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Q96DN2 (VWCE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
von Willebrand factor C and EGF domain-containing protein
Alternative name(s):
HBV X protein up-regulated gene 11 protein
HBxAg up-regulated gene 11 protein
Gene names
Name:VWCE
Synonyms:URG11
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length955 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be a regulatory element in the beta-catenin signaling pathway and a target for chemoprevention of hapatocellular carcinoma. Ref.5

Subcellular location

Secreted Potential. Note: According to Ref.5 is localized in the cytoplasm. Ref.5

Tissue specificity

Expressed in liver. Ref.5

Induction

By HBxAg. Ref.5

Sequence similarities

Contains 4 EGF-like domains.

Contains 6 VWFC domains.

Sequence caution

The sequence AAO47606.1 differs from that shown. Reason: Frameshift at positions 210, 232, 745, 761, 769, 837 and 912.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainEGF-like domain
Repeat
Signal
   LigandCalcium
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96DN2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96DN2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     219-219: V → M
     220-955: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 955934von Willebrand factor C and EGF domain-containing protein
PRO_0000318580

Regions

Domain70 – 9829EGF-like 1
Domain142 – 18039EGF-like 2; calcium-binding Potential
Domain181 – 21939EGF-like 3; calcium-binding Potential
Domain220 – 26243EGF-like 4; calcium-binding Potential
Domain384 – 44158VWFC 1
Domain442 – 50261VWFC 2
Domain503 – 56058VWFC 3
Domain566 – 62661VWFC 4
Domain627 – 68559VWFC 5
Domain686 – 74358VWFC 6
Compositional bias285 – 2884Poly-Leu
Compositional bias835 – 87238Pro-rich

Amino acid modifications

Glycosylation4621N-linked (GlcNAc...) Potential
Glycosylation4721N-linked (GlcNAc...) Potential
Disulfide bond146 ↔ 155 By similarity
Disulfide bond151 ↔ 164 By similarity
Disulfide bond166 ↔ 179 By similarity
Disulfide bond185 ↔ 194 By similarity
Disulfide bond190 ↔ 203 By similarity
Disulfide bond205 ↔ 218 By similarity
Disulfide bond224 ↔ 237 By similarity
Disulfide bond233 ↔ 246 By similarity
Disulfide bond248 ↔ 261 By similarity

Natural variations

Alternative sequence2191V → M in isoform 2.
VSP_031230
Alternative sequence220 – 955736Missing in isoform 2.
VSP_031231
Natural variant8421P → R.
Corresponds to variant rs3750982 [ dbSNP | Ensembl ].
VAR_038782

Experimental info

Sequence conflict1341T → A in BAB71219. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 24, 2009. Version 2.
Checksum: B745618AD37E712B

FASTA95599,915
        10         20         30         40         50         60 
MWAGLLLRAA CVALLLPGAP ARGYTGRKPP GHFAAERRRL GPHVCLSGFG SGCCPGWAPS 

        70         80         90        100        110        120 
MGGGHCTLPL CSFGCGSGIC IAPNVCSCQD GEQGATCPET HGPCGEYGCD LTCNHGGCQE 

       130        140        150        160        170        180 
VARVCPVGFS MTETAVGIRC TDIDECVTSS CEGHCVNTEG GFVCECGPGM QLSADRHSCQ 

       190        200        210        220        230        240 
DTDECLGTPC QQRCKNSIGS YKCSCRTGFH LHGNRHSCVD VNECRRPLER RVCHHSCHNT 

       250        260        270        280        290        300 
VGSFLCTCRP GFRLRADRVS CEAFPKAVLA PSAILQPRQH PSKMLLLLPE AGRPALSPGH 

       310        320        330        340        350        360 
SPPSGAPGPP AGVRTTRLPS PTPRLPTSSP SAPVWLLSTL LATPVPTASL LGNLRPPSLL 

       370        380        390        400        410        420 
QGEVMGTPSS PRGPESPRLA AGPSPCWHLG AMHESRSRWT EPGCSQCWCE DGKVTCEKVR 

       430        440        450        460        470        480 
CEAACSHPIP SRDGGCCPSC TGCFHSGVVR AEGDVFSPPN ENCTVCVCLA GNVSCISPEC 

       490        500        510        520        530        540 
PSGPCQTPPQ TDCCTCVPVR CYFHGRWYAD GAVFSGGGDE CTTCVCQNGE VECSFMPCPE 

       550        560        570        580        590        600 
LACPREEWRL GPGQCCFTCQ EPTPSTGCSL DDNGVEFPIG QIWSPGDPCE LCICQADGSV 

       610        620        630        640        650        660 
SCKRTDCVDS CPHPIRIPGQ CCPDCSAGCT YTGRIFYNNE TFPSVLDPCL SCICLLGSVA 

       670        680        690        700        710        720 
CSPVDCPITC TYPFHPDGEC CPVCRDCNYE GRKVANGQVF TLDDEPCTRC TCQLGEVSCE 

       730        740        750        760        770        780 
KVPCQRACAD PALLPGDCCS SCPDSLSPLE EKQGLSPHGN VAFSKAGRSL HGDTEAPVNC 

       790        800        810        820        830        840 
SSCPGPPTAS PSRPVLHLLQ LLLRTNLMKT QTLPTSPAGA HGPHSLALGL TATFPGEPGA 

       850        860        870        880        890        900 
SPRLSPGPST PPGAPTLPLA SPGAPQPPPV TPERSFSASG AQIVSRWPPL PGTLLTEASA 

       910        920        930        940        950 
LSMMDPSPSK TPITLLGPRV LSPTTSRLST ALAATTHPGP QQPPVGASRG EESTM 

« Hide

Isoform 2 [UniParc].

Checksum: B018610FE43DAE45
Show »

FASTA21922,790

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Teratocarcinoma.
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Lymph.
[4]"Upregulated expression of a unique gene by hepatitis B x antigen promotes hepatocellular growth and tumorigenesis."
Lian Z., Liu J., Li L., Li X., Tufan S.N.L., Clayton M., Wu M.-C., Wang H.-Y., Arbuthnot P., Kew M., Feitelson M.A.
Neoplasia 5:229-244(2003)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-955 (ISOFORM 1).
[5]"Enhanced cell survival of Hep3B cells by the hepatitis B x antigen effector, URG11, is associated with upregulation of beta-catenin."
Lian Z., Liu J., Li L., Li X., Clayton M., Wu M.-C., Wang H.-Y., Arbuthnot P., Kew M., Fan D., Feitelson M.A.
Hepatology 43:415-424(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY HBXAG, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK056571 mRNA. Translation: BAB71219.1.
BC052564 mRNA. No translation available.
BC077719 mRNA. No translation available.
BC142624 mRNA. Translation: AAI42625.1.
CH471076 Genomic DNA. Translation: EAW73932.1.
AY205235 mRNA. Translation: AAO47606.1. Frameshift.
CCDSCCDS8002.1. [Q96DN2-1]
RefSeqNP_689931.2. NM_152718.2. [Q96DN2-1]
UniGeneHs.60640.

3D structure databases

ProteinModelPortalQ96DN2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ96DN2. 1 interaction.
STRING9606.ENSP00000334186.

PTM databases

PhosphoSiteQ96DN2.

Polymorphism databases

DMDM269849477.

Proteomic databases

PaxDbQ96DN2.
PRIDEQ96DN2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000301770; ENSP00000301770; ENSG00000167992. [Q96DN2-2]
ENST00000335613; ENSP00000334186; ENSG00000167992. [Q96DN2-1]
GeneID220001.
KEGGhsa:220001.
UCSCuc001nra.3. human. [Q96DN2-1]

Organism-specific databases

CTD220001.
GeneCardsGC11M061025.
H-InvDBHIX0009684.
HGNCHGNC:26487. VWCE.
HPAHPA040401.
HPA043921.
MIM611115. gene.
neXtProtNX_Q96DN2.
PharmGKBPA143485669.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG240200.
HOGENOMHOG000168460.
HOVERGENHBG067558.
InParanoidQ96DN2.
OMACTCVPVR.
OrthoDBEOG7D2FCX.
PhylomeDBQ96DN2.
TreeFamTF330819.

Gene expression databases

ArrayExpressQ96DN2.
BgeeQ96DN2.
CleanExHS_VWCE.
GenevestigatorQ96DN2.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_N_dom.
IPR001007. VWF_C.
[Graphical view]
PfamPF07645. EGF_CA. 3 hits.
PF00093. VWC. 4 hits.
[Graphical view]
SMARTSM00181. EGF. 1 hit.
SM00179. EGF_CA. 3 hits.
SM00214. VWC. 6 hits.
[Graphical view]
SUPFAMSSF57184. SSF57184. 1 hit.
PROSITEPS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 3 hits.
PS01208. VWFC_1. 5 hits.
PS50184. VWFC_2. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi220001.
NextBio90941.
PROQ96DN2.
SOURCESearch...

Entry information

Entry nameVWCE_HUMAN
AccessionPrimary (citable) accession number: Q96DN2
Secondary accession number(s): A5PKV0, Q7Z7L6, Q86WK8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 24, 2009
Last modified: July 9, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM