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Q96DH6 (MSI2H_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-binding protein Musashi homolog 2

Short name=Musashi-2
Gene names
Name:MSI2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity.

Subcellular location

Cytoplasm. Note: Associated with polysomes By similarity.

Tissue specificity

Ubiquitous; detected at low levels. Ref.3

Induction

Up-regulated in astrocytes after brain injury By similarity.

Post-translational modification

Phosphorylated By similarity.

Involvement in disease

Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).

Sequence similarities

Belongs to the Musashi family.

Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
Chromosomal rearrangement
   DomainRepeat
   LigandRNA-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processstem cell development

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

polysome

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionnucleotide binding

Inferred from electronic annotation. Source: InterPro

poly(A) RNA binding

Inferred from direct assay PubMed 22658674PubMed 22681889. Source: UniProtKB

poly(U) RNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96DH6-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96DH6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MEANGSQGTSGSANDSQHDPG → MADLTSVLTSVMFSPSS
     243-255: GFPAAAYGPVAAA → DYLPVSQDIIFIN
     256-328: Missing.
Note: Initiator Met-1 is removed. Contains a N-acetylalanine at position 2. Contains a phosphoserine at position 14.
Isoform 3 (identifier: Q96DH6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.
     243-255: GFPAAAYGPVAAA → DYLPVSQDIIFIN
     256-328: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328RNA-binding protein Musashi homolog 2
PRO_0000081652

Regions

Domain21 – 11191RRM 1
Domain110 – 18778RRM 2
Compositional bias253 – 2608Poly-Ala

Sites

Site217 – 2182Breakpoint for translocation to form MSI2/HOXA9 fusion protein

Amino acid modifications

Modified residue11N-acetylmethionine Ref.5

Natural variations

Alternative sequence1 – 104104Missing in isoform 3.
VSP_011169
Alternative sequence1 – 2121MEANG…QHDPG → MADLTSVLTSVMFSPSS in isoform 2.
VSP_011168
Alternative sequence243 – 25513GFPAA…PVAAA → DYLPVSQDIIFIN in isoform 2 and isoform 3.
VSP_011170
Alternative sequence256 – 32873Missing in isoform 2 and isoform 3.
VSP_011171

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (A) [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 6E2FC929491748A2

FASTA32835,197
        10         20         30         40         50         60 
MEANGSQGTS GSANDSQHDP GKMFIGGLSW QTSPDSLRDY FSKFGEIREC MVMRDPTTKR 

        70         80         90        100        110        120 
SRGFGFVTFA DPASVDKVLG QPHHELDSKT IDPKVAFPRR AQPKMVTRTK KIFVGGLSAN 

       130        140        150        160        170        180 
TVVEDVKQYF EQFGKVEDAM LMFDKTTNRH RGFGFVTFEN EDVVEKVCEI HFHEINNKMV 

       190        200        210        220        230        240 
ECKKAQPKEV MFPPGTRGRA RGLPYTMDAF MLGMGMLGYP NFVATYGRGY PGFAPSYGYQ 

       250        260        270        280        290        300 
FPGFPAAAYG PVAAAAVAAA RGSGSNPARP GGFPGANSPG PVADLYGPAS QDSGVGNYIS 

       310        320 
AASPQPGSGF GHGIAGPLIA TAFTNGYH 

« Hide

Isoform 2 [UniParc].

Checksum: 288CBB2F6E0A2870
Show »

FASTA25128,421
Isoform 3 [UniParc].

Checksum: F05CDAD5A60DC5B1
Show »

FASTA15117,238

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Trachea.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Placenta and Skin.
[3]"A novel gene, MSI2, encoding a putative RNA-binding protein is recurrently rearranged at disease progression of chronic myeloid leukemia and forms a fusion gene with HOXA9 as a result of the cryptic t(7;17)(p15;q23)."
Barbouti A., Hoeglund M., Johansson B., Lassen C., Nilsson P.-G., Hagemeijer A., Mitelman F., Fioretos T.
Cancer Res. 63:1202-1206(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: CHROMOSOMAL TRANSLOCATION WITH HOXA9, TISSUE SPECIFICITY.
[4]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2 (ISOFORM 2), PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC001526 mRNA. Translation: AAH01526.1.
BC017560 mRNA. Translation: AAH17560.1.
AK093888 mRNA. Translation: BAC04244.1.
CCDSCCDS11596.1. [Q96DH6-1]
CCDS11597.1. [Q96DH6-2]
RefSeqNP_620412.1. NM_138962.2. [Q96DH6-1]
NP_733839.1. NM_170721.1. [Q96DH6-2]
UniGeneHs.658922.

3D structure databases

ProteinModelPortalQ96DH6.
SMRQ96DH6. Positions 22-188.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125873. 13 interactions.
IntActQ96DH6. 3 interactions.
STRING9606.ENSP00000284073.

PTM databases

PhosphoSiteQ96DH6.

Polymorphism databases

DMDM51316513.

Proteomic databases

MaxQBQ96DH6.
PaxDbQ96DH6.
PRIDEQ96DH6.

Protocols and materials databases

DNASU124540.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000284073; ENSP00000284073; ENSG00000153944. [Q96DH6-1]
ENST00000322684; ENSP00000313616; ENSG00000153944. [Q96DH6-2]
ENST00000579180; ENSP00000462264; ENSG00000153944. [Q96DH6-3]
GeneID124540.
KEGGhsa:124540.
UCSCuc002iuz.1. human. [Q96DH6-1]
uc002iva.3. human. [Q96DH6-2]

Organism-specific databases

CTD124540.
GeneCardsGC17P055334.
HGNCHGNC:18585. MSI2.
HPACAB022300.
MIM607897. gene.
neXtProtNX_Q96DH6.
PharmGKBPA38590.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0724.
HOGENOMHOG000234441.
HOVERGENHBG002295.
InParanoidQ96DH6.
KOK14411.
OMAISDCTIM.
PhylomeDBQ96DH6.
TreeFamTF325419.

Gene expression databases

ArrayExpressQ96DH6.
BgeeQ96DH6.
CleanExHS_MSI2.
GenevestigatorQ96DH6.

Family and domain databases

Gene3D3.30.70.330. 2 hits.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTSM00360. RRM. 2 hits.
[Graphical view]
PROSITEPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMSI2. human.
GenomeRNAi124540.
NextBio81316.
PROQ96DH6.
SOURCESearch...

Entry information

Entry nameMSI2H_HUMAN
AccessionPrimary (citable) accession number: Q96DH6
Secondary accession number(s): Q7Z6M7, Q8N9T4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM