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Q96ED9

- HOOK2_HUMAN

UniProt

Q96ED9 - HOOK2_HUMAN

Protein

Protein Hook homolog 2

Gene

HOOK2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 3 (18 May 2010)
      Previous versions | rss
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    Functioni

    Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones.3 Publications

    GO - Molecular functioni

    1. identical protein binding Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. early endosome to late endosome transport Source: UniProtKB
    2. endocytosis Source: ProtInc
    3. endosome organization Source: UniProtKB
    4. endosome to lysosome transport Source: UniProtKB
    5. lysosome organization Source: UniProtKB
    6. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein Hook homolog 2
    Short name:
    h-hook2
    Short name:
    hHK2
    Gene namesi
    Name:HOOK2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:19885. HOOK2.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Cytoplasm. Cytoplasmcytoskeleton
    Note: Colocalizes with aggresomes, which are aggregates of misfolded proteins, at the centrosome. Also localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. FHF complex Source: UniProtKB
    3. microtubule Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134986864.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 719719Protein Hook homolog 2PRO_0000219194Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei163 – 1631Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ96ED9.
    PaxDbiQ96ED9.
    PRIDEiQ96ED9.

    PTM databases

    PhosphoSiteiQ96ED9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ96ED9.
    BgeeiQ96ED9.
    CleanExiHS_HOOK2.
    GenevestigatoriQ96ED9.

    Organism-specific databases

    HPAiHPA043519.
    HPA050351.

    Interactioni

    Subunit structurei

    Self-associates. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with AKTIP/FTS, HOOK1 and HOOK3. Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect. Interacts with CNTRL. Interacts with microtubules. Interacts with ZC3H14.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CCDC33Q8N5R62EBI-743290,EBI-740841

    Protein-protein interaction databases

    BioGridi118957. 24 interactions.
    IntActiQ96ED9. 23 interactions.
    MINTiMINT-1433957.
    STRINGi9606.ENSP00000380785.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96ED9.
    SMRiQ96ED9. Positions 8-161.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 548548Sufficient for interaction with microtubulesAdd
    BLAST
    Regioni1 – 161161Required for localization to the centrosome and induction of aggresome formationAdd
    BLAST
    Regioni533 – 719187Required for localization to the centrosome and induction of aggresome formationAdd
    BLAST
    Regioni584 – 719136Sufficient for interaction with CNTRLAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili180 – 427248Sequence AnalysisAdd
    BLAST
    Coiled coili455 – 607153Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the hook family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG302169.
    HOGENOMiHOG000294112.
    HOVERGENiHBG051920.
    InParanoidiQ96ED9.
    KOiK16611.
    OMAiTNTRRGP.
    OrthoDBiEOG7ZD1V5.
    PhylomeDBiQ96ED9.
    TreeFamiTF320231.

    Family and domain databases

    InterProiIPR008636. Hook-related_fam.
    [Graphical view]
    PANTHERiPTHR18947. PTHR18947. 1 hit.
    PfamiPF05622. HOOK. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96ED9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSVDKAELCG SLLTWLQTFH VPSPCASPQD LSSGLAVAYV LNQIDPSWFN    50
    EAWLQGISED PGPNWKLKVS NLKMVLRSLV EYSQDVLAHP VSEEHLPDVS 100
    LIGEFSDPAE LGKLLQLVLG CAISCEKKQD HIQRIMTLEE SVQHVVMEAI 150
    QELMTKDTPD SLSPETYGNF DSQSRRYYFL SEEAEEGDEL QQRCLDLERQ 200
    LMLLSEEKQS LAQENAGLRE RMGRPEGEGT PGLTAKKLLL LQSQLEQLQE 250
    ENFRLESGRE DERLRCAELE REVAELQHRN QALTSLAQEA QALKDEMDEL 300
    RQSSERAGQL EATLTSCRRR LGELRELRRQ VRQLEERNAG HAERTRQLED 350
    ELRRAGSLRA QLEAQRRQVQ ELQGQRQEEA MKAEKWLFEC RNLEEKYESV 400
    TKEKERLLAE RDSLREANEE LRCAQLQPRG LTQADPSLDP TSTPVDNLAA 450
    EILPAELRET LLRLQLENKR LCRQEAADRE RQEELQRHLE DANRARHGLE 500
    TQHRLNQQQL SELRAQVEDL QKALQEQGGK TEDAISILLK RKLEEHLQKL 550
    HEADLELQRK REYIEELEPP TDSSTARRIE ELQHNLQKKD ADLRAMEERY 600
    RRYVDKARMV MQTMEPKQRP AAGAPPELHS LRTQLRERDV RIRHLEMDFE 650
    KSRSQREQEE KLLISAWYNM GMALQQRAGE ERAPAHAQSF LAQQRLATNS 700
    RRGPLGRLAS LNLRPTDKH 719
    Length:719
    Mass (Da):83,207
    Last modified:May 18, 2010 - v3
    Checksum:iFDF5F8A53DEE9DB6
    GO
    Isoform 2 (identifier: Q96ED9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         534-535: Missing.

