Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diamine acetyltransferase 2

Gene

SAT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine > spermidine = spermine >> N1acetylspermine = putrescine.

Catalytic activityi

Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.

Pathwayi: putrescine degradation

This protein is involved in step 1 of the subpathway that synthesizes N-acetylputrescine from putrescine.
Proteins known to be involved in this subpathway in this organism are:
  1. Diamine acetyltransferase 2 (SAT2), Diamine acetyltransferase 1 (SAT1)
This subpathway is part of the pathway putrescine degradation, which is itself part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetylputrescine from putrescine, the pathway putrescine degradation and in Amine and polyamine degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92Substrate; via carbonyl oxygenBy similarity1
Binding sitei140Acetyl-CoA1 Publication1
Binding sitei152SubstrateBy similarity1

GO - Molecular functioni

  • diamine N-acetyltransferase activity Source: UniProtKB
  • spermidine binding Source: GO_Central

GO - Biological processi

  • nor-spermidine metabolic process Source: UniProtKB
  • putrescine acetylation Source: UniProtKB
  • putrescine catabolic process Source: UniProtKB-UniPathway
  • spermidine acetylation Source: UniProtKB
  • spermine acetylation Source: UniProtKB
  • spermine catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciZFISH:ENSG00000158480-MONOMER.
ZFISH:HS06837-MONOMER.
BRENDAi2.3.1.57. 2681.
UniPathwayiUPA00188; UER00363.

Names & Taxonomyi

Protein namesi
Recommended name:
Diamine acetyltransferase 2 (EC:2.3.1.57)
Alternative name(s):
Polyamine N-acetyltransferase 2
Spermidine/spermine N(1)-acetyltransferase 2
Thialysine N-epsilon-acetyltransferase
Gene namesi
Name:SAT2
Synonyms:SSAT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:23160. SAT2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi112483.
OpenTargetsiENSG00000141504.
PharmGKBiPA134979941.

Chemistry databases

ChEMBLiCHEMBL3509592.
DrugBankiDB00127. Spermine.

Polymorphism and mutation databases

BioMutaiSAT2.
DMDMi51339204.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745981 – 170Diamine acetyltransferase 2Add BLAST170

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ96F10.
MaxQBiQ96F10.
PaxDbiQ96F10.
PeptideAtlasiQ96F10.
PRIDEiQ96F10.

PTM databases

iPTMnetiQ96F10.
PhosphoSitePlusiQ96F10.

Expressioni

Tissue specificityi

Widely expressed.

Inductioni

Not inducible by polyamine analogs.

Gene expression databases

BgeeiENSG00000141504.
CleanExiHS_SAT2.
ExpressionAtlasiQ96F10. baseline and differential.
GenevisibleiQ96F10. HS.

Organism-specific databases

HPAiHPA022136.
HPA057096.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-748746,EBI-748746
SAT1P216733EBI-748746,EBI-711613

Protein-protein interaction databases

BioGridi125190. 7 interactors.
IntActiQ96F10. 1 interactor.
MINTiMINT-1476751.
STRINGi9606.ENSP00000269298.

Structurei

Secondary structure

1170
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi11 – 13Combined sources3
Helixi14 – 28Combined sources15
Helixi31 – 33Combined sources3
Helixi38 – 46Combined sources9
Beta strandi47 – 49Combined sources3
Beta strandi53 – 59Combined sources7
Beta strandi70 – 82Combined sources13
Turni83 – 85Combined sources3
Beta strandi86 – 96Combined sources11
Helixi98 – 100Combined sources3
Beta strandi102 – 104Combined sources3
Helixi105 – 119Combined sources15
Beta strandi124 – 130Combined sources7
Helixi134 – 142Combined sources9
Helixi148 – 152Combined sources5
Beta strandi154 – 160Combined sources7
Helixi162 – 167Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BEIX-ray1.84A/B2-170[»]
2Q4VX-ray1.84A/B2-170[»]
ProteinModelPortaliQ96F10.
SMRiQ96F10.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96F10.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 168N-acetyltransferasePROSITE-ProRule annotationAdd BLAST165

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 28Substrate bindingBy similarity2
Regioni94 – 96Acetyl-CoA binding3
Regioni102 – 107Acetyl-CoA binding6
Regioni133 – 135Acetyl-CoA binding3

