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Protein

Arylsulfatase G

Gene

ARSG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Displays arylsulfatase activity at acidic pH with pseudosubstrates, such as p-nitrocatechol sulfate and also, but with lower activity, p-nitrophenyl sulfate and 4-methylumbelliferyl sulfate.1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Enzyme regulationi

Inhibited by phosphate. The phosphate forms a covalent bond with the active site 3-oxoalanine.

Kineticsi

  1. KM=4.2 mM for p-nitrocatechol sulfate1 Publication
  1. Vmax=63.5 µmol/min/mg enzyme toward p-nitrocatechol sulfate1 Publication

pH dependencei

Optimum pH is 5.4.1 Publication

Temperature dependencei

Most efficient at 45-50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441CalciumBy similarity
Metal bindingi45 – 451CalciumBy similarity
Metal bindingi84 – 841Calcium; via 3-oxoalanineBy similarity
Binding sitei137 – 1371SubstrateBy similarity
Active sitei139 – 1391By similarity
Binding sitei162 – 1621SubstrateBy similarity
Binding sitei251 – 2511SubstrateBy similarity
Metal bindingi302 – 3021CalciumBy similarity
Metal bindingi303 – 3031CalciumBy similarity

GO - Molecular functioni

  • arylsulfatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1660662. Glycosphingolipid metabolism.
R-HSA-1663150. The activation of arylsulfatases.
SABIO-RKQ96EG1.

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase G (EC:3.1.6.-)
Short name:
ASG
Gene namesi
Name:ARSG
Synonyms:KIAA1001
ORF Names:UNQ839/PRO1777
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:24102. ARSG.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: HGNC
  • endoplasmic reticulum lumen Source: Reactome
  • extracellular space Source: UniProtKB
  • lysosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi84 – 841C → A: No sulfatase activity. 1 Publication
Mutagenesisi501 – 5011A → P: Decrease of sulfatase activity. 1 Publication

Organism-specific databases

PharmGKBiPA143485307.

Chemistry

ChEMBLiCHEMBL2189124.

Polymorphism and mutation databases

BioMutaiARSG.
DMDMi74731559.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence analysisAdd
BLAST
Chaini17 – 525509Arylsulfatase GPRO_0000042215Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei84 – 8413-oxoalanine (Cys)1 Publication
Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence analysis
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence analysis
Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence analysis
Glycosylationi497 – 4971N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.
The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity
Glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ96EG1.
PaxDbiQ96EG1.
PeptideAtlasiQ96EG1.
PRIDEiQ96EG1.

PTM databases

iPTMnetiQ96EG1.
PhosphoSiteiQ96EG1.

Expressioni

Tissue specificityi

Widely expressed, with very low expression in brain, lung, heart and skeletal muscle.2 Publications

Gene expression databases

BgeeiENSG00000141337.
CleanExiHS_ARSG.
ExpressionAtlasiQ96EG1. baseline and differential.
GenevisibleiQ96EG1. HS.

Organism-specific databases

HPAiHPA023245.
HPA023285.

Interactioni

Protein-protein interaction databases

BioGridi116565. 21 interactions.
STRINGi9606.ENSP00000407193.

Structurei

3D structure databases

ProteinModelPortaliQ96EG1.
SMRiQ96EG1. Positions 33-515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3867. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00760000119062.
HOGENOMiHOG000135352.
HOVERGENiHBG004283.
InParanoidiQ96EG1.
KOiK12381.
OMAiCCNPYQI.
OrthoDBiEOG091G041Y.
PhylomeDBiQ96EG1.
TreeFamiTF314186.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96EG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGWLFLKVLL AGVSFSGFLY PLVDFCISGK TRGQKPNFVI ILADDMGWGD
60 70 80 90 100
LGANWAETKD TANLDKMASE GMRFVDFHAA ASTCSPSRAS LLTGRLGLRN
110 120 130 140 150
GVTRNFAVTS VGGLPLNETT LAEVLQQAGY VTGIIGKWHL GHHGSYHPNF
160 170 180 190 200
RGFDYYFGIP YSHDMGCTDT PGYNHPPCPA CPQGDGPSRN LQRDCYTDVA
210 220 230 240 250
LPLYENLNIV EQPVNLSSLA QKYAEKATQF IQRASTSGRP FLLYVALAHM
260 270 280 290 300
HVPLPVTQLP AAPRGRSLYG AGLWEMDSLV GQIKDKVDHT VKENTFLWFT
310 320 330 340 350
GDNGPWAQKC ELAGSVGPFT GFWQTRQGGS PAKQTTWEGG HRVPALAYWP
360 370 380 390 400
GRVPVNVTST ALLSVLDIFP TVVALAQASL PQGRRFDGVD VSEVLFGRSQ
410 420 430 440 450
PGHRVLFHPN SGAAGEFGAL QTVRLERYKA FYITGGARAC DGSTGPELQH
460 470 480 490 500
KFPLIFNLED DTAEAVPLER GGAEYQAVLP EVRKVLADVL QDIANDNISS
510 520
ADYTQDPSVT PCCNPYQIAC RCQAA
Length:525
Mass (Da):57,061
Last modified:December 1, 2001 - v1
Checksum:iADAB673A02B25754
GO

