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Protein

DPH3 homolog

Gene

DPH3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in elongation factor 2 (EEF2) and which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta).By similarity
Down-regulation increases extracellular release of proteoglycans, indicating a possible role in the secretion process. Stimulates binding of GNEFR to SEC5.2 Publications

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri4 – 6057DPH-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • negative regulation of protein secretion Source: UniProtKB
  • peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: UniProtKB
  • positive regulation of binding Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-5358493. Synthesis of diphthamide-EEF2.
UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
DPH3 homolog
Alternative name(s):
CSL-type zinc finger-containing protein 2
DelGEF-interacting protein 1
Short name:
DelGIP1
Gene namesi
Name:DPH3
Synonyms:DESR1, ZCSL2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:27717. DPH3.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162384061.

Polymorphism and mutation databases

BioMutaiDPH3.
DMDMi29611922.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8282DPH3 homologPRO_0000082620Add
BLAST

Proteomic databases

MaxQBiQ96FX2.
PaxDbiQ96FX2.
PRIDEiQ96FX2.

Expressioni

Tissue specificityi

Widely expressed with highest levels in small intestine, spleen, thymus, heart, liver and lung.2 Publications

Gene expression databases

BgeeiQ96FX2.
CleanExiHS_DPH3.
ExpressionAtlasiQ96FX2. baseline and differential.
GenevisibleiQ96FX2. HS.

Organism-specific databases

HPAiHPA035287.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PIH1D2Q8WWB53EBI-465363,EBI-10232538
SERGEFQ9UGK83EBI-465363,EBI-465368

Protein-protein interaction databases

BioGridi130097. 8 interactions.
IntActiQ96FX2. 4 interactions.
STRINGi9606.ENSP00000419599.

Structurei

Secondary structure

1
82
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93Combined sources
Turni10 – 123Combined sources
Beta strandi13 – 153Combined sources
Turni17 – 193Combined sources
Beta strandi20 – 256Combined sources
Beta strandi29 – 357Combined sources
Helixi36 – 416Combined sources
Beta strandi45 – 473Combined sources
Turni49 – 513Combined sources
Beta strandi54 – 585Combined sources
Helixi61 – 644Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JR7NMR-A1-81[»]
ProteinModelPortaliQ96FX2.
SMRiQ96FX2. Positions 1-81.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96FX2.

Family & Domainsi

Sequence similaritiesi

Belongs to the DPH3 family.Curated
Contains 1 DPH-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri4 – 6057DPH-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2923. Eukaryota.
COG5216. LUCA.
GeneTreeiENSGT00390000007225.
HOGENOMiHOG000190740.
HOVERGENiHBG056007.
InParanoidiQ96FX2.
KOiK15455.
OMAiREMLEMG.
OrthoDBiEOG7BZVWK.
PhylomeDBiQ96FX2.
TreeFamiTF315102.

Family and domain databases

InterProiIPR007872. Znf_DHP.
[Graphical view]
PfamiPF05207. zf-CSL. 1 hit.
[Graphical view]
PROSITEiPS51074. ZF_DPH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96FX2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVFHDEVEI EDFQYDEDSE TYFYPCPCGD NFSITKEDLE NGEDVATCPS
60 70 80
CSLIIKVIYD KDQFVCGETV PAPSANKELV KC
Length:82
Mass (Da):9,240
Last modified:December 1, 2001 - v1
Checksum:i7AC8F3FFF8CE766C
GO
Isoform 2 (identifier: Q96FX2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-60: Missing.

Note: No experimental confirmation available.
Show »
Length:57
Mass (Da):6,503
Checksum:iB26EFCD8DA36D558
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei36 – 6025Missing in isoform 2. 1 PublicationVSP_012411Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK022970 mRNA. No translation available.
BC010181 mRNA. Translation: AAH10181.1.
CCDSiCCDS2629.1. [Q96FX2-1]
CCDS43058.1. [Q96FX2-2]
RefSeqiNP_001040899.1. NM_001047434.2. [Q96FX2-2]
NP_996662.1. NM_206831.2. [Q96FX2-1]
UniGeneiHs.388087.

Genome annotation databases

EnsembliENST00000383775; ENSP00000373285; ENSG00000154813. [Q96FX2-2]
ENST00000488423; ENSP00000419599; ENSG00000154813. [Q96FX2-1]
GeneIDi285381.
KEGGihsa:285381.
UCSCiuc003cau.4. human. [Q96FX2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK022970 mRNA. No translation available.
BC010181 mRNA. Translation: AAH10181.1.
CCDSiCCDS2629.1. [Q96FX2-1]
CCDS43058.1. [Q96FX2-2]
RefSeqiNP_001040899.1. NM_001047434.2. [Q96FX2-2]
NP_996662.1. NM_206831.2. [Q96FX2-1]
UniGeneiHs.388087.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JR7NMR-A1-81[»]
ProteinModelPortaliQ96FX2.
SMRiQ96FX2. Positions 1-81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130097. 8 interactions.
IntActiQ96FX2. 4 interactions.
STRINGi9606.ENSP00000419599.

Polymorphism and mutation databases

BioMutaiDPH3.
DMDMi29611922.

Proteomic databases

MaxQBiQ96FX2.
PaxDbiQ96FX2.
PRIDEiQ96FX2.

Protocols and materials databases

DNASUi285381.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000383775; ENSP00000373285; ENSG00000154813. [Q96FX2-2]
ENST00000488423; ENSP00000419599; ENSG00000154813. [Q96FX2-1]
GeneIDi285381.
KEGGihsa:285381.
UCSCiuc003cau.4. human. [Q96FX2-1]

Organism-specific databases

CTDi285381.
GeneCardsiDPH3.
HGNCiHGNC:27717. DPH3.
HPAiHPA035287.
MIMi608959. gene.
neXtProtiNX_Q96FX2.
PharmGKBiPA162384061.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2923. Eukaryota.
COG5216. LUCA.
GeneTreeiENSGT00390000007225.
HOGENOMiHOG000190740.
HOVERGENiHBG056007.
InParanoidiQ96FX2.
KOiK15455.
OMAiREMLEMG.
OrthoDBiEOG7BZVWK.
PhylomeDBiQ96FX2.
TreeFamiTF315102.

Enzyme and pathway databases

UniPathwayiUPA00559.
ReactomeiR-HSA-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

EvolutionaryTraceiQ96FX2.
GenomeRNAii285381.
PROiQ96FX2.
SOURCEiSearch...

Gene expression databases

BgeeiQ96FX2.
CleanExiHS_DPH3.
ExpressionAtlasiQ96FX2. baseline and differential.
GenevisibleiQ96FX2. HS.

Family and domain databases

InterProiIPR007872. Znf_DHP.
[Graphical view]
PfamiPF05207. zf-CSL. 1 hit.
[Graphical view]
PROSITEiPS51074. ZF_DPH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterisation of an evolutionary conserved protein interacting with the putative guanine nucleotide exchange factor DelGEF and modulating secretion."
    Sjoelinder M., Uhlmann J., Ponstingl H.
    Exp. Cell Res. 294:68-76(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH GNEFR.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Teratocarcinoma.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  4. "Retroviral insertional mutagenesis identifies a small protein required for synthesis of diphthamide, the target of bacterial ADP-ribosylating toxins."
    Liu S., Leppla S.H.
    Mol. Cell 12:603-613(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "Solution structure of human DESR1, a CSL zinc-binding protein."
    Wu F., Zhang J., Sun J., Huang H., Ji P., Chu W., Yu M., Yang F., Wu Z., Wu J., Shi Y.
    Proteins 71:514-518(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-81 IN COMPLEX WITH ZINC IONS.

Entry informationi

Entry nameiDPH3_HUMAN
AccessioniPrimary (citable) accession number: Q96FX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2003
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.