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Protein

Arylsulfatase G

Gene

ARSG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Displays arylsulfatase activity at acidic pH with pseudosubstrates, such as p-nitrocatechol sulfate and also, but with lower activity, p-nitrophenyl sulfate and 4-methylumbelliferyl sulfate.1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Enzyme regulationi

Inhibited by phosphate. The phosphate forms a covalent bond with the active site 3-oxoalanine.

Kineticsi

  1. KM=4.2 mM for p-nitrocatechol sulfate1 Publication
  1. Vmax=63.5 µmol/min/mg enzyme toward p-nitrocatechol sulfate1 Publication

pH dependencei

Optimum pH is 5.4.1 Publication

Temperature dependencei

Most efficient at 45-50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44CalciumBy similarity1
Metal bindingi45CalciumBy similarity1
Metal bindingi84Calcium; via 3-oxoalanineBy similarity1
Binding sitei137SubstrateBy similarity1
Active sitei139By similarity1
Binding sitei162SubstrateBy similarity1
Binding sitei251SubstrateBy similarity1
Metal bindingi302CalciumBy similarity1
Metal bindingi303CalciumBy similarity1

GO - Molecular functioni

  • arylsulfatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:HS06814-MONOMER.
ReactomeiR-HSA-1660662. Glycosphingolipid metabolism.
R-HSA-1663150. The activation of arylsulfatases.
SABIO-RKQ96EG1.

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase G (EC:3.1.6.-)
Short name:
ASG
Gene namesi
Name:ARSG
Synonyms:KIAA1001
ORF Names:UNQ839/PRO1777
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:24102. ARSG.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: HGNC
  • endoplasmic reticulum lumen Source: Reactome
  • extracellular space Source: UniProtKB
  • lysosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi84C → A: No sulfatase activity. 1 Publication1
Mutagenesisi501A → P: Decrease of sulfatase activity. 1 Publication1

Organism-specific databases

DisGeNETi22901.
OpenTargetsiENSG00000141337.
PharmGKBiPA143485307.

Chemistry databases

ChEMBLiCHEMBL2189124.

Polymorphism and mutation databases

BioMutaiARSG.
DMDMi74731559.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000004221517 – 525Arylsulfatase GAdd BLAST509

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei843-oxoalanine (Cys)1 Publication1
Glycosylationi117N-linked (GlcNAc...)Sequence analysis1
Glycosylationi215N-linked (GlcNAc...)Sequence analysis1
Glycosylationi356N-linked (GlcNAc...)Sequence analysis1
Glycosylationi497N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.
The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity
Glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ96EG1.
PaxDbiQ96EG1.
PeptideAtlasiQ96EG1.
PRIDEiQ96EG1.

PTM databases

iPTMnetiQ96EG1.
PhosphoSitePlusiQ96EG1.

Expressioni

Tissue specificityi

Widely expressed, with very low expression in brain, lung, heart and skeletal muscle.2 Publications

Gene expression databases

BgeeiENSG00000141337.
CleanExiHS_ARSG.
ExpressionAtlasiQ96EG1. baseline and differential.
GenevisibleiQ96EG1. HS.

Organism-specific databases

HPAiHPA023245.
HPA023285.

Interactioni

Protein-protein interaction databases

BioGridi116565. 21 interactors.
STRINGi9606.ENSP00000407193.

Structurei

3D structure databases

ProteinModelPortaliQ96EG1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3867. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00760000119062.
HOGENOMiHOG000135352.
HOVERGENiHBG004283.
InParanoidiQ96EG1.
KOiK12381.
OMAiCCNPYQI.
OrthoDBiEOG091G041Y.
PhylomeDBiQ96EG1.
TreeFamiTF314186.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96EG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGWLFLKVLL AGVSFSGFLY PLVDFCISGK TRGQKPNFVI ILADDMGWGD
60 70 80 90 100
LGANWAETKD TANLDKMASE GMRFVDFHAA ASTCSPSRAS LLTGRLGLRN
110 120 130 140 150
GVTRNFAVTS VGGLPLNETT LAEVLQQAGY VTGIIGKWHL GHHGSYHPNF
160 170 180 190 200
RGFDYYFGIP YSHDMGCTDT PGYNHPPCPA CPQGDGPSRN LQRDCYTDVA
210 220 230 240 250
LPLYENLNIV EQPVNLSSLA QKYAEKATQF IQRASTSGRP FLLYVALAHM
260 270 280 290 300
HVPLPVTQLP AAPRGRSLYG AGLWEMDSLV GQIKDKVDHT VKENTFLWFT
310 320 330 340 350
GDNGPWAQKC ELAGSVGPFT GFWQTRQGGS PAKQTTWEGG HRVPALAYWP
360 370 380 390 400
GRVPVNVTST ALLSVLDIFP TVVALAQASL PQGRRFDGVD VSEVLFGRSQ
410 420 430 440 450
PGHRVLFHPN SGAAGEFGAL QTVRLERYKA FYITGGARAC DGSTGPELQH
460 470 480 490 500
KFPLIFNLED DTAEAVPLER GGAEYQAVLP EVRKVLADVL QDIANDNISS
510 520
ADYTQDPSVT PCCNPYQIAC RCQAA
Length:525
Mass (Da):57,061
Last modified:December 1, 2001 - v1
Checksum:iADAB673A02B25754
GO

Sequence cautioni

The sequence BAA76845 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti444 – 445TG → MV in AAQ88746 (PubMed:12975309).Curated2
Sequence conflicti501A → P in BAA76845 (PubMed:10231032).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05251111A → V.Corresponds to variant rs8074806dbSNPEnsembl.1
Natural variantiVAR_052512236T → S.1 PublicationCorresponds to variant rs1558876dbSNPEnsembl.1
Natural variantiVAR_052513274W → R.1 PublicationCorresponds to variant rs1558878dbSNPEnsembl.1
Natural variantiVAR_074038326R → G.1 PublicationCorresponds to variant rs144503106dbSNPEnsembl.1
Natural variantiVAR_052514385R → H.1 PublicationCorresponds to variant rs9972951dbSNPEnsembl.1
Natural variantiVAR_074039398R → W.1 PublicationCorresponds to variant rs11657051dbSNPEnsembl.1
Natural variantiVAR_074040444T → M.1 PublicationCorresponds to variant rs62000424dbSNPEnsembl.1
Natural variantiVAR_074041481E → K.1 PublicationCorresponds to variant rs370852507dbSNPEnsembl.1
Natural variantiVAR_074042493I → T.1 PublicationCorresponds to variant rs61999318dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023218 mRNA. Translation: BAA76845.2. Different initiation.
AY358380 mRNA. Translation: AAQ88746.1.
BC012375 mRNA. Translation: AAH12375.1.
CCDSiCCDS11676.1.
RefSeqiNP_001254656.1. NM_001267727.1.
NP_055775.2. NM_014960.4.
XP_005257227.1. XM_005257170.3.
XP_016879850.1. XM_017024361.1.
XP_016879851.1. XM_017024362.1.
XP_016879852.1. XM_017024363.1.
XP_016879853.1. XM_017024364.1.
XP_016879854.1. XM_017024365.1.
UniGeneiHs.437249.

Genome annotation databases

EnsembliENST00000448504; ENSP00000407193; ENSG00000141337.
ENST00000621439; ENSP00000480910; ENSG00000141337.
GeneIDi22901.
KEGGihsa:22901.
UCSCiuc002jhc.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023218 mRNA. Translation: BAA76845.2. Different initiation.
AY358380 mRNA. Translation: AAQ88746.1.
BC012375 mRNA. Translation: AAH12375.1.
CCDSiCCDS11676.1.
RefSeqiNP_001254656.1. NM_001267727.1.
NP_055775.2. NM_014960.4.
XP_005257227.1. XM_005257170.3.
XP_016879850.1. XM_017024361.1.
XP_016879851.1. XM_017024362.1.
XP_016879852.1. XM_017024363.1.
XP_016879853.1. XM_017024364.1.
XP_016879854.1. XM_017024365.1.
UniGeneiHs.437249.

3D structure databases

ProteinModelPortaliQ96EG1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116565. 21 interactors.
STRINGi9606.ENSP00000407193.

Chemistry databases

ChEMBLiCHEMBL2189124.

PTM databases

iPTMnetiQ96EG1.
PhosphoSitePlusiQ96EG1.

Polymorphism and mutation databases

BioMutaiARSG.
DMDMi74731559.

Proteomic databases

EPDiQ96EG1.
PaxDbiQ96EG1.
PeptideAtlasiQ96EG1.
PRIDEiQ96EG1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000448504; ENSP00000407193; ENSG00000141337.
ENST00000621439; ENSP00000480910; ENSG00000141337.
GeneIDi22901.
KEGGihsa:22901.
UCSCiuc002jhc.3. human.

Organism-specific databases

CTDi22901.
DisGeNETi22901.
GeneCardsiARSG.
HGNCiHGNC:24102. ARSG.
HPAiHPA023245.
HPA023285.
MIMi610008. gene.
neXtProtiNX_Q96EG1.
OpenTargetsiENSG00000141337.
PharmGKBiPA143485307.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3867. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00760000119062.
HOGENOMiHOG000135352.
HOVERGENiHBG004283.
InParanoidiQ96EG1.
KOiK12381.
OMAiCCNPYQI.
OrthoDBiEOG091G041Y.
PhylomeDBiQ96EG1.
TreeFamiTF314186.

Enzyme and pathway databases

BioCyciZFISH:HS06814-MONOMER.
ReactomeiR-HSA-1660662. Glycosphingolipid metabolism.
R-HSA-1663150. The activation of arylsulfatases.
SABIO-RKQ96EG1.

Miscellaneous databases

ChiTaRSiARSG. human.
GenomeRNAii22901.
PROiQ96EG1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141337.
CleanExiHS_ARSG.
ExpressionAtlasiQ96EG1. baseline and differential.
GenevisibleiQ96EG1. HS.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARSG_HUMAN
AccessioniPrimary (citable) accession number: Q96EG1
Secondary accession number(s): Q6UXF2, Q9Y2K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.