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Protein

Protein-lysine N-methyltransferase EEF2KMT

Gene

EEF2KMT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei139S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei228S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei247S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • protein-lysine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • peptidyl-lysine trimethylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciZFISH:ENSG00000118894-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-lysine N-methyltransferase EEF2KMTCurated (EC:2.1.1.-1 Publication)
Alternative name(s):
eEF2-lysine methyltransferase
Short name:
eEF2-KMT
Gene namesi
Name:EEF2KMTImported
Synonyms:FAM86AImported
ORF Names:SB153
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:32221. EEF2KMT.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000118894.
PharmGKBiPA142671858.

Polymorphism and mutation databases

BioMutaiFAM86A.
DMDMi85700958.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762181 – 330Protein-lysine N-methyltransferase EEF2KMTAdd BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ96G04.
PaxDbiQ96G04.
PeptideAtlasiQ96G04.
PRIDEiQ96G04.

PTM databases

iPTMnetiQ96G04.
PhosphoSitePlusiQ96G04.

Expressioni

Gene expression databases

BgeeiENSG00000118894.
CleanExiHS_FAM86A.
ExpressionAtlasiQ96G04. baseline and differential.
GenevisibleiQ96G04. HS.

Organism-specific databases

HPAiHPA041610.

Interactioni

Subunit structurei

Interacts with FAM86B2 and FAM86C1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PRTFDC1Q9NRG16EBI-747840,EBI-739759

Protein-protein interaction databases

BioGridi128213. 13 interactors.
IntActiQ96G04. 7 interactors.
MINTiMINT-1450986.
STRINGi9606.ENSP00000398502.

Structurei

3D structure databases

ProteinModelPortaliQ96G04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni165 – 167S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2497. Eukaryota.
ENOG410ZQK4. LUCA.
GeneTreeiENSGT00510000047003.
HOGENOMiHOG000067842.
HOVERGENiHBG059594.
InParanoidiQ96G04.
OMAiPVKKMAW.
OrthoDBiEOG091G0E1D.
PhylomeDBiQ96G04.
TreeFamiTF326304.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029426. FAM86.
IPR019410. Methyltransf_16.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF14904. FAM86. 1 hit.
PF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96G04-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPEENAGTE LLLQSFERRF LAARTLRSFP WQSLEAKLRD SSDSELLRDI
60 70 80 90 100
LHKTVKHPVC VKHPPSVKYA RCFLSELIKK HEAVHTEPLD ELYEALAETL
110 120 130 140 150
MAKESTQGHR SYLLPSGGSV TLSESTAIIS YGTTGLVTWD AALYLAEWAI
160 170 180 190 200
ENPAVFTNRT VLELGSGAGL TGLAICKMCR PRAYIFSDCH SRVLEQLRGN
210 220 230 240 250
VLLNGLSLEA DITAKLDSPR VTVAQLDWDV ATVHQLSAFQ PDVVIAADVL
260 270 280 290 300
YCPEAIMSLV GVLRRLAACR EHQRAPEVYV AFTVRNPETC QLFTTELGRA
310 320 330
GIRWEVEPRH EQKLFPYEEH LEMAMLNLTL
Length:330
Mass (Da):36,915
Last modified:January 24, 2006 - v2
Checksum:i1C1B37B9F6D2EE52
GO
Isoform 2 (identifier: Q96G04-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     81-114: Missing.

Show »
Length:296
Mass (Da):33,050
Checksum:i6198BC10E6254AC9
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_033854123S → C.Corresponds to variant rs9673733dbSNPEnsembl.1
Natural variantiVAR_067704230V → I.Corresponds to variant rs148557961dbSNPEnsembl.1
Natural variantiVAR_060160270R → W.Corresponds to variant rs3204207dbSNPEnsembl.1
Natural variantiVAR_067705272H → D.2 PublicationsCorresponds to variant rs12928528dbSNPEnsembl.1
Natural variantiVAR_067706329T → I.Corresponds to variant rs760584474dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01709781 – 114Missing in isoform 2. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037162 mRNA. Translation: AAK67640.1.
CH471112 Genomic DNA. Translation: EAW85235.1.
CH471112 Genomic DNA. Translation: EAW85236.1.
BC010084 mRNA. Translation: AAH10084.1.
CCDSiCCDS10529.1. [Q96G04-1]
CCDS10530.1. [Q96G04-2]
RefSeqiNP_001275958.1. NM_001289029.1.
NP_958802.1. NM_201400.3. [Q96G04-1]
NP_963892.1. NM_201598.3. [Q96G04-2]
UniGeneiHs.406461.
Hs.592086.

Genome annotation databases

EnsembliENST00000427587; ENSP00000398502; ENSG00000118894. [Q96G04-1]
ENST00000458008; ENSP00000389710; ENSG00000118894. [Q96G04-2]
GeneIDi196483.
KEGGihsa:196483.
UCSCiuc002cyo.4. human. [Q96G04-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037162 mRNA. Translation: AAK67640.1.
CH471112 Genomic DNA. Translation: EAW85235.1.
CH471112 Genomic DNA. Translation: EAW85236.1.
BC010084 mRNA. Translation: AAH10084.1.
CCDSiCCDS10529.1. [Q96G04-1]
CCDS10530.1. [Q96G04-2]
RefSeqiNP_001275958.1. NM_001289029.1.
NP_958802.1. NM_201400.3. [Q96G04-1]
NP_963892.1. NM_201598.3. [Q96G04-2]
UniGeneiHs.406461.
Hs.592086.

3D structure databases

ProteinModelPortaliQ96G04.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128213. 13 interactors.
IntActiQ96G04. 7 interactors.
MINTiMINT-1450986.
STRINGi9606.ENSP00000398502.

PTM databases

iPTMnetiQ96G04.
PhosphoSitePlusiQ96G04.

Polymorphism and mutation databases

BioMutaiFAM86A.
DMDMi85700958.

Proteomic databases

MaxQBiQ96G04.
PaxDbiQ96G04.
PeptideAtlasiQ96G04.
PRIDEiQ96G04.

Protocols and materials databases

DNASUi196483.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000427587; ENSP00000398502; ENSG00000118894. [Q96G04-1]
ENST00000458008; ENSP00000389710; ENSG00000118894. [Q96G04-2]
GeneIDi196483.
KEGGihsa:196483.
UCSCiuc002cyo.4. human. [Q96G04-1]

Organism-specific databases

CTDi196483.
GeneCardsiEEF2KMT.
HGNCiHGNC:32221. EEF2KMT.
HPAiHPA041610.
MIMi615263. gene.
neXtProtiNX_Q96G04.
OpenTargetsiENSG00000118894.
PharmGKBiPA142671858.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2497. Eukaryota.
ENOG410ZQK4. LUCA.
GeneTreeiENSGT00510000047003.
HOGENOMiHOG000067842.
HOVERGENiHBG059594.
InParanoidiQ96G04.
OMAiPVKKMAW.
OrthoDBiEOG091G0E1D.
PhylomeDBiQ96G04.
TreeFamiTF326304.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000118894-MONOMER.

Miscellaneous databases

GenomeRNAii196483.
PROiQ96G04.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000118894.
CleanExiHS_FAM86A.
ExpressionAtlasiQ96G04. baseline and differential.
GenevisibleiQ96G04. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029426. FAM86.
IPR019410. Methyltransf_16.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF14904. FAM86. 1 hit.
PF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEF2KT_HUMAN
AccessioniPrimary (citable) accession number: Q96G04
Secondary accession number(s): D3DUF0, Q96S85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: November 30, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.