Q96FF9 (CDCA5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sororin

Alternative name(s):
Cell division cycle-associated protein 5
p35

Gene names

Name:

CDCA5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair. Ref.5 Ref.9 Ref.13
Subunit structure	Interacts with the APC/C complex By similarity. Interacts with the chromatin-bound cohesin complex; the interaction is indirect, occurs after DNA replication and requires acetylation of the cohesin component SMC3. Interacts (via the FGF motif) with PDS5A and PDS5B; the interaction is direct and prevents the interaction of PDS5A with WAPAL. Ref.5 Ref.9 Ref.13
Subcellular location	Nucleus. Chromosome. Cytoplasm. Note: Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown. Ref.5
Domain	The KEN box is required for the association with the APC/C complex By similarity.
Post-translational modification	Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5. Ref.5 Ubiquitinated by the APC/C complex in G1, leading to its degradation Probable. Ref.5
Miscellaneous	Named sororin after the Latin word 'soror', which means 'sister', because of its critical role in sister chromatid cohesion.
Sequence similarities	Belongs to the sororin family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Chromosome Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from direct assay Ref.5. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW double-strand break repair Inferred from mutant phenotype Ref.9. Source: UniProtKB mitotic chromosome condensation Inferred from mutant phenotype Ref.5. Source: UniProtKB mitotic metaphase plate congression Inferred from mutant phenotype Ref.5. Source: UniProtKB mitotic sister chromatid cohesion Inferred from mutant phenotype Ref.9Ref.13. Source: UniProtKB regulation of cohesin localization to chromatin Inferred from mutant phenotype Ref.9. Source: UniProtKB
Cellular_component	cohesin complex Inferred from electronic annotation. Source: Compara cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nuclear chromatin Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	chromatin binding Traceable author statement Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PDS5A	Q29RF7	2	EBI-718805,EBI-1175454

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Sororin		PRO_0000089449
Regions
	Motif	88 – 90	3	KEN box
	Motif	166 – 168	3	FGF motif
Amino acid modifications
	Modified residue	21	1	Phosphoserine Ref.14
	Modified residue	33	1	Phosphoserine Ref.11
	Modified residue	35	1	Phosphoserine Ref.11
	Modified residue	75	1	Phosphoserine Ref.7 Ref.10 Ref.14
	Modified residue	79	1	Phosphoserine Ref.14
	Modified residue	107	1	Phosphoserine Ref.12
	Modified residue	115	1	Phosphothreonine Ref.11
	Modified residue	209	1	Phosphoserine Ref.11 Ref.14 Ref.15
Natural variations
	Natural variant	156	1	S → Y. Corresponds to variant rs34020666 [ dbSNP | Ensembl ].		VAR_050777
Experimental info
	Mutagenesis	166 – 168	3	FGF → AGA: Alters interaction with PDS5A and PDS5B and the cohesin complex. Ref.13

Sequences

	Sequence		Length	Mass (Da)
	Q96FF9 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A4CD6768D3464040	FASTA	252	27,601
	10 20 30 40 50 60 MSGRRTRSGG AAQRSGPRAP SPTKPLRRSQ RKSGSELPSI LPEIWPKTPS AAAVRKPIVL 70 80 90 100 110 120 KRIVAHAVEV PAVQSPRRSP RISFFLEKEN EPPGRELTKE DLFKTHSVPA TPTSTPVPNP 130 140 150 160 170 180 EAESSSKEGE LDARDLEMSK KVRRSYSRLE TLGSASTSTP GRRSCFGFEG LLGAEDLSGV 190 200 210 220 230 240 SPVVCSKLTE VPRVCAKPWA PDMTLPGISP PPEKQKRKKK KMPEILKTEL DEWAAAMNAE 250 FEAAEQFDLL VE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drug target discovery by gene expression analysis: cell cycle genes." Walker M.G. Curr. Cancer Drug Targets 1:73-83(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	"Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates." Rankin S., Ayad N.G., Kirschner M.W. Mol. Cell 18:185-200(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, UBIQUITINATION, IDENTIFICATION IN A COMPLEX WITH SMC1A; SMC3; RAD21; PDS5A AND PDS5B.
[6]	Erratum Rankin S., Ayad N.G., Kirschner M.W. Mol. Cell 18:609-609(2005)
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Sororin is required for stable binding of cohesin to chromatin and for sister chromatid cohesion in interphase." Schmitz J., Watrin E., Lenart P., Mechtler K., Peters J.M. Curr. Biol. 17:630-636(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE COHESIN COMPLEX.
[10]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33; SER-35; THR-115 AND SER-209, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Sororin mediates sister chromatid cohesion by antagonizing wapl." Nishiyama T., Ladurner R., Schmitz J., Kreidl E., Schleiffer A., Bhaskara V., Bando M., Shirahige K., Hyman A.A., Mechtler K., Peters J.M. Cell 143:737-749(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PDS5A AND PDS5B, MUTAGENESIS OF 166-PHE--PHE-168, FGF MOTIF.
[14]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-75; SER-79 AND SER-209, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-209, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BG354578 mRNA. No translation available. AK291490 mRNA. Translation: BAF84179.1. CH471076 Genomic DNA. Translation: EAW74342.1. BC011000 mRNA. Translation: AAH11000.1.
IPI	IPI00061989.
RefSeq	NP_542399.1. NM_080668.3.
UniGene	Hs.434886.
3D structure databases
ProteinModelPortal	Q96FF9.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47377N.
IntAct	Q96FF9. 9 interactions.
MINT	MINT-1416799.
STRING	9606.ENSP00000275517.
PTM databases
PhosphoSite	Q96FF9.
Polymorphism databases
DMDM	68565257.
Proteomic databases
PaxDb	Q96FF9.
PeptideAtlas	Q96FF9.
PRIDE	Q96FF9.
Protocols and materials databases
DNASU	113130.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000275517; ENSP00000275517; ENSG00000146670.
GeneID	113130.
KEGG	hsa:113130.
UCSC	uc001ocp.2. human.
Organism-specific databases
CTD	113130.
GeneCards	GC11M064833.
H-InvDB	HIX0009780.
HGNC	HGNC:14626. CDCA5.
HPA	HPA023691.
MIM	609374. gene.
neXtProt	NX_Q96FF9.
PharmGKB	PA26278.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG43755.
HOGENOM	HOG000232187.
HOVERGEN	HBG080614.
OrthoDB	EOG4MPHR8.
PhylomeDB	Q96FF9.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle. REACT_21300. Mitotic M-M/G1 phases.
Gene expression databases
ArrayExpress	Q96FF9.
Bgee	Q96FF9.
CleanEx	HS_CDCA5.
Genevestigator	Q96FF9.
GermOnline	ENSG00000146670. Homo sapiens.
Family and domain databases
InterPro	IPR018605. Sororin. [Graphical view]
Pfam	PF09666. Sororin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CDCA5. human.
GenomeRNAi	113130.
NextBio	78748.
SOURCE	Search...

Entry information

Entry name

CDCA5_HUMAN

Accession

Primary (citable) accession number: Q96FF9
Secondary accession number(s): A8K625

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.