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Q96FZ7

- CHMP6_HUMAN

UniProt

Q96FZ7 - CHMP6_HUMAN

Protein

Charged multivesicular body protein 6

Gene

CHMP6

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein N-terminus binding Source: UniProtKB

    GO - Biological processi

    1. endosomal transport Source: Reactome
    2. membrane organization Source: Reactome
    3. protein transport Source: UniProtKB-KW
    4. viral life cycle Source: Reactome
    5. viral process Source: Reactome

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).
    REACT_6359. Budding and maturation of HIV virion.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Charged multivesicular body protein 6
    Alternative name(s):
    Chromatin-modifying protein 6
    Vacuolar protein sorting-associated protein 20
    Short name:
    Vps20
    Short name:
    hVps20
    Gene namesi
    Name:CHMP6
    Synonyms:VPS20
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:25675. CHMP6.

    Subcellular locationi

    Endomembrane system. Endosome membrane; Lipid-anchor. Late endosome membrane Curated
    Note: Localizes to endosomal membranes.

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. extracellular vesicular exosome Source: UniProt
    3. late endosome membrane Source: UniProtKB-SubCell
    4. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi2 – 21G → A: Abolishes myristoylation. 2 Publications
    Mutagenesisi49 – 491R → E: Does not affect the subcellular location. 2 Publications
    Mutagenesisi168 – 20134Missing: Membrane association; releases autoinhibition. 1 PublicationAdd
    BLAST
    Mutagenesisi170 – 1701L → D: Abolishes interaction with VPS4A. 2 Publications
    Mutagenesisi173 – 1731V → D: Abolishes interaction with VPS4A. 2 Publications
    Mutagenesisi178 – 1781L → D: Reduces interaction with VPS4A. 2 Publications

    Organism-specific databases

    PharmGKBiPA142672114.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedCurated
    Chaini2 – 201200Charged multivesicular body protein 6PRO_0000211508Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine1 Publication

    Post-translational modificationi

    ISGylated in a CHMP5-dependent manner. Isgylation weakens its interaction with VPS4A.1 Publication

    Keywords - PTMi

    Lipoprotein, Myristate, Ubl conjugation

    Proteomic databases

    MaxQBiQ96FZ7.
    PaxDbiQ96FZ7.
    PRIDEiQ96FZ7.

    PTM databases

    PhosphoSiteiQ96FZ7.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ96FZ7.
    BgeeiQ96FZ7.
    CleanExiHS_CHMP6.
    GenevestigatoriQ96FZ7.

    Organism-specific databases

    HPAiHPA023001.
    HPA024460.

    Interactioni

    Subunit structurei

    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Interacts with VPS4A; the interaction is direct. Interacts with VPS4B; the interaction is direct. Interacts with CHMP4A, CHMP4B and CHMP4C. Interacts with SNF8, VPS25 and VPS36.6 Publications

    Protein-protein interaction databases

    BioGridi122771. 9 interactions.
    IntActiQ96FZ7. 3 interactions.
    MINTiMINT-5003442.
    STRINGi9606.ENSP00000317468.

    Structurei

    Secondary structure

    1
    201
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi15 – 4329

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2K3WNMR-B166-181[»]
    3HTUX-ray2.00B/D/F/H11-48[»]
    ProteinModelPortaliQ96FZ7.
    SMRiQ96FZ7. Positions 17-92, 118-170.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ96FZ7.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni170 – 18112Interaction with VPS4AAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili10 – 145136Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi168 – 17912Type-2 MIT-interacting motifAdd
    BLAST

    Domaini

    The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.

    Sequence similaritiesi

    Belongs to the SNF7 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG291677.
    HOGENOMiHOG000208642.
    HOVERGENiHBG080510.
    InParanoidiQ96FZ7.
    KOiK12195.
    OMAiECLARDD.
    PhylomeDBiQ96FZ7.
    TreeFamiTF105929.

    Family and domain databases

    InterProiIPR005024. Snf7.
    [Graphical view]
    PfamiPF03357. Snf7. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q96FZ7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNLFGRKKQ SRVTEQDKAI LQLKQQRDKL RQYQKRIAQQ LERERALARQ    50
    LLRDGRKERA KLLLKKKRYQ EQLLDRTENQ ISSLEAMVQS IEFTQIEMKV 100
    MEGLQFGNEC LNKMHQVMSI EEVERILDET QEAVEYQRQI DELLAGSFTQ 150
    EDEDAILEEL SAITQEQIEL PEVPSEPLPE KIPENVPVKA RPRQAELVAA 200
    S 201
    Length:201
    Mass (Da):23,485
    Last modified:January 23, 2007 - v3
    Checksum:i0D490C4DE047DC02
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171D → G in BAD96907. 1 PublicationCurated
    Sequence conflicti106 – 1061F → L in BAB13901. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti55 – 551G → S.
    Corresponds to variant rs61037507 [ dbSNP | Ensembl ].
    VAR_061807

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY329087 mRNA. Translation: AAQ91196.1.
    AK021811 mRNA. Translation: BAB13901.1.
    CR457284 mRNA. Translation: CAG33565.1.
    AK223187 mRNA. Translation: BAD96907.1.
    AK292105 mRNA. Translation: BAF84794.1.
    BC010108 mRNA. Translation: AAH10108.1.
    CCDSiCCDS11774.1.
    RefSeqiNP_078867.2. NM_024591.4.
    UniGeneiHs.514560.

    Genome annotation databases

    EnsembliENST00000325167; ENSP00000317468; ENSG00000176108.
    GeneIDi79643.
    KEGGihsa:79643.
    UCSCiuc002jyw.4. human.

    Polymorphism databases

    DMDMi73917777.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY329087 mRNA. Translation: AAQ91196.1 .
    AK021811 mRNA. Translation: BAB13901.1 .
    CR457284 mRNA. Translation: CAG33565.1 .
    AK223187 mRNA. Translation: BAD96907.1 .
    AK292105 mRNA. Translation: BAF84794.1 .
    BC010108 mRNA. Translation: AAH10108.1 .
    CCDSi CCDS11774.1.
    RefSeqi NP_078867.2. NM_024591.4.
    UniGenei Hs.514560.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2K3W NMR - B 166-181 [» ]
    3HTU X-ray 2.00 B/D/F/H 11-48 [» ]
    ProteinModelPortali Q96FZ7.
    SMRi Q96FZ7. Positions 17-92, 118-170.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122771. 9 interactions.
    IntActi Q96FZ7. 3 interactions.
    MINTi MINT-5003442.
    STRINGi 9606.ENSP00000317468.

    PTM databases

    PhosphoSitei Q96FZ7.

    Polymorphism databases

    DMDMi 73917777.

    Proteomic databases

    MaxQBi Q96FZ7.
    PaxDbi Q96FZ7.
    PRIDEi Q96FZ7.

    Protocols and materials databases

    DNASUi 79643.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000325167 ; ENSP00000317468 ; ENSG00000176108 .
    GeneIDi 79643.
    KEGGi hsa:79643.
    UCSCi uc002jyw.4. human.

    Organism-specific databases

    CTDi 79643.
    GeneCardsi GC17P078965.
    HGNCi HGNC:25675. CHMP6.
    HPAi HPA023001.
    HPA024460.
    MIMi 610901. gene.
    neXtProti NX_Q96FZ7.
    PharmGKBi PA142672114.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG291677.
    HOGENOMi HOG000208642.
    HOVERGENi HBG080510.
    InParanoidi Q96FZ7.
    KOi K12195.
    OMAi ECLARDD.
    PhylomeDBi Q96FZ7.
    TreeFami TF105929.

    Enzyme and pathway databases

    Reactomei REACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).
    REACT_6359. Budding and maturation of HIV virion.

    Miscellaneous databases

    EvolutionaryTracei Q96FZ7.
    GeneWikii CHMP6.
    GenomeRNAii 79643.
    NextBioi 68773.
    PROi Q96FZ7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96FZ7.
    Bgeei Q96FZ7.
    CleanExi HS_CHMP6.
    Genevestigatori Q96FZ7.

    Family and domain databases

    InterProi IPR005024. Snf7.
    [Graphical view ]
    Pfami PF03357. Snf7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and function of human Vps20 and Snf7 proteins."
      Peck J.W., Bowden E.T., Burbelo P.D.
      Biochem. J. 377:693-700(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo and Synovial cell.
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney proximal tubule.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Uterus.
    6. Cited for: INTERACTION WITH VPS25; CHMP4B; VPS4A AND VPS4B.
    7. "Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins."
      Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D.
      Proc. Natl. Acad. Sci. U.S.A. 100:12414-12419(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHMP4A; CHMP4B; CHMP4C; VPS25; SNF8 AND VPS36.
    8. Erratum
      Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D.
      Proc. Natl. Acad. Sci. U.S.A. 100:152845-152845(2003)
    9. "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting."
      Yorikawa C., Shibata H., Waguri S., Hatta K., Horii M., Katoh K., Kobayashi T., Uchiyama Y., Maki M.
      Biochem. J. 387:17-26(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MYRISTOYLATION AT GLY-2, INTERACTION WITH CHMP4B AND VPS25, MUTAGENESIS OF GLY-2 AND ARG-49.
    10. "Structure/function analysis of four core ESCRT-III proteins reveals common regulatory role for extreme C-terminal domain."
      Shim S., Kimpler L.A., Hanson P.I.
      Traffic 8:1068-1079(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: AUTOINHIBITORY MECHANISM, INTERACTION, MUTAGENESIS OF 168-ILE--SER-201.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15."
      Kuang Z., Seo E.J., Leis J.
      J. Virol. 85:7153-7161(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISGYLATION, INTERACTION WITH VPS4A.
    13. "Two distinct modes of ESCRT-III recognition are required for VPS4 functions in lysosomal protein targeting and HIV-1 budding."
      Kieffer C., Skalicky J.J., Morita E., De Domenico I., Ward D.M., Kaplan J., Sundquist W.I.
      Dev. Cell 15:62-73(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 168-179 IN COMPLEX WITH VPS4A, MUTAGENESIS OF LEU-170; VAL-173 AND LEU-178.

    Entry informationi

    Entry nameiCHMP6_HUMAN
    AccessioniPrimary (citable) accession number: Q96FZ7
    Secondary accession number(s): A8K7U0, Q53FU4, Q9HAE8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 112 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3