Q96F25 (ALG14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine transferase subunit ALG14 homolog

Gene names

Name:

ALG14

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in protein N-glycosylation. May play a role in the second step of the dolichol-linked oligosaccharide pathway. May anchor the catalytic subunit ALG13 to the ER. Ref.4
Subunit structure	Heterodimer with ALG13 isoform 2 to form a functional enzyme By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Probable.
Sequence similarities	Belongs to the ALG14 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dolichol-linked oligosaccharide biosynthetic process Traceable author statement. Source: Reactome post-translational protein modification Traceable author statement. Source: Reactome protein N-linked glycosylation via asparagine Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	UDP-N-acetylglucosamine transferase subunit ALG14 homolog		PRO_0000265116
Regions
	Topological domain	1 – 3	3	Lumenal Potential
	Transmembrane	4 – 24	21	Helical; Potential
	Topological domain	25 – 216	192	Cytoplasmic Potential
Natural variations
	Natural variant	14	1	V → M. Corresponds to variant rs11165298 [ dbSNP | Ensembl ].		VAR_029635

Sequences

	Sequence		Length	Mass (Da)
	Q96F25 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 0724FEAE33A841E8	FASTA	216	24,151
	10 20 30 40 50 60 MVCVLVLAAA AGAVAVFLIL RIWVVLRSMD VTPRESLSIL VVAGSGGHTT EILRLLGSLS 70 80 90 100 110 120 NAYSPRHYVI ADTDEMSANK INSFELDRAD RDPSNMYTKY YIHRIPRSRE VQQSWPSTVF 130 140 150 160 170 180 TTLHSMWLSF PLIHRVKPDL VLCNGPGTCV PICVSALLLG ILGIKKVIIV YVESICRVET 190 200 210 LSMSGKILFH LSDYFIVQWP ALKEKYPKSV YLGRIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[4]	"Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic reticulum to form a novel bipartite UDP-N-acetylglucosamine transferase required for the second step of N-linked glycosylation." Gao X.-D., Tachikawa H., Sato T., Jigami Y., Dean N. J. Biol. Chem. 280:36254-36262(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK289395 mRNA. Translation: BAF82084.1. CH471097 Genomic DNA. Translation: EAW73027.1. BC011706 mRNA. Translation: AAH11706.1.
IPI	IPI00061677.
RefSeq	NP_659425.1. NM_144988.3.
UniGene	Hs.408927.
3D structure databases
ProteinModelPortal	Q96F25.
SMR	Q96F25. Positions 37-71.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000359224.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
PTM databases
PhosphoSite	Q96F25.
Polymorphism databases
DMDM	74731649.
Proteomic databases
PaxDb	Q96F25.
PRIDE	Q96F25.
Protocols and materials databases
DNASU	199857.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370205; ENSP00000359224; ENSG00000172339.
GeneID	199857.
KEGG	hsa:199857.
UCSC	uc001dra.2. human.
Organism-specific databases
CTD	199857.
GeneCards	GC01M095449.
HGNC	HGNC:28287. ALG14.
HPA	HPA031829.
MIM	612866. gene.
neXtProt	NX_Q96F25.
PharmGKB	PA142672628.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0707.
HOGENOM	HOG000182099.
HOVERGEN	HBG059605.
InParanoid	Q96F25.
KO	K07441.
OMA	YTKYYIH.
OrthoDB	EOG48D0WS.
PhylomeDB	Q96F25.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
Gene expression databases
Bgee	Q96F25.
CleanEx	HS_ALG14.
Genevestigator	Q96F25.
GermOnline	ENSG00000172339. Homo sapiens.
Family and domain databases
InterPro	IPR013969. Oligosacch_biosynth_Alg14. [Graphical view]
PANTHER	PTHR12154. PTHR12154. 1 hit.
Pfam	PF08660. Alg14. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ALG14. human.
GenomeRNAi	199857.
NextBio	89753.
SOURCE	Search...

Entry information

Entry name

ALG14_HUMAN

Accession

Primary (citable) accession number: Q96F25
Secondary accession number(s): A8K030

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.