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Q96E93 (KLRG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Killer cell lectin-like receptor subfamily G member 1
Alternative name(s):
C-type lectin domain family 15 member A
ITIM-containing receptor MAFA-L
MAFA-like receptor
Mast cell function-associated antigen
Gene names
Name:KLRG1
Synonyms:CLEC15A, MAFA, MAFAL
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length195 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells. Ref.8

Subunit structure

Forms monomer and homodimer; disulfide-linked By similarity. Interacts (via ITIM motif) with PTPN11 and INPP5D By similarity. Ref.8

Subcellular location

Cell membrane; Single-pass type II membrane protein Ref.8.

Tissue specificity

Expressed specifically on natural killer (NK) cells and T-cells, mainly CD8 T-cells. Ref.2 Ref.6

Induction

By pathogens and viruses infections. Ref.6

Domain

Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. Upon phosphorylation of ITIM motif KLRG1 associates with the two phosphatases PTPN11 and INPP5D By similarity.

Sequence similarities

Contains 1 C-type lectin domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96E93-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96E93-2)

The sequence of this isoform differs from the canonical sequence as follows:
     187-195: CPFADQALF → VRL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 195195Killer cell lectin-like receptor subfamily G member 1
PRO_0000331256

Regions

Topological domain1 – 3838Cytoplasmic Potential
Transmembrane39 – 5921Helical; Signal-anchor for type II membrane protein; Potential
Topological domain60 – 195136Extracellular Potential
Domain82 – 185104C-type lectin
Motif5 – 106ITIM motif
Compositional bias28 – 314Poly-Ser

Amino acid modifications

Glycosylation651N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation1371N-linked (GlcNAc...) Potential
Glycosylation1501N-linked (GlcNAc...) Potential
Disulfide bond75 ↔ 86 Ref.8
Disulfide bond103 ↔ 184 Ref.8
Disulfide bond163 ↔ 176 Ref.8

Natural variations

Alternative sequence187 – 1959CPFADQALF → VRL in isoform 2.
VSP_033151
Natural variant581W → R.
Corresponds to variant rs1805749 [ dbSNP | Ensembl ].
VAR_042750

Experimental info

Sequence conflict321R → K in AAC34731. Ref.1
Sequence conflict421A → T in AAC34731. Ref.1
Sequence conflict1821W → G in AAC34731. Ref.1

Secondary structure

.......... 195
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 178EE98E08EEC473

FASTA19521,831
        10         20         30         40         50         60 
MTDSVIYSML ELPTATQAQN DYGPQQKSSS SRPSCSCLVA IALGLLTAVL LSVLLYQWIL 

        70         80         90        100        110        120 
CQGSNYSTCA SCPSCPDRWM KYGNHCYYFS VEEKDWNSSL EFCLARDSHL LVITDNQEMS 

       130        140        150        160        170        180 
LLQVFLSEAF CWIGLRNNSG WRWEDGSPLN FSRISSNSFV QTCGAINKNG LQASSCEVPL 

       190 
HWVCKKCPFA DQALF 

« Hide

Isoform 2 [UniParc].

Checksum: FA9023F1523656A8
Show »

FASTA18921,206

References

« Hide 'large scale' references
[1]"Human MAFA has alternatively spliced variants."
Lamers M.B.A.C., Lamont A.G., Williams D.H.
Biochim. Biophys. Acta 1399:209-212(1998) [PubMed: 9765598] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"MAFA-L, an ITIM-containing receptor encoded by the human NK cell gene complex and expressed by basophils and NK cells."
Butcher S., Arney K.L., Cook G.P.
Eur. J. Immunol. 28:3755-3762(1998) [PubMed: 9842918] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Spleen.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Bone marrow.
[6]"Expression of killer cell lectin-like receptor G1 on antigen-specific human CD8+ T lymphocytes during active, latent, and resolved infection and its relation with CD57."
Ibegbu C.C., Xu Y.X., Harris W., Maggio D., Miller J.D., Kourtis A.P.
J. Immunol. 174:6088-6094(2005) [PubMed: 15879103] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION BY VIRUSES AND PATHOGENS INFECTIONS.
[7]"Tumor-associated E-cadherin mutations affect binding to the killer cell lectin-like receptor G1 in humans."
Schwartzkopff S., Gruendemann C., Schweier O., Rosshart S., Karjalainen K.E., Becker K.-F., Pircher H.
J. Immunol. 179:1022-1029(2007) [PubMed: 17617594] [Abstract]
Cited for: LIGAND-BINDING.
[8]"Structure of natural killer cell receptor KLRG1 bound to E-cadherin reveals basis for MHC-independent missing self recognition."
Li Y., Hofmann M., Wang Q., Teng L., Chlewicki L.K., Pircher H., Mariuzza R.A.
Immunity 31:35-46(2009) [PubMed: 19604491] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 75-186 IN COMPLEX WITH CDH1, FUNCTION, SUBCELLULAR LOCATION, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF034952 mRNA. Translation: AAC34731.1.
AF081675 mRNA. Translation: AAC32200.1.
AF097358 mRNA. Translation: AAD03719.1.
AK316337 mRNA. Translation: BAH14708.1.
CH471116 Genomic DNA. Translation: EAW88594.1.
CH471116 Genomic DNA. Translation: EAW88595.1.
BC012621 mRNA. Translation: AAH12621.1.
IPIIPI00153018.
IPI00478141.
RefSeqNP_005801.3. NM_005810.3.
UniGeneHs.558446.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3FF7X-ray1.80C/D75-186[»]
ProteinModelPortalQ96E93.
SMRQ96E93. Positions 75-186.
ModBaseSearch...

Protein-protein interaction databases

IntActQ96E93. 1 interaction.
MINTMINT-1465786.
STRINGQ96E93.

Polymorphism databases

DMDM74731536.

Proteomic databases

PRIDEQ96E93.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000266551; ENSP00000266551; ENSG00000139187.
ENST00000356986; ENSP00000349477; ENSG00000139187.
GeneID10219.
KEGGhsa:10219.
UCSCuc001qvg.1. human.
uc001qvh.2. human.

Organism-specific databases

CTD10219.
GeneCardsGC12P009142.
HGNCHGNC:6380. KLRG1.
MIM604874. gene.
neXtProtNX_Q96E93.
PharmGKBPA30170.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG06786.
GeneTreeENSGT00550000074413.
HOGENOMHBG126616.
HOVERGENHBG103557.
InParanoidQ96E93.
OMAKDWNSSL.
OrthoDBEOG4ZS94B.
PhylomeDBQ96E93.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressQ96E93.
BgeeQ96E93.
CleanExHS_KLRG1.
HS_MAFA.
GenevestigatorQ96E93.

Family and domain databases

InterProIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
Gene3DG3DSA:3.10.100.10. C-type_lectin-like. 1 hit.
KOK10076.
PfamPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMSSF56436. C-type_lectin_fold. 1 hit.
PROSITEPS00615. C_TYPE_LECTIN_1. False negative.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio38696.
SOURCESearch...

Entry information

Entry nameKLRG1_HUMAN
AccessionPrimary (citable) accession number: Q96E93
Secondary accession number(s): B7ZAM2, O43198, O75613
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 1, 2001
Last modified: January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families