Q96E11 (RRFM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome-recycling factor, mitochondrial
Short name=RRF

Alternative name(s):
Ribosome-releasing factor, mitochondrial

Gene names

Name:

MRRF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the RRF family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transit peptide
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ribosome disassembly Inferred from direct assay PubMed 19716793. Source: UniProtKB translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96E11-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96E11-2)

The sequence of this isoform differs from the canonical sequence as follows:
     185-201: VTREHREMLVKLAKQNT → SAKWPMTQWQNWTGIWQ
     202-262: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q96E11-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Note: Derived from EST data. No experimental confirmation available.

Isoform 4 (identifier: Q96E11-4)

The sequence of this isoform differs from the canonical sequence as follows:
     154-262: CTAAAIKAIR...LAVKTKELLG → ELAWIRSLRVPGIRSHLYHQLAV

Note: Derived from EST data. No experimental confirmation available.

Isoform 5 (identifier: Q96E11-5)

The sequence of this isoform differs from the canonical sequence as follows:
     154-158: CTAAA → VRWPC
     159-262: Missing.

Note: Derived from EST data. No experimental confirmation available.

Isoform 6 (identifier: Q96E11-6)

The sequence of this isoform differs from the canonical sequence as follows:
     154-168: CTAAAIKAIRESGMN → TMKQIKQYFNGPNVA
     169-262: Missing.

Note: Derived from EST data. No experimental confirmation available.

Isoform 7 (identifier: Q96E11-7)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     154-168: CTAAAIKAIRESGMN → TMKQIKQYFNGPNVA
     169-262: Missing.

Note: Derived from EST data. No experimental confirmation available.

Isoform 8 (identifier: Q96E11-8)

The sequence of this isoform differs from the canonical sequence as follows:
     62-113: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 55	55	Mitochondrion Potential
	Chain	56 – 262	207	Ribosome-recycling factor, mitochondrial		PRO_0000031080
Natural variations
	Alternative sequence	1 – 44	44	Missing in isoform 3 and isoform 7.		VSP_013483
	Alternative sequence	62 – 113	52	Missing in isoform 8.		VSP_042019
	Alternative sequence	154 – 262	109	CTAAA…KELLG → ELAWIRSLRVPGIRSHLYHQ LAV in isoform 4.		VSP_013484
	Alternative sequence	154 – 168	15	CTAAA…ESGMN → TMKQIKQYFNGPNVA in isoform 6 and isoform 7.		VSP_013485
	Alternative sequence	154 – 158	5	CTAAA → VRWPC in isoform 5.		VSP_013486
	Alternative sequence	159 – 262	104	Missing in isoform 5.		VSP_013487
	Alternative sequence	169 – 262	94	Missing in isoform 6 and isoform 7.		VSP_013488
	Alternative sequence	185 – 201	17	VTREH…AKQNT → SAKWPMTQWQNWTGIWQ in isoform 2.		VSP_013489
	Alternative sequence	202 – 262	61	Missing in isoform 2.		VSP_013490
	Natural variant	216	1	M → V. Corresponds to variant rs2297483 [ dbSNP | Ensembl ].		VAR_051885

