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Protein

Killer cell lectin-like receptor subfamily G member 1

Gene

KLRG1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells.1 Publication

GO - Molecular functioni

  • carbohydrate binding Source: ProtInc
  • receptor activity Source: ProtInc

GO - Biological processi

  • cell surface receptor signaling pathway Source: ProtInc
  • cellular defense response Source: ProtInc
  • inflammatory response Source: ProtInc
  • innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
Killer cell lectin-like receptor subfamily G member 1
Alternative name(s):
C-type lectin domain family 15 member A
ITIM-containing receptor MAFA-L
MAFA-like receptor
Mast cell function-associated antigen
Gene namesi
Name:KLRG1
Synonyms:CLEC15A, MAFA, MAFAL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:6380. KLRG1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3838CytoplasmicSequence analysisAdd
BLAST
Transmembranei39 – 5921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini60 – 195136ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: ProtInc
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30170.

Polymorphism and mutation databases

BioMutaiKLRG1.
DMDMi74731536.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Killer cell lectin-like receptor subfamily G member 1PRO_0000331256Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi65 – 651N-linked (GlcNAc...)Sequence analysis
Disulfide bondi75 ↔ 86PROSITE-ProRule annotation1 Publication
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence analysis
Disulfide bondi103 ↔ 184PROSITE-ProRule annotation1 Publication
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence analysis
Glycosylationi150 – 1501N-linked (GlcNAc...)Sequence analysis
Disulfide bondi163 ↔ 176PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ96E93.
PaxDbiQ96E93.
PeptideAtlasiQ96E93.
PRIDEiQ96E93.

PTM databases

iPTMnetiQ96E93.
PhosphoSiteiQ96E93.

Expressioni

Tissue specificityi

Expressed specifically on natural killer (NK) cells and T-cells, mainly CD8 T-cells.2 Publications

Inductioni

By pathogens and viruses infections.1 Publication

Gene expression databases

BgeeiQ96E93.
CleanExiHS_KLRG1.
HS_MAFA.
ExpressionAtlasiQ96E93. baseline and differential.
GenevisibleiQ96E93. HS.

Organism-specific databases

HPAiHPA051403.

Interactioni

Subunit structurei

Forms a monomer and homodimer; disulfide-linked. Interacts (via ITIM motif) with PTPN11 and INPP5D.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SYNE4Q8N2053EBI-750770,EBI-7131783

Protein-protein interaction databases

BioGridi115514. 2 interactions.
IntActiQ96E93. 2 interactions.
MINTiMINT-1465786.
STRINGi9606.ENSP00000349477.

Structurei

Secondary structure

1
195
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi80 – 823Combined sources
Beta strandi85 – 895Combined sources
Helixi96 – 1049Combined sources
Turni105 – 1073Combined sources
Helixi116 – 1238Combined sources
Beta strandi131 – 14313Combined sources
Beta strandi163 – 1675Combined sources
Beta strandi170 – 1745Combined sources
Beta strandi180 – 1856Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FF7X-ray1.80C/D75-186[»]
ProteinModelPortaliQ96E93.
SMRiQ96E93. Positions 75-186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96E93.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 185104C-type lectinPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi5 – 106ITIM motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 314Poly-Ser

Domaini

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00840000129990.
HOGENOMiHOG000082429.
HOVERGENiHBG103557.
InParanoidiQ96E93.
KOiK10076.
OMAiFCLARDS.
PhylomeDBiQ96E93.
TreeFamiTF336674.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96E93-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDSVIYSML ELPTATQAQN DYGPQQKSSS SRPSCSCLVA IALGLLTAVL
60 70 80 90 100
LSVLLYQWIL CQGSNYSTCA SCPSCPDRWM KYGNHCYYFS VEEKDWNSSL
110 120 130 140 150
EFCLARDSHL LVITDNQEMS LLQVFLSEAF CWIGLRNNSG WRWEDGSPLN
160 170 180 190
FSRISSNSFV QTCGAINKNG LQASSCEVPL HWVCKKCPFA DQALF
Length:195
Mass (Da):21,831
Last modified:December 1, 2001 - v1
Checksum:i178EE98E08EEC473
GO
Isoform 2 (identifier: Q96E93-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     187-195: CPFADQALF → VRL

Show »
Length:189
Mass (Da):21,206
Checksum:iFA9023F1523656A8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321R → K in AAC34731 (PubMed:9765598).Curated
Sequence conflicti42 – 421A → T in AAC34731 (PubMed:9765598).Curated
Sequence conflicti182 – 1821W → G in AAC34731 (PubMed:9765598).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti58 – 581W → R.
Corresponds to variant rs1805749 [ dbSNP | Ensembl ].
VAR_042750

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei187 – 1959CPFADQALF → VRL in isoform 2. 3 PublicationsVSP_033151

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034952 mRNA. Translation: AAC34731.1.
AF081675 mRNA. Translation: AAC32200.1.
AF097358 mRNA. Translation: AAD03719.1.
AK316337 mRNA. Translation: BAH14708.1.
CH471116 Genomic DNA. Translation: EAW88594.1.
CH471116 Genomic DNA. Translation: EAW88595.1.
BC012621 mRNA. Translation: AAH12621.1.
CCDSiCCDS8599.1. [Q96E93-2]
RefSeqiNP_005801.3. NM_005810.3. [Q96E93-2]
UniGeneiHs.558446.

