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Q96FZ7

- CHMP6_HUMAN

UniProt

Q96FZ7 - CHMP6_HUMAN

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Protein

Charged multivesicular body protein 6

Gene

CHMP6

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.

GO - Molecular functioni

  1. protein N-terminus binding Source: UniProtKB

GO - Biological processi

  1. endosomal transport Source: Reactome
  2. membrane organization Source: Reactome
  3. protein transport Source: UniProtKB-KW
  4. viral life cycle Source: Reactome
  5. viral process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).
REACT_6359. Budding and maturation of HIV virion.

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 6
Alternative name(s):
Chromatin-modifying protein 6
Vacuolar protein sorting-associated protein 20
Short name:
Vps20
Short name:
hVps20
Gene namesi
Name:CHMP6
Synonyms:VPS20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:25675. CHMP6.

Subcellular locationi

Endomembrane system. Endosome membrane; Lipid-anchor. Late endosome membrane Curated
Note: Localizes to endosomal membranes.

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. endosome Source: UniProtKB-KW
  3. extracellular vesicular exosome Source: UniProt
  4. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21G → A: Abolishes myristoylation. 1 Publication
Mutagenesisi49 – 491R → E: Does not affect the subcellular location. 1 Publication
Mutagenesisi168 – 20134Missing: Membrane association; releases autoinhibition. 1 PublicationAdd
BLAST
Mutagenesisi170 – 1701L → D: Abolishes interaction with VPS4A. 1 Publication
Mutagenesisi173 – 1731V → D: Abolishes interaction with VPS4A. 1 Publication
Mutagenesisi178 – 1781L → D: Reduces interaction with VPS4A. 1 Publication

Organism-specific databases

PharmGKBiPA142672114.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedCurated
Chaini2 – 201200Charged multivesicular body protein 6PRO_0000211508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication

Post-translational modificationi

ISGylated in a CHMP5-dependent manner. Isgylation weakens its interaction with VPS4A.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Ubl conjugation

Proteomic databases

MaxQBiQ96FZ7.
PaxDbiQ96FZ7.
PRIDEiQ96FZ7.

PTM databases

PhosphoSiteiQ96FZ7.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiQ96FZ7.
CleanExiHS_CHMP6.
ExpressionAtlasiQ96FZ7. baseline and differential.
GenevestigatoriQ96FZ7.

Organism-specific databases

HPAiHPA023001.
HPA024460.

Interactioni

Subunit structurei

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Interacts with VPS4A; the interaction is direct. Interacts with VPS4B; the interaction is direct. Interacts with CHMP4A, CHMP4B and CHMP4C. Interacts with SNF8, VPS25 and VPS36.6 Publications

Protein-protein interaction databases

BioGridi122771. 9 interactions.
IntActiQ96FZ7. 3 interactions.
MINTiMINT-5003442.
STRINGi9606.ENSP00000317468.

Structurei

Secondary structure

1
201
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 4329Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K3WNMR-B166-181[»]
3HTUX-ray2.00B/D/F/H11-48[»]
ProteinModelPortaliQ96FZ7.
SMRiQ96FZ7. Positions 17-92, 118-170.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96FZ7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni170 – 18112Interaction with VPS4AAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili10 – 145136Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi168 – 17912Type-2 MIT-interacting motifAdd
BLAST

Domaini

The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG291677.
GeneTreeiENSGT00720000108863.
HOGENOMiHOG000208642.
HOVERGENiHBG080510.
InParanoidiQ96FZ7.
KOiK12195.
OMAiECLARDD.
PhylomeDBiQ96FZ7.
TreeFamiTF105929.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96FZ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGNLFGRKKQ SRVTEQDKAI LQLKQQRDKL RQYQKRIAQQ LERERALARQ
60 70 80 90 100
LLRDGRKERA KLLLKKKRYQ EQLLDRTENQ ISSLEAMVQS IEFTQIEMKV
110 120 130 140 150
MEGLQFGNEC LNKMHQVMSI EEVERILDET QEAVEYQRQI DELLAGSFTQ
160 170 180 190 200
EDEDAILEEL SAITQEQIEL PEVPSEPLPE KIPENVPVKA RPRQAELVAA

S
Length:201
Mass (Da):23,485
Last modified:January 23, 2007 - v3
Checksum:i0D490C4DE047DC02
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171D → G in BAD96907. 1 PublicationCurated
Sequence conflicti106 – 1061F → L in BAB13901. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti55 – 551G → S.
Corresponds to variant rs61037507 [ dbSNP | Ensembl ].
VAR_061807

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY329087 mRNA. Translation: AAQ91196.1.
AK021811 mRNA. Translation: BAB13901.1.
CR457284 mRNA. Translation: CAG33565.1.
AK223187 mRNA. Translation: BAD96907.1.
AK292105 mRNA. Translation: BAF84794.1.
BC010108 mRNA. Translation: AAH10108.1.
CCDSiCCDS11774.1.
RefSeqiNP_078867.2. NM_024591.4.
UniGeneiHs.514560.

