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Protein

Copine-2

Gene

CPNE2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Exhibits calcium-dependent cell membrane binding properties.By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Copine-2Curated
Alternative name(s):
Copine IIBy similarityImported
Gene namesi
Name:CPNE2Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:2315. CPNE2.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Cell membrane By similarity

  • Note: Translocates to the cell membrane and the nucleus in a calcium-dependent manner. Colocalizes with CD2 at the cell membrane.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26832.

Polymorphism and mutation databases

BioMutaiCPNE2.
DMDMi33860150.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548Copine-2PRO_0000144836Add
BLAST

Proteomic databases

EPDiQ96FN4.
MaxQBiQ96FN4.
PaxDbiQ96FN4.
PRIDEiQ96FN4.

PTM databases

iPTMnetiQ96FN4.
PhosphoSiteiQ96FN4.

Expressioni

Tissue specificityi

Expressed in the brain. Expressed in neutrophil precursors from the bone marrow and peripheral blood (PubMed:12949241).

Gene expression databases

BgeeiQ96FN4.
CleanExiHS_CPNE2.
ExpressionAtlasiQ96FN4. baseline and differential.
GenevisibleiQ96FN4. HS.

Organism-specific databases

HPAiHPA041132.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LNX1Q8TBB13EBI-7097057,EBI-739832
RELQ048643EBI-7097057,EBI-307352
SORBS3O605043EBI-7097057,EBI-741237
TERF2IPQ9NYB02EBI-7097057,EBI-750109
TRIM27P143733EBI-7097057,EBI-719493
UBA3Q8TBC43EBI-7097057,EBI-717567

Protein-protein interaction databases

BioGridi128695. 59 interactions.
IntActiQ96FN4. 12 interactions.
MINTiMINT-8049255.
STRINGi9606.ENSP00000290776.

Structurei

3D structure databases

ProteinModelPortaliQ96FN4.
SMRiQ96FN4. Positions 163-255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 115106C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini141 – 246106C2 2PROSITE-ProRule annotationAdd
BLAST
Domaini305 – 507203VWFAPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni247 – 30458Linker regionBy similarityAdd
BLAST

Domaini

The C2 domain 2, but not the C2 domain 1, is necessary for calcium-mediated membrane association. The linker region is necessary for calcium-dependent cell membrane association.By similarity

Sequence similaritiesi

Belongs to the copine family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1327. Eukaryota.
ENOG410XPC8. LUCA.
GeneTreeiENSGT00760000119085.
HOGENOMiHOG000220898.
HOVERGENiHBG066841.
InParanoidiQ96FN4.
OMAiQDKSSMQ.
OrthoDBiEOG77DJ57.
PhylomeDBiQ96FN4.
TreeFamiTF316419.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR010734. Copine.
IPR002035. VWF_A.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF07002. Copine. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF53300. SSF53300. 1 hit.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96FN4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAHIPSGGAP AAGAAPMGPQ YCVCKVELSV SGQNLLDRDV TSKSDPFCVL
60 70 80 90 100
FTENNGRWIE YDRTETAINN LNPAFSKKFV LDYHFEEVQK LKFALFDQDK
110 120 130 140 150
SSMRLDEHDF LGQFSCSLGT IVSSKKITRP LLLLNDKPAG KGLITIAAQE
160 170 180 190 200
LSDNRVITLS LAGRRLDKKD LFGKSDPFLE FYKPGDDGKW MLVHRTEVIK
210 220 230 240 250
YTLDPVWKPF TVPLVSLCDG DMEKPIQVMC YDYDNDGGHD FIGEFQTSVS
260 270 280 290 300
QMCEARDSVP LEFECINPKK QRKKKNYKNS GIIILRSCKI NRDYSFLDYI
310 320 330 340 350
LGGCQLMFTV GIDFTASNGN PLDPSSLHYI NPMGTNEYLS AIWAVGQIIQ
360 370 380 390 400
DYDSDKMFPA LGFGAQLPPD WKVSHEFAIN FNPTNPFCSG VDGIAQAYSA
410 420 430 440 450
CLPHIRFYGP TNFSPIVNHV ARFAAQATQQ RTATQYFILL IITDGVISDM
460 470 480 490 500
EETRHAVVQA SKLPMSIIIV GVGNADFAAM EFLDGDSRML RSHTGEEAAR
510 520 530 540
DIVQFVPFRE FRNAAKETLA KAVLAELPQQ VVQYFKHKNL PPTNSEPA
Length:548
Mass (Da):61,190
Last modified:August 15, 2003 - v3
Checksum:i39B962C82C591DA0
GO
Isoform 2 (identifier: Q96FN4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.

Show »
Length:446
Mass (Da):49,890
Checksum:iF1B5EB45B81781ED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti100 – 1001K → E in CAH18411 (PubMed:17974005).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 102102Missing in isoform 2. 1 PublicationVSP_055536Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492484 mRNA. Translation: AAO85483.1.
AF542551 mRNA. Translation: AAQ09534.1.
AC009090 Genomic DNA. No translation available.
BC053658 mRNA. Translation: AAH53658.1.
CR749617 mRNA. Translation: CAH18411.1.
CCDSiCCDS10774.1. [Q96FN4-1]
RefSeqiNP_689940.3. NM_152727.5. [Q96FN4-1]
UniGeneiHs.339809.
Hs.736510.

Genome annotation databases

EnsembliENST00000290776; ENSP00000290776; ENSG00000140848. [Q96FN4-1]
ENST00000535318; ENSP00000439018; ENSG00000140848. [Q96FN4-1]
ENST00000565874; ENSP00000456042; ENSG00000140848. [Q96FN4-1]
GeneIDi221184.
KEGGihsa:221184.
UCSCiuc002eks.3. human. [Q96FN4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492484 mRNA. Translation: AAO85483.1.
AF542551 mRNA. Translation: AAQ09534.1.
AC009090 Genomic DNA. No translation available.
BC053658 mRNA. Translation: AAH53658.1.
CR749617 mRNA. Translation: CAH18411.1.
CCDSiCCDS10774.1. [Q96FN4-1]
RefSeqiNP_689940.3. NM_152727.5. [Q96FN4-1]
UniGeneiHs.339809.
Hs.736510.

3D structure databases

ProteinModelPortaliQ96FN4.
SMRiQ96FN4. Positions 163-255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128695. 59 interactions.
IntActiQ96FN4. 12 interactions.
MINTiMINT-8049255.
STRINGi9606.ENSP00000290776.

PTM databases

iPTMnetiQ96FN4.
PhosphoSiteiQ96FN4.

Polymorphism and mutation databases

BioMutaiCPNE2.
DMDMi33860150.

Proteomic databases

EPDiQ96FN4.
MaxQBiQ96FN4.
PaxDbiQ96FN4.
PRIDEiQ96FN4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000290776; ENSP00000290776; ENSG00000140848. [Q96FN4-1]
ENST00000535318; ENSP00000439018; ENSG00000140848. [Q96FN4-1]
ENST00000565874; ENSP00000456042; ENSG00000140848. [Q96FN4-1]
GeneIDi221184.
KEGGihsa:221184.
UCSCiuc002eks.3. human. [Q96FN4-1]

Organism-specific databases

CTDi221184.
GeneCardsiCPNE2.
HGNCiHGNC:2315. CPNE2.
HPAiHPA041132.
MIMi604206. gene.
neXtProtiNX_Q96FN4.
PharmGKBiPA26832.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1327. Eukaryota.
ENOG410XPC8. LUCA.
GeneTreeiENSGT00760000119085.
HOGENOMiHOG000220898.
HOVERGENiHBG066841.
InParanoidiQ96FN4.
OMAiQDKSSMQ.
OrthoDBiEOG77DJ57.
PhylomeDBiQ96FN4.
TreeFamiTF316419.

Miscellaneous databases

ChiTaRSiCPNE2. human.
GenomeRNAii221184.
NextBioi91227.
PROiQ96FN4.
SOURCEiSearch...

Gene expression databases

BgeeiQ96FN4.
CleanExiHS_CPNE2.
ExpressionAtlasiQ96FN4. baseline and differential.
GenevisibleiQ96FN4. HS.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR010734. Copine.
IPR002035. VWF_A.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF07002. Copine. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF53300. SSF53300. 1 hit.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Ding P., Han W., Shi S., Rui M., Wang Y., Song Q., Ma D.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Placenta.
  2. "Cloning and characterization of a novel human gene encoding a protein of 446 amino acids."
    Zhang D.L., Cai J.J., Ma D.L.
    Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pancreas.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 20-548 (ISOFORM 1).
    Tissue: Fetal brain.
  6. "Tissue expression of copines and isolation of copines I and III from the cytosol of human neutrophils."
    Cowland J.B., Carter D., Bjerregaard M.D., Johnsen A.H., Borregaard N., Lollike K.
    J. Leukoc. Biol. 74:379-388(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCPNE2_HUMAN
AccessioniPrimary (citable) accession number: Q96FN4
Secondary accession number(s): Q68D19, Q719H8, Q86XP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: August 15, 2003
Last modified: April 13, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.