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A4343465B5C9F5DE	FASTA	262	29,277
	10 20 30 40 50 60 MALGLKCFRM VHPTFRNYLA ASIRPVSEVT LKTVHERQHG HRQYMAYSAV PVRHFATKKA 70 80 90 100 110 120 KAKGKGQSQT RVNINAALVE DIINLEEVNE EMKSVIEALK DNFNKTLNIR TSPGSLDKIA 130 140 150 160 170 180 VVTADGKLAL NQISQISMKS PQLILVNMAS FPECTAAAIK AIRESGMNLN PEVEGTLIRV 190 200 210 220 230 240 PIPQVTREHR EMLVKLAKQN TNKAKDSLRK VRTNSMNKLK KSKDTVSEDT IRLIEKQISQ 250 260 MADDTVAELD RHLAVKTKEL LG
	Isoform 2 [UniParc]. Checksum: 9965A98B2AD33AC5	FASTA	201	22,450
	10 20 30 40 50 60 MALGLKCFRM VHPTFRNYLA ASIRPVSEVT LKTVHERQHG HRQYMAYSAV PVRHFATKKA 70 80 90 100 110 120 KAKGKGQSQT RVNINAALVE DIINLEEVNE EMKSVIEALK DNFNKTLNIR TSPGSLDKIA 130 140 150 160 170 180 VVTADGKLAL NQISQISMKS PQLILVNMAS FPECTAAAIK AIRESGMNLN PEVEGTLIRV 190 200 PIPQSAKWPM TQWQNWTGIW Q
	Isoform 3 [UniParc]. Checksum: D2393AB713FD04FB	FASTA	218	24,115
	10 20 30 40 50 60 MAYSAVPVRH FATKKAKAKG KGQSQTRVNI NAALVEDIIN LEEVNEEMKS VIEALKDNFN 70 80 90 100 110 120 KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPEC TAAAIKAIRE 130 140 150 160 170 180 SGMNLNPEVE GTLIRVPIPQ VTREHREMLV KLAKQNTNKA KDSLRKVRTN SMNKLKKSKD 190 200 210 TVSEDTIRLI EKQISQMADD TVAELDRHLA VKTKELLG
	Isoform 4 [UniParc]. Checksum: FEDFE00D670084CF	FASTA	176	19,742
	10 20 30 40 50 60 MALGLKCFRM VHPTFRNYLA ASIRPVSEVT LKTVHERQHG HRQYMAYSAV PVRHFATKKA 70 80 90 100 110 120 KAKGKGQSQT RVNINAALVE DIINLEEVNE EMKSVIEALK DNFNKTLNIR TSPGSLDKIA 130 140 150 160 170 VVTADGKLAL NQISQISMKS PQLILVNMAS FPEELAWIRS LRVPGIRSHL YHQLAV
	Isoform 5 [UniParc]. Checksum: 395151EF011182AB	FASTA	158	17,687
	10 20 30 40 50 60 MALGLKCFRM VHPTFRNYLA ASIRPVSEVT LKTVHERQHG HRQYMAYSAV PVRHFATKKA 70 80 90 100 110 120 KAKGKGQSQT RVNINAALVE DIINLEEVNE EMKSVIEALK DNFNKTLNIR TSPGSLDKIA 130 140 150 VVTADGKLAL NQISQISMKS PQLILVNMAS FPEVRWPC
	Isoform 6 [UniParc]. Checksum: 51468DB2F03B79FB	FASTA	168	18,766
	10 20 30 40 50 60 MALGLKCFRM VHPTFRNYLA ASIRPVSEVT LKTVHERQHG HRQYMAYSAV PVRHFATKKA 70 80 90 100 110 120 KAKGKGQSQT RVNINAALVE DIINLEEVNE EMKSVIEALK DNFNKTLNIR TSPGSLDKIA 130 140 150 160 VVTADGKLAL NQISQISMKS PQLILVNMAS FPETMKQIKQ YFNGPNVA
	Isoform 7 [UniParc]. Checksum: C8C91CFD6BF33A9F	FASTA	124	13,604
	10 20 30 40 50 60 MAYSAVPVRH FATKKAKAKG KGQSQTRVNI NAALVEDIIN LEEVNEEMKS VIEALKDNFN 70 80 90 100 110 120 KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPET MKQIKQYFNG PNVA
	Isoform 8 [UniParc]. Checksum: F8CEBCB81AAE3D4E	FASTA	210	23,511
	10 20 30 40 50 60 MALGLKCFRM VHPTFRNYLA ASIRPVSEVT LKTVHERQHG HRQYMAYSAV PVRHFATKKA 70 80 90 100 110 120 KGSLDKIAVV TADGKLALNQ ISQISMKSPQ LILVNMASFP ECTAAAIKAI RESGMNLNPE 130 140 150 160 170 180 VEGTLIRVPI PQVTREHREM LVKLAKQNTN KAKDSLRKVR TNSMNKLKKS KDTVSEDTIR 190 200 210 LIEKQISQMA DDTVAELDRH LAVKTKELLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 5; 6 AND 8). Tissue: Brain, Lung, Placenta, Prostate, Skeletal muscle and Trachea.
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1; 3; 4; 5; 6 AND 7).
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung.
