Reviewed, UniProtKB/Swiss-Prot Q96FX2 (DPH3_HUMAN)

Names and origin

Protein names

Recommended name:
    DPH3 homolog
Alternative name(s):
    CSL-type zinc finger-containing protein 2
    DelGEF-interacting protein 1
      Short name=DelGIP1

Gene names

Name:	DPH3
Synonyms:	DESR1, ZCSL2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	82 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in elongation factor 2 and which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A By similarity. Down-regulation increases extracellular release of proteoglycans, indicating a possible role in the secretion process. Stimulates binding of GNEFR to SEC5. Ref.1 Ref.4
Subcellular location	Cytoplasm. Nucleus. Ref.1
Tissue specificity	Widely expressed with highest levels in small intestine, spleen, thymus, heart, liver and lung. Ref.1 Ref.4
Sequence similarities	Belongs to the DPH3 family. Contains 1 DPH-type zinc finger.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure
Gene Ontology (GO)
Biological process	negative regulation of protein secretion Ref.1 Inferred from direct assay. Source: UniProtKB peptidyl-diphthamide biosynthetic process from peptidyl-histidine Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of binding Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: UniProtKB nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SERGEF	Q9UGK8	3	EBI-465363,EBI-465368

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96FX2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96FX2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     36-60: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 82

82

DPH3 homolog

PRO_0000082620

Regions

Zinc finger

4 – 60

57

DPH-type

Natural variations

Alternative sequence

36 – 60

25

Missing in isoform 2.

VSP_012411

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

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.

.

.

.

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82

Helix Strand Turn

Details...

Beta strand

7 – 9

3

Turn

10 – 12

3

Beta strand

13 – 15

3

Turn

17 – 19

3

Beta strand

20 – 25

6

Beta strand

29 – 35

7

Helix

36 – 41

6

Beta strand

45 – 47

3

Turn

49 – 51

3

Beta strand

54 – 58

5

Helix

61 – 64

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7AC8F3FFF8CE766C	FASTA	82	9,240
	10 20 30 40 50 60 MAVFHDEVEI EDFQYDEDSE TYFYPCPCGD NFSITKEDLE NGEDVATCPS CSLIIKVIYD 70 80 KDQFVCGETV PAPSANKELV KC
	Isoform 2. Checksum: B26EFCD8DA36D558	FASTA	57	6,503
	10 20 30 40 50 MAVFHDEVEI EDFQYDEDSE TYFYPCPCGD NFSITKDQFV CGETVPAPSA NKELVKC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterisation of an evolutionary conserved protein interacting with the putative guanine nucleotide exchange factor DelGEF and modulating secretion." Sjoelinder M., Uhlmann J., Ponstingl H. Exp. Cell Res. 294:68-76(2004) [PubMed: 14980502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH GNEFR.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Teratocarcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin.
[4]	"Retroviral insertional mutagenesis identifies a small protein required for synthesis of diphthamide, the target of bacterial ADP-ribosylating toxins." Liu S., Leppla S.H. Mol. Cell 12:603-613(2003) [PubMed: 14527407] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AK022970 mRNA. No translation available.
BC010181 mRNA. Translation: AAH10181.1.

IPI

IPI00401015.
IPI00514553.

RefSeq

NP_001040899.1.
NP_996662.1.

UniGene

Hs.388087

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JR7	NMR	-	A	1-81	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96FX2. 1 interaction.

Proteomic databases

PRIDE

Q96FX2.

Genome annotation databases

Ensembl

ENSG00000154813. Homo sapiens. [Contig view]

GeneID

285381.

KEGG

hsa:285381.

Organism-specific databases

GeneCards

GC03M016277.

H-InvDB

HIX0003109.

HGNC

HGNC:27717. DPH3.

MIM

608959. gene.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q96FX2.

OMA

Q96FX2. IEDFEYD.

Gene expression databases

ArrayExpress

Q96FX2.

Bgee

Q96FX2.

CleanEx

HS_DPH3.

GermOnline

ENSG00000154813. Homo sapiens.

Family and domain databases

InterPro

IPR007872. Znf_DHP.
[Graphical view]

Pfam

PF05207. zf-CSL. 1 hit.
[Graphical view]

PROSITE

PS51074. ZF_DPH. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

95485.

SOURCE

Search...

Entry information

Entry name

DPH3_HUMAN

Accession

Primary (citable) accession number: Q96FX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)