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Protein

RNA-binding protein Musashi homolog 2

Gene

MSI2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei217 – 2182Breakpoint for translocation to form MSI2/HOXA9 fusion protein

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. poly(A) RNA binding Source: UniProtKB
  3. poly(U) RNA binding Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein Musashi homolog 2
Short name:
Musashi-2
Gene namesi
Name:MSI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:18585. MSI2.

Subcellular locationi

Cytoplasm
Note: Associated with polysomes.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. polysome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).

Organism-specific databases

PharmGKBiPA38590.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328RNA-binding protein Musashi homolog 2PRO_0000081652Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ96DH6.
PaxDbiQ96DH6.
PRIDEiQ96DH6.

PTM databases

PhosphoSiteiQ96DH6.

Expressioni

Tissue specificityi

Ubiquitous; detected at low levels.1 Publication

Inductioni

Up-regulated in astrocytes after brain injury.By similarity

Gene expression databases

BgeeiQ96DH6.
CleanExiHS_MSI2.
ExpressionAtlasiQ96DH6. baseline and differential.
GenevestigatoriQ96DH6.

Organism-specific databases

HPAiCAB022300.

Interactioni

Protein-protein interaction databases

BioGridi125873. 21 interactions.
IntActiQ96DH6. 3 interactions.
STRINGi9606.ENSP00000284073.

Structurei

3D structure databases

ProteinModelPortaliQ96DH6.
SMRiQ96DH6. Positions 22-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 11191RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini110 – 18778RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi253 – 2608Poly-Ala

Sequence similaritiesi

Belongs to the Musashi family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00730000110584.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiQ96DH6.
KOiK14411.
OMAiVNECTIM.
PhylomeDBiQ96DH6.
TreeFamiTF325419.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96DH6-1) [UniParc]FASTAAdd to Basket

Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEANGSQGTS GSANDSQHDP GKMFIGGLSW QTSPDSLRDY FSKFGEIREC
60 70 80 90 100
MVMRDPTTKR SRGFGFVTFA DPASVDKVLG QPHHELDSKT IDPKVAFPRR
110 120 130 140 150
AQPKMVTRTK KIFVGGLSAN TVVEDVKQYF EQFGKVEDAM LMFDKTTNRH
160 170 180 190 200
RGFGFVTFEN EDVVEKVCEI HFHEINNKMV ECKKAQPKEV MFPPGTRGRA
210 220 230 240 250
RGLPYTMDAF MLGMGMLGYP NFVATYGRGY PGFAPSYGYQ FPGFPAAAYG
260 270 280 290 300
PVAAAAVAAA RGSGSNPARP GGFPGANSPG PVADLYGPAS QDSGVGNYIS
310 320
AASPQPGSGF GHGIAGPLIA TAFTNGYH
Length:328
Mass (Da):35,197
Last modified:December 1, 2001 - v1
Checksum:i6E2FC929491748A2
GO
Isoform 2 (identifier: Q96DH6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MEANGSQGTSGSANDSQHDPG → MADLTSVLTSVMFSPSS
     243-255: GFPAAAYGPVAAA → DYLPVSQDIIFIN
     256-328: Missing.

Note: Initiator Met-1 is removed. Contains a N-acetylalanine at position 2. Contains a phosphoserine at position 14.

Show »
Length:251
Mass (Da):28,421
Checksum:i288CBB2F6E0A2870
GO
Isoform 3 (identifier: Q96DH6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.
     243-255: GFPAAAYGPVAAA → DYLPVSQDIIFIN
     256-328: Missing.

Show »
Length:151
Mass (Da):17,238
Checksum:iF05CDAD5A60DC5B1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 104104Missing in isoform 3. 1 PublicationVSP_011169Add
BLAST
Alternative sequencei1 – 2121MEANG…QHDPG → MADLTSVLTSVMFSPSS in isoform 2. 1 PublicationVSP_011168Add
BLAST
Alternative sequencei243 – 25513GFPAA…PVAAA → DYLPVSQDIIFIN in isoform 2 and isoform 3. 2 PublicationsVSP_011170Add
BLAST
Alternative sequencei256 – 32873Missing in isoform 2 and isoform 3. 2 PublicationsVSP_011171Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC001526 mRNA. Translation: AAH01526.1.
BC017560 mRNA. Translation: AAH17560.1.
AK093888 mRNA. Translation: BAC04244.1.
CCDSiCCDS11596.1. [Q96DH6-1]
CCDS11597.1. [Q96DH6-2]
RefSeqiNP_620412.1. NM_138962.2. [Q96DH6-1]
NP_733839.1. NM_170721.1. [Q96DH6-2]
UniGeneiHs.658922.

Genome annotation databases

EnsembliENST00000284073; ENSP00000284073; ENSG00000153944. [Q96DH6-1]
ENST00000322684; ENSP00000313616; ENSG00000153944. [Q96DH6-2]
ENST00000579180; ENSP00000462264; ENSG00000153944. [Q96DH6-3]
GeneIDi124540.
KEGGihsa:124540.
UCSCiuc002iuz.1. human. [Q96DH6-1]
uc002iva.3. human. [Q96DH6-2]

Polymorphism databases

DMDMi51316513.

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC001526 mRNA. Translation: AAH01526.1.
BC017560 mRNA. Translation: AAH17560.1.
AK093888 mRNA. Translation: BAC04244.1.
CCDSiCCDS11596.1. [Q96DH6-1]
CCDS11597.1. [Q96DH6-2]
RefSeqiNP_620412.1. NM_138962.2. [Q96DH6-1]
NP_733839.1. NM_170721.1. [Q96DH6-2]
UniGeneiHs.658922.

3D structure databases

ProteinModelPortaliQ96DH6.
SMRiQ96DH6. Positions 22-188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125873. 21 interactions.
IntActiQ96DH6. 3 interactions.
STRINGi9606.ENSP00000284073.

PTM databases

PhosphoSiteiQ96DH6.

Polymorphism databases

DMDMi51316513.

Proteomic databases

MaxQBiQ96DH6.
PaxDbiQ96DH6.
PRIDEiQ96DH6.

Protocols and materials databases

DNASUi124540.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000284073; ENSP00000284073; ENSG00000153944. [Q96DH6-1]
ENST00000322684; ENSP00000313616; ENSG00000153944. [Q96DH6-2]
ENST00000579180; ENSP00000462264; ENSG00000153944. [Q96DH6-3]
GeneIDi124540.
KEGGihsa:124540.
UCSCiuc002iuz.1. human. [Q96DH6-1]
uc002iva.3. human. [Q96DH6-2]

Organism-specific databases

CTDi124540.
GeneCardsiGC17P055334.
HGNCiHGNC:18585. MSI2.
HPAiCAB022300.
MIMi607897. gene.
neXtProtiNX_Q96DH6.
PharmGKBiPA38590.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00730000110584.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiQ96DH6.
KOiK14411.
OMAiVNECTIM.
PhylomeDBiQ96DH6.
TreeFamiTF325419.

Miscellaneous databases

ChiTaRSiMSI2. human.
GenomeRNAii124540.
NextBioi81316.
PROiQ96DH6.
SOURCEiSearch...

Gene expression databases

BgeeiQ96DH6.
CleanExiHS_MSI2.
ExpressionAtlasiQ96DH6. baseline and differential.
GenevestigatoriQ96DH6.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Trachea.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Placenta and Skin.
  3. "A novel gene, MSI2, encoding a putative RNA-binding protein is recurrently rearranged at disease progression of chronic myeloid leukemia and forms a fusion gene with HOXA9 as a result of the cryptic t(7;17)(p15;q23)."
    Barbouti A., Hoeglund M., Johansson B., Lassen C., Nilsson P.-G., Hagemeijer A., Mitelman F., Fioretos T.
    Cancer Res. 63:1202-1206(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHROMOSOMAL TRANSLOCATION WITH HOXA9, TISSUE SPECIFICITY.
  4. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2 (ISOFORM 2), PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMSI2H_HUMAN
AccessioniPrimary (citable) accession number: Q96DH6
Secondary accession number(s): Q7Z6M7, Q8N9T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 1, 2001
Last modified: February 4, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.