Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q96G46 (DUS3L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like

EC=1.3.1.-
Alternative name(s):
tRNA-dihydrouridine synthase 3-like
Gene names
Name:DUS3L
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length650 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs By similarity.

Cofactor

FAD By similarity.

Sequence similarities

Belongs to the Dus family. Dus3 subfamily.

Contains 2 C3H1-type zinc fingers.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96G46-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96G46-2)

The sequence of this isoform differs from the canonical sequence as follows:
     464-482: LHGRSREQRYTKLADWQYI → VGLGTPRALGPRGRPRVPS
     483-650: Missing.
Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 3 (identifier: Q96G46-3)

The sequence of this isoform differs from the canonical sequence as follows:
     59-300: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 650649tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like
PRO_0000247342

Regions

Zinc finger118 – 14831C3H1-type 1
Zinc finger156 – 18631C3H1-type 2

Amino acid modifications

Modified residue21N-acetylalanine Ref.9 Ref.13
Modified residue2361Phosphoserine Ref.7 Ref.8 Ref.11
Modified residue2731Phosphothreonine Ref.10

Natural variations

Alternative sequence59 – 300242Missing in isoform 3.
VSP_019970
Alternative sequence464 – 48219LHGRS…DWQYI → VGLGTPRALGPRGRPRVPS in isoform 2.
VSP_019971
Alternative sequence483 – 650168Missing in isoform 2.
VSP_019972
Natural variant1851R → G. Ref.3
Corresponds to variant rs2436487 [ dbSNP | Ensembl ].
VAR_027092
Natural variant6091D → N.
Corresponds to variant rs12977803 [ dbSNP | Ensembl ].
VAR_027093

Experimental info

Sequence conflict1341K → N in BAB14740. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: B785373796AB88E0

FASTA65072,594
        10         20         30         40         50         60 
MAEGTAEAPL ENGGGGDSGA GALERGVAPI KRQYLTTKEQ FHQFLEAKGQ EKTCRETEVG 

        70         80         90        100        110        120 
DPAGNELAEP EAKRIRLEDG QTADGQTEEA AEPGEQLQTQ KRARGQNKGR PHVKPTNYDK 

       130        140        150        160        170        180 
NRLCPSLIQE SAAKCFFGDR CRFLHDVGRY LETKPADLGP RCVLFETFGR CPYGVTCRFA 

       190        200        210        220        230        240 
GAHLRPEGQN LVQEELAARG TQPPSIRNGL DKALQQQLRK REVRFERAEQ ALRRFSQGPT 

       250        260        270        280        290        300 
PAAAVPEGTA AEGAPRQENC GAQQVPAGPG TSTPPSSPVR TCGPLTDEDV VRLRPCEKKR 

       310        320        330        340        350        360 
LDIRGKLYLA PLTTCGNLPF RRICKRFGAD VTCGEMAVCT NLLQGQMSEW ALLKRHQCED 

       370        380        390        400        410        420 
IFGVQLEGAF PDTMTKCAEL LSRTVEVDFV DINVGCPIDL VYKKGGGCAL MNRSTKFQQI 

       430        440        450        460        470        480 
VRGMNQVLDV PLTVKIRTGV QERVNLAHRL LPELRDWGVA LVTLHGRSRE QRYTKLADWQ 

       490        500        510        520        530        540 
YIEECVQAAS PMPLFGNGDI LSFEDANRAM QTGVTGIMIA RGALLKPWLF TEIKEQRHWD 

       550        560        570        580        590        600 
ISSSERLDIL RDFTNYGLEH WGSDTQGVEK TRRFLLEWLS FLCRYVPVGL LERLPQRINE 

       610        620        630        640        650 
RPPYYLGRDY LETLMASQKA ADWIRISEML LGPVPPSFAF LPKHKANAYK 

« Hide

Isoform 2 [UniParc].

Checksum: D5E6DB1B7E8827E3
Show »

FASTA48252,772
Isoform 3 [UniParc].

Checksum: 96931E7C0C377C89
Show »

FASTA40846,106

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Thyroid.
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT GLY-185.
Tissue: Pancreas, Placenta and Uterus.
[4]The European IMAGE consortium
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-650 (ISOFORM 2).
[5]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-273, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK023958 mRNA. Translation: BAB14740.1.
AC011499 Genomic DNA. No translation available.
BC004549 mRNA. Translation: AAH04549.1.
BC008362 mRNA. Translation: AAH08362.1.
BC009973 mRNA. Translation: AAH09973.1.
AL365411 mRNA. Translation: CAB96955.1.
RefSeqNP_001155091.1. NM_001161619.1.
NP_064560.2. NM_020175.2.
UniGeneHs.284297.

3D structure databases

ProteinModelPortalQ96G46.
SMRQ96G46. Positions 121-182, 306-575.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121258. 19 interactions.
IntActQ96G46. 1 interaction.
STRING9606.ENSP00000311977.

PTM databases

PhosphoSiteQ96G46.

Polymorphism databases

DMDM110808212.

Proteomic databases

PaxDbQ96G46.
PRIDEQ96G46.

Protocols and materials databases

DNASU56931.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000309061; ENSP00000311977; ENSG00000141994. [Q96G46-1]
ENST00000320699; ENSP00000315558; ENSG00000141994. [Q96G46-3]
GeneID56931.
KEGGhsa:56931.
UCSCuc002mdc.3. human. [Q96G46-1]
uc002mdd.3. human. [Q96G46-3]

Organism-specific databases

CTD56931.
GeneCardsGC19M005737.
HGNCHGNC:26920. DUS3L.
HPAHPA041854.
HPA041944.
neXtProtNX_Q96G46.
PharmGKBPA142671938.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0042.
HOGENOMHOG000240610.
HOVERGENHBG062413.
InParanoidQ96G46.
KOK05544.
OMAESAAKCF.
OrthoDBEOG76HQ12.
PhylomeDBQ96G46.
TreeFamTF105726.

Gene expression databases

ArrayExpressQ96G46.
BgeeQ96G46.
CleanExHS_DUS3L.
GenevestigatorQ96G46.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
IPR000571. Znf_CCCH.
[Graphical view]
PANTHERPTHR11082. PTHR11082. 1 hit.
PfamPF01207. Dus. 1 hit.
[Graphical view]
PROSITEPS01136. UPF0034. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi56931.
NextBio62471.
PROQ96G46.

Entry information

Entry nameDUS3L_HUMAN
AccessionPrimary (citable) accession number: Q96G46
Secondary accession number(s): Q96HM5 expand/collapse secondary AC list , Q9BSU4, Q9H877, Q9NPR1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: April 16, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM