Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q96FL8 (S47A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multidrug and toxin extrusion protein 1

Short name=MATE-1
Short name=hMATE-1
Alternative name(s):
Solute carrier family 47 member 1
Gene names
Name:SLC47A1
Synonyms:MATE1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes. Ref.5 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Predominantly localized at the plasma membrane but also found in intracellular organelles. Ref.5 Ref.6

Tissue specificity

Expressed in adrenal gland, and to a lower extent in liver, skeletal muscle and kidney (especially found in luminal membranes of the urinary tubules, bile caniculi and brush border membranes). Ref.5 Ref.8

Sequence similarities

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=0.38 mM for TEA Ref.5 Ref.8

KM=0.10 mM for MPP

KM=0.17 mM for cimetidine

KM=0.78 mM for metformin

KM=2.1 mM for guanidine

KM=1.23 mM for procainamide

KM=0.07 mM for topotecan

KM=0.47 mM for estrone sulfate

KM=2.64 mM for acyclovir

KM=5.12 mM for ganciclovir

Vmax=1.185 nmol/min/mg enzyme toward TEA

Vmax=0.735 nmol/min/mg enzyme toward MPP

Vmax=0.135 nmol/min/mg enzyme toward cimetidine

Vmax=2.23 nmol/min/mg enzyme toward metformin

Vmax=0.89 nmol/min/mg enzyme toward guanidine

Vmax=3.78 nmol/min/mg enzyme toward procainamide

Vmax=0.21 nmol/min/mg enzyme toward topotecan

Vmax=0.265 nmol/min/mg enzyme toward estrone sulfate

Vmax=0.62 nmol/min/mg enzyme toward acyclovir

Vmax=1.08 nmol/min/mg enzyme toward ganciclovir

pH dependence:

Optimum pH is 8.5. Active from pH 6 to 8.5.

Sequence caution

The sequence AAH50592.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96FL8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96FL8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     286-290: ILGMV → LYEDG
     291-570: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q96FL8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     559-570: VGILVRFYVRIQ → AGVRWCDHSSLQPRTLGLQAILLCQPPE
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570Multidrug and toxin extrusion protein 1
PRO_0000312845

Regions

Topological domain1 – 3737Cytoplasmic Potential
Transmembrane38 – 5821Helical; Potential
Topological domain59 – 7214Extracellular Potential
Transmembrane73 – 9321Helical; Potential
Topological domain94 – 12330Cytoplasmic Potential
Transmembrane124 – 14421Helical; Potential
Topological domain145 – 1528Extracellular Potential
Transmembrane153 – 17321Helical; Potential
Topological domain174 – 1763Cytoplasmic Potential
Transmembrane177 – 19721Helical; Potential
Topological domain198 – 21619Extracellular Potential
Transmembrane217 – 23721Helical; Potential
Topological domain238 – 25619Cytoplasmic Potential
Transmembrane257 – 27620Helical; Potential
Topological domain277 – 29519Extracellular Potential
Transmembrane296 – 31621Helical; Potential
Topological domain317 – 33620Cytoplasmic Potential
Transmembrane337 – 35721Helical; Potential
Topological domain358 – 37013Extracellular Potential
Transmembrane371 – 39121Helical; Potential
Topological domain392 – 40817Cytoplasmic Potential
Transmembrane409 – 42921Helical; Potential
Topological domain430 – 4378Extracellular Potential
Transmembrane438 – 45821Helical; Potential
Topological domain459 – 54688Cytoplasmic Potential
Transmembrane547 – 56721Helical; Potential
Topological domain568 – 5703Extracellular Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.11

Natural variations

Alternative sequence286 – 2905ILGMV → LYEDG in isoform 2.
VSP_029903
Alternative sequence291 – 570280Missing in isoform 2.
VSP_029904
Alternative sequence559 – 57012VGILV…YVRIQ → AGVRWCDHSSLQPRTLGLQA ILLCQPPE in isoform 3.
VSP_029905
Natural variant3381V → I.
Corresponds to variant rs35790011 [ dbSNP | Ensembl ].
VAR_037587

Experimental info

Mutagenesis2731E → Q: No change in subcellular location and abolition of MATE1-dependent TEA transport activity. Ref.5
Sequence conflict501L → R in BAD96345. Ref.2
Sequence conflict3091P → L in BAA91852. Ref.1
Sequence conflict4481F → L in BAD96345. Ref.2
Sequence conflict5071N → D in BAD96345. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: CA940CBC2F6F5CC4

FASTA57061,922
        10         20         30         40         50         60 
MEAPEEPAPV RGGPEATLEV RGSRCLRLSA FREELRALLV LAGPAFLVQL MVFLISFISS 

        70         80         90        100        110        120 
VFCGHLGKLE LDAVTLAIAV INVTGVSVGF GLSSACDTLI SQTYGSQNLK HVGVILQRSA 

       130        140        150        160        170        180 
LVLLLCCFPC WALFLNTQHI LLLFRQDPDV SRLTQTYVTI FIPALPATFL YMLQVKYLLN 

       190        200        210        220        230        240 
QGIVLPQIVT GVAANLVNAL ANYLFLHQLH LGVIGSALAN LISQYTLALL LFLYILGKKL 

       250        260        270        280        290        300 
HQATWGGWSL ECLQDWASFL RLAIPSMLML CMEWWAYEVG SFLSGILGMV ELGAQSIVYE 

       310        320        330        340        350        360 
LAIIVYMVPA GFSVAASVRV GNALGAGDME QARKSSTVSL LITVLFAVAF SVLLLSCKDH 

       370        380        390        400        410        420 
VGYIFTTDRD IINLVAQVVP IYAVSHLFEA LACTSGGVLR GSGNQKVGAI VNTIGYYVVG 

       430        440        450        460        470        480 
LPIGIALMFA TTLGVMGLWS GIIICTVFQA VCFLGFIIQL NWKKACQQAQ VHANLKVNNV 

       490        500        510        520        530        540 
PRSGNSALPQ DPLHPGCPEN LEGILTNDVG KTGEPQSDQQ MRQEEPLPEH PQDGAKLSRK 

       550        560        570 
QLVLRRGLLL LGVFLILLVG ILVRFYVRIQ 

« Hide

Isoform 2 [UniParc].

Checksum: 662AE04699124D14
Show »

FASTA29031,907
Isoform 3 [UniParc].

Checksum: A1BF0F64D815E7A8
Show »

FASTA58663,549

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Cerebellum.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Colon and Eye.
[5]"A human transporter protein that mediates the final excretion step for toxic organic cations."
Otsuka M., Matsumoto T., Morimoto R., Arioka S., Omote H., Moriyama Y.
Proc. Natl. Acad. Sci. U.S.A. 102:17923-17928(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLU-273, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
[6]"Wide variety of locations for rodent MATE1, a transporter protein that mediates the final excretion step for toxic organic cations."
Hiasa M., Matsumoto T., Komatsu T., Moriyama Y.
Am. J. Physiol. 291:C678-C686(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"The MATE proteins as fundamental transporters of metabolic and xenobiotic organic cations."
Omote H., Hiasa M., Matsumoto T., Otsuka M., Moriyama Y.
Trends Pharmacol. Sci. 27:587-593(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Substrate specificity of MATE1 and MATE2-K, human multidrug and toxin extrusions/H(+)-organic cation antiporters."
Tanihara Y., Masuda S., Sato T., Katsura T., Ogawa O., Inui K.
Biochem. Pharmacol. 74:359-371(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[9]"Differential contribution of organic cation transporters, OCT2 and MATE1, in platinum agent-induced nephrotoxicity."
Yokoo S., Yonezawa A., Masuda S., Fukatsu A., Katsura T., Inui K.
Biochem. Pharmacol. 74:477-487(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Transport of paraquat by human organic cation transporters and multidrug and toxic compound extrusion family."
Chen Y., Zhang S., Sorani M., Giacomini K.M.
J. Pharmacol. Exp. Ther. 322:695-700(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK001709 mRNA. Translation: BAA91852.1.
AK222625 mRNA. Translation: BAD96345.1.
CH471212 Genomic DNA. Translation: EAW50893.1.
CH471212 Genomic DNA. Translation: EAW50894.1.
BC010661 mRNA. Translation: AAH10661.1.
BC050592 mRNA. Translation: AAH50592.1. Different initiation.
BC058882 mRNA. Translation: AAH58882.1.
CCDSCCDS11209.1. [Q96FL8-1]
RefSeqNP_060712.2. NM_018242.2. [Q96FL8-1]
UniGeneHs.232054.

3D structure databases

ProteinModelPortalQ96FL8.
SMRQ96FL8. Positions 28-454.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000270570.

Chemistry

ChEMBLCHEMBL1743126.
GuidetoPHARMACOLOGY1216.

Protein family/group databases

TCDB2.A.66.1.14. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

PTM databases

PhosphoSiteQ96FL8.

Polymorphism databases

DMDM74731723.

Proteomic databases

MaxQBQ96FL8.
PaxDbQ96FL8.
PRIDEQ96FL8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000270570; ENSP00000270570; ENSG00000142494. [Q96FL8-1]
ENST00000395585; ENSP00000378951; ENSG00000142494. [Q96FL8-3]
ENST00000457293; ENSP00000415586; ENSG00000142494. [Q96FL8-3]
GeneID55244.
KEGGhsa:55244.
UCSCuc002gvx.3. human. [Q96FL8-3]
uc002gvy.1. human. [Q96FL8-1]

Organism-specific databases

CTD55244.
GeneCardsGC17P019398.
H-InvDBHIX0013619.
HGNCHGNC:25588. SLC47A1.
HPAHPA021987.
MIM609832. gene.
neXtProtNX_Q96FL8.
PharmGKBPA162403808.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0534.
HOGENOMHOG000060313.
HOVERGENHBG056043.
KOK03327.
OMAFPRYLVM.
OrthoDBEOG7RRF71.
PhylomeDBQ96FL8.
TreeFamTF324441.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.
REACT_20633. Bile salt and organic anion SLC transporters.

Gene expression databases

ArrayExpressQ96FL8.
BgeeQ96FL8.
CleanExHS_SLC47A1.
GenevestigatorQ96FL8.

Family and domain databases

InterProIPR002528. MATE.
[Graphical view]
PfamPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsTIGR00797. matE. 1 hit.
ProtoNetSearch...

Other

GeneWikiSLC47A1.
GenomeRNAi55244.
NextBio59282.
PROQ96FL8.
SOURCESearch...

Entry information

Entry nameS47A1_HUMAN
AccessionPrimary (citable) accession number: Q96FL8
Secondary accession number(s): Q53HF5 expand/collapse secondary AC list , Q6PD77, Q86VL4, Q9NVA3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM