Q96FQ6 (S10AG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A16

Alternative name(s):
Aging-associated gene 13 protein
Protein S100-F
S100 calcium-binding protein A16

Gene names

Name:	S100A16
Synonyms:	S100F
ORF Names:	AAG13

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	103 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-binding protein. Binds one calcium ion per monomer. Ref.9
Subunit structure	Homodimer. Ref.9 Ref.10
Subcellular location	Nucleus › nucleolus. Cytoplasm. Note: Primarily nucleolar. Translocates from the nucleolus to the cytoplasm in response to an increase of intracellular calcium levels. Ref.9
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	Calcium Metal-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytosol Inferred from direct assay Ref.9. Source: UniProtKB nucleolus Inferred from direct assay Ref.9. Source: UniProtKB plasma membrane Inferred from direct assay. Source: HPA
Molecular function	calcium ion binding Inferred from direct assay Ref.9. Source: UniProtKB protein homodimerization activity Inferred from physical interaction Ref.9. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 103

103

Protein S100-A16

PRO_0000144023

Regions

Domain

12 – 47

36

EF-hand 1

Domain

54 – 89

36

EF-hand 2

Calcium binding

23 – 36

14

1; low affinity Potential

Calcium binding

67 – 78

12

2; high affinity Ref.9 Ref.10

Amino acid modifications

Modified residue

103

1

Phosphoserine Ref.8

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

103

Helix Strand Turn

Details...

Helix

7 – 21

15

Helix

35 – 45

11

Turn

47 – 50

4

Helix

56 – 65

10

Helix

76 – 82

7

Helix

91 – 100

10

Sequences

	Sequence		Length	Mass (Da)
	Q96FQ6 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7D00C08F85697A6C	FASTA	103	11,801
	10 20 30 40 50 60 MSDCYTELEK AVIVLVENFY KYVSKYSLVK NKISKSSFRE MLQKELNHML SDTGNRKAAD 70 80 90 100 KLIQNLDANH DGRISFDEYW TLIGGITGPI AKLIHEQEQQ SSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"S100A16, a new EF-hand protein and potential tumor promoter in human." Marenholz I., Heizmann C.W. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Morita K. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Keratinocyte.
[3]	"Identification of a human aging related gene." Kim J.W. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Cervix.
[8]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"S100A16, a novel calcium-binding protein of the EF-hand superfamily." Sturchler E., Cox J.A., Durussel I., Weibel M., Heizmann C.W. J. Biol. Chem. 281:38905-38917(2006) [PubMed: 17030513] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MASS SPECTROMETRY, CALCIUM-BINDING.
[10]	"Structural characterization of human S100A16, a low-affinity calcium binder." Babini E., Bertini I., Borsi V., Calderone V., Hu X., Luchinat C., Parigi G. J. Biol. Inorg. Chem. 16:243-256(2011) [PubMed: 21046186] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), STRUCTURE BY NMR, SUBUNIT, CALCIUM-BINDING DOMAIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ585980 Genomic DNA. Translation: CAE51865.1.
AY221961 mRNA. Translation: AAP46152.1.
AY762098 mRNA. Translation: AAW88319.1.
AK290804 mRNA. Translation: BAF83493.1.
BX470102 Genomic DNA. Translation: CAI14758.1.
CH471121 Genomic DNA. Translation: EAW53303.1.
CH471121 Genomic DNA. Translation: EAW53304.1.
BC010541 mRNA. Translation: AAH10541.1.
BC019099 mRNA. Translation: AAH19099.1.
BC095462 mRNA. Translation: AAH95462.1.

IPI

IPI00062120.

RefSeq

NP_525127.1. NM_080388.1.

UniGene

Hs.515714.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L50	NMR	-	A/B	2-103	[»]
2L51	NMR	-	A/B	2-103	[»]
3NXA	X-ray	2.10	A/B/C/D	3-102	[»]

ProteinModelPortal

Q96FQ6.

SMR

Q96FQ6. Positions 13-86.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96FQ6. 3 interactions.

MINT

MINT-1457417.

STRING

Q96FQ6.

PTM databases

PhosphoSite

Q96FQ6.

Polymorphism databases

DMDM

20178113.

2D gel databases

SWISS-2DPAGE

Q96FQ6.

Proteomic databases

PeptideAtlas

Q96FQ6.

PRIDE

Q96FQ6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000368703; ENSP00000357692; ENSG00000188643.
ENST00000368704; ENSP00000357693; ENSG00000188643.
ENST00000368705; ENSP00000357694; ENSG00000188643.
ENST00000368706; ENSP00000357695; ENSG00000188643.

GeneID

140576.

KEGG

hsa:140576.

UCSC

uc001fcc.2. human.

Organism-specific databases

CTD

140576.

GeneCards

GC01M153579.

H-InvDB

HIX0199964.

HGNC

HGNC:20441. S100A16.

HPA

HPA045841.

neXtProt

NX_Q96FQ6.

PharmGKB

PA134931606.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21016.

GeneTree

ENSGT00390000000920.

HOGENOM

HBG126259.

HOVERGEN

HBG001479.

InParanoid

Q96FQ6.

OMA

ITGPIAN.

OrthoDB

EOG4W0XFM.

PhylomeDB

Q96FQ6.

Gene expression databases

ArrayExpress

Q96FQ6.

Bgee

Q96FQ6.

CleanEx

HS_S100A16.

Genevestigator

Q96FQ6.

GermOnline

ENSG00000188643. Homo sapiens.

Family and domain databases

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

Pfam

PF01023. S_100. 1 hit.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

84165.

Entry information

Entry name

S10AG_HUMAN

Accession

Primary (citable) accession number: Q96FQ6
Secondary accession number(s): A8K439, D3DV52, Q5RHS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.