Genome annotation databases

EnsembliENST00000325167; ENSP00000317468; ENSG00000176108.
GeneIDi79643.
KEGGihsa:79643.
UCSCiuc002jyw.4. human.

Polymorphism databases

DMDMi73917777.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY329087 mRNA. Translation: AAQ91196.1 .
AK021811 mRNA. Translation: BAB13901.1 .
CR457284 mRNA. Translation: CAG33565.1 .
AK223187 mRNA. Translation: BAD96907.1 .
AK292105 mRNA. Translation: BAF84794.1 .
BC010108 mRNA. Translation: AAH10108.1 .
CCDSi CCDS11774.1.
RefSeqi NP_078867.2. NM_024591.4.
UniGenei Hs.514560.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2K3W NMR - B 166-181 [» ]
3HTU X-ray 2.00 B/D/F/H 11-48 [» ]
ProteinModelPortali Q96FZ7.
SMRi Q96FZ7. Positions 17-92, 118-170.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122771. 9 interactions.
IntActi Q96FZ7. 3 interactions.
MINTi MINT-5003442.
STRINGi 9606.ENSP00000317468.

PTM databases

PhosphoSitei Q96FZ7.

Polymorphism databases

DMDMi 73917777.

Proteomic databases

MaxQBi Q96FZ7.
PaxDbi Q96FZ7.
PRIDEi Q96FZ7.

Protocols and materials databases

DNASUi 79643.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000325167 ; ENSP00000317468 ; ENSG00000176108 .
GeneIDi 79643.
KEGGi hsa:79643.
UCSCi uc002jyw.4. human.

Organism-specific databases

CTDi 79643.
GeneCardsi GC17P078965.
HGNCi HGNC:25675. CHMP6.
HPAi HPA023001.
HPA024460.
MIMi 610901. gene.
neXtProti NX_Q96FZ7.
PharmGKBi PA142672114.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG291677.
GeneTreei ENSGT00720000108863.
HOGENOMi HOG000208642.
HOVERGENi HBG080510.
InParanoidi Q96FZ7.
KOi K12195.
OMAi ECLARDD.
PhylomeDBi Q96FZ7.
TreeFami TF105929.

Enzyme and pathway databases

Reactomei REACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).
REACT_6359. Budding and maturation of HIV virion.

Miscellaneous databases

EvolutionaryTracei Q96FZ7.
GeneWikii CHMP6.
GenomeRNAii 79643.
NextBioi 68773.
PROi Q96FZ7.
SOURCEi Search...

Gene expression databases

Bgeei Q96FZ7.
CleanExi HS_CHMP6.
ExpressionAtlasi Q96FZ7. baseline and differential.
Genevestigatori Q96FZ7.

Family and domain databases

InterProi IPR005024. Snf7_fam.
[Graphical view ]
Pfami PF03357. Snf7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and function of human Vps20 and Snf7 proteins."
    Peck J.W., Bowden E.T., Burbelo P.D.
    Biochem. J. 377:693-700(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo and Synovial cell.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney proximal tubule.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Uterus.
  6. Cited for: INTERACTION WITH VPS25; CHMP4B; VPS4A AND VPS4B.
  7. "Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins."
    Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D.
    Proc. Natl. Acad. Sci. U.S.A. 100:12414-12419(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHMP4A; CHMP4B; CHMP4C; VPS25; SNF8 AND VPS36.
  8. Erratum
    Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D.
    Proc. Natl. Acad. Sci. U.S.A. 100:152845-152845(2003)
  9. "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting."
    Yorikawa C., Shibata H., Waguri S., Hatta K., Horii M., Katoh K., Kobayashi T., Uchiyama Y., Maki M.
    Biochem. J. 387:17-26(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MYRISTOYLATION AT GLY-2, INTERACTION WITH CHMP4B AND VPS25, MUTAGENESIS OF GLY-2 AND ARG-49.
  10. "Structure/function analysis of four core ESCRT-III proteins reveals common regulatory role for extreme C-terminal domain."
    Shim S., Kimpler L.A., Hanson P.I.
    Traffic 8:1068-1079(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: AUTOINHIBITORY MECHANISM, INTERACTION, MUTAGENESIS OF 168-ILE--SER-201.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15."
    Kuang Z., Seo E.J., Leis J.
    J. Virol. 85:7153-7161(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISGYLATION, INTERACTION WITH VPS4A.
  13. "Two distinct modes of ESCRT-III recognition are required for VPS4 functions in lysosomal protein targeting and HIV-1 budding."
    Kieffer C., Skalicky J.J., Morita E., De Domenico I., Ward D.M., Kaplan J., Sundquist W.I.
    Dev. Cell 15:62-73(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 168-179 IN COMPLEX WITH VPS4A, MUTAGENESIS OF LEU-170; VAL-173 AND LEU-178.

Entry informationi

Entry nameiCHMP6_HUMAN
AccessioniPrimary (citable) accession number: Q96FZ7
Secondary accession number(s): A8K7U0, Q53FU4, Q9HAE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3