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Protein

Multidrug and toxin extrusion protein 1

Gene

SLC47A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes.5 Publications

Kineticsi

  1. KM=0.38 mM for TEA2 Publications
  2. KM=0.10 mM for MPP2 Publications
  3. KM=0.17 mM for cimetidine2 Publications
  4. KM=0.78 mM for metformin2 Publications
  5. KM=2.1 mM for guanidine2 Publications
  6. KM=1.23 mM for procainamide2 Publications
  7. KM=0.07 mM for topotecan2 Publications
  8. KM=0.47 mM for estrone sulfate2 Publications
  9. KM=2.64 mM for acyclovir2 Publications
  10. KM=5.12 mM for ganciclovir2 Publications
  1. Vmax=1.185 nmol/min/mg enzyme toward TEA2 Publications
  2. Vmax=0.735 nmol/min/mg enzyme toward MPP2 Publications
  3. Vmax=0.135 nmol/min/mg enzyme toward cimetidine2 Publications
  4. Vmax=2.23 nmol/min/mg enzyme toward metformin2 Publications
  5. Vmax=0.89 nmol/min/mg enzyme toward guanidine2 Publications
  6. Vmax=3.78 nmol/min/mg enzyme toward procainamide2 Publications
  7. Vmax=0.21 nmol/min/mg enzyme toward topotecan2 Publications
  8. Vmax=0.265 nmol/min/mg enzyme toward estrone sulfate2 Publications
  9. Vmax=0.62 nmol/min/mg enzyme toward acyclovir2 Publications
  10. Vmax=1.08 nmol/min/mg enzyme toward ganciclovir2 Publications

pH dependencei

Optimum pH is 8.5. Active from pH 6 to 8.5.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000142494-MONOMER.
ReactomeiR-HSA-425366. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Protein family/group databases

TCDBi2.A.66.1.14. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug and toxin extrusion protein 1
Short name:
MATE-1
Short name:
hMATE-1
Alternative name(s):
Solute carrier family 47 member 1
Gene namesi
Name:SLC47A1
Synonyms:MATE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:25588. SLC47A1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Topological domaini59 – 72ExtracellularSequence analysisAdd BLAST14
Transmembranei73 – 93HelicalSequence analysisAdd BLAST21
Topological domaini94 – 123CytoplasmicSequence analysisAdd BLAST30
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 152ExtracellularSequence analysis8
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 176CytoplasmicSequence analysis3
Transmembranei177 – 197HelicalSequence analysisAdd BLAST21
Topological domaini198 – 216ExtracellularSequence analysisAdd BLAST19
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Topological domaini238 – 256CytoplasmicSequence analysisAdd BLAST19
Transmembranei257 – 276HelicalSequence analysisAdd BLAST20
Topological domaini277 – 295ExtracellularSequence analysisAdd BLAST19
Transmembranei296 – 316HelicalSequence analysisAdd BLAST21
Topological domaini317 – 336CytoplasmicSequence analysisAdd BLAST20
Transmembranei337 – 357HelicalSequence analysisAdd BLAST21
Topological domaini358 – 370ExtracellularSequence analysisAdd BLAST13
Transmembranei371 – 391HelicalSequence analysisAdd BLAST21
Topological domaini392 – 408CytoplasmicSequence analysisAdd BLAST17
Transmembranei409 – 429HelicalSequence analysisAdd BLAST21
Topological domaini430 – 437ExtracellularSequence analysis8
Transmembranei438 – 458HelicalSequence analysisAdd BLAST21
Topological domaini459 – 546CytoplasmicSequence analysisAdd BLAST88
Transmembranei547 – 567HelicalSequence analysisAdd BLAST21
Topological domaini568 – 570ExtracellularSequence analysis3

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi273E → Q: No change in subcellular location and abolition of MATE1-dependent TEA transport activity. 1 Publication1

Organism-specific databases

DisGeNETi55244.
OpenTargetsiENSG00000142494.
PharmGKBiPA162403808.

Chemistry databases

ChEMBLiCHEMBL1743126.
DrugBankiDB00787. Aciclovir.
DB01237. Bromodiphenhydramine.
DB00567. Cephalexin.
DB00501. Cimetidine.
DB00199. Erythromycin.
DB00331. Metformin.
DB00877. Sirolimus.
GuidetoPHARMACOLOGYi1216.

Polymorphism and mutation databases

BioMutaiSLC47A1.
DMDMi74731723.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003128451 – 570Multidrug and toxin extrusion protein 1Add BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ96FL8.
PaxDbiQ96FL8.
PeptideAtlasiQ96FL8.
PRIDEiQ96FL8.

PTM databases

PhosphoSitePlusiQ96FL8.

Expressioni

Tissue specificityi

Expressed in adrenal gland, and to a lower extent in liver, skeletal muscle and kidney (especially found in luminal membranes of the urinary tubules, bile caniculi and brush border membranes).2 Publications

Gene expression databases

BgeeiENSG00000142494.
CleanExiHS_SLC47A1.
ExpressionAtlasiQ96FL8. baseline and differential.
GenevisibleiQ96FL8. HS.

Organism-specific databases

HPAiHPA021987.

Interactioni

Protein-protein interaction databases

BioGridi120535. 27 interactors.
STRINGi9606.ENSP00000270570.

Chemistry databases

BindingDBiQ96FL8.

Structurei

3D structure databases

ProteinModelPortaliQ96FL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1347. Eukaryota.
COG0534. LUCA.
GeneTreeiENSGT00390000015713.
HOGENOMiHOG000060313.
HOVERGENiHBG056043.
InParanoidiQ96FL8.
KOiK03327.
OMAiIMRTVIM.
OrthoDBiEOG091G06X2.
PhylomeDBiQ96FL8.
TreeFamiTF324441.

Family and domain databases

InterProiIPR002528. MATE_fam.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96FL8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAPEEPAPV RGGPEATLEV RGSRCLRLSA FREELRALLV LAGPAFLVQL
60 70 80 90 100
MVFLISFISS VFCGHLGKLE LDAVTLAIAV INVTGVSVGF GLSSACDTLI
110 120 130 140 150
SQTYGSQNLK HVGVILQRSA LVLLLCCFPC WALFLNTQHI LLLFRQDPDV
160 170 180 190 200
SRLTQTYVTI FIPALPATFL YMLQVKYLLN QGIVLPQIVT GVAANLVNAL
210 220 230 240 250
ANYLFLHQLH LGVIGSALAN LISQYTLALL LFLYILGKKL HQATWGGWSL
260 270 280 290 300
ECLQDWASFL RLAIPSMLML CMEWWAYEVG SFLSGILGMV ELGAQSIVYE
310 320 330 340 350
LAIIVYMVPA GFSVAASVRV GNALGAGDME QARKSSTVSL LITVLFAVAF
360 370 380 390 400
SVLLLSCKDH VGYIFTTDRD IINLVAQVVP IYAVSHLFEA LACTSGGVLR
410 420 430 440 450
GSGNQKVGAI VNTIGYYVVG LPIGIALMFA TTLGVMGLWS GIIICTVFQA
460 470 480 490 500
VCFLGFIIQL NWKKACQQAQ VHANLKVNNV PRSGNSALPQ DPLHPGCPEN
510 520 530 540 550
LEGILTNDVG KTGEPQSDQQ MRQEEPLPEH PQDGAKLSRK QLVLRRGLLL
560 570
LGVFLILLVG ILVRFYVRIQ
Length:570
Mass (Da):61,922
Last modified:December 1, 2001 - v1
Checksum:iCA940CBC2F6F5CC4
GO
Isoform 2 (identifier: Q96FL8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     286-290: ILGMV → LYEDG
     291-570: Missing.

Note: No experimental confirmation available.
Show »
Length:290
Mass (Da):31,907
Checksum:i662AE04699124D14
GO
Isoform 3 (identifier: Q96FL8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     559-570: VGILVRFYVRIQ → AGVRWCDHSSLQPRTLGLQAILLCQPPE

Note: No experimental confirmation available.
Show »
Length:586
Mass (Da):63,549
Checksum:iA1BF0F64D815E7A8
GO

Sequence cautioni

The sequence AAH50592 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50L → R in BAD96345 (Ref. 2) Curated1
Sequence conflicti309P → L in BAA91852 (PubMed:14702039).Curated1
Sequence conflicti448F → L in BAD96345 (Ref. 2) Curated1
Sequence conflicti507N → D in BAD96345 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037587338V → I.Corresponds to variant rs35790011dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029903286 – 290ILGMV → LYEDG in isoform 2. 1 Publication5
Alternative sequenceiVSP_029904291 – 570Missing in isoform 2. 1 PublicationAdd BLAST280
Alternative sequenceiVSP_029905559 – 570VGILV…YVRIQ → AGVRWCDHSSLQPRTLGLQA ILLCQPPE in isoform 3. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001709 mRNA. Translation: BAA91852.1.
AK222625 mRNA. Translation: BAD96345.1.
CH471212 Genomic DNA. Translation: EAW50893.1.
CH471212 Genomic DNA. Translation: EAW50894.1.
BC010661 mRNA. Translation: AAH10661.1.
BC050592 mRNA. Translation: AAH50592.1. Different initiation.
BC058882 mRNA. Translation: AAH58882.1.
CCDSiCCDS11209.1. [Q96FL8-1]
RefSeqiNP_060712.2. NM_018242.2. [Q96FL8-1]
UniGeneiHs.232054.

Genome annotation databases

EnsembliENST00000270570; ENSP00000270570; ENSG00000142494. [Q96FL8-1]
ENST00000395585; ENSP00000378951; ENSG00000142494. [Q96FL8-3]
GeneIDi55244.
KEGGihsa:55244.
UCSCiuc002gvx.4. human. [Q96FL8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001709 mRNA. Translation: BAA91852.1.
AK222625 mRNA. Translation: BAD96345.1.
CH471212 Genomic DNA. Translation: EAW50893.1.
CH471212 Genomic DNA. Translation: EAW50894.1.
BC010661 mRNA. Translation: AAH10661.1.
BC050592 mRNA. Translation: AAH50592.1. Different initiation.
BC058882 mRNA. Translation: AAH58882.1.
CCDSiCCDS11209.1. [Q96FL8-1]
RefSeqiNP_060712.2. NM_018242.2. [Q96FL8-1]
UniGeneiHs.232054.

3D structure databases

ProteinModelPortaliQ96FL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120535. 27 interactors.
STRINGi9606.ENSP00000270570.

Chemistry databases

BindingDBiQ96FL8.
ChEMBLiCHEMBL1743126.
DrugBankiDB00787. Aciclovir.
DB01237. Bromodiphenhydramine.
DB00567. Cephalexin.
DB00501. Cimetidine.
DB00199. Erythromycin.
DB00331. Metformin.
DB00877. Sirolimus.
GuidetoPHARMACOLOGYi1216.

Protein family/group databases

TCDBi2.A.66.1.14. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

PTM databases

PhosphoSitePlusiQ96FL8.

Polymorphism and mutation databases

BioMutaiSLC47A1.
DMDMi74731723.

Proteomic databases

MaxQBiQ96FL8.
PaxDbiQ96FL8.
PeptideAtlasiQ96FL8.
PRIDEiQ96FL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000270570; ENSP00000270570; ENSG00000142494. [Q96FL8-1]
ENST00000395585; ENSP00000378951; ENSG00000142494. [Q96FL8-3]
GeneIDi55244.
KEGGihsa:55244.
UCSCiuc002gvx.4. human. [Q96FL8-1]

Organism-specific databases

CTDi55244.
DisGeNETi55244.
GeneCardsiSLC47A1.
H-InvDBHIX0013619.
HGNCiHGNC:25588. SLC47A1.
HPAiHPA021987.
MIMi609832. gene.
neXtProtiNX_Q96FL8.
OpenTargetsiENSG00000142494.
PharmGKBiPA162403808.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1347. Eukaryota.
COG0534. LUCA.
GeneTreeiENSGT00390000015713.
HOGENOMiHOG000060313.
HOVERGENiHBG056043.
InParanoidiQ96FL8.
KOiK03327.
OMAiIMRTVIM.
OrthoDBiEOG091G06X2.
PhylomeDBiQ96FL8.
TreeFamiTF324441.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000142494-MONOMER.
ReactomeiR-HSA-425366. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Miscellaneous databases

ChiTaRSiSLC47A1. human.
GeneWikiiSLC47A1.
GenomeRNAii55244.
PROiQ96FL8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000142494.
CleanExiHS_SLC47A1.
ExpressionAtlasiQ96FL8. baseline and differential.
GenevisibleiQ96FL8. HS.

Family and domain databases

InterProiIPR002528. MATE_fam.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS47A1_HUMAN
AccessioniPrimary (citable) accession number: Q96FL8
Secondary accession number(s): Q53HF5
, Q6PD77, Q86VL4, Q9NVA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.