Genome annotation databases

EnsembliENST00000266551; ENSP00000266551; ENSG00000139187. [Q96E93-1]
ENST00000356986; ENSP00000349477; ENSG00000139187. [Q96E93-2]
GeneIDi10219.
KEGGihsa:10219.
UCSCiuc001qvg.4. human. [Q96E93-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034952 mRNA. Translation: AAC34731.1.
AF081675 mRNA. Translation: AAC32200.1.
AF097358 mRNA. Translation: AAD03719.1.
AK316337 mRNA. Translation: BAH14708.1.
CH471116 Genomic DNA. Translation: EAW88594.1.
CH471116 Genomic DNA. Translation: EAW88595.1.
BC012621 mRNA. Translation: AAH12621.1.
CCDSiCCDS8599.1. [Q96E93-2]
RefSeqiNP_005801.3. NM_005810.3. [Q96E93-2]
UniGeneiHs.558446.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FF7X-ray1.80C/D75-186[»]
ProteinModelPortaliQ96E93.
SMRiQ96E93. Positions 75-186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115514. 2 interactions.
IntActiQ96E93. 2 interactions.
MINTiMINT-1465786.
STRINGi9606.ENSP00000349477.

PTM databases

iPTMnetiQ96E93.
PhosphoSiteiQ96E93.

Polymorphism and mutation databases

BioMutaiKLRG1.
DMDMi74731536.

Proteomic databases

MaxQBiQ96E93.
PaxDbiQ96E93.
PeptideAtlasiQ96E93.
PRIDEiQ96E93.

Protocols and materials databases

DNASUi10219.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000266551; ENSP00000266551; ENSG00000139187. [Q96E93-1]
ENST00000356986; ENSP00000349477; ENSG00000139187. [Q96E93-2]
GeneIDi10219.
KEGGihsa:10219.
UCSCiuc001qvg.4. human. [Q96E93-1]

Organism-specific databases

CTDi10219.
GeneCardsiKLRG1.
HGNCiHGNC:6380. KLRG1.
HPAiHPA051403.
MIMi604874. gene.
neXtProtiNX_Q96E93.
PharmGKBiPA30170.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00840000129990.
HOGENOMiHOG000082429.
HOVERGENiHBG103557.
InParanoidiQ96E93.
KOiK10076.
OMAiFCLARDS.
PhylomeDBiQ96E93.
TreeFamiTF336674.

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

EvolutionaryTraceiQ96E93.
GeneWikiiKLRG1.
GenomeRNAii10219.
PROiQ96E93.
SOURCEiSearch...

Gene expression databases

BgeeiQ96E93.
CleanExiHS_KLRG1.
HS_MAFA.
ExpressionAtlasiQ96E93. baseline and differential.
GenevisibleiQ96E93. HS.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "MAFA-L, an ITIM-containing receptor encoded by the human NK cell gene complex and expressed by basophils and NK cells."
    Butcher S., Arney K.L., Cook G.P.
    Eur. J. Immunol. 28:3755-3762(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Spleen.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Bone marrow.
  6. "Expression of killer cell lectin-like receptor G1 on antigen-specific human CD8+ T lymphocytes during active, latent, and resolved infection and its relation with CD57."
    Ibegbu C.C., Xu Y.X., Harris W., Maggio D., Miller J.D., Kourtis A.P.
    J. Immunol. 174:6088-6094(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION BY VIRUSES AND PATHOGENS INFECTIONS.
  7. "Tumor-associated E-cadherin mutations affect binding to the killer cell lectin-like receptor G1 in humans."
    Schwartzkopff S., Gruendemann C., Schweier O., Rosshart S., Karjalainen K.E., Becker K.-F., Pircher H.
    J. Immunol. 179:1022-1029(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: LIGAND-BINDING.
  8. "Structure of natural killer cell receptor KLRG1 bound to E-cadherin reveals basis for MHC-independent missing self recognition."
    Li Y., Hofmann M., Wang Q., Teng L., Chlewicki L.K., Pircher H., Mariuzza R.A.
    Immunity 31:35-46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 75-186 IN COMPLEX WITH CDH1, FUNCTION, SUBCELLULAR LOCATION, DISULFIDE BONDS.

Entry informationi

Entry nameiKLRG1_HUMAN
AccessioniPrimary (citable) accession number: Q96E93
Secondary accession number(s): B7ZAM2, O43198, O75613
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.