Sequence similaritiesi

Belongs to the acetyltransferase family.Curated
Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IU7S. Eukaryota.
ENOG4111PFJ. LUCA.
GeneTreeiENSGT00440000039972.
HOGENOMiHOG000078521.
HOVERGENiHBG063175.
InParanoidiQ96F10.
KOiK00657.
OMAiRICVERG.
OrthoDBiEOG091G0UNU.
PhylomeDBiQ96F10.
TreeFamiTF319736.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
IPR032959. SAT2.
[Graphical view]
PANTHERiPTHR10545:SF41. PTHR10545:SF41. 1 hit.
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96F10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVRIREAK EGDCGDILRL IRELAEFEKL SDQVKISEEA LRADGFGDNP
60 70 80 90 100
FYHCLVAEIL PAPGKLLGPC VVGYGIYYFI YSTWKGRTIY LEDIYVMPEY
110 120 130 140 150
RGQGIGSKII KKVAEVALDK GCSQFRLAVL DWNQRAMDLY KALGAQDLTE
160 170
AEGWHFFCFQ GEATRKLAGK
Length:170
Mass (Da):19,155
Last modified:December 1, 2001 - v1
Checksum:i68D46F0E28186C68
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020465126R → C.Corresponds to variant rs13894dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF348524 mRNA. Translation: AAL83905.1.
BC011751 mRNA. Translation: AAH11751.1.
CCDSiCCDS11116.1.
RefSeqiNP_001307775.1. NM_001320846.1.
NP_001307776.1. NM_001320847.1.
NP_597998.1. NM_133491.4.
UniGeneiHs.10846.

Genome annotation databases

EnsembliENST00000269298; ENSP00000269298; ENSG00000141504.
GeneIDi112483.
KEGGihsa:112483.
UCSCiuc002gic.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF348524 mRNA. Translation: AAL83905.1.
BC011751 mRNA. Translation: AAH11751.1.
CCDSiCCDS11116.1.
RefSeqiNP_001307775.1. NM_001320846.1.
NP_001307776.1. NM_001320847.1.
NP_597998.1. NM_133491.4.
UniGeneiHs.10846.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BEIX-ray1.84A/B2-170[»]
2Q4VX-ray1.84A/B2-170[»]
ProteinModelPortaliQ96F10.
SMRiQ96F10.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125190. 7 interactors.
IntActiQ96F10. 1 interactor.
MINTiMINT-1476751.
STRINGi9606.ENSP00000269298.

Chemistry databases

ChEMBLiCHEMBL3509592.
DrugBankiDB00127. Spermine.

PTM databases

iPTMnetiQ96F10.
PhosphoSitePlusiQ96F10.

Polymorphism and mutation databases

BioMutaiSAT2.
DMDMi51339204.

Proteomic databases

EPDiQ96F10.
MaxQBiQ96F10.
PaxDbiQ96F10.
PeptideAtlasiQ96F10.
PRIDEiQ96F10.

Protocols and materials databases

DNASUi112483.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000269298; ENSP00000269298; ENSG00000141504.
GeneIDi112483.
KEGGihsa:112483.
UCSCiuc002gic.3. human.

Organism-specific databases

CTDi112483.
DisGeNETi112483.
GeneCardsiSAT2.
HGNCiHGNC:23160. SAT2.
HPAiHPA022136.
HPA057096.
MIMi611463. gene.
neXtProtiNX_Q96F10.
OpenTargetsiENSG00000141504.
PharmGKBiPA134979941.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IU7S. Eukaryota.
ENOG4111PFJ. LUCA.
GeneTreeiENSGT00440000039972.
HOGENOMiHOG000078521.
HOVERGENiHBG063175.
InParanoidiQ96F10.
KOiK00657.
OMAiRICVERG.
OrthoDBiEOG091G0UNU.
PhylomeDBiQ96F10.
TreeFamiTF319736.

Enzyme and pathway databases

UniPathwayiUPA00188; UER00363.
BioCyciZFISH:ENSG00000158480-MONOMER.
ZFISH:HS06837-MONOMER.
BRENDAi2.3.1.57. 2681.

Miscellaneous databases

EvolutionaryTraceiQ96F10.
GeneWikiiSAT2.
GenomeRNAii112483.
PROiQ96F10.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141504.
CleanExiHS_SAT2.
ExpressionAtlasiQ96F10. baseline and differential.
GenevisibleiQ96F10. HS.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
IPR032959. SAT2.
[Graphical view]
PANTHERiPTHR10545:SF41. PTHR10545:SF41. 1 hit.
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAT2_HUMAN
AccessioniPrimary (citable) accession number: Q96F10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Under physiological conditions, SSAT2 is expressed at lower level that SSAT1 (SSAT). Many tissues express only SSAT1, several tissues express both SSAT1 and SSAT2, and bone, cervix, ovary and pineal gland expressed only SSAT2.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.