[5]	"Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor." Zhang Y., Spremulli L.L. Biochim. Biophys. Acta 1443:245-250(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK291603 mRNA. Translation: BAF84292.1. AK298046 mRNA. Translation: BAH12711.1. AK291809 mRNA. Translation: BAF84498.1. AK292790 mRNA. Translation: BAF85479.1. AK300718 mRNA. Translation: BAH13333.1. AK316266 mRNA. Translation: BAH14637.1. AL162424 Genomic DNA. Translation: CAI14707.1. AL162424 Genomic DNA. Translation: CAI14708.1. AL162424 Genomic DNA. Translation: CAI14711.1. AL162424 Genomic DNA. Translation: CAI14712.1. AL162424 Genomic DNA. Translation: CAI14713.1. CH471090 Genomic DNA. Translation: EAW87525.1. BC013049 mRNA. Translation: AAH13049.1. BC053604 mRNA. Translation: AAH53604.1.
IPI	IPI00030596. IPI00061108. IPI00412392. IPI00514034. IPI00555800. IPI00556454. IPI00556600.
RefSeq	NP_001166983.1. NM_001173512.1. NP_620132.1. NM_138777.3. NP_954646.1. NM_199177.2.
UniGene	Hs.368011.
3D structure databases
ProteinModelPortal	Q96E11.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96E11. 1 interaction.
PTM databases
PhosphoSite	Q96E11.
Polymorphism databases
DMDM	62901043.
Proteomic databases
PaxDb	Q96E11.
PRIDE	Q96E11.
Protocols and materials databases
DNASU	92399.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000297908; ENSP00000297908; ENSG00000148187. ENST00000344641; ENSP00000343867; ENSG00000148187. ENST00000373723; ENSP00000362828; ENSG00000148187. ENST00000373724; ENSP00000362829; ENSG00000148187. ENST00000373727; ENSP00000362832; ENSG00000148187. ENST00000373728; ENSP00000362833; ENSG00000148187. ENST00000373729; ENSP00000362834; ENSG00000148187. ENST00000373730; ENSP00000362835; ENSG00000148187. ENST00000394315; ENSP00000377850; ENSG00000148187. ENST00000441707; ENSP00000395072; ENSG00000148187. ENST00000467864; ENSP00000433288; ENSG00000148187. ENST00000470366; ENSP00000434807; ENSG00000148187. ENST00000489572; ENSP00000436360; ENSG00000148187. ENST00000546115; ENSP00000445588; ENSG00000148187.
GeneID	92399.
KEGG	hsa:92399.
UCSC	uc010mwa.3. human. uc011lyq.2. human. uc022bmy.1. human.
Organism-specific databases
CTD	92399.
GeneCards	GC09P125026.
HGNC	HGNC:7234. MRRF.
MIM	604602. gene.
neXtProt	NX_Q96E11.
PharmGKB	PA31029.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0233.
HOVERGEN	HBG061516.
InParanoid	Q96E11.
KO	K02838.
OMA	VMVEYYG.
OrthoDB	EOG4QRH4V.
PhylomeDB	Q96E11.
Gene expression databases
Bgee	Q96E11.
Genevestigator	Q96E11.
GermOnline	ENSG00000148187. Homo sapiens.
Family and domain databases
Gene3D	3.30.1360.40. 1 hit.
InterPro	IPR024946. Arg_repress_C-like. IPR002661. Ribosome_recyc_fac. IPR023584. Ribosome_recyc_fac_dom. [Graphical view]
PANTHER	PTHR20982. PTHR20982. 1 hit.
Pfam	PF01765. RRF. 1 hit. [Graphical view]
SUPFAM	SSF55194. Ribosome_recyc_fac. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	92399.
NextBio	77750.
SOURCE	Search...

Entry information

Entry name

RRFM_HUMAN

Accession

Primary (citable) accession number: Q96E11
Secondary accession number(s): A8K6D8 , A8K6Z4, B7Z4X5, B7Z6P7, Q5RKT1, Q5T7T0, Q5T7T1, Q5T7T2, Q5T7T3, Q5T7T4, Q5T7T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.