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Q96FF9

- CDCA5_HUMAN

UniProt

Q96FF9 - CDCA5_HUMAN

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Protein
Sororin
Gene
CDCA5
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.3 Publications

GO - Molecular functioni

  1. chromatin binding Source: UniProtKB
  2. protein binding Source: UniProtKB

GO - Biological processi

  1. G1/S transition of mitotic cell cycle Source: UniProtKB
  2. double-strand break repair Source: UniProtKB
  3. mitotic cell cycle Source: Reactome
  4. mitotic chromosome condensation Source: UniProtKB
  5. mitotic interphase Source: UniProtKB
  6. mitotic metaphase plate congression Source: UniProtKB
  7. mitotic nuclear division Source: UniProtKB
  8. mitotic sister chromatid cohesion Source: UniProtKB
  9. regulation of cohesin localization to chromatin Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_150266. Establishment of Sister Chromatid Cohesion.
REACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.

Names & Taxonomyi

Protein namesi
Recommended name:
Sororin
Alternative name(s):
Cell division cycle-associated protein 5
p35
Gene namesi
Name:CDCA5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:14626. CDCA5.

Subcellular locationi

Nucleus. Chromosome. Cytoplasm
Note: Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown.1 Publication

GO - Cellular componenti

  1. chromosome Source: Reactome
  2. chromosome, centromeric region Source: Reactome
  3. cohesin complex Source: Ensembl
  4. cytoplasm Source: UniProtKB
  5. cytosol Source: Reactome
  6. nuclear chromatin Source: UniProtKB
  7. nucleoplasm Source: Reactome
  8. nucleus Source: UniProtKB
  9. plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi166 – 1683FGF → AGA: Alters interaction with PDS5A and PDS5B and the cohesin complex. 1 Publication

Organism-specific databases

PharmGKBiPA26278.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252Sororin
PRO_0000089449Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211Phosphoserine1 Publication
Modified residuei33 – 331Phosphoserine1 Publication
Modified residuei35 – 351Phosphoserine1 Publication
Modified residuei75 – 751Phosphoserine3 Publications
Modified residuei79 – 791Phosphoserine1 Publication
Modified residuei107 – 1071Phosphoserine1 Publication
Modified residuei115 – 1151Phosphothreonine1 Publication
Modified residuei209 – 2091Phosphoserine3 Publications

Post-translational modificationi

Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.1 Publication
Ubiquitinated by the APC/C complex in G1, leading to its degradation Inferred.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ96FF9.
PaxDbiQ96FF9.
PeptideAtlasiQ96FF9.
PRIDEiQ96FF9.

PTM databases

PhosphoSiteiQ96FF9.

Expressioni

Gene expression databases

ArrayExpressiQ96FF9.
BgeeiQ96FF9.
CleanExiHS_CDCA5.
GenevestigatoriQ96FF9.

Organism-specific databases

HPAiHPA023691.

Interactioni

Subunit structurei

Interacts with the APC/C complex By similarity. Interacts with the chromatin-bound cohesin complex; the interaction is indirect, occurs after DNA replication and requires acetylation of the cohesin component SMC3. Interacts (via the FGF motif) with PDS5A and PDS5B; the interaction is direct and prevents the interaction of PDS5A with WAPAL.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PDS5AQ29RF72EBI-718805,EBI-1175454

Protein-protein interaction databases

BioGridi125225. 19 interactions.
DIPiDIP-47377N.
IntActiQ96FF9. 10 interactions.
MINTiMINT-1416799.
STRINGi9606.ENSP00000275517.

Structurei

3D structure databases

ProteinModelPortaliQ96FF9.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi88 – 903KEN box
Motifi166 – 1683FGF motif

Domaini

The KEN box is required for the association with the APC/C complex By similarity.

Sequence similaritiesi

Belongs to the sororin family.

Phylogenomic databases

eggNOGiNOG43755.
HOGENOMiHOG000232187.
HOVERGENiHBG080614.
KOiK17390.
PhylomeDBiQ96FF9.
TreeFamiTF101070.

Family and domain databases

InterProiIPR018605. Sororin.
[Graphical view]
PfamiPF09666. Sororin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96FF9-1 [UniParc]FASTAAdd to Basket

« Hide

MSGRRTRSGG AAQRSGPRAP SPTKPLRRSQ RKSGSELPSI LPEIWPKTPS    50
AAAVRKPIVL KRIVAHAVEV PAVQSPRRSP RISFFLEKEN EPPGRELTKE 100
DLFKTHSVPA TPTSTPVPNP EAESSSKEGE LDARDLEMSK KVRRSYSRLE 150
TLGSASTSTP GRRSCFGFEG LLGAEDLSGV SPVVCSKLTE VPRVCAKPWA 200
PDMTLPGISP PPEKQKRKKK KMPEILKTEL DEWAAAMNAE FEAAEQFDLL 250
VE 252
Length:252
Mass (Da):27,601
Last modified:December 1, 2001 - v1
Checksum:iA4CD6768D3464040
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti156 – 1561S → Y.
Corresponds to variant rs34020666 [ dbSNP | Ensembl ].
VAR_050777

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BG354578 mRNA. No translation available.
AK291490 mRNA. Translation: BAF84179.1.
CH471076 Genomic DNA. Translation: EAW74342.1.
BC011000 mRNA. Translation: AAH11000.1.
CCDSiCCDS8091.1.
RefSeqiNP_542399.1. NM_080668.3.
UniGeneiHs.434886.

Genome annotation databases

EnsembliENST00000275517; ENSP00000275517; ENSG00000146670.
GeneIDi113130.
KEGGihsa:113130.
UCSCiuc001ocp.2. human.

Polymorphism databases

DMDMi68565257.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BG354578 mRNA. No translation available.
AK291490 mRNA. Translation: BAF84179.1 .
CH471076 Genomic DNA. Translation: EAW74342.1 .
BC011000 mRNA. Translation: AAH11000.1 .
CCDSi CCDS8091.1.
RefSeqi NP_542399.1. NM_080668.3.
UniGenei Hs.434886.

3D structure databases

ProteinModelPortali Q96FF9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 125225. 19 interactions.
DIPi DIP-47377N.
IntActi Q96FF9. 10 interactions.
MINTi MINT-1416799.
STRINGi 9606.ENSP00000275517.

PTM databases

PhosphoSitei Q96FF9.

Polymorphism databases

DMDMi 68565257.

Proteomic databases

MaxQBi Q96FF9.
PaxDbi Q96FF9.
PeptideAtlasi Q96FF9.
PRIDEi Q96FF9.

Protocols and materials databases

DNASUi 113130.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000275517 ; ENSP00000275517 ; ENSG00000146670 .
GeneIDi 113130.
KEGGi hsa:113130.
UCSCi uc001ocp.2. human.

Organism-specific databases

CTDi 113130.
GeneCardsi GC11M064833.
H-InvDB HIX0009780.
HGNCi HGNC:14626. CDCA5.
HPAi HPA023691.
MIMi 609374. gene.
neXtProti NX_Q96FF9.
PharmGKBi PA26278.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG43755.
HOGENOMi HOG000232187.
HOVERGENi HBG080614.
KOi K17390.
PhylomeDBi Q96FF9.
TreeFami TF101070.

Enzyme and pathway databases

Reactomei REACT_150266. Establishment of Sister Chromatid Cohesion.
REACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.

Miscellaneous databases

ChiTaRSi CDCA5. human.
GeneWikii CDCA5.
GenomeRNAii 113130.
NextBioi 78748.
PROi Q96FF9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q96FF9.
Bgeei Q96FF9.
CleanExi HS_CDCA5.
Genevestigatori Q96FF9.

Family and domain databases

InterProi IPR018605. Sororin.
[Graphical view ]
Pfami PF09666. Sororin. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Drug target discovery by gene expression analysis: cell cycle genes."
    Walker M.G.
    Curr. Cancer Drug Targets 1:73-83(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  5. "Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates."
    Rankin S., Ayad N.G., Kirschner M.W.
    Mol. Cell 18:185-200(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, UBIQUITINATION, IDENTIFICATION IN A COMPLEX WITH SMC1A; SMC3; RAD21; PDS5A AND PDS5B.
  6. Erratum
    Rankin S., Ayad N.G., Kirschner M.W.
    Mol. Cell 18:609-609(2005)
  7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Sororin is required for stable binding of cohesin to chromatin and for sister chromatid cohesion in interphase."
    Schmitz J., Watrin E., Lenart P., Mechtler K., Peters J.M.
    Curr. Biol. 17:630-636(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE COHESIN COMPLEX.
  10. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33; SER-35; THR-115 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  14. Cited for: FUNCTION, INTERACTION WITH PDS5A AND PDS5B, MUTAGENESIS OF 166-PHE--PHE-168, FGF MOTIF.
  15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-75; SER-79 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  16. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCDCA5_HUMAN
AccessioniPrimary (citable) accession number: Q96FF9
Secondary accession number(s): A8K625
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Named sororin after the Latin word 'soror', which means 'sister', because of its critical role in sister chromatid cohesion.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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