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Protein

Arylsulfatase G

Gene

ARSG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Displays arylsulfatase activity at acidic pH with pseudosubstrates, such as p-nitrocatechol sulfate and also, but with lower activity, p-nitrophenyl sulfate and 4-methylumbelliferyl sulfate.1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Enzyme regulationi

Inhibited by phosphate. The phosphate forms a covalent bond with the active site 3-oxoalanine.

Kineticsi

  1. KM=4.2 mM for p-nitrocatechol sulfate1 Publication
  1. Vmax=63.5 µmol/min/mg enzyme toward p-nitrocatechol sulfate1 Publication

pH dependencei

Optimum pH is 5.4.1 Publication

Temperature dependencei

Most efficient at 45-50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44CalciumBy similarity1
Metal bindingi45CalciumBy similarity1
Active sitei84Nucleophile1 Publication1
Metal bindingi84Calcium; via 3-oxoalanineBy similarity1
Binding sitei137SubstrateBy similarity1
Active sitei139By similarity1
Binding sitei162SubstrateBy similarity1
Binding sitei251SubstrateBy similarity1
Metal bindingi302CalciumBy similarity1
Metal bindingi303CalciumBy similarity1

GO - Molecular functioni

  • arylsulfatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1660662 Glycosphingolipid metabolism
R-HSA-1663150 The activation of arylsulfatases
SABIO-RKiQ96EG1

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase G (EC:3.1.6.-)
Short name:
ASG
Gene namesi
Name:ARSG
Synonyms:KIAA1001
ORF Names:UNQ839/PRO1777
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000141337.12
HGNCiHGNC:24102 ARSG
MIMi610008 gene
neXtProtiNX_Q96EG1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi84C → A: No sulfatase activity. 1 Publication1
Mutagenesisi501A → P: Decrease of sulfatase activity. 1 Publication1

Organism-specific databases

DisGeNETi22901
OpenTargetsiENSG00000141337
PharmGKBiPA143485307

Chemistry databases

ChEMBLiCHEMBL2189124

Polymorphism and mutation databases

BioMutaiARSG
DMDMi74731559

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000004221517 – 525Arylsulfatase GAdd BLAST509

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei843-oxoalanine (Cys)1 Publication1
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi215N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi356N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi497N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.
The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity
Glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ96EG1
PeptideAtlasiQ96EG1
PRIDEiQ96EG1

PTM databases

iPTMnetiQ96EG1
PhosphoSitePlusiQ96EG1

Expressioni

Tissue specificityi

Widely expressed, with very low expression in brain, lung, heart and skeletal muscle.2 Publications

Gene expression databases

BgeeiENSG00000141337
CleanExiHS_ARSG
ExpressionAtlasiQ96EG1 baseline and differential
GenevisibleiQ96EG1 HS

Organism-specific databases

HPAiHPA023245
HPA023285

Interactioni

Protein-protein interaction databases

BioGridi116565, 25 interactors
STRINGi9606.ENSP00000407193

Structurei

3D structure databases

ProteinModelPortaliQ96EG1
SMRiQ96EG1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3867 Eukaryota
COG3119 LUCA
GeneTreeiENSGT00760000119062
HOGENOMiHOG000135352
HOVERGENiHBG004283
InParanoidiQ96EG1
KOiK12381
OMAiKAFYITG
OrthoDBiEOG091G041Y
PhylomeDBiQ96EG1
TreeFamiTF314186

Family and domain databases

Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR024607 Sulfatase_CS
IPR000917 Sulfatase_N
PfamiView protein in Pfam
PF00884 Sulfatase, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00523 SULFATASE_1, 1 hit
PS00149 SULFATASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96EG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGWLFLKVLL AGVSFSGFLY PLVDFCISGK TRGQKPNFVI ILADDMGWGD
60 70 80 90 100
LGANWAETKD TANLDKMASE GMRFVDFHAA ASTCSPSRAS LLTGRLGLRN
110 120 130 140 150
GVTRNFAVTS VGGLPLNETT LAEVLQQAGY VTGIIGKWHL GHHGSYHPNF
160 170 180 190 200
RGFDYYFGIP YSHDMGCTDT PGYNHPPCPA CPQGDGPSRN LQRDCYTDVA
210 220 230 240 250
LPLYENLNIV EQPVNLSSLA QKYAEKATQF IQRASTSGRP FLLYVALAHM
260 270 280 290 300
HVPLPVTQLP AAPRGRSLYG AGLWEMDSLV GQIKDKVDHT VKENTFLWFT
310 320 330 340 350
GDNGPWAQKC ELAGSVGPFT GFWQTRQGGS PAKQTTWEGG HRVPALAYWP
360 370 380 390 400
GRVPVNVTST ALLSVLDIFP TVVALAQASL PQGRRFDGVD VSEVLFGRSQ
410 420 430 440 450
PGHRVLFHPN SGAAGEFGAL QTVRLERYKA FYITGGARAC DGSTGPELQH
460 470 480 490 500
KFPLIFNLED DTAEAVPLER GGAEYQAVLP EVRKVLADVL QDIANDNISS
510 520
ADYTQDPSVT PCCNPYQIAC RCQAA
Length:525
Mass (Da):57,061
Last modified:December 1, 2001 - v1
Checksum:iADAB673A02B25754
GO

Sequence cautioni

The sequence BAA76845 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti444 – 445TG → MV in AAQ88746 (PubMed:12975309).Curated2
Sequence conflicti501A → P in BAA76845 (PubMed:10231032).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05251111A → V. Corresponds to variant dbSNP:rs8074806Ensembl.1
Natural variantiVAR_052512236T → S1 PublicationCorresponds to variant dbSNP:rs1558876Ensembl.1
Natural variantiVAR_052513274W → R1 PublicationCorresponds to variant dbSNP:rs1558878Ensembl.1
Natural variantiVAR_074038326R → G1 PublicationCorresponds to variant dbSNP:rs144503106Ensembl.1
Natural variantiVAR_052514385R → H1 PublicationCorresponds to variant dbSNP:rs9972951Ensembl.1
Natural variantiVAR_074039398R → W1 PublicationCorresponds to variant dbSNP:rs11657051Ensembl.1
Natural variantiVAR_074040444T → M1 PublicationCorresponds to variant dbSNP:rs62000424Ensembl.1
Natural variantiVAR_074041481E → K1 PublicationCorresponds to variant dbSNP:rs370852507Ensembl.1
Natural variantiVAR_074042493I → T1 PublicationCorresponds to variant dbSNP:rs61999318Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023218 mRNA Translation: BAA76845.2 Different initiation.
AY358380 mRNA Translation: AAQ88746.1
BC012375 mRNA Translation: AAH12375.1
CCDSiCCDS11676.1
RefSeqiNP_001254656.1, NM_001267727.1
NP_055775.2, NM_014960.4
XP_005257227.1, XM_005257170.3
XP_016879850.1, XM_017024361.1
XP_016879851.1, XM_017024362.1
XP_016879852.1, XM_017024363.1
XP_016879853.1, XM_017024364.1
XP_016879854.1, XM_017024365.1
UniGeneiHs.437249
Hs.657130
Hs.668801

Genome annotation databases

EnsembliENST00000448504; ENSP00000407193; ENSG00000141337
ENST00000621439; ENSP00000480910; ENSG00000141337
GeneIDi22901
KEGGihsa:22901
UCSCiuc002jhc.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiARSG_HUMAN
AccessioniPrimary (citable) accession number: Q96EG1
Secondary accession number(s): Q6UXF2, Q9Y2K4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: December 1, 2001
Last modified: February 28, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health