Q96EY9 (ADAT3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-specific adenosine deaminase-like protein 3

Alternative name(s):
tRNA-specific adenosine-34 deaminase subunit ADAT3

Gene names

Name:

ADAT3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in deamination of adenosine-34 to inosine in many tRNAs as a regulatory subunit Potential.
Cofactor	Zinc By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.2
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family. ADAT3 subfamily.
Caution	Val-225 is present instead of the conserved Glu which is an active site in the cytidine and deoxycytidylate deaminase family of enzymes. It is suggested that this protein may act as a regulatory subunit.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Polymorphism
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydrolase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	tRNA-specific adenosine deaminase-like protein 3		PRO_0000287658
Sites
	Metal binding	223	1	Zinc By similarity
	Metal binding	291	1	Zinc By similarity
	Metal binding	294	1	Zinc By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.3
	Modified residue	167	1	Phosphothreonine Ref.2
Natural variations
	Natural variant	332	1	R → C in a breast cancer sample; somatic mutation. Ref.5		VAR_035804

Sequences

	Sequence		Length	Mass (Da)
	Q96EY9 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 6CE25F534BBE92F9	FASTA	351	38,071
	10 20 30 40 50 60 MEPAPGLVEQ PKCLEAGSPE PEPAPWQALP VLSEKQSGDV ELVLAYAAPV LDKRQTSRLL 70 80 90 100 110 120 KEVSALHPLP AQPHLKRVRP SRDAGSPHAL EMLLCLAGPA SGPRSLAELL PRPAVDPRGL 130 140 150 160 170 180 GQPFLVPVPA RPPLTRGQFE EARAHWPTSF HEDKQVTSAL AGRLFSTQER AAMQSHMERA 190 200 210 220 230 240 VWAARRAAAR GLRAVGAVVV DPASDRVLAT GHDCSCADNP LLHAVMVCVD LVARGQGRGT 250 260 270 280 290 300 YDFRPFPACS FAPAAAPQAV RAGAVRKLDA DEDGLPYLCT GYDLYVTREP CAMCAMALVH 310 320 330 340 350 ARILRVFYGA PSPDGALGTR FRIHARPDLN HRFQVFRGVL EEQCRWLDPD T

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[2]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-167, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[3]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[4]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-332.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC011824 mRNA. Translation: AAH11824.1.
IPI	IPI00061802.
RefSeq	NP_612431.1. NM_138422.1.
UniGene	Hs.144980.
3D structure databases
HSSP	HSSP built from PDB template 2A8N based on UniProtKB Q8UHJ4.
ProteinModelPortal	Q96EY9.
SMR	Q96EY9. Positions 173-350.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96EY9. 1 interaction.
STRING	Q96EY9.
PTM databases
PhosphoSite	Q96EY9.
Polymorphism databases
DMDM	74731634.
Proteomic databases
PRIDE	Q96EY9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000329478; ENSP00000332448; ENSG00000213638.
GeneID	113179.
KEGG	hsa:113179.
NMPDR	fig|9606.3.peg.15306.
UCSC	uc002luh.1. human.
Organism-specific databases
CTD	113179.
GeneCards	GC19P001906.
HGNC	HGNC:25151. ADAT3.
neXtProt	NX_Q96EY9.
PharmGKB	PA162375609.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000010706.
HOGENOM	HBG275080.
HOVERGEN	HBG098143.
InParanoid	Q96EY9.
OMA	MVCIDLV.
OrthoDB	EOG4B2SXK.
PhylomeDB	Q96EY9.
Gene expression databases
ArrayExpress	Q96EY9.
Bgee	Q96EY9.
Genevestigator	Q96EY9.
Family and domain databases
InterPro	IPR002125. CMP_dCMP_Zn-bd. IPR016193. Cytidine_deaminase-like. [Graphical view]
KO	K15442.
Pfam	PF00383. dCMP_cyt_deam_1. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit.
PROSITE	PS00903. CYT_DCMP_DEAMINASES. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	78777.

Entry information

Entry name

ADAT3_HUMAN

Accession

Primary (citable) accession number: Q96EY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.