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Protein

tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like

Gene

DUS3L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.By similarity

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei365FMNBy similarity1
Active sitei396Proton donorBy similarity1
Binding sitei435FMNBy similarity1
Binding sitei465FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri118 – 148C3H1-type 1PROSITE-ProRule annotationAdd BLAST31
Zinc fingeri156 – 186C3H1-type 2PROSITE-ProRule annotationAdd BLAST31
Nucleotide bindingi311 – 313FMNBy similarity3
Nucleotide bindingi497 – 499FMNBy similarity3
Nucleotide bindingi520 – 521FMNBy similarity2

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Biological processtRNA processing
LigandFlavoprotein, FMN, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like (EC:1.3.1.-)
Alternative name(s):
tRNA-dihydrouridine synthase 3-like
Gene namesi
Name:DUS3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000141994.15
HGNCiHGNC:26920 DUS3L
neXtProtiNX_Q96G46

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000141994
PharmGKBiPA142671938

Polymorphism and mutation databases

BioMutaiDUS3L
DMDMi110808212

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002473422 – 650tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-likeAdd BLAST649

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei236PhosphoserineCombined sources1
Modified residuei273PhosphothreonineCombined sources1
Modified residuei276PhosphoserineCombined sources1
Modified residuei277PhosphoserineBy similarity1
Cross-linki416Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96G46
MaxQBiQ96G46
PaxDbiQ96G46
PeptideAtlasiQ96G46
PRIDEiQ96G46

PTM databases

iPTMnetiQ96G46
PhosphoSitePlusiQ96G46

Expressioni

Gene expression databases

BgeeiENSG00000141994
CleanExiHS_DUS3L
ExpressionAtlasiQ96G46 baseline and differential
GenevisibleiQ96G46 HS

Organism-specific databases

HPAiHPA041854
HPA041944
HPA062846

Interactioni

Protein-protein interaction databases

BioGridi121258, 30 interactors
IntActiQ96G46, 1 interactor
STRINGi9606.ENSP00000311977

Structurei

3D structure databases

ProteinModelPortaliQ96G46
SMRiQ96G46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family. Dus3 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri118 – 148C3H1-type 1PROSITE-ProRule annotationAdd BLAST31
Zinc fingeri156 – 186C3H1-type 2PROSITE-ProRule annotationAdd BLAST31

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2333 Eukaryota
COG0042 LUCA
GeneTreeiENSGT00550000075134
HOGENOMiHOG000240610
HOVERGENiHBG062413
InParanoidiQ96G46
KOiK05544
OMAiWALMKAH
OrthoDBiEOG091G03H2
PhylomeDBiQ96G46
TreeFamiTF105726

Family and domain databases

CDDicd02801 DUS_like_FMN, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035587 DUS-like_FMN-bd
IPR001269 tRNA_hU_synthase
IPR018517 tRNA_hU_synthase_CS
IPR000571 Znf_CCCH
PANTHERiPTHR11082 PTHR11082, 1 hit
PfamiView protein in Pfam
PF01207 Dus, 1 hit
PROSITEiView protein in PROSITE
PS01136 UPF0034, 1 hit
PS50103 ZF_C3H1, 2 hits

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96G46-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEGTAEAPL ENGGGGDSGA GALERGVAPI KRQYLTTKEQ FHQFLEAKGQ
60 70 80 90 100
EKTCRETEVG DPAGNELAEP EAKRIRLEDG QTADGQTEEA AEPGEQLQTQ
110 120 130 140 150
KRARGQNKGR PHVKPTNYDK NRLCPSLIQE SAAKCFFGDR CRFLHDVGRY
160 170 180 190 200
LETKPADLGP RCVLFETFGR CPYGVTCRFA GAHLRPEGQN LVQEELAARG
210 220 230 240 250
TQPPSIRNGL DKALQQQLRK REVRFERAEQ ALRRFSQGPT PAAAVPEGTA
260 270 280 290 300
AEGAPRQENC GAQQVPAGPG TSTPPSSPVR TCGPLTDEDV VRLRPCEKKR
310 320 330 340 350
LDIRGKLYLA PLTTCGNLPF RRICKRFGAD VTCGEMAVCT NLLQGQMSEW
360 370 380 390 400
ALLKRHQCED IFGVQLEGAF PDTMTKCAEL LSRTVEVDFV DINVGCPIDL
410 420 430 440 450
VYKKGGGCAL MNRSTKFQQI VRGMNQVLDV PLTVKIRTGV QERVNLAHRL
460 470 480 490 500
LPELRDWGVA LVTLHGRSRE QRYTKLADWQ YIEECVQAAS PMPLFGNGDI
510 520 530 540 550
LSFEDANRAM QTGVTGIMIA RGALLKPWLF TEIKEQRHWD ISSSERLDIL
560 570 580 590 600
RDFTNYGLEH WGSDTQGVEK TRRFLLEWLS FLCRYVPVGL LERLPQRINE
610 620 630 640 650
RPPYYLGRDY LETLMASQKA ADWIRISEML LGPVPPSFAF LPKHKANAYK
Length:650
Mass (Da):72,594
Last modified:July 25, 2006 - v2
Checksum:iB785373796AB88E0
GO
Isoform 2 (identifier: Q96G46-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     464-482: LHGRSREQRYTKLADWQYI → VGLGTPRALGPRGRPRVPS
     483-650: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:482
Mass (Da):52,772
Checksum:iD5E6DB1B7E8827E3
GO
Isoform 3 (identifier: Q96G46-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-300: Missing.

Note: No experimental confirmation available.
Show »
Length:408
Mass (Da):46,106
Checksum:i96931E7C0C377C89
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134K → N in BAB14740 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027092185R → G1 PublicationCorresponds to variant dbSNP:rs2436487Ensembl.1
Natural variantiVAR_027093609D → N. Corresponds to variant dbSNP:rs12977803Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01997059 – 300Missing in isoform 3. 1 PublicationAdd BLAST242
Alternative sequenceiVSP_019971464 – 482LHGRS…DWQYI → VGLGTPRALGPRGRPRVPS in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_019972483 – 650Missing in isoform 2. 1 PublicationAdd BLAST168

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023958 mRNA Translation: BAB14740.1
AC011499 Genomic DNA No translation available.
BC004549 mRNA Translation: AAH04549.1
BC008362 mRNA Translation: AAH08362.1
BC009973 mRNA Translation: AAH09973.1
AL365411 mRNA Translation: CAB96955.1
CCDSiCCDS32880.1 [Q96G46-1]
CCDS54202.1 [Q96G46-3]
RefSeqiNP_001155091.1, NM_001161619.1 [Q96G46-3]
NP_064560.2, NM_020175.2 [Q96G46-1]
UniGeneiHs.284297

Genome annotation databases

EnsembliENST00000309061; ENSP00000311977; ENSG00000141994 [Q96G46-1]
ENST00000320699; ENSP00000315558; ENSG00000141994 [Q96G46-3]
GeneIDi56931
KEGGihsa:56931
UCSCiuc002mdc.4 human [Q96G46-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDUS3L_HUMAN
AccessioniPrimary (citable) accession number: Q96G46
Secondary accession number(s): Q96HM5
, Q9BSU4, Q9H877, Q9NPR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: April 25, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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