Q96DN2 (VWCE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
von Willebrand factor C and EGF domain-containing protein

Alternative name(s):
HBV X protein up-regulated gene 11 protein
HBxAg up-regulated gene 11 protein

Gene names

Name:	VWCE
Synonyms:	URG11

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	955 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be a regulatory element in the beta-catenin signaling pathway and a target for chemoprevention of hapatocellular carcinoma. Ref.5
Subcellular location	Secreted Potential. Note: According to Ref.5 is localized in the cytoplasm. Ref.5
Tissue specificity	Expressed in liver. Ref.5
Induction	By HBxAg. Ref.5
Sequence similarities	Contains 4 EGF-like domains. Contains 6 VWFC domains.
Sequence caution	The sequence AAO47606.1 differs from that shown. Reason: Frameshift at positions 210, 232, 745, 761, 769, 837 and 912.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	EGF-like domain Repeat Signal
Ligand	Calcium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96DN2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96DN2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     219-219: V → M
     220-955: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 955	934	von Willebrand factor C and EGF domain-containing protein		PRO_0000318580
Regions
	Domain	70 – 98	29	EGF-like 1
	Domain	142 – 180	39	EGF-like 2; calcium-binding Potential
	Domain	181 – 219	39	EGF-like 3; calcium-binding Potential
	Domain	220 – 262	43	EGF-like 4; calcium-binding Potential
	Domain	384 – 441	58	VWFC 1
	Domain	442 – 502	61	VWFC 2
	Domain	503 – 560	58	VWFC 3
	Domain	566 – 626	61	VWFC 4
	Domain	627 – 685	59	VWFC 5
	Domain	686 – 743	58	VWFC 6
	Compositional bias	285 – 288	4	Poly-Leu
	Compositional bias	835 – 872	38	Pro-rich
Amino acid modifications
	Glycosylation	462	1	N-linked (GlcNAc...) Potential
	Glycosylation	472	1	N-linked (GlcNAc...) Potential
	Disulfide bond	146 ↔ 155		By similarity
	Disulfide bond	151 ↔ 164		By similarity
	Disulfide bond	166 ↔ 179		By similarity
	Disulfide bond	185 ↔ 194		By similarity
	Disulfide bond	190 ↔ 203		By similarity
	Disulfide bond	205 ↔ 218		By similarity
	Disulfide bond	224 ↔ 237		By similarity
	Disulfide bond	233 ↔ 246		By similarity
	Disulfide bond	248 ↔ 261		By similarity
Natural variations
	Alternative sequence	219	1	V → M in isoform 2.		VSP_031230
	Alternative sequence	220 – 955	736	Missing in isoform 2.		VSP_031231
	Natural variant	842	1	P → R. Corresponds to variant rs3750982 [ dbSNP | Ensembl ].		VAR_038782
Experimental info
	Sequence conflict	134	1	T → A in BAB71219. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 24, 2009. Version 2. Checksum: B745618AD37E712B	FASTA	955	99,915
	10 20 30 40 50 60 MWAGLLLRAA CVALLLPGAP ARGYTGRKPP GHFAAERRRL GPHVCLSGFG SGCCPGWAPS 70 80 90 100 110 120 MGGGHCTLPL CSFGCGSGIC IAPNVCSCQD GEQGATCPET HGPCGEYGCD LTCNHGGCQE 130 140 150 160 170 180 VARVCPVGFS MTETAVGIRC TDIDECVTSS CEGHCVNTEG GFVCECGPGM QLSADRHSCQ 190 200 210 220 230 240 DTDECLGTPC QQRCKNSIGS YKCSCRTGFH LHGNRHSCVD VNECRRPLER RVCHHSCHNT 250 260 270 280 290 300 VGSFLCTCRP GFRLRADRVS CEAFPKAVLA PSAILQPRQH PSKMLLLLPE AGRPALSPGH 310 320 330 340 350 360 SPPSGAPGPP AGVRTTRLPS PTPRLPTSSP SAPVWLLSTL LATPVPTASL LGNLRPPSLL 370 380 390 400 410 420 QGEVMGTPSS PRGPESPRLA AGPSPCWHLG AMHESRSRWT EPGCSQCWCE DGKVTCEKVR 430 440 450 460 470 480 CEAACSHPIP SRDGGCCPSC TGCFHSGVVR AEGDVFSPPN ENCTVCVCLA GNVSCISPEC 490 500 510 520 530 540 PSGPCQTPPQ TDCCTCVPVR CYFHGRWYAD GAVFSGGGDE CTTCVCQNGE VECSFMPCPE 550 560 570 580 590 600 LACPREEWRL GPGQCCFTCQ EPTPSTGCSL DDNGVEFPIG QIWSPGDPCE LCICQADGSV 610 620 630 640 650 660 SCKRTDCVDS CPHPIRIPGQ CCPDCSAGCT YTGRIFYNNE TFPSVLDPCL SCICLLGSVA 670 680 690 700 710 720 CSPVDCPITC TYPFHPDGEC CPVCRDCNYE GRKVANGQVF TLDDEPCTRC TCQLGEVSCE 730 740 750 760 770 780 KVPCQRACAD PALLPGDCCS SCPDSLSPLE EKQGLSPHGN VAFSKAGRSL HGDTEAPVNC 790 800 810 820 830 840 SSCPGPPTAS PSRPVLHLLQ LLLRTNLMKT QTLPTSPAGA HGPHSLALGL TATFPGEPGA 850 860 870 880 890 900 SPRLSPGPST PPGAPTLPLA SPGAPQPPPV TPERSFSASG AQIVSRWPPL PGTLLTEASA 910 920 930 940 950 LSMMDPSPSK TPITLLGPRV LSPTTSRLST ALAATTHPGP QQPPVGASRG EESTM
	Isoform 2 [UniParc]. Checksum: B018610FE43DAE45	FASTA	219	22,790
	10 20 30 40 50 60 MWAGLLLRAA CVALLLPGAP ARGYTGRKPP GHFAAERRRL GPHVCLSGFG SGCCPGWAPS 70 80 90 100 110 120 MGGGHCTLPL CSFGCGSGIC IAPNVCSCQD GEQGATCPET HGPCGEYGCD LTCNHGGCQE 130 140 150 160 170 180 VARVCPVGFS MTETAVGIRC TDIDECVTSS CEGHCVNTEG GFVCECGPGM QLSADRHSCQ 190 200 210 DTDECLGTPC QQRCKNSIGS YKCSCRTGFH LHGNRHSCM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Teratocarcinoma.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lymph.
[4]	"Upregulated expression of a unique gene by hepatitis B x antigen promotes hepatocellular growth and tumorigenesis." Lian Z., Liu J., Li L., Li X., Tufan S.N.L., Clayton M., Wu M.-C., Wang H.-Y., Arbuthnot P., Kew M., Feitelson M.A. Neoplasia 5:229-244(2003) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-955 (ISOFORM 1).
[5]	"Enhanced cell survival of Hep3B cells by the hepatitis B x antigen effector, URG11, is associated with upregulation of beta-catenin." Lian Z., Liu J., Li L., Li X., Clayton M., Wu M.-C., Wang H.-Y., Arbuthnot P., Kew M., Fan D., Feitelson M.A. Hepatology 43:415-424(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY HBXAG, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK056571 mRNA. Translation: BAB71219.1. BC052564 mRNA. No translation available. BC077719 mRNA. No translation available. BC142624 mRNA. Translation: AAI42625.1. CH471076 Genomic DNA. Translation: EAW73932.1. AY205235 mRNA. Translation: AAO47606.1. Frameshift.
IPI	IPI00061007. IPI00884977.
RefSeq	NP_689931.2. NM_152718.2.
UniGene	Hs.60640.
3D structure databases
HSSP	HSSP built from PDB template 1WHF based on UniProtKB P00743.
ProteinModelPortal	Q96DN2.
SMR	Q96DN2. Positions 73-288.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96DN2. 1 interaction.
STRING	9606.ENSP00000334186.
PTM databases
PhosphoSite	Q96DN2.
Polymorphism databases
DMDM	269849477.
Proteomic databases
PaxDb	Q96DN2.
PRIDE	Q96DN2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000301770; ENSP00000301770; ENSG00000167992. ENST00000335613; ENSP00000334186; ENSG00000167992.
GeneID	220001.
KEGG	hsa:220001.
UCSC	uc001nra.3. human.
Organism-specific databases
CTD	220001.
GeneCards	GC11M061025.
H-InvDB	HIX0009684.
HGNC	HGNC:26487. VWCE.
HPA	HPA040401. HPA043921.
MIM	611115. gene.
neXtProt	NX_Q96DN2.
PharmGKB	PA143485669.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG240200.
HOGENOM	HOG000168460.
HOVERGEN	HBG067558.
InParanoid	Q96DN2.
OMA	APVNCSS.
OrthoDB	EOG490788.
PhylomeDB	Q96DN2.
Gene expression databases
ArrayExpress	Q96DN2.
Bgee	Q96DN2.
CleanEx	HS_VWCE.
Genevestigator	Q96DN2.
Family and domain databases
InterPro	IPR000742. EG-like_dom. IPR001881. EGF-like_Ca-bd. IPR013032. EGF-like_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_site. IPR018097. EGF_Ca-bd_CS. IPR001007. VWF_C. [Graphical view]
Pfam	PF07645. EGF_CA. 3 hits. PF00093. VWC. 4 hits. [Graphical view]
SMART	SM00181. EGF. 1 hit. SM00179. EGF_CA. 3 hits. SM00214. VWC. 6 hits. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 3 hits. Uncertain. PS00022. EGF_1. 1 hit. PS01186. EGF_2. 2 hits. PS50026. EGF_3. 3 hits. PS01187. EGF_CA. 3 hits. PS01208. VWFC_1. 5 hits. PS50184. VWFC_2. 5 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	220001.
NextBio	90941.
SOURCE	Search...

Entry information

Entry name

VWCE_HUMAN

Accession

Primary (citable) accession number: Q96DN2
Secondary accession number(s): A5PKV0, Q7Z7L6, Q86WK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 24, 2009
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.