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Q96E11 (RRFM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome-recycling factor, mitochondrial

Short name=RRF
Alternative name(s):
Ribosome-releasing factor, mitochondrial
Gene names
Name:MRRF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the RRF family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransit peptide
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processribosome disassembly

Inferred from direct assay PubMed 19716793. Source: UniProtKB

translation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96E11-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96E11-2)

The sequence of this isoform differs from the canonical sequence as follows:
     185-201: VTREHREMLVKLAKQNT → SAKWPMTQWQNWTGIWQ
     202-262: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q96E11-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
Note: Derived from EST data. No experimental confirmation available.
Isoform 4 (identifier: Q96E11-4)

The sequence of this isoform differs from the canonical sequence as follows:
     154-262: CTAAAIKAIR...LAVKTKELLG → ELAWIRSLRVPGIRSHLYHQLAV
Note: Derived from EST data. No experimental confirmation available.
Isoform 5 (identifier: Q96E11-5)

The sequence of this isoform differs from the canonical sequence as follows:
     154-158: CTAAA → VRWPC
     159-262: Missing.
Note: Derived from EST data. No experimental confirmation available.
Isoform 6 (identifier: Q96E11-6)

The sequence of this isoform differs from the canonical sequence as follows:
     154-168: CTAAAIKAIRESGMN → TMKQIKQYFNGPNVA
     169-262: Missing.
Note: Derived from EST data. No experimental confirmation available.
Isoform 7 (identifier: Q96E11-7)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     154-168: CTAAAIKAIRESGMN → TMKQIKQYFNGPNVA
     169-262: Missing.
Note: Derived from EST data. No experimental confirmation available.
Isoform 8 (identifier: Q96E11-8)

The sequence of this isoform differs from the canonical sequence as follows:
     62-113: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5555Mitochondrion Potential
Chain56 – 262207Ribosome-recycling factor, mitochondrial
PRO_0000031080

Natural variations

Alternative sequence1 – 4444Missing in isoform 3 and isoform 7.
VSP_013483
Alternative sequence62 – 11352Missing in isoform 8.
VSP_042019
Alternative sequence154 – 262109CTAAA…KELLG → ELAWIRSLRVPGIRSHLYHQ LAV in isoform 4.
VSP_013484
Alternative sequence154 – 16815CTAAA…ESGMN → TMKQIKQYFNGPNVA in isoform 6 and isoform 7.
VSP_013485
Alternative sequence154 – 1585CTAAA → VRWPC in isoform 5.
VSP_013486
Alternative sequence159 – 262104Missing in isoform 5.
VSP_013487
Alternative sequence169 – 26294Missing in isoform 6 and isoform 7.
VSP_013488
Alternative sequence185 – 20117VTREH…AKQNT → SAKWPMTQWQNWTGIWQ in isoform 2.
VSP_013489
Alternative sequence202 – 26261Missing in isoform 2.
VSP_013490
Natural variant2161M → V.
Corresponds to variant rs2297483 [ dbSNP | Ensembl ].
VAR_051885

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: A4343465B5C9F5DE

FASTA26229,277
        10         20         30         40         50         60 
MALGLKCFRM VHPTFRNYLA ASIRPVSEVT LKTVHERQHG HRQYMAYSAV PVRHFATKKA 

        70         80         90        100        110        120 
KAKGKGQSQT RVNINAALVE DIINLEEVNE EMKSVIEALK DNFNKTLNIR TSPGSLDKIA 

       130        140        150        160        170        180 
VVTADGKLAL NQISQISMKS PQLILVNMAS FPECTAAAIK AIRESGMNLN PEVEGTLIRV 

       190        200        210        220        230        240 
PIPQVTREHR EMLVKLAKQN TNKAKDSLRK VRTNSMNKLK KSKDTVSEDT IRLIEKQISQ 

       250        260 
MADDTVAELD RHLAVKTKEL LG 

« Hide

Isoform 2 [UniParc].

Checksum: 9965A98B2AD33AC5
Show »

FASTA20122,450
Isoform 3 [UniParc].

Checksum: D2393AB713FD04FB
Show »

FASTA21824,115
Isoform 4 [UniParc].

Checksum: FEDFE00D670084CF
Show »

FASTA17619,742
Isoform 5 [UniParc].

Checksum: 395151EF011182AB
Show »

FASTA15817,687
Isoform 6 [UniParc].

Checksum: 51468DB2F03B79FB
Show »

FASTA16818,766
Isoform 7 [UniParc].

Checksum: C8C91CFD6BF33A9F
Show »

FASTA12413,604
Isoform 8 [UniParc].

Checksum: F8CEBCB81AAE3D4E
Show »

FASTA21023,511

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 5; 6 AND 8).
Tissue: Brain, Lung, Placenta, Prostate, Skeletal muscle and Trachea.
[2]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1; 3; 4; 5; 6 AND 7).
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Lung.
[5]"Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor."
Zhang Y., Spremulli L.L.
Biochim. Biophys. Acta 1443:245-250(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[6]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK291603 mRNA. Translation: BAF84292.1.
AK298046 mRNA. Translation: BAH12711.1.
AK291809 mRNA. Translation: BAF84498.1.
AK292790 mRNA. Translation: BAF85479.1.
AK300718 mRNA. Translation: BAH13333.1.
AK316266 mRNA. Translation: BAH14637.1.
AL162424 Genomic DNA. Translation: CAI14707.1.
AL162424 Genomic DNA. Translation: CAI14708.1.
AL162424 Genomic DNA. Translation: CAI14711.1.
AL162424 Genomic DNA. Translation: CAI14712.1.
AL162424 Genomic DNA. Translation: CAI14713.1.
CH471090 Genomic DNA. Translation: EAW87525.1.
BC013049 mRNA. Translation: AAH13049.1.
BC053604 mRNA. Translation: AAH53604.1.
CCDSCCDS48013.1. [Q96E11-2]
CCDS55336.1. [Q96E11-8]
CCDS6840.1. [Q96E11-1]
RefSeqNP_001166983.1. NM_001173512.1. [Q96E11-8]
NP_620132.1. NM_138777.3. [Q96E11-1]
NP_954646.1. NM_199177.2. [Q96E11-2]
UniGeneHs.368011.

3D structure databases

ProteinModelPortalQ96E11.
SMRQ96E11. Positions 86-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124944. 15 interactions.
IntActQ96E11. 2 interactions.
MINTMINT-3052093.

PTM databases

PhosphoSiteQ96E11.

Polymorphism databases

DMDM62901043.

Proteomic databases

MaxQBQ96E11.
PaxDbQ96E11.
PRIDEQ96E11.

Protocols and materials databases

DNASU92399.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000297908; ENSP00000297908; ENSG00000148187. [Q96E11-8]
ENST00000344641; ENSP00000343867; ENSG00000148187. [Q96E11-1]
ENST00000373723; ENSP00000362828; ENSG00000148187. [Q96E11-2]
ENST00000373729; ENSP00000362834; ENSG00000148187. [Q96E11-3]
ENST00000373730; ENSP00000362835; ENSG00000148187. [Q96E11-4]
ENST00000394315; ENSP00000377850; ENSG00000148187. [Q96E11-2]
ENST00000467864; ENSP00000433288; ENSG00000148187. [Q96E11-4]
ENST00000470366; ENSP00000434807; ENSG00000148187. [Q96E11-6]
ENST00000489572; ENSP00000436360; ENSG00000148187. [Q96E11-6]
ENST00000546115; ENSP00000445588; ENSG00000148187. [Q96E11-6]
GeneID92399.
KEGGhsa:92399.
UCSCuc010mwa.3. human. [Q96E11-1]
uc011lyq.2. human. [Q96E11-5]
uc011lyr.2. human. [Q96E11-8]
uc022bmy.1. human. [Q96E11-2]

Organism-specific databases

CTD92399.
GeneCardsGC09P125026.
HGNCHGNC:7234. MRRF.
HPAHPA047177.
HPA049987.
MIM604602. gene.
neXtProtNX_Q96E11.
PharmGKBPA31029.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0233.
HOVERGENHBG061516.
InParanoidQ96E11.
KOK02838.
OMAYRRITDN.
OrthoDBEOG7G4QGH.
PhylomeDBQ96E11.
TreeFamTF323691.

Gene expression databases

BgeeQ96E11.
GenevestigatorQ96E11.

Family and domain databases

Gene3D3.30.1360.40. 1 hit.
InterProIPR024946. Arg_repress_C-like.
IPR002661. Ribosome_recyc_fac.
IPR023584. Ribosome_recyc_fac_dom.
[Graphical view]
PANTHERPTHR20982. PTHR20982. 1 hit.
PfamPF01765. RRF. 1 hit.
[Graphical view]
SUPFAMSSF55194. SSF55194. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi92399.
NextBio77750.
PROQ96E11.
SOURCESearch...

Entry information

Entry nameRRFM_HUMAN
AccessionPrimary (citable) accession number: Q96E11
Secondary accession number(s): A8K6D8 expand/collapse secondary AC list , A8K6Z4, B7Z4X5, B7Z6P7, Q5RKT1, Q5T7T0, Q5T7T1, Q5T7T2, Q5T7T3, Q5T7T4, Q5T7T5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM