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Protein

Protein-lysine N-methyltransferase EEF2KMT

Gene

EEF2KMT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei139 – 1391S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei228 – 2281S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei247 – 2471S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. protein-lysine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. peptidyl-lysine trimethylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-lysine N-methyltransferase EEF2KMTCurated (EC:2.1.1.-1 Publication)
Alternative name(s):
eEF2-lysine methyltransferase
Short name:
eEF2-KMT
Gene namesi
Name:EEF2KMTImported
Synonyms:FAM86AImported
ORF Names:SB153
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:32221. EEF2KMT.

Subcellular locationi

  1. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671858.

Polymorphism and mutation databases

BioMutaiFAM86A.
DMDMi85700958.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Protein-lysine N-methyltransferase EEF2KMTPRO_0000076218Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ96G04.
PRIDEiQ96G04.

PTM databases

PhosphoSiteiQ96G04.

Expressioni

Gene expression databases

BgeeiQ96G04.
CleanExiHS_FAM86A.
ExpressionAtlasiQ96G04. baseline and differential.
GenevestigatoriQ96G04.

Organism-specific databases

HPAiHPA041610.

Interactioni

Subunit structurei

Interacts with FAM86B2 and FAM86C1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PRTFDC1Q9NRG14EBI-747840,EBI-739759

Protein-protein interaction databases

BioGridi128213. 13 interactions.
IntActiQ96G04. 1 interaction.
MINTiMINT-1450986.
STRINGi9606.ENSP00000398502.

Structurei

3D structure databases

ProteinModelPortaliQ96G04.
SMRiQ96G04. Positions 139-313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni165 – 1673S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000047003.
HOGENOMiHOG000067842.
HOVERGENiHBG059594.
InParanoidiQ96G04.
OMAiYPVRRSY.
OrthoDBiEOG74XS7J.
PhylomeDBiQ96G04.
TreeFamiTF326304.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029426. FAM86.
IPR019410. Nicotinamide_N-MeTfrase-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF14904. FAM86. 1 hit.
PF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96G04-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPEENAGTE LLLQSFERRF LAARTLRSFP WQSLEAKLRD SSDSELLRDI
60 70 80 90 100
LHKTVKHPVC VKHPPSVKYA RCFLSELIKK HEAVHTEPLD ELYEALAETL
110 120 130 140 150
MAKESTQGHR SYLLPSGGSV TLSESTAIIS YGTTGLVTWD AALYLAEWAI
160 170 180 190 200
ENPAVFTNRT VLELGSGAGL TGLAICKMCR PRAYIFSDCH SRVLEQLRGN
210 220 230 240 250
VLLNGLSLEA DITAKLDSPR VTVAQLDWDV ATVHQLSAFQ PDVVIAADVL
260 270 280 290 300
YCPEAIMSLV GVLRRLAACR EHQRAPEVYV AFTVRNPETC QLFTTELGRA
310 320 330
GIRWEVEPRH EQKLFPYEEH LEMAMLNLTL
Length:330
Mass (Da):36,915
Last modified:January 24, 2006 - v2
Checksum:i1C1B37B9F6D2EE52
GO
Isoform 2 (identifier: Q96G04-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     81-114: Missing.

Show »
Length:296
Mass (Da):33,050
Checksum:i6198BC10E6254AC9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti123 – 1231S → C.
Corresponds to variant rs9673733 [ dbSNP | Ensembl ].
VAR_033854
Natural varianti230 – 2301V → I.
Corresponds to variant rs13099404 [ dbSNP | Ensembl ].
VAR_067704
Natural varianti270 – 2701R → W.
Corresponds to variant rs3204207 [ dbSNP | Ensembl ].
VAR_060160
Natural varianti272 – 2721H → D.2 Publications
Corresponds to variant rs12928528 [ dbSNP | Ensembl ].
VAR_067705
Natural varianti329 – 3291T → I.
Corresponds to variant rs1047478 [ dbSNP | Ensembl ].
VAR_067706

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei81 – 11434Missing in isoform 2. 1 PublicationVSP_017097Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037162 mRNA. Translation: AAK67640.1.
CH471112 Genomic DNA. Translation: EAW85235.1.
CH471112 Genomic DNA. Translation: EAW85236.1.
BC010084 mRNA. Translation: AAH10084.1.
CCDSiCCDS10529.1. [Q96G04-1]
CCDS10530.1. [Q96G04-2]
RefSeqiNP_001275958.1. NM_001289029.1.
NP_958802.1. NM_201400.3. [Q96G04-1]
NP_963892.1. NM_201598.3. [Q96G04-2]
UniGeneiHs.406461.
Hs.592086.

Genome annotation databases

EnsembliENST00000427587; ENSP00000398502; ENSG00000118894. [Q96G04-1]
ENST00000458008; ENSP00000389710; ENSG00000118894. [Q96G04-2]
GeneIDi196483.
KEGGihsa:196483.
UCSCiuc002cyo.2. human. [Q96G04-1]
uc002cyp.2. human. [Q96G04-2]

Polymorphism and mutation databases

BioMutaiFAM86A.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037162 mRNA. Translation: AAK67640.1.
CH471112 Genomic DNA. Translation: EAW85235.1.
CH471112 Genomic DNA. Translation: EAW85236.1.
BC010084 mRNA. Translation: AAH10084.1.
CCDSiCCDS10529.1. [Q96G04-1]
CCDS10530.1. [Q96G04-2]
RefSeqiNP_001275958.1. NM_001289029.1.
NP_958802.1. NM_201400.3. [Q96G04-1]
NP_963892.1. NM_201598.3. [Q96G04-2]
UniGeneiHs.406461.
Hs.592086.

3D structure databases

ProteinModelPortaliQ96G04.
SMRiQ96G04. Positions 139-313.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128213. 13 interactions.
IntActiQ96G04. 1 interaction.
MINTiMINT-1450986.
STRINGi9606.ENSP00000398502.

PTM databases

PhosphoSiteiQ96G04.

Polymorphism and mutation databases

BioMutaiFAM86A.
DMDMi85700958.

Proteomic databases

MaxQBiQ96G04.
PRIDEiQ96G04.

Protocols and materials databases

DNASUi196483.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000427587; ENSP00000398502; ENSG00000118894. [Q96G04-1]
ENST00000458008; ENSP00000389710; ENSG00000118894. [Q96G04-2]
GeneIDi196483.
KEGGihsa:196483.
UCSCiuc002cyo.2. human. [Q96G04-1]
uc002cyp.2. human. [Q96G04-2]

Organism-specific databases

CTDi196483.
GeneCardsiGC16M005134.
HGNCiHGNC:32221. EEF2KMT.
HPAiHPA041610.
MIMi615263. gene.
neXtProtiNX_Q96G04.
PharmGKBiPA142671858.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00510000047003.
HOGENOMiHOG000067842.
HOVERGENiHBG059594.
InParanoidiQ96G04.
OMAiYPVRRSY.
OrthoDBiEOG74XS7J.
PhylomeDBiQ96G04.
TreeFamiTF326304.

Miscellaneous databases

GenomeRNAii196483.
NextBioi89501.
PROiQ96G04.
SOURCEiSearch...

Gene expression databases

BgeeiQ96G04.
CleanExiHS_FAM86A.
ExpressionAtlasiQ96G04. baseline and differential.
GenevestigatoriQ96G04.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029426. FAM86.
IPR019410. Nicotinamide_N-MeTfrase-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF14904. FAM86. 1 hit.
PF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Li N., Zhang M., Wan T., Zhang W., Cao X.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASP-272.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-272.
    Tissue: Lung.
  4. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity."
    Cloutier P., Lavallee-Adam M., Faubert D., Blanchette M., Coulombe B.
    PLoS Genet. 9:E1003210-E1003210(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FAM86B2 AND FAM86C1, SUBCELLULAR LOCATION.
  6. "Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2)."
    Davydova E., Ho A.Y., Malecki J., Moen A., Enserink J.M., Jakobsson M.E., Loenarz C., Falnes P.O.
    J. Biol. Chem. 289:30499-30510(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiEF2KT_HUMAN
AccessioniPrimary (citable) accession number: Q96G04
Secondary accession number(s): D3DUF0, Q96S85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: April 29, 2015
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.