    Show »
    Length:717
    Mass (Da):83,022
    Checksum:i980FCF8BA96E6E70
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti376 – 3761R → L in AAC09299. (PubMed:9927460)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti10 – 101G → R.
    Corresponds to variant rs2305376 [ dbSNP | Ensembl ].
    VAR_017575
    Natural varianti488 – 4881H → Q.1 Publication
    Corresponds to variant rs897804 [ dbSNP | Ensembl ].
    VAR_017576

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei534 – 5352Missing in isoform 2. 1 PublicationVSP_009342

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF044924 mRNA. Translation: AAC09299.1.
    AC018761 Genomic DNA. No translation available.
    BC012443 mRNA. Translation: AAH12443.1.
    CCDSiCCDS42507.1. [Q96ED9-2]
    CCDS42508.1. [Q96ED9-1]
    RefSeqiNP_001093646.1. NM_001100176.1. [Q96ED9-2]
    NP_037444.2. NM_013312.2. [Q96ED9-1]
    UniGeneiHs.30792.

    Genome annotation databases

    EnsembliENST00000264827; ENSP00000264827; ENSG00000095066. [Q96ED9-2]
    ENST00000397668; ENSP00000380785; ENSG00000095066. [Q96ED9-1]
    GeneIDi29911.
    KEGGihsa:29911.
    UCSCiuc002muy.2. human. [Q96ED9-1]
    uc002muz.2. human. [Q96ED9-2]

    Polymorphism databases

    DMDMi296439323.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF044924 mRNA. Translation: AAC09299.1 .
    AC018761 Genomic DNA. No translation available.
    BC012443 mRNA. Translation: AAH12443.1 .
    CCDSi CCDS42507.1. [Q96ED9-2 ]
    CCDS42508.1. [Q96ED9-1 ]
    RefSeqi NP_001093646.1. NM_001100176.1. [Q96ED9-2 ]
    NP_037444.2. NM_013312.2. [Q96ED9-1 ]
    UniGenei Hs.30792.

    3D structure databases

    ProteinModelPortali Q96ED9.
    SMRi Q96ED9. Positions 8-161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 118957. 24 interactions.
    IntActi Q96ED9. 23 interactions.
    MINTi MINT-1433957.
    STRINGi 9606.ENSP00000380785.

    PTM databases

    PhosphoSitei Q96ED9.

    Polymorphism databases

    DMDMi 296439323.

    Proteomic databases

    MaxQBi Q96ED9.
    PaxDbi Q96ED9.
    PRIDEi Q96ED9.

    Protocols and materials databases

    DNASUi 29911.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000264827 ; ENSP00000264827 ; ENSG00000095066 . [Q96ED9-2 ]
    ENST00000397668 ; ENSP00000380785 ; ENSG00000095066 . [Q96ED9-1 ]
    GeneIDi 29911.
    KEGGi hsa:29911.
    UCSCi uc002muy.2. human. [Q96ED9-1 ]
    uc002muz.2. human. [Q96ED9-2 ]

    Organism-specific databases

    CTDi 29911.
    GeneCardsi GC19M012873.
    H-InvDB HIX0014803.
    HGNCi HGNC:19885. HOOK2.
    HPAi HPA043519.
    HPA050351.
    MIMi 607824. gene.
    neXtProti NX_Q96ED9.
    PharmGKBi PA134986864.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG302169.
    HOGENOMi HOG000294112.
    HOVERGENi HBG051920.
    InParanoidi Q96ED9.
    KOi K16611.
    OMAi TNTRRGP.
    OrthoDBi EOG7ZD1V5.
    PhylomeDBi Q96ED9.
    TreeFami TF320231.

    Miscellaneous databases

    ChiTaRSi HOOK2. human.
    GeneWikii HOOK2.
    GenomeRNAii 29911.
    NextBioi 52496.
    PROi Q96ED9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96ED9.
    Bgeei Q96ED9.
    CleanExi HS_HOOK2.
    Genevestigatori Q96ED9.

    Family and domain databases

    InterProi IPR008636. Hook-related_fam.
    [Graphical view ]
    PANTHERi PTHR18947. PTHR18947. 1 hit.
    Pfami PF05622. HOOK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic analysis of hook, a gene required for endocytic trafficking in Drosophila."
      Kraemer H., Phistry M.
      Genetics 151:675-684(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLN-488.
      Tissue: Ovary.
    4. "The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins."
      Walenta J.H., Didier A.J., Liu X., Kraemer H.
      J. Cell Biol. 152:923-934(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MICROTUBULES, SUBCELLULAR LOCATION.
    5. Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function."
      Szebenyi G., Hall B., Yu R., Hashim A.I., Kraemer H.
      Traffic 8:32-46(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CNTRL, SUBCELLULAR LOCATION.
    7. "An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex."
      Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W.
      Mol. Biol. Cell 19:5059-5071(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FHF COMPLEX, FUNCTION, SELF-ASSOCIATION, INTERACTION WITH AKTIP; HOOK1; HOOK3; VPS16 AND VPS41.
    8. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "SUT-2 potentiates tau-induced neurotoxicity in Caenorhabditis elegans."
      Guthrie C.R., Schellenberg G.D., Kraemer B.C.
      Hum. Mol. Genet. 18:1825-1838(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZC3H14.

    Entry informationi

    Entry nameiHOOK2_HUMAN
    AccessioniPrimary (citable) accession number: Q96ED9
    Secondary accession number(s): O60562
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 2, 2004
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 103 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3