Sequence cautioni

The sequence BAA76845 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti444 – 4452TG → MV in AAQ88746 (PubMed:12975309).Curated
Sequence conflicti501 – 5011A → P in BAA76845 (PubMed:10231032).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti11 – 111A → V.
Corresponds to variant rs8074806 [ dbSNP | Ensembl ].
VAR_052511
Natural varianti236 – 2361T → S.1 Publication
Corresponds to variant rs1558876 [ dbSNP | Ensembl ].
VAR_052512
Natural varianti274 – 2741W → R.1 Publication
Corresponds to variant rs1558878 [ dbSNP | Ensembl ].
VAR_052513
Natural varianti326 – 3261R → G.1 Publication
Corresponds to variant rs144503106 [ dbSNP | Ensembl ].
VAR_074038
Natural varianti385 – 3851R → H.1 Publication
Corresponds to variant rs9972951 [ dbSNP | Ensembl ].
VAR_052514
Natural varianti398 – 3981R → W.1 Publication
Corresponds to variant rs11657051 [ dbSNP | Ensembl ].
VAR_074039
Natural varianti444 – 4441T → M.1 Publication
Corresponds to variant rs62000424 [ dbSNP | Ensembl ].
VAR_074040
Natural varianti481 – 4811E → K.1 Publication
Corresponds to variant rs370852507 [ dbSNP | Ensembl ].
VAR_074041
Natural varianti493 – 4931I → T.1 Publication
Corresponds to variant rs61999318 [ dbSNP | Ensembl ].
VAR_074042

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023218 mRNA. Translation: BAA76845.2. Different initiation.
AY358380 mRNA. Translation: AAQ88746.1.
BC012375 mRNA. Translation: AAH12375.1.
CCDSiCCDS11676.1.
RefSeqiNP_001254656.1. NM_001267727.1.
NP_055775.2. NM_014960.4.
XP_005257227.1. XM_005257170.3.
UniGeneiHs.437249.

Genome annotation databases

EnsembliENST00000448504; ENSP00000407193; ENSG00000141337.
ENST00000621439; ENSP00000480910; ENSG00000141337.
GeneIDi22901.
KEGGihsa:22901.
UCSCiuc002jhc.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023218 mRNA. Translation: BAA76845.2. Different initiation.
AY358380 mRNA. Translation: AAQ88746.1.
BC012375 mRNA. Translation: AAH12375.1.
CCDSiCCDS11676.1.
RefSeqiNP_001254656.1. NM_001267727.1.
NP_055775.2. NM_014960.4.
XP_005257227.1. XM_005257170.3.
UniGeneiHs.437249.

3D structure databases

ProteinModelPortaliQ96EG1.
SMRiQ96EG1. Positions 33-515.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116565. 21 interactions.
STRINGi9606.ENSP00000407193.

Chemistry

ChEMBLiCHEMBL2189124.

PTM databases

iPTMnetiQ96EG1.
PhosphoSiteiQ96EG1.

Polymorphism and mutation databases

BioMutaiARSG.
DMDMi74731559.

Proteomic databases

EPDiQ96EG1.
PaxDbiQ96EG1.
PeptideAtlasiQ96EG1.
PRIDEiQ96EG1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000448504; ENSP00000407193; ENSG00000141337.
ENST00000621439; ENSP00000480910; ENSG00000141337.
GeneIDi22901.
KEGGihsa:22901.
UCSCiuc002jhc.3. human.

Organism-specific databases

CTDi22901.
GeneCardsiARSG.
HGNCiHGNC:24102. ARSG.
HPAiHPA023245.
HPA023285.
MIMi610008. gene.
neXtProtiNX_Q96EG1.
PharmGKBiPA143485307.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3867. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00760000119062.
HOGENOMiHOG000135352.
HOVERGENiHBG004283.
InParanoidiQ96EG1.
KOiK12381.
OMAiCCNPYQI.
OrthoDBiEOG091G041Y.
PhylomeDBiQ96EG1.
TreeFamiTF314186.

Enzyme and pathway databases

ReactomeiR-HSA-1660662. Glycosphingolipid metabolism.
R-HSA-1663150. The activation of arylsulfatases.
SABIO-RKQ96EG1.

Miscellaneous databases

ChiTaRSiARSG. human.
GenomeRNAii22901.
PROiQ96EG1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141337.
CleanExiHS_ARSG.
ExpressionAtlasiQ96EG1. baseline and differential.
GenevisibleiQ96EG1. HS.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARSG_HUMAN
AccessioniPrimary (citable) accession number: Q96EG1
Secondary accession number(s): Q6UXF2, Q9Y2K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.