Q96G46 (DUS3L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like
EC=1.3.1.-

Alternative name(s):
tRNA-dihydrouridine synthase 3-like

Gene names

Name:

DUS3L

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs By similarity.
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the dus family. Dus3 subfamily. Contains 2 C3H1-type zinc fingers.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	FAD Flavoprotein Metal-binding Zinc
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA dihydrouridine synthesis Inferred from electronic annotation. Source: GOC
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA dihydrouridine synthase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96G46-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96G46-2)

The sequence of this isoform differs from the canonical sequence as follows:
     464-482: LHGRSREQRYTKLADWQYI → VGLGTPRALGPRGRPRVPS
     483-650: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 3 (identifier: Q96G46-3)

The sequence of this isoform differs from the canonical sequence as follows:
     59-300: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like		PRO_0000247342
Regions
	Zinc finger	118 – 148	31	C3H1-type 1
	Zinc finger	156 – 186	31	C3H1-type 2
Amino acid modifications
	Modified residue	236	1	Phosphoserine Ref.7 Ref.8 Ref.10
	Modified residue	273	1	Phosphothreonine Ref.9
Natural variations
	Alternative sequence	59 – 300	242	Missing in isoform 3.		VSP_019970
	Alternative sequence	464 – 482	19	LHGRS…DWQYI → VGLGTPRALGPRGRPRVPS in isoform 2.		VSP_019971
	Alternative sequence	483 – 650	168	Missing in isoform 2.		VSP_019972
	Natural variant	185	1	R → G. Ref.3 Corresponds to variant rs2436487 [ dbSNP | Ensembl ].		VAR_027092
	Natural variant	609	1	D → N. Corresponds to variant rs12977803 [ dbSNP | Ensembl ].		VAR_027093
Experimental info
	Sequence conflict	134	1	K → N in BAB14740. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: B785373796AB88E0	FASTA	650	72,594
	10 20 30 40 50 60 MAEGTAEAPL ENGGGGDSGA GALERGVAPI KRQYLTTKEQ FHQFLEAKGQ EKTCRETEVG 70 80 90 100 110 120 DPAGNELAEP EAKRIRLEDG QTADGQTEEA AEPGEQLQTQ KRARGQNKGR PHVKPTNYDK 130 140 150 160 170 180 NRLCPSLIQE SAAKCFFGDR CRFLHDVGRY LETKPADLGP RCVLFETFGR CPYGVTCRFA 190 200 210 220 230 240 GAHLRPEGQN LVQEELAARG TQPPSIRNGL DKALQQQLRK REVRFERAEQ ALRRFSQGPT 250 260 270 280 290 300 PAAAVPEGTA AEGAPRQENC GAQQVPAGPG TSTPPSSPVR TCGPLTDEDV VRLRPCEKKR 310 320 330 340 350 360 LDIRGKLYLA PLTTCGNLPF RRICKRFGAD VTCGEMAVCT NLLQGQMSEW ALLKRHQCED 370 380 390 400 410 420 IFGVQLEGAF PDTMTKCAEL LSRTVEVDFV DINVGCPIDL VYKKGGGCAL MNRSTKFQQI 430 440 450 460 470 480 VRGMNQVLDV PLTVKIRTGV QERVNLAHRL LPELRDWGVA LVTLHGRSRE QRYTKLADWQ 490 500 510 520 530 540 YIEECVQAAS PMPLFGNGDI LSFEDANRAM QTGVTGIMIA RGALLKPWLF TEIKEQRHWD 550 560 570 580 590 600 ISSSERLDIL RDFTNYGLEH WGSDTQGVEK TRRFLLEWLS FLCRYVPVGL LERLPQRINE 610 620 630 640 650 RPPYYLGRDY LETLMASQKA ADWIRISEML LGPVPPSFAF LPKHKANAYK
	Isoform 2 [UniParc]. Checksum: D5E6DB1B7E8827E3	FASTA	482	52,772
	10 20 30 40 50 60 MAEGTAEAPL ENGGGGDSGA GALERGVAPI KRQYLTTKEQ FHQFLEAKGQ EKTCRETEVG 70 80 90 100 110 120 DPAGNELAEP EAKRIRLEDG QTADGQTEEA AEPGEQLQTQ KRARGQNKGR PHVKPTNYDK 130 140 150 160 170 180 NRLCPSLIQE SAAKCFFGDR CRFLHDVGRY LETKPADLGP RCVLFETFGR CPYGVTCRFA 190 200 210 220 230 240 GAHLRPEGQN LVQEELAARG TQPPSIRNGL DKALQQQLRK REVRFERAEQ ALRRFSQGPT 250 260 270 280 290 300 PAAAVPEGTA AEGAPRQENC GAQQVPAGPG TSTPPSSPVR TCGPLTDEDV VRLRPCEKKR 310 320 330 340 350 360 LDIRGKLYLA PLTTCGNLPF RRICKRFGAD VTCGEMAVCT NLLQGQMSEW ALLKRHQCED 370 380 390 400 410 420 IFGVQLEGAF PDTMTKCAEL LSRTVEVDFV DINVGCPIDL VYKKGGGCAL MNRSTKFQQI 430 440 450 460 470 480 VRGMNQVLDV PLTVKIRTGV QERVNLAHRL LPELRDWGVA LVTVGLGTPR ALGPRGRPRV PS
	Isoform 3 [UniParc]. Checksum: 96931E7C0C377C89	FASTA	408	46,106
	10 20 30 40 50 60 MAEGTAEAPL ENGGGGDSGA GALERGVAPI KRQYLTTKEQ FHQFLEAKGQ EKTCRETELD 70 80 90 100 110 120 IRGKLYLAPL TTCGNLPFRR ICKRFGADVT CGEMAVCTNL LQGQMSEWAL LKRHQCEDIF 130 140 150 160 170 180 GVQLEGAFPD TMTKCAELLS RTVEVDFVDI NVGCPIDLVY KKGGGCALMN RSTKFQQIVR 190 200 210 220 230 240 GMNQVLDVPL TVKIRTGVQE RVNLAHRLLP ELRDWGVALV TLHGRSREQR YTKLADWQYI 250 260 270 280 290 300 EECVQAASPM PLFGNGDILS FEDANRAMQT GVTGIMIARG ALLKPWLFTE IKEQRHWDIS 310 320 330 340 350 360 SSERLDILRD FTNYGLEHWG SDTQGVEKTR RFLLEWLSFL CRYVPVGLLE RLPQRINERP 370 380 390 400 PYYLGRDYLE TLMASQKAAD WIRISEMLLG PVPPSFAFLP KHKANAYK

