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Q96ED9 (HOOK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein Hook homolog 2

Short name=h-hook2
Short name=hHK2
Gene names
Name:HOOK2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length719 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones. Ref.5 Ref.6 Ref.7

Subunit structure

Self-associates. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with AKTIP/FTS, HOOK1 and HOOK3. Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect. Interacts with CNTRL. Interacts with microtubules. Interacts with ZC3H14. Ref.4 Ref.6 Ref.7 Ref.10

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Cytoplasm. Cytoplasmcytoskeleton. Note: Colocalizes with aggresomes, which are aggregates of misfolded proteins, at the centrosome. Also localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules. Ref.4 Ref.5 Ref.6

Sequence similarities

Belongs to the hook family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CCDC33Q8N5R62EBI-743290,EBI-740841

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96ED9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96ED9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     534-535: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 719719Protein Hook homolog 2
PRO_0000219194

Regions

Region1 – 548548Sufficient for interaction with microtubules
Region1 – 161161Required for localization to the centrosome and induction of aggresome formation
Region533 – 719187Required for localization to the centrosome and induction of aggresome formation
Region584 – 719136Sufficient for interaction with CNTRL
Coiled coil180 – 427248 Potential
Coiled coil455 – 607153 Potential

Amino acid modifications

Modified residue1631Phosphoserine Ref.9

Natural variations

Alternative sequence534 – 5352Missing in isoform 2.
VSP_009342
Natural variant101G → R.
Corresponds to variant rs2305376 [ dbSNP | Ensembl ].
VAR_017575
Natural variant4881H → Q. Ref.3
Corresponds to variant rs897804 [ dbSNP | Ensembl ].
VAR_017576

Experimental info

Sequence conflict3761R → L in AAC09299. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: FDF5F8A53DEE9DB6

FASTA71983,207
        10         20         30         40         50         60 
MSVDKAELCG SLLTWLQTFH VPSPCASPQD LSSGLAVAYV LNQIDPSWFN EAWLQGISED 

        70         80         90        100        110        120 
PGPNWKLKVS NLKMVLRSLV EYSQDVLAHP VSEEHLPDVS LIGEFSDPAE LGKLLQLVLG 

       130        140        150        160        170        180 
CAISCEKKQD HIQRIMTLEE SVQHVVMEAI QELMTKDTPD SLSPETYGNF DSQSRRYYFL 

       190        200        210        220        230        240 
SEEAEEGDEL QQRCLDLERQ LMLLSEEKQS LAQENAGLRE RMGRPEGEGT PGLTAKKLLL 

       250        260        270        280        290        300 
LQSQLEQLQE ENFRLESGRE DERLRCAELE REVAELQHRN QALTSLAQEA QALKDEMDEL 

       310        320        330        340        350        360 
RQSSERAGQL EATLTSCRRR LGELRELRRQ VRQLEERNAG HAERTRQLED ELRRAGSLRA 

       370        380        390        400        410        420 
QLEAQRRQVQ ELQGQRQEEA MKAEKWLFEC RNLEEKYESV TKEKERLLAE RDSLREANEE 

       430        440        450        460        470        480 
LRCAQLQPRG LTQADPSLDP TSTPVDNLAA EILPAELRET LLRLQLENKR LCRQEAADRE 

       490        500        510        520        530        540 
RQEELQRHLE DANRARHGLE TQHRLNQQQL SELRAQVEDL QKALQEQGGK TEDAISILLK 

       550        560        570        580        590        600 
RKLEEHLQKL HEADLELQRK REYIEELEPP TDSSTARRIE ELQHNLQKKD ADLRAMEERY 

       610        620        630        640        650        660 
RRYVDKARMV MQTMEPKQRP AAGAPPELHS LRTQLRERDV RIRHLEMDFE KSRSQREQEE 

       670        680        690        700        710 
KLLISAWYNM GMALQQRAGE ERAPAHAQSF LAQQRLATNS RRGPLGRLAS LNLRPTDKH 

« Hide

Isoform 2 [UniParc].

Checksum: 980FCF8BA96E6E70
Show »

FASTA71783,022

References

« Hide 'large scale' references
[1]"Genetic analysis of hook, a gene required for endocytic trafficking in Drosophila."
Kraemer H., Phistry M.
Genetics 151:675-684(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLN-488.
Tissue: Ovary.
[4]"The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins."
Walenta J.H., Didier A.J., Liu X., Kraemer H.
J. Cell Biol. 152:923-934(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MICROTUBULES, SUBCELLULAR LOCATION.
[5]"Hook2 contributes to aggresome formation."
Szebenyi G., Wigley W.C., Hall B., Didier A.J., Yu M., Thomas P., Kraemer H.
BMC Cell Biol. 8:19-19(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function."
Szebenyi G., Hall B., Yu R., Hashim A.I., Kraemer H.
Traffic 8:32-46(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CNTRL, SUBCELLULAR LOCATION.
[7]"An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex."
Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W.
Mol. Biol. Cell 19:5059-5071(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FHF COMPLEX, FUNCTION, SELF-ASSOCIATION, INTERACTION WITH AKTIP; HOOK1; HOOK3; VPS16 AND VPS41.
[8]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"SUT-2 potentiates tau-induced neurotoxicity in Caenorhabditis elegans."
Guthrie C.R., Schellenberg G.D., Kraemer B.C.
Hum. Mol. Genet. 18:1825-1838(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZC3H14.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF044924 mRNA. Translation: AAC09299.1.
AC018761 Genomic DNA. No translation available.
BC012443 mRNA. Translation: AAH12443.1.
RefSeqNP_001093646.1. NM_001100176.1.
NP_037444.2. NM_013312.2.
UniGeneHs.30792.

3D structure databases

ProteinModelPortalQ96ED9.
SMRQ96ED9. Positions 8-161.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118957. 24 interactions.
IntActQ96ED9. 23 interactions.
MINTMINT-1433957.
STRING9606.ENSP00000380785.

PTM databases

PhosphoSiteQ96ED9.

Polymorphism databases

DMDM296439323.

Proteomic databases

PaxDbQ96ED9.
PRIDEQ96ED9.

Protocols and materials databases

DNASU29911.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264827; ENSP00000264827; ENSG00000095066. [Q96ED9-2]
ENST00000397668; ENSP00000380785; ENSG00000095066. [Q96ED9-1]
GeneID29911.
KEGGhsa:29911.
UCSCuc002muy.2. human. [Q96ED9-1]
uc002muz.2. human. [Q96ED9-2]

Organism-specific databases

CTD29911.
GeneCardsGC19M012873.
H-InvDBHIX0014803.
HGNCHGNC:19885. HOOK2.
HPAHPA043519.
HPA050351.
MIM607824. gene.
neXtProtNX_Q96ED9.
PharmGKBPA134986864.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG302169.
HOGENOMHOG000294112.
HOVERGENHBG051920.
InParanoidQ96ED9.
KOK16611.
OMATNTRRGP.
OrthoDBEOG7ZD1V5.
TreeFamTF320231.

Gene expression databases

ArrayExpressQ96ED9.
BgeeQ96ED9.
CleanExHS_HOOK2.
GenevestigatorQ96ED9.

Family and domain databases

InterProIPR008636. Hook-related_fam.
[Graphical view]
PANTHERPTHR18947. PTHR18947. 1 hit.
PfamPF05622. HOOK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHOOK2. human.
GeneWikiHOOK2.
GenomeRNAi29911.
NextBio52496.
PROQ96ED9.
SOURCESearch...

Entry information

Entry nameHOOK2_HUMAN
AccessionPrimary (citable) accession number: Q96ED9
Secondary accession number(s): O60562
Entry history
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: May 18, 2010
Last modified: March 19, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM