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Protein

Charged multivesicular body protein 6

Gene

CHMP6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB

GO - Biological processi

  • autophagy Source: ParkinsonsUK-UCL
  • cell separation after cytokinesis Source: UniProtKB
  • endosomal transport Source: Reactome
  • ESCRT III complex assembly Source: ParkinsonsUK-UCL
  • mitotic metaphase plate congression Source: UniProtKB
  • multivesicular body assembly Source: ParkinsonsUK-UCL
  • nucleus organization Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • viral budding via host ESCRT complex Source: UniProtKB
  • viral life cycle Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-162588. Budding and maturation of HIV virion.
R-HSA-1632852. Macroautophagy.
R-HSA-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 6
Alternative name(s):
Chromatin-modifying protein 6
Vacuolar protein sorting-associated protein 20
Short name:
Vps20
Short name:
hVps20
Gene namesi
Name:CHMP6
Synonyms:VPS20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:25675. CHMP6.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • endosome membrane Source: UniProtKB
  • ESCRT III complex Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • late endosome membrane Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Abolishes myristoylation. 1 Publication1
Mutagenesisi49R → E: Does not affect the subcellular location. 1 Publication1
Mutagenesisi168 – 201Missing : Membrane association; releases autoinhibition. 1 PublicationAdd BLAST34
Mutagenesisi170L → D: Abolishes interaction with VPS4A. 1 Publication1
Mutagenesisi173V → D: Abolishes interaction with VPS4A. 1 Publication1
Mutagenesisi178L → D: Reduces interaction with VPS4A. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000176108.
PharmGKBiPA142672114.

Polymorphism and mutation databases

BioMutaiCHMP6.
DMDMi73917777.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002115082 – 201Charged multivesicular body protein 6Add BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1 Publication1
Modified residuei119PhosphoserineBy similarity1
Modified residuei130PhosphothreonineCombined sources1

Post-translational modificationi

ISGylated in a CHMP5-dependent manner. Isgylation weakens its interaction with VPS4A.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96FZ7.
MaxQBiQ96FZ7.
PaxDbiQ96FZ7.
PeptideAtlasiQ96FZ7.
PRIDEiQ96FZ7.

PTM databases

iPTMnetiQ96FZ7.
PhosphoSitePlusiQ96FZ7.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000176108.
CleanExiHS_CHMP6.
ExpressionAtlasiQ96FZ7. baseline and differential.
GenevisibleiQ96FZ7. HS.

Organism-specific databases

HPAiHPA023001.
HPA024460.

Interactioni

Subunit structurei

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Interacts with VPS4A; the interaction is direct. Interacts with VPS4B; the interaction is direct. Interacts with CHMP4A, CHMP4B and CHMP4C. Interacts with SNF8, VPS25 and VPS36.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS25Q9BRG14EBI-1049648,EBI-741945

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi122771. 12 interactors.
IntActiQ96FZ7. 12 interactors.
MINTiMINT-5003442.
STRINGi9606.ENSP00000317468.

Structurei

Secondary structure

1201
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 43Combined sources29

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K3WNMR-B166-181[»]
3HTUX-ray2.00B/D/F/H11-48[»]
ProteinModelPortaliQ96FZ7.
SMRiQ96FZ7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96FZ7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni170 – 181Interaction with VPS4AAdd BLAST12

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili10 – 145Sequence analysisAdd BLAST136

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi168 – 179Type-2 MIT-interacting motifAdd BLAST12

Domaini

The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2910. Eukaryota.
ENOG4111HN3. LUCA.
GeneTreeiENSGT00720000108863.
HOGENOMiHOG000208642.
HOVERGENiHBG080510.
InParanoidiQ96FZ7.
KOiK12195.
OMAiRKCLQQG.
PhylomeDBiQ96FZ7.
TreeFamiTF105929.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96FZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNLFGRKKQ SRVTEQDKAI LQLKQQRDKL RQYQKRIAQQ LERERALARQ
60 70 80 90 100
LLRDGRKERA KLLLKKKRYQ EQLLDRTENQ ISSLEAMVQS IEFTQIEMKV
110 120 130 140 150
MEGLQFGNEC LNKMHQVMSI EEVERILDET QEAVEYQRQI DELLAGSFTQ
160 170 180 190 200
EDEDAILEEL SAITQEQIEL PEVPSEPLPE KIPENVPVKA RPRQAELVAA

S
Length:201
Mass (Da):23,485
Last modified:January 23, 2007 - v3
Checksum:i0D490C4DE047DC02
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17D → G in BAD96907 (Ref. 4) Curated1
Sequence conflicti106F → L in BAB13901 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06180755G → S.Corresponds to variant rs61037507dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY329087 mRNA. Translation: AAQ91196.1.
AK021811 mRNA. Translation: BAB13901.1.
CR457284 mRNA. Translation: CAG33565.1.
AK223187 mRNA. Translation: BAD96907.1.
AK292105 mRNA. Translation: BAF84794.1.
BC010108 mRNA. Translation: AAH10108.1.
CCDSiCCDS11774.1.
RefSeqiNP_078867.2. NM_024591.4.
UniGeneiHs.514560.

Genome annotation databases

EnsembliENST00000325167; ENSP00000317468; ENSG00000176108.
GeneIDi79643.
KEGGihsa:79643.
UCSCiuc002jyw.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY329087 mRNA. Translation: AAQ91196.1.
AK021811 mRNA. Translation: BAB13901.1.
CR457284 mRNA. Translation: CAG33565.1.
AK223187 mRNA. Translation: BAD96907.1.
AK292105 mRNA. Translation: BAF84794.1.
BC010108 mRNA. Translation: AAH10108.1.
CCDSiCCDS11774.1.
RefSeqiNP_078867.2. NM_024591.4.
UniGeneiHs.514560.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K3WNMR-B166-181[»]
3HTUX-ray2.00B/D/F/H11-48[»]
ProteinModelPortaliQ96FZ7.
SMRiQ96FZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122771. 12 interactors.
IntActiQ96FZ7. 12 interactors.
MINTiMINT-5003442.
STRINGi9606.ENSP00000317468.

PTM databases

iPTMnetiQ96FZ7.
PhosphoSitePlusiQ96FZ7.

Polymorphism and mutation databases

BioMutaiCHMP6.
DMDMi73917777.

Proteomic databases

EPDiQ96FZ7.
MaxQBiQ96FZ7.
PaxDbiQ96FZ7.
PeptideAtlasiQ96FZ7.
PRIDEiQ96FZ7.

Protocols and materials databases

DNASUi79643.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325167; ENSP00000317468; ENSG00000176108.
GeneIDi79643.
KEGGihsa:79643.
UCSCiuc002jyw.4. human.

Organism-specific databases

CTDi79643.
GeneCardsiCHMP6.
HGNCiHGNC:25675. CHMP6.
HPAiHPA023001.
HPA024460.
MIMi610901. gene.
neXtProtiNX_Q96FZ7.
OpenTargetsiENSG00000176108.
PharmGKBiPA142672114.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2910. Eukaryota.
ENOG4111HN3. LUCA.
GeneTreeiENSGT00720000108863.
HOGENOMiHOG000208642.
HOVERGENiHBG080510.
InParanoidiQ96FZ7.
KOiK12195.
OMAiRKCLQQG.
PhylomeDBiQ96FZ7.
TreeFamiTF105929.

Enzyme and pathway databases

ReactomeiR-HSA-162588. Budding and maturation of HIV virion.
R-HSA-1632852. Macroautophagy.
R-HSA-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

EvolutionaryTraceiQ96FZ7.
GeneWikiiCHMP6.
GenomeRNAii79643.
PROiQ96FZ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000176108.
CleanExiHS_CHMP6.
ExpressionAtlasiQ96FZ7. baseline and differential.
GenevisibleiQ96FZ7. HS.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHMP6_HUMAN
AccessioniPrimary (citable) accession number: Q96FZ7
Secondary accession number(s): A8K7U0, Q53FU4, Q9HAE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.