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Protein

tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like

Gene

DUS3L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.By similarity

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei365FMNBy similarity1
Active sitei396Proton donorBy similarity1
Binding sitei435FMNBy similarity1
Binding sitei465FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri118 – 148C3H1-type 1PROSITE-ProRule annotationAdd BLAST31
Zinc fingeri156 – 186C3H1-type 2PROSITE-ProRule annotationAdd BLAST31
Nucleotide bindingi311 – 313FMNBy similarity3
Nucleotide bindingi497 – 499FMNBy similarity3
Nucleotide bindingi520 – 521FMNBy similarity2

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Flavoprotein, FMN, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like (EC:1.3.1.-)
Alternative name(s):
tRNA-dihydrouridine synthase 3-like
Gene namesi
Name:DUS3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:26920. DUS3L.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000141994.
PharmGKBiPA142671938.

Polymorphism and mutation databases

BioMutaiDUS3L.
DMDMi110808212.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002473422 – 650tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-likeAdd BLAST649

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei236PhosphoserineCombined sources1
Modified residuei273PhosphothreonineCombined sources1
Modified residuei276PhosphoserineCombined sources1
Modified residuei277PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96G46.
MaxQBiQ96G46.
PaxDbiQ96G46.
PeptideAtlasiQ96G46.
PRIDEiQ96G46.

PTM databases

iPTMnetiQ96G46.
PhosphoSitePlusiQ96G46.

Expressioni

Gene expression databases

BgeeiENSG00000141994.
CleanExiHS_DUS3L.
ExpressionAtlasiQ96G46. baseline and differential.
GenevisibleiQ96G46. HS.

Organism-specific databases

HPAiHPA041854.
HPA041944.
HPA062846.

Interactioni

Protein-protein interaction databases

BioGridi121258. 26 interactors.
IntActiQ96G46. 1 interactor.
STRINGi9606.ENSP00000311977.

Structurei

3D structure databases

ProteinModelPortaliQ96G46.
SMRiQ96G46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family. Dus3 subfamily.Curated
Contains 2 C3H1-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri118 – 148C3H1-type 1PROSITE-ProRule annotationAdd BLAST31
Zinc fingeri156 – 186C3H1-type 2PROSITE-ProRule annotationAdd BLAST31

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2333. Eukaryota.
COG0042. LUCA.
GeneTreeiENSGT00550000075134.
HOGENOMiHOG000240610.
HOVERGENiHBG062413.
InParanoidiQ96G46.
KOiK05544.
OMAiYIEECVQ.
OrthoDBiEOG091G03H2.
PhylomeDBiQ96G46.
TreeFamiTF105726.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
4.10.1000.10. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
IPR000571. Znf_CCCH.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PROSITEiPS01136. UPF0034. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96G46-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEGTAEAPL ENGGGGDSGA GALERGVAPI KRQYLTTKEQ FHQFLEAKGQ
60 70 80 90 100
EKTCRETEVG DPAGNELAEP EAKRIRLEDG QTADGQTEEA AEPGEQLQTQ
110 120 130 140 150
KRARGQNKGR PHVKPTNYDK NRLCPSLIQE SAAKCFFGDR CRFLHDVGRY
160 170 180 190 200
LETKPADLGP RCVLFETFGR CPYGVTCRFA GAHLRPEGQN LVQEELAARG
210 220 230 240 250
TQPPSIRNGL DKALQQQLRK REVRFERAEQ ALRRFSQGPT PAAAVPEGTA
260 270 280 290 300
AEGAPRQENC GAQQVPAGPG TSTPPSSPVR TCGPLTDEDV VRLRPCEKKR
310 320 330 340 350
LDIRGKLYLA PLTTCGNLPF RRICKRFGAD VTCGEMAVCT NLLQGQMSEW
360 370 380 390 400
ALLKRHQCED IFGVQLEGAF PDTMTKCAEL LSRTVEVDFV DINVGCPIDL
410 420 430 440 450
VYKKGGGCAL MNRSTKFQQI VRGMNQVLDV PLTVKIRTGV QERVNLAHRL
460 470 480 490 500
LPELRDWGVA LVTLHGRSRE QRYTKLADWQ YIEECVQAAS PMPLFGNGDI
510 520 530 540 550
LSFEDANRAM QTGVTGIMIA RGALLKPWLF TEIKEQRHWD ISSSERLDIL
560 570 580 590 600
RDFTNYGLEH WGSDTQGVEK TRRFLLEWLS FLCRYVPVGL LERLPQRINE
610 620 630 640 650
RPPYYLGRDY LETLMASQKA ADWIRISEML LGPVPPSFAF LPKHKANAYK
Length:650
Mass (Da):72,594
Last modified:July 25, 2006 - v2
Checksum:iB785373796AB88E0
GO
Isoform 2 (identifier: Q96G46-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     464-482: LHGRSREQRYTKLADWQYI → VGLGTPRALGPRGRPRVPS
     483-650: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:482
Mass (Da):52,772
Checksum:iD5E6DB1B7E8827E3
GO
Isoform 3 (identifier: Q96G46-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-300: Missing.

Note: No experimental confirmation available.
Show »
Length:408
Mass (Da):46,106
Checksum:i96931E7C0C377C89
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134K → N in BAB14740 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027092185R → G.1 PublicationCorresponds to variant rs2436487dbSNPEnsembl.1
Natural variantiVAR_027093609D → N.Corresponds to variant rs12977803dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01997059 – 300Missing in isoform 3. 1 PublicationAdd BLAST242
Alternative sequenceiVSP_019971464 – 482LHGRS…DWQYI → VGLGTPRALGPRGRPRVPS in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_019972483 – 650Missing in isoform 2. 1 PublicationAdd BLAST168

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023958 mRNA. Translation: BAB14740.1.
AC011499 Genomic DNA. No translation available.
BC004549 mRNA. Translation: AAH04549.1.
BC008362 mRNA. Translation: AAH08362.1.
BC009973 mRNA. Translation: AAH09973.1.
AL365411 mRNA. Translation: CAB96955.1.
CCDSiCCDS32880.1. [Q96G46-1]
CCDS54202.1. [Q96G46-3]
RefSeqiNP_001155091.1. NM_001161619.1. [Q96G46-3]
NP_064560.2. NM_020175.2. [Q96G46-1]
UniGeneiHs.284297.

Genome annotation databases

EnsembliENST00000309061; ENSP00000311977; ENSG00000141994. [Q96G46-1]
ENST00000320699; ENSP00000315558; ENSG00000141994. [Q96G46-3]
GeneIDi56931.
KEGGihsa:56931.
UCSCiuc002mdc.4. human. [Q96G46-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023958 mRNA. Translation: BAB14740.1.
AC011499 Genomic DNA. No translation available.
BC004549 mRNA. Translation: AAH04549.1.
BC008362 mRNA. Translation: AAH08362.1.
BC009973 mRNA. Translation: AAH09973.1.
AL365411 mRNA. Translation: CAB96955.1.
CCDSiCCDS32880.1. [Q96G46-1]
CCDS54202.1. [Q96G46-3]
RefSeqiNP_001155091.1. NM_001161619.1. [Q96G46-3]
NP_064560.2. NM_020175.2. [Q96G46-1]
UniGeneiHs.284297.

3D structure databases

ProteinModelPortaliQ96G46.
SMRiQ96G46.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121258. 26 interactors.
IntActiQ96G46. 1 interactor.
STRINGi9606.ENSP00000311977.

PTM databases

iPTMnetiQ96G46.
PhosphoSitePlusiQ96G46.

Polymorphism and mutation databases

BioMutaiDUS3L.
DMDMi110808212.

Proteomic databases

EPDiQ96G46.
MaxQBiQ96G46.
PaxDbiQ96G46.
PeptideAtlasiQ96G46.
PRIDEiQ96G46.

Protocols and materials databases

DNASUi56931.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309061; ENSP00000311977; ENSG00000141994. [Q96G46-1]
ENST00000320699; ENSP00000315558; ENSG00000141994. [Q96G46-3]
GeneIDi56931.
KEGGihsa:56931.
UCSCiuc002mdc.4. human. [Q96G46-1]

Organism-specific databases

CTDi56931.
GeneCardsiDUS3L.
HGNCiHGNC:26920. DUS3L.
HPAiHPA041854.
HPA041944.
HPA062846.
neXtProtiNX_Q96G46.
OpenTargetsiENSG00000141994.
PharmGKBiPA142671938.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2333. Eukaryota.
COG0042. LUCA.
GeneTreeiENSGT00550000075134.
HOGENOMiHOG000240610.
HOVERGENiHBG062413.
InParanoidiQ96G46.
KOiK05544.
OMAiYIEECVQ.
OrthoDBiEOG091G03H2.
PhylomeDBiQ96G46.
TreeFamiTF105726.

Miscellaneous databases

GenomeRNAii56931.
PROiQ96G46.

Gene expression databases

BgeeiENSG00000141994.
CleanExiHS_DUS3L.
ExpressionAtlasiQ96G46. baseline and differential.
GenevisibleiQ96G46. HS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
4.10.1000.10. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
IPR000571. Znf_CCCH.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PROSITEiPS01136. UPF0034. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS3L_HUMAN
AccessioniPrimary (citable) accession number: Q96G46
Secondary accession number(s): Q96HM5
, Q9BSU4, Q9H877, Q9NPR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.