Q96ED9 (HOOK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Hook homolog 2
Short name=h-hook2
Short name=hHK2

Gene names

Name:

HOOK2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones. Ref.5 Ref.6 Ref.7
Subunit structure	Self-associates. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with AKTIP/FTS, HOOK1 and HOOK3. Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect. Interacts with CEP110. Interacts with microtubules. Interacts with ZC3H14. Ref.4 Ref.6 Ref.7 Ref.10
Subcellular location	Cytoplasm › cytoskeleton › centrosome. Cytoplasm. Cytoplasm › cytoskeleton. Note: Colocalizes with aggresomes, which are aggregates of misfolded proteins, at the centrosome. Also localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules. Ref.4 Ref.5 Ref.6
Sequence similarities	Belongs to the hook family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	early endosome to late endosome transport Inferred from mutant phenotype Ref.7. Source: UniProtKB endocytosis Traceable author statement Ref.1. Source: ProtInc endosome organization Inferred from mutant phenotype Ref.7. Source: UniProtKB endosome to lysosome transport Inferred from mutant phenotype Ref.7. Source: UniProtKB lysosome organization Inferred from mutant phenotype Ref.7. Source: UniProtKB microtubule cytoskeleton organization Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	FHF complex Inferred from direct assay Ref.7. Source: UniProtKB centrosome Inferred from direct assay Ref.6. Source: UniProtKB microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CCDC33	Q8N5R6	2	EBI-743290,EBI-740841

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96ED9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96ED9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     534-535: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	Protein Hook homolog 2		PRO_0000219194
Regions
	Region	1 – 548	548	Sufficient for interaction with microtubules
	Region	1 – 161	161	Required for localization to the centrosome and induction of aggresome formation
	Region	533 – 719	187	Required for localization to the centrosome and induction of aggresome formation
	Region	584 – 719	136	Sufficient for interaction with CEP110
	Coiled coil	180 – 427	248	Potential
	Coiled coil	455 – 607	153	Potential
Amino acid modifications
	Modified residue	163	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	534 – 535	2	Missing in isoform 2.		VSP_009342
	Natural variant	10	1	G → R. Corresponds to variant rs2305376 [ dbSNP | Ensembl ].		VAR_017575
	Natural variant	488	1	H → Q. Ref.3 Corresponds to variant rs897804 [ dbSNP | Ensembl ].		VAR_017576
Experimental info
	Sequence conflict	376	1	R → L in AAC09299. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: FDF5F8A53DEE9DB6	FASTA	719	83,207
	10 20 30 40 50 60 MSVDKAELCG SLLTWLQTFH VPSPCASPQD LSSGLAVAYV LNQIDPSWFN EAWLQGISED 70 80 90 100 110 120 PGPNWKLKVS NLKMVLRSLV EYSQDVLAHP VSEEHLPDVS LIGEFSDPAE LGKLLQLVLG 130 140 150 160 170 180 CAISCEKKQD HIQRIMTLEE SVQHVVMEAI QELMTKDTPD SLSPETYGNF DSQSRRYYFL 190 200 210 220 230 240 SEEAEEGDEL QQRCLDLERQ LMLLSEEKQS LAQENAGLRE RMGRPEGEGT PGLTAKKLLL 250 260 270 280 290 300 LQSQLEQLQE ENFRLESGRE DERLRCAELE REVAELQHRN QALTSLAQEA QALKDEMDEL 310 320 330 340 350 360 RQSSERAGQL EATLTSCRRR LGELRELRRQ VRQLEERNAG HAERTRQLED ELRRAGSLRA 370 380 390 400 410 420 QLEAQRRQVQ ELQGQRQEEA MKAEKWLFEC RNLEEKYESV TKEKERLLAE RDSLREANEE 430 440 450 460 470 480 LRCAQLQPRG LTQADPSLDP TSTPVDNLAA EILPAELRET LLRLQLENKR LCRQEAADRE 490 500 510 520 530 540 RQEELQRHLE DANRARHGLE TQHRLNQQQL SELRAQVEDL QKALQEQGGK TEDAISILLK 550 560 570 580 590 600 RKLEEHLQKL HEADLELQRK REYIEELEPP TDSSTARRIE ELQHNLQKKD ADLRAMEERY 610 620 630 640 650 660 RRYVDKARMV MQTMEPKQRP AAGAPPELHS LRTQLRERDV RIRHLEMDFE KSRSQREQEE 670 680 690 700 710 KLLISAWYNM GMALQQRAGE ERAPAHAQSF LAQQRLATNS RRGPLGRLAS LNLRPTDKH
	Isoform 2 [UniParc]. Checksum: 980FCF8BA96E6E70	FASTA	717	83,022
	10 20 30 40 50 60 MSVDKAELCG SLLTWLQTFH VPSPCASPQD LSSGLAVAYV LNQIDPSWFN EAWLQGISED 70 80 90 100 110 120 PGPNWKLKVS NLKMVLRSLV EYSQDVLAHP VSEEHLPDVS LIGEFSDPAE LGKLLQLVLG 130 140 150 160 170 180 CAISCEKKQD HIQRIMTLEE SVQHVVMEAI QELMTKDTPD SLSPETYGNF DSQSRRYYFL 190 200 210 220 230 240 SEEAEEGDEL QQRCLDLERQ LMLLSEEKQS LAQENAGLRE RMGRPEGEGT PGLTAKKLLL 250 260 270 280 290 300 LQSQLEQLQE ENFRLESGRE DERLRCAELE REVAELQHRN QALTSLAQEA QALKDEMDEL 310 320 330 340 350 360 RQSSERAGQL EATLTSCRRR LGELRELRRQ VRQLEERNAG HAERTRQLED ELRRAGSLRA 370 380 390 400 410 420 QLEAQRRQVQ ELQGQRQEEA MKAEKWLFEC RNLEEKYESV TKEKERLLAE RDSLREANEE 430 440 450 460 470 480 LRCAQLQPRG LTQADPSLDP TSTPVDNLAA EILPAELRET LLRLQLENKR LCRQEAADRE 490 500 510 520 530 540 RQEELQRHLE DANRARHGLE TQHRLNQQQL SELRAQVEDL QKALQEQGGK TEDSILLKRK 550 560 570 580 590 600 LEEHLQKLHE ADLELQRKRE YIEELEPPTD SSTARRIEEL QHNLQKKDAD LRAMEERYRR 610 620 630 640 650 660 YVDKARMVMQ TMEPKQRPAA GAPPELHSLR TQLRERDVRI RHLEMDFEKS RSQREQEEKL 670 680 690 700 710 LISAWYNMGM ALQQRAGEER APAHAQSFLA QQRLATNSRR GPLGRLASLN LRPTDKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic analysis of hook, a gene required for endocytic trafficking in Drosophila." Kraemer H., Phistry M. Genetics 151:675-684(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLN-488. Tissue: Ovary.
[4]	"The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins." Walenta J.H., Didier A.J., Liu X., Kraemer H. J. Cell Biol. 152:923-934(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MICROTUBULES, SUBCELLULAR LOCATION.
[5]	"Hook2 contributes to aggresome formation." Szebenyi G., Wigley W.C., Hall B., Didier A.J., Yu M., Thomas P., Kraemer H. BMC Cell Biol. 8:19-19(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function." Szebenyi G., Hall B., Yu R., Hashim A.I., Kraemer H. Traffic 8:32-46(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CEP110, SUBCELLULAR LOCATION.
[7]	"An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex." Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W. Mol. Biol. Cell 19:5059-5071(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FHF COMPLEX, FUNCTION, SELF-ASSOCIATION, INTERACTION WITH AKTIP; HOOK1; HOOK3; VPS16 AND VPS41.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"SUT-2 potentiates tau-induced neurotoxicity in Caenorhabditis elegans." Guthrie C.R., Schellenberg G.D., Kraemer B.C. Hum. Mol. Genet. 18:1825-1838(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ZC3H14.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF044924 mRNA. Translation: AAC09299.1. AC018761 Genomic DNA. No translation available. BC012443 mRNA. Translation: AAH12443.1.
IPI	IPI00002432. IPI00398585.
RefSeq	NP_001093646.1. NM_001100176.1. NP_037444.2. NM_013312.2.
UniGene	Hs.30792.
3D structure databases
ProteinModelPortal	Q96ED9.
SMR	Q96ED9. Positions 8-161.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96ED9. 18 interactions.
MINT	MINT-1433957.
STRING	9606.ENSP00000380785.
PTM databases
PhosphoSite	Q96ED9.
Polymorphism databases
DMDM	296439323.
Proteomic databases
PaxDb	Q96ED9.
PRIDE	Q96ED9.
Protocols and materials databases
DNASU	29911.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264827; ENSP00000264827; ENSG00000095066. ENST00000397668; ENSP00000380785; ENSG00000095066.
GeneID	29911.
KEGG	hsa:29911.
UCSC	uc002muy.2. human. uc002muz.2. human.
Organism-specific databases
CTD	29911.
GeneCards	GC19M012873.
H-InvDB	HIX0014803.
HGNC	HGNC:19885. HOOK2.
HPA	HPA043519. HPA050351.
MIM	607824. gene.
neXtProt	NX_Q96ED9.
PharmGKB	PA134986864.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG302169.
HOGENOM	HOG000294112.
HOVERGEN	HBG051920.
InParanoid	Q96ED9.
KO	K16611.
OMA	LEPKQRP.
Gene expression databases
Bgee	Q96ED9.
CleanEx	HS_HOOK2.
Genevestigator	Q96ED9.
GermOnline	ENSG00000095066. Homo sapiens.
Family and domain databases
InterPro	IPR008636. HOOK/CCDC88. [Graphical view]
Pfam	PF05622. HOOK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HOOK2. human.
GenomeRNAi	29911.
NextBio	52496.
SOURCE	Search...

Entry information

Entry name

HOOK2_HUMAN

Accession

Primary (citable) accession number: Q96ED9
Secondary accession number(s): O60562

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.