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Protein

tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A

Gene

TRMT61A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA (PubMed:16043508). Catalytic subunit of mRNA N1-methyltransferase complex, which mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries (PubMed:29107537, PubMed:29072297).3 Publications

Catalytic activityi

S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N1-methyladenine(58) in tRNA.PROSITE-ProRule annotation1 Publication
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N1-methyladenine in mRNA.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87S-adenosyl-L-methionine; via amide nitrogenCombined sources1 Publication1
Binding sitei135S-adenosyl-L-methioninePROSITE-ProRule annotationCombined sources1 Publication1
Binding sitei140S-adenosyl-L-methionineCombined sources1 Publication1
Binding sitei181S-adenosyl-L-methioninePROSITE-ProRule annotationCombined sources1 Publication1
Binding sitei278SubstrateCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

  • mRNA methylation Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.220 2681
ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A (EC:2.1.1.2201 Publication)
Alternative name(s):
mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A1 Publication (EC:2.1.1.-2 Publications)
tRNA(m1A58)-methyltransferase subunit TRMT61A
Short name:
tRNA(m1A58)MTase subunit TRMT61A
Gene namesi
Name:TRMT61A
Synonyms:C14orf172, TRM61
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000166166.12
HGNCiHGNC:23790 TRMT61A
neXtProtiNX_Q96FX7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi115708
OpenTargetsiENSG00000166166
PharmGKBiPA164726783

Polymorphism and mutation databases

BioMutaiTRMT61A
DMDMi74760827

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002330942 – 289tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61AAdd BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei263PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96FX7
MaxQBiQ96FX7
PaxDbiQ96FX7
PeptideAtlasiQ96FX7
PRIDEiQ96FX7

PTM databases

iPTMnetiQ96FX7
PhosphoSitePlusiQ96FX7

Expressioni

Gene expression databases

BgeeiENSG00000166166
ExpressionAtlasiQ96FX7 baseline and differential
GenevisibleiQ96FX7 HS

Interactioni

Subunit structurei

Heterotetramer; composed of two copies of TRMT6 and two copies of TRMT61A.1 Publication1 Publication

Protein-protein interaction databases

BioGridi125450, 26 interactors
IntActiQ96FX7, 7 interactors
MINTiQ96FX7
STRINGi9606.ENSP00000374399

Structurei

Secondary structure

1289
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Beta strandi15 – 19Combined sources5
Beta strandi25 – 29Combined sources5
Beta strandi35 – 38Combined sources4
Beta strandi41 – 44Combined sources4
Helixi45 – 48Combined sources4
Turni49 – 51Combined sources3
Beta strandi57 – 59Combined sources3
Beta strandi61 – 63Combined sources3
Beta strandi65 – 69Combined sources5
Helixi73 – 77Combined sources5
Helixi89 – 98Combined sources10
Beta strandi106 – 110Combined sources5
Helixi116 – 125Combined sources10
Turni126 – 128Combined sources3
Beta strandi130 – 134Combined sources5
Helixi138 – 150Combined sources13
Helixi154 – 156Combined sources3
Beta strandi157 – 160Combined sources4
Helixi164 – 167Combined sources4
Beta strandi175 – 180Combined sources6
Helixi185 – 188Combined sources4
Helixi189 – 195Combined sources7
Beta strandi201 – 209Combined sources9
Helixi210 – 223Combined sources14
Beta strandi226 – 243Combined sources18
Beta strandi265 – 273Combined sources9
Beta strandi280 – 286Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CCBX-ray2.00A1-289[»]
5CCXX-ray2.10A1-289[»]
5CD1X-ray3.60A/D1-289[»]
ProteinModelPortaliQ96FX7
SMRiQ96FX7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 22SubstrateCombined sources1 Publication3
Regioni35 – 42SubstrateCombined sources1 Publication8
Regioni64 – 65SubstrateCombined sources1 Publication2
Regioni85 – 89SubstrateCombined sources1 Publication5
Regioni110 – 117SubstrateCombined sources1 Publication8
Regioni114 – 116S-adenosyl-L-methionine bindingCombined sources1 Publication3
Regioni163 – 164S-adenosyl-L-methionine bindingCombined sources1 Publication2
Regioni180 – 183SubstrateCombined sources1 Publication4
Regioni205 – 212SubstrateCombined sources1 Publication8

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2915 Eukaryota
COG2519 LUCA
GeneTreeiENSGT00510000048063
HOGENOMiHOG000173282
HOVERGENiHBG061059
InParanoidiQ96FX7
KOiK07442
OMAiVVYPKDA
OrthoDBiEOG091G0BHV
PhylomeDBiQ96FX7
TreeFamiTF315053

Family and domain databases

InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR014816 tRNA_MeTrfase_Gcd14
PANTHERiPTHR12133 PTHR12133, 1 hit
PfamiView protein in Pfam
PF08704 GCD14, 1 hit
PIRSFiPIRSF017269 GCD14, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51620 SAM_TRM61, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96FX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFVAYEELI KEGDTAILSL GHGAMVAVRV QRGAQTQTRH GVLRHSVDLI
60 70 80 90 100
GRPFGSKVTC GRGGWVYVLH PTPELWTLNL PHRTQILYST DIALITMMLE
110 120 130 140 150
LRPGSVVCES GTGSGSVSHA IIRTIAPTGH LHTVEFHQQR AEKAREEFQE
160 170 180 190 200
HRVGRWVTVR TQDVCRSGFG VSHVADAVFL DIPSPWEAVG HAWDALKVEG
210 220 230 240 250
GRFCSFSPCI EQVQRTCQAL AARGFSELST LEVLPQVYNV RTVSLPPPDL
260 270 280
GTGTDGPAGS DTSPFRSGTP MKEAVGHTGY LTFATKTPG
Length:289
Mass (Da):31,382
Last modified:December 1, 2001 - v1
Checksum:i5DCD6437A0B6F54D
GO

Sequence cautioni

The sequence BAC05168 differs from that shown. Probable cloning artifact.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02605366V → A1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133367 Genomic DNA No translation available.
AL139300 Genomic DNA No translation available.
BC010167 mRNA Translation: AAH10167.1
AK097771 mRNA Translation: BAC05168.1 Sequence problems.
CCDSiCCDS41994.1
RefSeqiNP_689520.2, NM_152307.2
UniGeneiHs.525610

Genome annotation databases

EnsembliENST00000389749; ENSP00000374399; ENSG00000166166
GeneIDi115708
KEGGihsa:115708
UCSCiuc001yng.4 human

Keywords - Coding sequence diversityi

Polymorphism

Entry informationi

Entry nameiTRM61_HUMAN
AccessioniPrimary (citable) accession number: Q96FX7
Secondary accession number(s): A6NN78, Q8N7Q9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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