Q96DX5 (ASB9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ankyrin repeat and SOCS box protein 9
Short name=ASB-9

Gene names

Name:

ASB9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes at least two forms of creatine kinase, CKB and CKMT1A. Ref.5 Ref.6
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Mitochondrion Ref.5.
Tissue specificity	Predominantly expressed in testis, kidney, and liver. Ref.5
Domain	The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes By similarity.
Sequence similarities	Contains 6 ANK repeats. Contains 1 SOCS box domain.
Sequence caution	The sequence CAB45706.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro positive regulation of protein catabolic process Inferred from direct assay PubMed 17148442. Source: MGI protein ubiquitination Inferred from direct assay PubMed 17148442. Source: MGI
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CKB	P12277	3	EBI-745641,EBI-357706
HIF1AN	Q9NWT6	3	EBI-745641,EBI-745632

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96DX5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96DX5-2)

Also known as: ASB9deltaSOCS;

The sequence of this isoform differs from the canonical sequence as follows:
     255-294: PPSLMQLCRLRIRKCFGIQQHHKITKLVLPEDLKQFLLHL → ASLPKPKP

Note: Does not interact with the Elongin BC complex, likely to be a negative regulator of isoform 1.

Isoform 3 (identifier: Q96DX5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     145-154: Missing.
     255-294: PPSLMQLCRLRIRKCFGIQQHHKITKLVLPEDLKQFLLHL → ASLPKPKP

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 294

294

Ankyrin repeat and SOCS box protein 9

PRO_0000066940

Regions

Repeat

35 – 64

30

ANK 1

Repeat

68 – 97

30

ANK 2

Repeat

101 – 130

30

ANK 3

Repeat

133 – 162

30

ANK 4

Repeat

166 – 195

30

ANK 5

Repeat

198 – 227

30

ANK 6

Domain

240 – 294

55

SOCS box

Sites

Site

103

1

Essential for binding to CKB

Site

107

1

Essential for binding to CKB

Natural variations

Alternative sequence

145 – 154

10

Missing in isoform 3.

VSP_043158

Alternative sequence

255 – 294

40

PPSLM…FLLHL → ASLPKPKP in isoform 2 and isoform 3.

VSP_000271

Experimental info

Sequence conflict

192

1

D → V in CAB45706. Ref.2

Sequence conflict

237

1

E → G in CAB45706. Ref.2

Secondary structure

1

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294

Helix Strand Turn

Details...

Beta strand

21 – 23

3

Beta strand

31 – 33

3

Helix

39 – 45

7

Helix

49 – 57

9

Helix

72 – 78

7

Helix

82 – 90

9

Helix

105 – 112

8

Helix

115 – 123

9

Helix

137 – 144

8

Helix

147 – 155

9

Turn

165 – 167

3

Helix

170 – 176

7

Helix

180 – 188

9

Helix

202 – 208

7

Helix

212 – 220

9

Helix

235 – 238

4

Helix

244 – 252

9

Helix

258 – 267

10

Helix

275 – 280

6

Beta strand

281 – 283

3

Helix

285 – 291

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A1784DCFC294D85A	FASTA	294	31,858
	10 20 30 40 50 60 MDGKQGGMDG SKPAGPRDFP GIRLLSNPLM GDAVSDWSPM HEAAIHGHQL SLRNLISQGW 70 80 90 100 110 120 AVNIITADHV SPLHEACLGG HLSCVKILLK HGAQVNGVTA DWHTPLFNAC VSGSWDCVNL 130 140 150 160 170 180 LLQHGASVQP ESDLASPIHE AARRGHVECV NSLIAYGGNI DHKISHLGTP LYLACENQQR 190 200 210 220 230 240 ACVKKLLESG ADVNQGKGQD SPLHAVARTA SEELACLLMD FGADTQAKNA EGKRPVELVP 250 260 270 280 290 PESPLAQLFL EREGPPSLMQ LCRLRIRKCF GIQQHHKITK LVLPEDLKQF LLHL
	Isoform 2 (ASB9deltaSOCS) [UniParc]. Checksum: 9354CAD758EC399F	FASTA	262	27,902
	10 20 30 40 50 60 MDGKQGGMDG SKPAGPRDFP GIRLLSNPLM GDAVSDWSPM HEAAIHGHQL SLRNLISQGW 70 80 90 100 110 120 AVNIITADHV SPLHEACLGG HLSCVKILLK HGAQVNGVTA DWHTPLFNAC VSGSWDCVNL 130 140 150 160 170 180 LLQHGASVQP ESDLASPIHE AARRGHVECV NSLIAYGGNI DHKISHLGTP LYLACENQQR 190 200 210 220 230 240 ACVKKLLESG ADVNQGKGQD SPLHAVARTA SEELACLLMD FGADTQAKNA EGKRPVELVP 250 260 PESPLAQLFL EREGASLPKP KP
	Isoform 3 [UniParc]. Checksum: 6FEE897C6B1FF98C	FASTA	252	26,849
	10 20 30 40 50 60 MDGKQGGMDG SKPAGPRDFP GIRLLSNPLM GDAVSDWSPM HEAAIHGHQL SLRNLISQGW 70 80 90 100 110 120 AVNIITADHV SPLHEACLGG HLSCVKILLK HGAQVNGVTA DWHTPLFNAC VSGSWDCVNL 130 140 150 160 170 180 LLQHGASVQP ESDLASPIHE AARRAYGGNI DHKISHLGTP LYLACENQQR ACVKKLLESG 190 200 210 220 230 240 ADVNQGKGQD SPLHAVARTA SEELACLLMD FGADTQAKNA EGKRPVELVP PESPLAQLFL 250 EREGASLPKP KP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Cervix and Skin.
[5]	"ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function." Kwon S., Kim D., Rhee J.W., Park J.A., Kim D.W., Kim D.S., Lee Y., Kwon H.J. BMC Biol. 8:23-23(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"Crystal structure of human ASB9-2 and substrate-recognition of CKB." Fei X., Gu X., Fan S., Yang Z., Li F., Zhang C., Gong W., Mao Y., Ji C. Protein J. 31:275-284(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) (ISOFORM 2), FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK000643 mRNA. Translation: BAA91302.1.
AK292270 mRNA. Translation: BAF84959.1.
AL080091 mRNA. Translation: CAB45706.2. Different initiation.
CH471074 Genomic DNA. Translation: EAW98871.1.
CH471074 Genomic DNA. Translation: EAW98874.1.
BC001244 mRNA. Translation: AAH01244.1.
BC013172 mRNA. Translation: AAH13172.1.

IPI

IPI00179183.
IPI00216580.
IPI00643086.

RefSeq

NP_001026909.1. NM_001031739.2.
NP_001162002.1. NM_001168530.1.
NP_001162003.1. NM_001168531.1.
NP_076992.1. NM_024087.2.

UniGene

Hs.19404.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3D9H	X-ray	2.20	A	1-254	[»]
3ZKJ	X-ray	2.58	A/D	35-294	[»]

ProteinModelPortal

Q96DX5.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96DX5. 4 interactions.

MINT

MINT-5003430.

STRING

9606.ENSP00000369855.

PTM databases

PhosphoSite

Q96DX5.

Polymorphism databases

DMDM

29839756.

2D gel databases

REPRODUCTION-2DPAGE

IPI00179183.

Proteomic databases

PaxDb

Q96DX5.

PRIDE

Q96DX5.

Protocols and materials databases

DNASU

140462.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000380483; ENSP00000369850; ENSG00000102048.
ENST00000380485; ENSP00000369852; ENSG00000102048.
ENST00000380488; ENSP00000369855; ENSG00000102048.
ENST00000546332; ENSP00000438943; ENSG00000102048.

GeneID

140462.

KEGG

hsa:140462.

UCSC

uc004cwk.3. human.
uc004cwl.3. human.

Organism-specific databases

CTD

140462.

GeneCards

GC0XM015252.

HGNC

HGNC:17184. ASB9.

HPA

CAB004997.
HPA003014.
HPA003060.

MIM

300890. gene.

neXtProt

NX_Q96DX5.

PharmGKB

PA25037.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0666.

HOGENOM

HOG000231513.

HOVERGEN

HBG001376.

InParanoid

Q96DX5.

KO

K10331.

OMA

SQGWPVN.

OrthoDB

EOG4TB4BS.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

UniPathway

UPA00143.

Gene expression databases

Bgee

Q96DX5.

CleanEx

HS_ASB9.

Genevestigator

Q96DX5.

GermOnline

ENSG00000102048. Homo sapiens.

Family and domain databases

Gene3D

1.25.40.20. 2 hits.

InterPro

IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001496. SOCS_C.
[Graphical view]

Pfam

PF12796. Ank_2. 2 hits.
PF07525. SOCS_box. 1 hit.
[Graphical view]

SMART

SM00248. ANK. 6 hits.
SM00969. SOCS_box. 1 hit.
[Graphical view]

SUPFAM

SSF48403. ANK. 1 hit.

PROSITE

PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50225. SOCS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q96DX5.

GenomeRNAi

140462.

NextBio

84125.

SOURCE

Search...

Entry information

Entry name

ASB9_HUMAN

Accession

Primary (citable) accession number: Q96DX5
Secondary accession number(s): A8K8A5 , Q9BVF5, Q9NWS5, Q9Y4T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.