Q96F07 (CYFP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytoplasmic FMR1-interacting protein 2

Alternative name(s):
p53-inducible protein 121

Gene names

Name:	CYFIP2
Synonyms:	KIAA1168, PIR121

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1278 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in T-cell adhesion and p53/TP53-dependent induction of apoptosis. Does not bind RNA. Ref.1 Ref.9 Ref.11
Subunit structure	Interacts with FMR1, FXR1 AND FXR2. Component of the WAVE1 complex composed of ABI2, CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Ref.9 UniProtKB Q5SQX6 Ref.3
Subcellular location	Cytoplasm. Cytoplasm › perinuclear region. Cell junction › synapse › synaptosome. Note: Highly expressed in the perinuclear region. Enriched in synaptosomes. Treatment with leptomycin-B triggers translocation to the nucleus. Ref.1 Ref.11
Tissue specificity	Expressed in T-cells. Increased expression is observed in CD4+ T-lymphocytes from patients with multiple sclerosis (at protein level). Ref.9
Induction	By p53/TP53. Ref.11
Sequence similarities	Belongs to the CYFIP family.
RNA editing	Edited at position 320. Partially edited. Editing appears to be brain-specific. Ref.2 Ref.4 Ref.10
Sequence caution	The sequence BAA86482.2 differs from that shown. Reason: Erroneous initiation. The sequence BAD97324.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Apoptosis Cell adhesion
Cellular component	Cell junction Cytoplasm Synapse Synaptosome
Coding sequence diversity	Alternative splicing RNA editing
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of cysteine-type endopeptidase activity Inferred from direct assay PubMed 18387192. Source: BHF-UCL apoptotic process Inferred from direct assay Ref.1. Source: UniProtKB cell-cell adhesion Inferred from direct assay Ref.9. Source: UniProtKB positive regulation of proteolysis Inferred from direct assay PubMed 18387192. Source: BHF-UCL
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB synapse Inferred from direct assay PubMed 11438699. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FMR1	Q06787	2	EBI-2433893,EBI-366305

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.3 (identifier: Q96F07-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: Q96F07-2)

The sequence of this isoform differs from the canonical sequence as follows:
     558-582: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1278	1278	Cytoplasmic FMR1-interacting protein 2		PRO_0000279709
Amino acid modifications
	Modified residue	108	1	Phosphotyrosine By similarity
	Modified residue	1062	1	N6-acetyllysine Ref.12
Natural variations
	Alternative sequence	558 – 582	25	Missing in isoform 2. Ref.1		VSP_052349
	Natural variant	320	1	K → E in RNA edited version. Ref.10 Corresponds to variant rs3207362 [ dbSNP | Ensembl ].		VAR_030953
Experimental info
	Sequence conflict	796	1	I → T in AAH11762. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 6, 2007. Version 2. Checksum: D6562B1204DC2463	FASTA	1,278	148,399
	10 20 30 40 50 60 MTTHVTLEDA LSNVDLLEEL PLPDQQPCIE PPPSSIMYQA NFDTNFEDRN AFVTGIARYI 70 80 90 100 110 120 EQATVHSSMN EMLEEGHEYA VMLYTWRSCS RAIPQVKCNE QPNRVEIYEK TVEVLEPEVT 130 140 150 160 170 180 KLMKFMYFQR KAIERFCSEV KRLCHAERRK DFVSEAYLLT LGKFINMFAV LDELKNMKCS 190 200 210 220 230 240 VKNDHSAYKR AAQFLRKMAD PQSIQESQNL SMFLANHNRI TQCLHQQLEV IPGYEELLAD 250 260 270 280 290 300 IVNICVDYYE NKMYLTPSEK HMLLKVMGFG LYLMDGNVSN IYKLDAKKRI NLSKIDKFFK 310 320 330 340 350 360 QLQVVPLFGD MQIELARYIK TSAHYEENKS KWTCTQSSIS PQYNICEQMV QIRDDHIRFI 370 380 390 400 410 420 SELARYSNSE VVTGSGLDSQ KSDEEYRELF DLALRGLQLL SKWSAHVMEV YSWKLVHPTD 430 440 450 460 470 480 KFCNKDCPGT AEEYERATRY NYTSEEKFAF VEVIAMIKGL QVLMGRMESV FNQAIRNTIY 490 500 510 520 530 540 AALQDFAQVT LREPLRQAVR KKKNVLISVL QAIRKTICDW EGGREPPNDP CLRGEKDPKG 550 560 570 580 590 600 GFDIKVPRRA VGPSSTQACQ WSPRALFHPT GGTQGRRGCR SLLYMVRTML ESLIADKSGS 610 620 630 640 650 660 KKTLRSSLDG PIVLAIEDFH KQSFFFTHLL NISEALQQCC DLSQLWFREF FLELTMGRRI 670 680 690 700 710 720 QFPIEMSMPW ILTDHILETK EPSMMEYVLY PLDLYNDSAY YALTKFKKQF LYDEIEAEVN 730 740 750 760 770 780 LCFDQFVYKL ADQIFAYYKA MAGSVLLDKR FRAECKNYGV IIPYPPSNRY ETLLKQRHVQ 790 800 810 820 830 840 LLGRSIDLNR LITQRISAAM YKSLDQAISR FESEDLTSIV ELEWLLEINR LTHRLLCKHM 850 860 870 880 890 900 TLDSFDAMFR EANHNVSAPY GRITLHVFWE LNFDFLPNYC YNGSTNRFVR TAIPFTQEPQ 910 920 930 940 950 960 RDKPANVQPY YLYGSKPLNI AYSHIYSSYR NFVGPPHFKT ICRLLGYQGI AVVMEELLKI 970 980 990 1000 1010 1020 VKSLLQGTIL QYVKTLIEVM PKICRLPRHE YGSPGILEFF HHQLKDIIEY AELKTDVFQS 1030 1040 1050 1060 1070 1080 LREVGNAILF CLLIEQALSQ EEVCDLLHAA PFQNILPRVY IKEGERLEVR MKRLEAKYAP 1090 1100 1110 1120 1130 1140 LHLVPLIERL GTPQQIAIAR EGDLLTKERL CCGLSMFEVI LTRIRSYLQD PIWRGPPPTN 1150 1160 1170 1180 1190 1200 GVMHVDECVE FHRLWSAMQF VYCIPVGTNE FTAEQCFGDG LNWAGCSIIV LLGQQRRFDL 1210 1220 1230 1240 1250 1260 FDFCYHLLKV QRQDGKDEII KNVPLKKMAD RIRKYQILNN EVFAILNKYM KSVETDSSTV 1270 EHVRCFQPPI HQSLATTC
	Isoform 2 [UniParc]. Checksum: 3D6B1D906991A24F	FASTA	1,253	145,673
	10 20 30 40 50 60 MTTHVTLEDA LSNVDLLEEL PLPDQQPCIE PPPSSIMYQA NFDTNFEDRN AFVTGIARYI 70 80 90 100 110 120 EQATVHSSMN EMLEEGHEYA VMLYTWRSCS RAIPQVKCNE QPNRVEIYEK TVEVLEPEVT 130 140 150 160 170 180 KLMKFMYFQR KAIERFCSEV KRLCHAERRK DFVSEAYLLT LGKFINMFAV LDELKNMKCS 190 200 210 220 230 240 VKNDHSAYKR AAQFLRKMAD PQSIQESQNL SMFLANHNRI TQCLHQQLEV IPGYEELLAD 250 260 270 280 290 300 IVNICVDYYE NKMYLTPSEK HMLLKVMGFG LYLMDGNVSN IYKLDAKKRI NLSKIDKFFK 310 320 330 340 350 360 QLQVVPLFGD MQIELARYIK TSAHYEENKS KWTCTQSSIS PQYNICEQMV QIRDDHIRFI 370 380 390 400 410 420 SELARYSNSE VVTGSGLDSQ KSDEEYRELF DLALRGLQLL SKWSAHVMEV YSWKLVHPTD 430 440 450 460 470 480 KFCNKDCPGT AEEYERATRY NYTSEEKFAF VEVIAMIKGL QVLMGRMESV FNQAIRNTIY 490 500 510 520 530 540 AALQDFAQVT LREPLRQAVR KKKNVLISVL QAIRKTICDW EGGREPPNDP CLRGEKDPKG 550 560 570 580 590 600 GFDIKVPRRA VGPSSTQLYM VRTMLESLIA DKSGSKKTLR SSLDGPIVLA IEDFHKQSFF 610 620 630 640 650 660 FTHLLNISEA LQQCCDLSQL WFREFFLELT MGRRIQFPIE MSMPWILTDH ILETKEPSMM 670 680 690 700 710 720 EYVLYPLDLY NDSAYYALTK FKKQFLYDEI EAEVNLCFDQ FVYKLADQIF AYYKAMAGSV 730 740 750 760 770 780 LLDKRFRAEC KNYGVIIPYP PSNRYETLLK QRHVQLLGRS IDLNRLITQR ISAAMYKSLD 790 800 810 820 830 840 QAISRFESED LTSIVELEWL LEINRLTHRL LCKHMTLDSF DAMFREANHN VSAPYGRITL 850 860 870 880 890 900 HVFWELNFDF LPNYCYNGST NRFVRTAIPF TQEPQRDKPA NVQPYYLYGS KPLNIAYSHI 910 920 930 940 950 960 YSSYRNFVGP PHFKTICRLL GYQGIAVVME ELLKIVKSLL QGTILQYVKT LIEVMPKICR 970 980 990 1000 1010 1020 LPRHEYGSPG ILEFFHHQLK DIIEYAELKT DVFQSLREVG NAILFCLLIE QALSQEEVCD 1030 1040 1050 1060 1070 1080 LLHAAPFQNI LPRVYIKEGE RLEVRMKRLE AKYAPLHLVP LIERLGTPQQ IAIAREGDLL 1090 1100 1110 1120 1130 1140 TKERLCCGLS MFEVILTRIR SYLQDPIWRG PPPTNGVMHV DECVEFHRLW SAMQFVYCIP 1150 1160 1170 1180 1190 1200 VGTNEFTAEQ CFGDGLNWAG CSIIVLLGQQ RRFDLFDFCY HLLKVQRQDG KDEIIKNVPL 1210 1220 1230 1240 1250 KKMADRIRKY QILNNEVFAI LNKYMKSVET DSSTVEHVRC FQPPIHQSLA TTC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Increased apoptosis induction by 121F mutant p53." Saller E., Tom E., Brunori M., Otter M., Estreicher A., Mack D.H., Iggo R. EMBO J. 18:4424-4437(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION.
[2]	"Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain." Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O. DNA Res. 6:329-336(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), RNA EDITING OF POSITION 320. Tissue: Brain.
[3]	Ohara O., Nagase T., Kikuno R. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), RNA EDITING OF POSITION 320. Tissue: Amygdala.
[5]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[6]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lymph.
[9]	"CYFIP2 is highly abundant in CD4+ cells from multiple sclerosis patients and is involved in T cell adhesion." Mayne M., Moffatt T., Kong H., McLaren P.J., Fowke K.R., Becker K.G., Namaka M., Schenck A., Bardoni B., Bernstein C.N., Melanson M. Eur. J. Immunol. 34:1217-1227(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RAC1, TISSUE SPECIFICITY.
[10]	"Evolutionarily conserved human targets of adenosine to inosine RNA editing." Levanon E.Y., Hallegger M., Kinar Y., Shemesh R., Djinovic-Carugo K., Rechavi G., Jantsch M.F., Eisenberg E. Nucleic Acids Res. 33:1162-1168(2005) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING OF POSITION 320.
[11]	"CYFIP2, a direct p53 target, is leptomycin-B sensitive." Jackson R.S. II, Cho Y.-J., Stein S., Liang P. Cell Cycle 6:95-103(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[12]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-1062, MASS SPECTROMETRY.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF160973 mRNA. Translation: AAD45723.1. AB032994 mRNA. Translation: BAA86482.2. Different initiation. AL136549 mRNA. Translation: CAB66484.1. AK223604 mRNA. Translation: BAD97324.1. Different initiation. AC008676 Genomic DNA. No translation available. AC009185 Genomic DNA. No translation available. AC016571 Genomic DNA. No translation available. CH471062 Genomic DNA. Translation: EAW61604.1. CH471062 Genomic DNA. Translation: EAW61605.1. CH471062 Genomic DNA. Translation: EAW61606.1. BC011762 mRNA. Translation: AAH11762.1.
IPI	IPI00719600. IPI00789699.
PIR	T46248.
RefSeq	NP_001032409.2. NM_001037332.2. NP_001032410.1. NM_001037333.1. NP_055191.2. NM_014376.2.
UniGene	Hs.519702.
3D structure databases
ProteinModelPortal	Q96F07.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96F07. 11 interactions.
MINT	MINT-203551.
STRING	9606.ENSP00000390948.
PTM databases
PhosphoSite	Q96F07.
Polymorphism databases
DMDM	134034199.
Proteomic databases
PaxDb	Q96F07.
PRIDE	Q96F07.
Protocols and materials databases
DNASU	26999.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000442283; ENSP00000390948; ENSG00000055163.
GeneID	26999.
KEGG	hsa:26999.
UCSC	uc003lwt.3. human. uc021ygm.1. human.
Organism-specific databases
CTD	26999.
GeneCards	GC05P156674.
HGNC	HGNC:13760. CYFIP2.
HPA	CAB055519.
MIM	606323. gene.
neXtProt	NX_Q96F07.
PharmGKB	PA38368.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG306641.
HOVERGEN	HBG053209.
InParanoid	Q96F07.
KO	K05749.
OrthoDB	EOG4R7V8X.
Gene expression databases
ArrayExpress	Q96F07.
Bgee	Q96F07.
CleanEx	HS_CYFIP2.
Genevestigator	Q96F07.
Family and domain databases
InterPro	IPR008081. Cytoplasmic_FMR1-int. IPR016536. Cytoplasmic_FMR1-int_sub. [Graphical view]
PANTHER	PTHR12195. PTHR12195. 1 hit.
Pfam	PF05994. FragX_IP. 1 hit. [Graphical view]
PIRSF	PIRSF008153. FMR1_interacting. 1 hit.
PRINTS	PR01698. CYTOFMRPINTP.
ProtoNet	Search...
Other
ChiTaRS	CYFIP2. human.
GenomeRNAi	26999.
NextBio	49478.
SOURCE	Search...

Entry information

Entry name

CYFP2_HUMAN

Accession

Primary (citable) accession number: Q96F07
Secondary accession number(s): A6NLT2 , D3DQJ3, Q53EN5, Q9NTK4, Q9ULQ2, Q9UN29

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.