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Thyroid.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT GLY-185. Tissue: Pancreas, Placenta and Uterus.
[4]	The European IMAGE consortium Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-650 (ISOFORM 2).
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-273, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK023958 mRNA. Translation: BAB14740.1. AC011499 Genomic DNA. No translation available. BC004549 mRNA. Translation: AAH04549.1. BC008362 mRNA. Translation: AAH08362.1. BC009973 mRNA. Translation: AAH09973.1. AL365411 mRNA. Translation: CAB96955.1.
IPI	IPI00186125. IPI00640947. IPI00784107.
RefSeq	NP_001155091.1. NM_001161619.1. NP_064560.2. NM_020175.2.
UniGene	Hs.284297.
3D structure databases
ProteinModelPortal	Q96G46.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96G46. 1 interaction.
STRING	9606.ENSP00000311977.
PTM databases
PhosphoSite	Q96G46.
Polymorphism databases
DMDM	110808212.
Proteomic databases
PaxDb	Q96G46.
PRIDE	Q96G46.
Protocols and materials databases
DNASU	56931.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000309061; ENSP00000311977; ENSG00000141994. ENST00000320699; ENSP00000315558; ENSG00000141994.
GeneID	56931.
KEGG	hsa:56931.
UCSC	uc002mdc.3. human. uc002mdd.3. human.
Organism-specific databases
CTD	56931.
GeneCards	GC19M005737.
HGNC	HGNC:26920. DUS3L.
HPA	HPA041854. HPA041944.
neXtProt	NX_Q96G46.
PharmGKB	PA142671938.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0042.
HOGENOM	HOG000240610.
HOVERGEN	HBG062413.
InParanoid	Q96G46.
KO	K05544.
OMA	MSEWALL.
OrthoDB	EOG4K6G43.
Gene expression databases
ArrayExpress	Q96G46.
Bgee	Q96G46.
CleanEx	HS_DUS3L.
Genevestigator	Q96G46.
GermOnline	ENSG00000141994. Homo sapiens.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001269. tRNA_hU_synthase. IPR018517. tRNA_hU_synthase_CS. IPR000571. Znf_CCCH. [Graphical view]
PANTHER	PTHR11082. PTHR11082. 1 hit.
Pfam	PF01207. Dus. 1 hit. [Graphical view]
PROSITE	PS01136. UPF0034. 1 hit. PS50103. ZF_C3H1. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	56931.
NextBio	62471.

Entry information

Entry name

DUS3L_HUMAN

Accession

Primary (citable) accession number: Q96G46
Secondary accession number(s): Q96HM5 , Q9BSU4, Q9H877, Q9NPR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.