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Q96DR7 (ARHGQ_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho guanine nucleotide exchange factor 26
Alternative name(s):
SH3 domain-containing guanine exchange factor
Gene names
Name:ARHGEF26
Synonyms:SGEF
ORF Names:HMFN1864
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length871 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates RhoG GTPase by promoting the exchange of GDP by GTP. Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB, which induces cytoskeleton rearrangements and promotes bacterial entry. Ref.2 Ref.8 Ref.9

Subunit structure

Interacts with ICAM1 and RHOG. Ref.2 Ref.9

Subcellular location

Cell projectionruffle Ref.2 Ref.9.

Tissue specificity

Isoform 1 is broadly expressed, with highest levels in liver (at protein level). Certain mRNA species appear to be specifically expressed in prostate and liver. Ref.1 Ref.2

Induction

Certain mRNA species appear to be up-regulated by androgens in prostate cancer cells. Ref.1

Sequence similarities

Contains 1 DH (DBL-homology) domain.

Contains 1 PH domain.

Contains 1 SH3 domain.

Sequence caution

The sequence AAH16628.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAL27002.1 differs from that shown. Reason: Intron retention.

The sequence BAB14159.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAB14292.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Cellular componentCell projection
   Coding sequence diversityAlternative promoter usage
Alternative splicing
Polymorphism
   DomainSH3 domain
   Molecular functionGuanine-nucleotide releasing factor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentruffle

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRho guanyl-nucleotide exchange factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96DR7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96DR7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     428-446: GLSQTVSQEERKRQEAIFE → VILIVGFMEMKDGRLRGGK
     447-871: Missing.
Isoform 3 (identifier: Q96DR7-4)

The sequence of this isoform differs from the canonical sequence as follows:
     790-871: SLTQVEIVRS...GRLLGLETNV → CG
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 871871Rho guanine nucleotide exchange factor 26
PRO_0000322568

Regions

Domain439 – 623185DH
Domain655 – 782128PH
Domain789 – 85062SH3
Compositional bias106 – 17368Pro-rich

Amino acid modifications

Modified residue221Phosphoserine Ref.11
Modified residue3921Phosphoserine Ref.10 Ref.11

Natural variations

Alternative sequence428 – 44619GLSQT…EAIFE → VILIVGFMEMKDGRLRGGK in isoform 2.
VSP_031934
Alternative sequence447 – 871425Missing in isoform 2.
VSP_031935
Alternative sequence790 – 87182SLTQV…LETNV → CG in isoform 3.
VSP_045570
Natural variant291V → L. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.11
Corresponds to variant rs12493885 [ dbSNP | Ensembl ].
VAR_039425
Natural variant601L → P. Ref.4
Corresponds to variant rs12497267 [ dbSNP | Ensembl ].
VAR_058205
Natural variant2031F → S. Ref.4
Corresponds to variant rs13096373 [ dbSNP | Ensembl ].
VAR_039426

Experimental info

Mutagenesis8261W → R: Fails to localize at sites of membrane ruffling. Ref.2
Sequence conflict601L → S in AAL27001. Ref.1
Sequence conflict601L → S in AAS59842. Ref.2
Sequence conflict601L → S in BAG53860. Ref.3
Sequence conflict601L → S in AAH78655. Ref.6
Sequence conflict6871N → K in AAH16628. Ref.6
Sequence conflict7071S → T in AAH78655. Ref.6
Sequence conflict7811S → R in AAL27001. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 11, 2011. Version 4.
Checksum: E6EB57BAE7DD224B

FASTA87197,346
        10         20         30         40         50         60 
MDGESEVDFS SNSITPLWRR RSIPQPHQVL GRSKPRPQSY QSPNGLLITD FPVEDGGTLL 

        70         80         90        100        110        120 
AAQIPAQVPT ASDSRTVHRS PLLLGAQRRA VANGGTASPE YRAASPRLRR PKSPKLPKAV 

       130        140        150        160        170        180 
PGGSPKSPAN GAVTLPAPPP PPVLRPPRTP NAPAPCTPEE DLTGLTASPV PSPTANGLAA 

       190        200        210        220        230        240 
NNDSPGSGSQ SGRKAKDPER GLFPGPQKSS SEQKLPLQRL PSQENELLEN PSVVLSTNSP 

       250        260        270        280        290        300 
AALKVGKQQI IPKSLASEIK ISKSNNQNVE PHKRLLKVRS MVEGLGGPLG HAGEESEVDN 

       310        320        330        340        350        360 
DVDSPGSLRR GLRSTSYRRA VVSGFDFDSP TSSKKKNRMS QPVLKVVMED KEKFSSLGRI 

       370        380        390        400        410        420 
KKKMLKGQGT FDGEENAVLY QNYKEKALDI DSDEESEPKE QKSDEKIVIH HKPLRSTWSQ 

       430        440        450        460        470        480 
LSAVKRKGLS QTVSQEERKR QEAIFEVISS EHSYLLSLEI LIRMFKNSKE LSDTMTKTER 

       490        500        510        520        530        540 
HHLFSNITDV CEASKKFFIE LEARHQNNIF IDDISDIVEK HTASTFDPYV KYCTNEVYQQ 

       550        560        570        580        590        600 
RTLQKLLATN PSFKEVLSRI ESHEDCRNLP MISFLILPMQ RVTRLPLLMD TICQKTPKDS 

       610        620        630        640        650        660 
PKYEVCKRAL KEVSKLVRLC NEGARKMERT EMMYTINSQL EFKIKPFPLV SSSRWLVKRG 

       670        680        690        700        710        720 
ELTAYVEDTV LFSRRTSKQQ VYFFLFNDVL IITKKKSEES YNVNDYSLRD QLLVESCDNE 

       730        740        750        760        770        780 
ELNSSPGKNS STMLYSRQSS ASHLFTLTVL SNHANEKVEM LLGAETQSER ARWITALGHS 

       790        800        810        820        830        840 
SGKPPADRTS LTQVEIVRSF TAKQPDELSL QVADVVLIYQ RVSDGWYEGE RLRDGERGWF 

       850        860        870 
PMECAKEITC QATIDKNVER MGRLLGLETN V 

« Hide

Isoform 2 [UniParc].

Checksum: 4D112FF2EDBD3C26
Show »

FASTA44648,168
Isoform 3 [UniParc].

Checksum: 3722051098F4B67B
Show »

FASTA79188,134

References

« Hide 'large scale' references
[1]"Isolation of the novel human guanine nucleotide exchange factor Src homology 3 domain-containing guanine nucleotide exchange factor (SGEF) and of C-terminal SGEF, an N-terminally truncated form of SGEF, the expression of which is regulated by androgen in prostate cancer cells."
Qi H., Fournier A., Grenier J., Fillion C., Labrie Y., Labrie C.
Endocrinology 144:1742-1752(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INDUCTION, VARIANT LEU-29.
Tissue: Prostatic carcinoma.
[2]"SGEF, a RhoG guanine nucleotide exchange factor that stimulates macropinocytosis."
Ellerbroek S.M., Wennerberg K., Arthur W.T., Dunty J.M., Bowman D.R., DeMali K.A., Der C., Burridge K.
Mol. Biol. Cell 15:3309-3319(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH RHOG, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF TRP-826, VARIANT LEU-29.
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 402-871 (ISOFORM 1), VARIANT LEU-29.
[4]"Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas."
Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A.
Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS LEU-29; PRO-60 AND SER-203.
Tissue: Liver.
[5]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LEU-29.
Tissue: Prostate and Testis.
[7]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 661-871 (ISOFORM 1).
Tissue: Testis.
[8]"Differential activation and function of Rho GTPases during Salmonella-host cell interactions."
Patel J.C., Galan J.E.
J. Cell Biol. 175:453-463(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration."
van Buul J.D., Allingham M.J., Samson T., Meller J., Boulter E., Garcia-Mata R., Burridge K.
J. Cell Biol. 178:1279-1293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ICAM1, SUBCELLULAR LOCATION.
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[11]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22 AND SER-392, VARIANT [LARGE SCALE ANALYSIS] LEU-29, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF415175 mRNA. Translation: AAL27001.1.
AF415176 mRNA. Translation: AAL27002.1. Sequence problems.
AY552599 mRNA. Translation: AAS59842.1.
AK022655 mRNA. Translation: BAB14159.1. Different initiation.
AK022884 mRNA. Translation: BAB14292.1. Different initiation.
AK123040 mRNA. Translation: BAG53860.1.
AB073386 mRNA. Translation: BAD38637.1.
AC018452 Genomic DNA. No translation available.
FJ695204 Genomic DNA. No translation available.
BC016628 mRNA. Translation: AAH16628.1. Different initiation.
BC078655 mRNA. Translation: AAH78655.1.
AL117429 mRNA. Translation: CAB55918.1.
PIRT17228.
RefSeqNP_001238891.1. NM_001251962.1.
NP_001238892.1. NM_001251963.1.
NP_056410.3. NM_015595.3.
UniGeneHs.240845.

3D structure databases

ProteinModelPortalQ96DR7.
SMRQ96DR7. Positions 433-849.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ96DR7.

Polymorphism databases

DMDM317373555.

Proteomic databases

PaxDbQ96DR7.
PRIDEQ96DR7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000356448; ENSP00000348828; ENSG00000114790. [Q96DR7-1]
ENST00000465093; ENSP00000423418; ENSG00000114790. [Q96DR7-1]
ENST00000465817; ENSP00000423295; ENSG00000114790. [Q96DR7-3]
ENST00000496710; ENSP00000424446; ENSG00000114790. [Q96DR7-4]
GeneID26084.
KEGGhsa:26084.
UCSCuc011bog.1. human. [Q96DR7-1]

Organism-specific databases

CTD26084.
GeneCardsGC03P153839.
H-InvDBHIX0003789.
HGNCHGNC:24490. ARHGEF26.
HPAHPA017722.
neXtProtNX_Q96DR7.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5422.
HOVERGENHBG059167.
InParanoidQ96DR7.
KOK13744.
OMANSITPLW.
OrthoDBEOG73NG2P.
PhylomeDBQ96DR7.
TreeFamTF316357.

Gene expression databases

BgeeQ96DR7.
GenevestigatorQ96DR7.

Family and domain databases

Gene3D1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR000219. DH-domain.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi26084.
NextBio48000.
PROQ96DR7.

Entry information

Entry nameARHGQ_HUMAN
AccessionPrimary (citable) accession number: Q96DR7
Secondary accession number(s): B3KVP8 expand/collapse secondary AC list , E9PBD0, Q68CL1, Q6AZ96, Q6Q8Q8, Q96AW8, Q96DR6, Q9H9D7, Q9H9R2, Q9UFW5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 11, 2011
Last modified: April 16, 2014
This is version 90 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM