publications
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP.3 Publications

Cofactori

Mg2+By similarity1 Publication, Mn2+By similarity1 PublicationNote: Binds 3 Mg2+ or Mn2+ ions per subunit. Mn2+ may be the true cofactor in vivo.By similarity1 Publication

Kineticsi

  1. KM=0.053 µM for PP-InsP52 Publications
  2. KM=13 µM for Ap6A2 Publications
  3. KM=37 µM for Ap5A2 Publications

    pH dependencei

    Optimum pH is 8.5.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei9SubstrateBy similarity1
    Binding sitei40SubstrateBy similarity1
    Metal bindingi49Magnesium 1; via carbonyl oxygenBy similarity1
    Metal bindingi65Magnesium 2By similarity1
    Metal bindingi65Magnesium 3By similarity1
    Active sitei68Proton acceptorBy similarity1
    Metal bindingi69Magnesium 1By similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS05381-MONOMER
    BRENDAi3.6.1.52 2681
    3.6.1.60 2681
    ReactomeiR-HSA-1855167 Synthesis of pyrophosphates in the cytosol

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diphosphoinositol polyphosphate phosphohydrolase 3-beta (EC:3.6.1.523 Publications)
    Short name:
    DIPP-3-beta
    Short name:
    DIPP3-beta
    Short name:
    hDIPP3beta
    Alternative name(s):
    Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-beta
    Diadenosine hexaphosphate hydrolase (AMP-forming) (EC:3.6.1.603 Publications)
    Nucleoside diphosphate-linked moiety X motif 11
    Short name:
    Nudix motif 11
    hAps1
    Gene namesi
    Name:NUDT11
    Synonyms:APS1, DIPP3B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome X

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000196368.4
    HGNCiHGNC:18011 NUDT11
    MIMi300528 gene
    neXtProtiNX_Q96G61

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi55190
    OpenTargetsiENSG00000196368
    PharmGKBiPA31832

    Polymorphism and mutation databases

    BioMutaiNUDT11
    DMDMi68565927

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000570651 – 164Diphosphoinositol polyphosphate phosphohydrolase 3-betaAdd BLAST164

    Proteomic databases

    EPDiQ96G61
    MaxQBiQ96G61
    PaxDbiQ96G61
    PeptideAtlasiQ96G61
    PRIDEiQ96G61

    PTM databases

    iPTMnetiQ96G61
    PhosphoSitePlusiQ96G61

    Expressioni

    Tissue specificityi

    Mainly expressed in testis and, at lower level in brain. According to PubMed:12121577, it is also expressed in pancreas and weakly expressed in thymus, prostate, ovary, lung, small intestine and heart.2 Publications

    Gene expression databases

    BgeeiENSG00000196368
    CleanExiHS_NUDT11
    GenevisibleiQ96G61 HS

    Organism-specific databases

    HPAiHPA047027
    HPA057684

    Interactioni

    Protein-protein interaction databases

    BioGridi120487, 1 interactor
    IntActiQ96G61, 1 interactor
    STRINGi9606.ENSP00000365160

    Structurei

    3D structure databases

    ProteinModelPortaliQ96G61
    SMRiQ96G61
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini17 – 144Nudix hydrolasePROSITE-ProRule annotationAdd BLAST128

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni17 – 19Substrate bindingBy similarity3
    Regioni89 – 91Substrate bindingBy similarity3

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi50 – 71Nudix boxAdd BLAST22

    Sequence similaritiesi

    Belongs to the Nudix hydrolase family. DIPP subfamily.Curated

    Phylogenomic databases

    eggNOGiKOG2839 Eukaryota
    COG0494 LUCA
    GeneTreeiENSGT00390000012928
    HOGENOMiHOG000237336
    HOVERGENiHBG053341
    InParanoidiQ96G61
    KOiK07766
    OMAiLQTWEEN
    OrthoDBiEOG091G0TH6
    PhylomeDBiQ96G61
    TreeFamiTF106349

    Family and domain databases

    InterProiView protein in InterPro
    IPR015797 NUDIX_hydrolase-like_dom_sf
    IPR020084 NUDIX_hydrolase_CS
    IPR000086 NUDIX_hydrolase_dom
    PfamiView protein in Pfam
    PF00293 NUDIX, 1 hit
    SUPFAMiSSF55811 SSF55811, 1 hit
    PROSITEiView protein in PROSITE
    PS51462 NUDIX, 1 hit
    PS00893 NUDIX_BOX, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q96G61-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKCKPNQTRT YDPEGFKKRA ACLCFRSERE DEVLLVSSSR YPDRWIVPGG
    60 70 80 90 100
    GMEPEEEPGG AAVREVYEEA GVKGKLGRLL GVFEQNQDRK HRTYVYVLTV
    110 120 130 140 150
    TELLEDWEDS VSIGRKREWF KVEDAIKVLQ CHKPVHAEYL EKLKLGGSPT
    160
    NGNSMAPSSP DSDP
    Length:164
    Mass (Da):18,559
    Last modified:December 1, 2001 - v1
    Checksum:i8E7F342C8A38872F
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_02273839S → N2 Publications1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AK001490 mRNA Translation: BAA91720.1
    AL158055 Genomic DNA No translation available.
    BC009942 mRNA Translation: AAH09942.1
    CCDSiCCDS43952.1
    RefSeqiNP_060629.2, NM_018159.3
    UniGeneiHs.200016

    Genome annotation databases

    EnsembliENST00000375992; ENSP00000365160; ENSG00000196368
    GeneIDi55190
    KEGGihsa:55190
    UCSCiuc010njt.4 human

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiNUD11_HUMAN
    AccessioniPrimary (citable) accession number: Q96G61
    Secondary accession number(s): Q9NVN0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: December 1, 2001
    Last modified: May 23, 2018
    This is version 151 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

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