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Q96G46

- DUS3L_HUMAN

UniProt

Q96G46 - DUS3L_HUMAN

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Protein

tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like

Gene

DUS3L

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.By similarity

Cofactori

FAD.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri118 – 14831C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri156 – 18631C3H1-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. poly(A) RNA binding Source: UniProtKB
  4. tRNA dihydrouridine synthase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like (EC:1.3.1.-)
Alternative name(s):
tRNA-dihydrouridine synthase 3-like
Gene namesi
Name:DUS3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:26920. DUS3L.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671938.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 650649tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-likePRO_0000247342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine2 Publications
Modified residuei236 – 2361Phosphoserine3 Publications
Modified residuei273 – 2731Phosphothreonine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ96G46.
PaxDbiQ96G46.
PRIDEiQ96G46.

PTM databases

PhosphoSiteiQ96G46.

Expressioni

Gene expression databases

BgeeiQ96G46.
CleanExiHS_DUS3L.
ExpressionAtlasiQ96G46. baseline and differential.
GenevestigatoriQ96G46.

Organism-specific databases

HPAiHPA041854.
HPA041944.

Interactioni

Protein-protein interaction databases

BioGridi121258. 19 interactions.
IntActiQ96G46. 1 interaction.
STRINGi9606.ENSP00000311977.

Structurei

3D structure databases

ProteinModelPortaliQ96G46.
SMRiQ96G46. Positions 306-575.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family. Dus3 subfamily.Curated
Contains 2 C3H1-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri118 – 14831C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri156 – 18631C3H1-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0042.
GeneTreeiENSGT00550000075134.
HOGENOMiHOG000240610.
HOVERGENiHBG062413.
InParanoidiQ96G46.
KOiK05544.
OMAiESAAKCF.
OrthoDBiEOG76HQ12.
PhylomeDBiQ96G46.
TreeFamiTF105726.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
IPR000571. Znf_CCCH.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PROSITEiPS01136. UPF0034. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96G46-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEGTAEAPL ENGGGGDSGA GALERGVAPI KRQYLTTKEQ FHQFLEAKGQ
60 70 80 90 100
EKTCRETEVG DPAGNELAEP EAKRIRLEDG QTADGQTEEA AEPGEQLQTQ
110 120 130 140 150
KRARGQNKGR PHVKPTNYDK NRLCPSLIQE SAAKCFFGDR CRFLHDVGRY
160 170 180 190 200
LETKPADLGP RCVLFETFGR CPYGVTCRFA GAHLRPEGQN LVQEELAARG
210 220 230 240 250
TQPPSIRNGL DKALQQQLRK REVRFERAEQ ALRRFSQGPT PAAAVPEGTA
260 270 280 290 300
AEGAPRQENC GAQQVPAGPG TSTPPSSPVR TCGPLTDEDV VRLRPCEKKR
310 320 330 340 350
LDIRGKLYLA PLTTCGNLPF RRICKRFGAD VTCGEMAVCT NLLQGQMSEW
360 370 380 390 400
ALLKRHQCED IFGVQLEGAF PDTMTKCAEL LSRTVEVDFV DINVGCPIDL
410 420 430 440 450
VYKKGGGCAL MNRSTKFQQI VRGMNQVLDV PLTVKIRTGV QERVNLAHRL
460 470 480 490 500
LPELRDWGVA LVTLHGRSRE QRYTKLADWQ YIEECVQAAS PMPLFGNGDI
510 520 530 540 550
LSFEDANRAM QTGVTGIMIA RGALLKPWLF TEIKEQRHWD ISSSERLDIL
560 570 580 590 600
RDFTNYGLEH WGSDTQGVEK TRRFLLEWLS FLCRYVPVGL LERLPQRINE
610 620 630 640 650
RPPYYLGRDY LETLMASQKA ADWIRISEML LGPVPPSFAF LPKHKANAYK
Length:650
Mass (Da):72,594
Last modified:July 25, 2006 - v2
Checksum:iB785373796AB88E0
GO
Isoform 2 (identifier: Q96G46-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     464-482: LHGRSREQRYTKLADWQYI → VGLGTPRALGPRGRPRVPS
     483-650: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:482
Mass (Da):52,772
Checksum:iD5E6DB1B7E8827E3
GO
Isoform 3 (identifier: Q96G46-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-300: Missing.

Note: No experimental confirmation available.

Show »
Length:408
Mass (Da):46,106
Checksum:i96931E7C0C377C89
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti134 – 1341K → N in BAB14740. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti185 – 1851R → G.1 Publication
Corresponds to variant rs2436487 [ dbSNP | Ensembl ].
VAR_027092
Natural varianti609 – 6091D → N.
Corresponds to variant rs12977803 [ dbSNP | Ensembl ].
VAR_027093

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei59 – 300242Missing in isoform 3. 1 PublicationVSP_019970Add
BLAST
Alternative sequencei464 – 48219LHGRS…DWQYI → VGLGTPRALGPRGRPRVPS in isoform 2. 1 PublicationVSP_019971Add
BLAST
Alternative sequencei483 – 650168Missing in isoform 2. 1 PublicationVSP_019972Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK023958 mRNA. Translation: BAB14740.1.
AC011499 Genomic DNA. No translation available.
BC004549 mRNA. Translation: AAH04549.1.
BC008362 mRNA. Translation: AAH08362.1.
BC009973 mRNA. Translation: AAH09973.1.
AL365411 mRNA. Translation: CAB96955.1.
CCDSiCCDS32880.1. [Q96G46-1]
CCDS54202.1. [Q96G46-3]
RefSeqiNP_001155091.1. NM_001161619.1. [Q96G46-3]
NP_064560.2. NM_020175.2. [Q96G46-1]
UniGeneiHs.284297.

Genome annotation databases

EnsembliENST00000309061; ENSP00000311977; ENSG00000141994. [Q96G46-1]
ENST00000320699; ENSP00000315558; ENSG00000141994. [Q96G46-3]
GeneIDi56931.
KEGGihsa:56931.
UCSCiuc002mdc.3. human. [Q96G46-1]
uc002mdd.3. human. [Q96G46-3]

Polymorphism databases

DMDMi110808212.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK023958 mRNA. Translation: BAB14740.1 .
AC011499 Genomic DNA. No translation available.
BC004549 mRNA. Translation: AAH04549.1 .
BC008362 mRNA. Translation: AAH08362.1 .
BC009973 mRNA. Translation: AAH09973.1 .
AL365411 mRNA. Translation: CAB96955.1 .
CCDSi CCDS32880.1. [Q96G46-1 ]
CCDS54202.1. [Q96G46-3 ]
RefSeqi NP_001155091.1. NM_001161619.1. [Q96G46-3 ]
NP_064560.2. NM_020175.2. [Q96G46-1 ]
UniGenei Hs.284297.

3D structure databases

ProteinModelPortali Q96G46.
SMRi Q96G46. Positions 306-575.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121258. 19 interactions.
IntActi Q96G46. 1 interaction.
STRINGi 9606.ENSP00000311977.

PTM databases

PhosphoSitei Q96G46.

Polymorphism databases

DMDMi 110808212.

Proteomic databases

MaxQBi Q96G46.
PaxDbi Q96G46.
PRIDEi Q96G46.

Protocols and materials databases

DNASUi 56931.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000309061 ; ENSP00000311977 ; ENSG00000141994 . [Q96G46-1 ]
ENST00000320699 ; ENSP00000315558 ; ENSG00000141994 . [Q96G46-3 ]
GeneIDi 56931.
KEGGi hsa:56931.
UCSCi uc002mdc.3. human. [Q96G46-1 ]
uc002mdd.3. human. [Q96G46-3 ]

Organism-specific databases

CTDi 56931.
GeneCardsi GC19M005737.
HGNCi HGNC:26920. DUS3L.
HPAi HPA041854.
HPA041944.
neXtProti NX_Q96G46.
PharmGKBi PA142671938.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0042.
GeneTreei ENSGT00550000075134.
HOGENOMi HOG000240610.
HOVERGENi HBG062413.
InParanoidi Q96G46.
KOi K05544.
OMAi ESAAKCF.
OrthoDBi EOG76HQ12.
PhylomeDBi Q96G46.
TreeFami TF105726.

Miscellaneous databases

GenomeRNAii 56931.
NextBioi 62471.
PROi Q96G46.

Gene expression databases

Bgeei Q96G46.
CleanExi HS_DUS3L.
ExpressionAtlasi Q96G46. baseline and differential.
Genevestigatori Q96G46.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
IPR000571. Znf_CCCH.
[Graphical view ]
PANTHERi PTHR11082. PTHR11082. 1 hit.
Pfami PF01207. Dus. 1 hit.
[Graphical view ]
PROSITEi PS01136. UPF0034. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Thyroid.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT GLY-185.
    Tissue: Pancreas, Placenta and Uterus.
  4. The European IMAGE consortium
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-650 (ISOFORM 2).
  5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-273, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDUS3L_HUMAN
AccessioniPrimary (citable) accession number: Q96G46
Secondary accession number(s): Q96HM5
, Q9BSU4, Q9H877, Q9NPR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: October 29, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3