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Q96ED9

- HOOK2_HUMAN

UniProt

Q96ED9 - HOOK2_HUMAN

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Protein

Protein Hook homolog 2

Gene

HOOK2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones.3 Publications

GO - Molecular functioni

  1. identical protein binding Source: UniProtKB

GO - Biological processi

  1. early endosome to late endosome transport Source: UniProtKB
  2. endocytosis Source: ProtInc
  3. endosome organization Source: UniProtKB
  4. endosome to lysosome transport Source: UniProtKB
  5. lysosome organization Source: UniProtKB
  6. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Hook homolog 2
Short name:
h-hook2
Short name:
hHK2
Gene namesi
Name:HOOK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:19885. HOOK2.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Cytoplasm. Cytoplasmcytoskeleton
Note: Colocalizes with aggresomes, which are aggregates of misfolded proteins, at the centrosome. Also localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. FHF complex Source: UniProtKB
  3. microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134986864.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719Protein Hook homolog 2PRO_0000219194Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei163 – 1631Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96ED9.
PaxDbiQ96ED9.
PRIDEiQ96ED9.

PTM databases

PhosphoSiteiQ96ED9.

Expressioni

Gene expression databases

BgeeiQ96ED9.
CleanExiHS_HOOK2.
ExpressionAtlasiQ96ED9. baseline and differential.
GenevestigatoriQ96ED9.

Organism-specific databases

HPAiHPA043519.
HPA050351.

Interactioni

Subunit structurei

Self-associates. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with AKTIP/FTS, HOOK1 and HOOK3. Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect. Interacts with CNTRL. Interacts with microtubules. Interacts with ZC3H14.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC33Q8N5R62EBI-743290,EBI-740841

Protein-protein interaction databases

BioGridi118957. 33 interactions.
IntActiQ96ED9. 23 interactions.
MINTiMINT-1433957.
STRINGi9606.ENSP00000380785.

Structurei

3D structure databases

ProteinModelPortaliQ96ED9.
SMRiQ96ED9. Positions 8-161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 548548Sufficient for interaction with microtubulesAdd
BLAST
Regioni1 – 161161Required for localization to the centrosome and induction of aggresome formationAdd
BLAST
Regioni533 – 719187Required for localization to the centrosome and induction of aggresome formationAdd
BLAST
Regioni584 – 719136Sufficient for interaction with CNTRLAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili180 – 427248Sequence AnalysisAdd
BLAST
Coiled coili455 – 607153Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the hook family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG302169.
GeneTreeiENSGT00690000101702.
HOGENOMiHOG000294112.
HOVERGENiHBG051920.
InParanoidiQ96ED9.
KOiK16611.
OMAiTNTRRGP.
OrthoDBiEOG7ZD1V5.
PhylomeDBiQ96ED9.
TreeFamiTF320231.

Family and domain databases

InterProiIPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 1 hit.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96ED9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVDKAELCG SLLTWLQTFH VPSPCASPQD LSSGLAVAYV LNQIDPSWFN
60 70 80 90 100
EAWLQGISED PGPNWKLKVS NLKMVLRSLV EYSQDVLAHP VSEEHLPDVS
110 120 130 140 150
LIGEFSDPAE LGKLLQLVLG CAISCEKKQD HIQRIMTLEE SVQHVVMEAI
160 170 180 190 200
QELMTKDTPD SLSPETYGNF DSQSRRYYFL SEEAEEGDEL QQRCLDLERQ
210 220 230 240 250
LMLLSEEKQS LAQENAGLRE RMGRPEGEGT PGLTAKKLLL LQSQLEQLQE
260 270 280 290 300
ENFRLESGRE DERLRCAELE REVAELQHRN QALTSLAQEA QALKDEMDEL
310 320 330 340 350
RQSSERAGQL EATLTSCRRR LGELRELRRQ VRQLEERNAG HAERTRQLED
360 370 380 390 400
ELRRAGSLRA QLEAQRRQVQ ELQGQRQEEA MKAEKWLFEC RNLEEKYESV
410 420 430 440 450
TKEKERLLAE RDSLREANEE LRCAQLQPRG LTQADPSLDP TSTPVDNLAA
460 470 480 490 500
EILPAELRET LLRLQLENKR LCRQEAADRE RQEELQRHLE DANRARHGLE
510 520 530 540 550
TQHRLNQQQL SELRAQVEDL QKALQEQGGK TEDAISILLK RKLEEHLQKL
560 570 580 590 600
HEADLELQRK REYIEELEPP TDSSTARRIE ELQHNLQKKD ADLRAMEERY
610 620 630 640 650
RRYVDKARMV MQTMEPKQRP AAGAPPELHS LRTQLRERDV RIRHLEMDFE
660 670 680 690 700
KSRSQREQEE KLLISAWYNM GMALQQRAGE ERAPAHAQSF LAQQRLATNS
710
RRGPLGRLAS LNLRPTDKH
Length:719
Mass (Da):83,207
Last modified:May 18, 2010 - v3
Checksum:iFDF5F8A53DEE9DB6
GO
Isoform 2 (identifier: Q96ED9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     534-535: Missing.

Show »
Length:717
Mass (Da):83,022
Checksum:i980FCF8BA96E6E70
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti376 – 3761R → L in AAC09299. (PubMed:9927460)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti10 – 101G → R.
Corresponds to variant rs2305376 [ dbSNP | Ensembl ].
VAR_017575
Natural varianti488 – 4881H → Q.1 Publication
Corresponds to variant rs897804 [ dbSNP | Ensembl ].
VAR_017576

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei534 – 5352Missing in isoform 2. 1 PublicationVSP_009342

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044924 mRNA. Translation: AAC09299.1.
AC018761 Genomic DNA. No translation available.
BC012443 mRNA. Translation: AAH12443.1.
CCDSiCCDS42507.1. [Q96ED9-2]
CCDS42508.1. [Q96ED9-1]
RefSeqiNP_001093646.1. NM_001100176.1. [Q96ED9-2]
NP_037444.2. NM_013312.2. [Q96ED9-1]
UniGeneiHs.30792.

Genome annotation databases

EnsembliENST00000264827; ENSP00000264827; ENSG00000095066. [Q96ED9-2]
ENST00000397668; ENSP00000380785; ENSG00000095066. [Q96ED9-1]
GeneIDi29911.
KEGGihsa:29911.
UCSCiuc002muy.2. human. [Q96ED9-1]
uc002muz.2. human. [Q96ED9-2]

Polymorphism databases

DMDMi296439323.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044924 mRNA. Translation: AAC09299.1 .
AC018761 Genomic DNA. No translation available.
BC012443 mRNA. Translation: AAH12443.1 .
CCDSi CCDS42507.1. [Q96ED9-2 ]
CCDS42508.1. [Q96ED9-1 ]
RefSeqi NP_001093646.1. NM_001100176.1. [Q96ED9-2 ]
NP_037444.2. NM_013312.2. [Q96ED9-1 ]
UniGenei Hs.30792.

3D structure databases

ProteinModelPortali Q96ED9.
SMRi Q96ED9. Positions 8-161.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 118957. 33 interactions.
IntActi Q96ED9. 23 interactions.
MINTi MINT-1433957.
STRINGi 9606.ENSP00000380785.

PTM databases

PhosphoSitei Q96ED9.

Polymorphism databases

DMDMi 296439323.

Proteomic databases

MaxQBi Q96ED9.
PaxDbi Q96ED9.
PRIDEi Q96ED9.

Protocols and materials databases

DNASUi 29911.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000264827 ; ENSP00000264827 ; ENSG00000095066 . [Q96ED9-2 ]
ENST00000397668 ; ENSP00000380785 ; ENSG00000095066 . [Q96ED9-1 ]
GeneIDi 29911.
KEGGi hsa:29911.
UCSCi uc002muy.2. human. [Q96ED9-1 ]
uc002muz.2. human. [Q96ED9-2 ]

Organism-specific databases

CTDi 29911.
GeneCardsi GC19M012873.
H-InvDB HIX0014803.
HGNCi HGNC:19885. HOOK2.
HPAi HPA043519.
HPA050351.
MIMi 607824. gene.
neXtProti NX_Q96ED9.
PharmGKBi PA134986864.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG302169.
GeneTreei ENSGT00690000101702.
HOGENOMi HOG000294112.
HOVERGENi HBG051920.
InParanoidi Q96ED9.
KOi K16611.
OMAi TNTRRGP.
OrthoDBi EOG7ZD1V5.
PhylomeDBi Q96ED9.
TreeFami TF320231.

Miscellaneous databases

ChiTaRSi HOOK2. human.
GeneWikii HOOK2.
GenomeRNAii 29911.
NextBioi 52496.
PROi Q96ED9.
SOURCEi Search...

Gene expression databases

Bgeei Q96ED9.
CleanExi HS_HOOK2.
ExpressionAtlasi Q96ED9. baseline and differential.
Genevestigatori Q96ED9.

Family and domain databases

InterProi IPR008636. Hook-related_fam.
[Graphical view ]
PANTHERi PTHR18947. PTHR18947. 1 hit.
Pfami PF05622. HOOK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic analysis of hook, a gene required for endocytic trafficking in Drosophila."
    Kraemer H., Phistry M.
    Genetics 151:675-684(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLN-488.
    Tissue: Ovary.
  4. "The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins."
    Walenta J.H., Didier A.J., Liu X., Kraemer H.
    J. Cell Biol. 152:923-934(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MICROTUBULES, SUBCELLULAR LOCATION.
  5. Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function."
    Szebenyi G., Hall B., Yu R., Hashim A.I., Kraemer H.
    Traffic 8:32-46(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CNTRL, SUBCELLULAR LOCATION.
  7. "An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex."
    Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W.
    Mol. Biol. Cell 19:5059-5071(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FHF COMPLEX, FUNCTION, SELF-ASSOCIATION, INTERACTION WITH AKTIP; HOOK1; HOOK3; VPS16 AND VPS41.
  8. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "SUT-2 potentiates tau-induced neurotoxicity in Caenorhabditis elegans."
    Guthrie C.R., Schellenberg G.D., Kraemer B.C.
    Hum. Mol. Genet. 18:1825-1838(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZC3H14.

Entry informationi

Entry nameiHOOK2_HUMAN
AccessioniPrimary (citable) accession number: Q96ED9
Secondary accession number(s): O60562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: May 18, 2010
Last modified: October 29, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3