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Protein

Netrin-G2

Gene

NTNG2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Enzyme and pathway databases

BioCyciZFISH:G66-31465-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Netrin-G2
Alternative name(s):
Laminet-2
Gene namesi
Name:NTNG2
Synonyms:KIAA1857, LMNT2
ORF Names:UNQ9381/PRO34206
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:14288. NTNG2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi84628.
OpenTargetsiENSG00000196358.
PharmGKBiPA134962540.

Polymorphism and mutation databases

BioMutaiNTNG2.
DMDMi317373402.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000001709518 – 507Netrin-G2Add BLAST490
PropeptideiPRO_0000017096508 – 530Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 391 Publication
Disulfide bondi61 ↔ 811 Publication
Disulfide bondi69 ↔ 771 Publication
Glycosylationi122N-linked (GlcNAc...)1 Publication1
Glycosylationi128N-linked (GlcNAc...)1 Publication1
Disulfide bondi171 ↔ 1951 Publication
Disulfide bondi287 ↔ 2961 Publication
Disulfide bondi289 ↔ 3051 Publication
Disulfide bondi307 ↔ 3161 Publication
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi319 ↔ 3441 Publication
Disulfide bondi353 ↔ 362Sequence analysis
Disulfide bondi355 ↔ 373Sequence analysis
Disulfide bondi376 ↔ 385Sequence analysis
Disulfide bondi388 ↔ 406Sequence analysis
Glycosylationi395N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi409 ↔ 421Sequence analysis
Disulfide bondi411 ↔ 427Sequence analysis
Glycosylationi422N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi429 ↔ 438Sequence analysis
Disulfide bondi441 ↔ 451Sequence analysis
Disulfide bondi456 ↔ 469By similarity
Disulfide bondi463 ↔ 475By similarity
Disulfide bondi477 ↔ 486By similarity
Lipidationi507GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ96CW9.
PeptideAtlasiQ96CW9.
PRIDEiQ96CW9.

PTM databases

iPTMnetiQ96CW9.
PhosphoSitePlusiQ96CW9.

Expressioni

Gene expression databases

BgeeiENSG00000196358.
CleanExiHS_NTNG2.
GenevisibleiQ96CW9. HS.

Organism-specific databases

HPAiHPA071290.

Interactioni

Subunit structurei

Interacts with LRRC4.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
LRRC4Q9HBW14EBI-750795,EBI-7444327
LRRC4CQ9HCJ22EBI-750795,EBI-3925442

Protein-protein interaction databases

BioGridi124157. 2 interactors.
IntActiQ96CW9. 4 interactors.
MINTiMINT-7969778.
STRINGi9606.ENSP00000376921.

Structurei

Secondary structure

1530
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 28Combined sources7
Beta strandi31 – 36Combined sources6
Helixi46 – 49Combined sources4
Beta strandi51 – 56Combined sources6
Beta strandi66 – 68Combined sources3
Beta strandi71 – 73Combined sources3
Turni74 – 76Combined sources3
Beta strandi79 – 81Combined sources3
Beta strandi83 – 85Combined sources3
Turni86 – 88Combined sources3
Helixi92 – 95Combined sources4
Beta strandi107 – 109Combined sources3
Turni113 – 116Combined sources4
Beta strandi121 – 133Combined sources13
Beta strandi137 – 143Combined sources7
Beta strandi147 – 156Combined sources10
Beta strandi162 – 170Combined sources9
Helixi171 – 175Combined sources5
Helixi182 – 184Combined sources3
Helixi187 – 189Combined sources3
Turni198 – 204Combined sources7
Beta strandi205 – 207Combined sources3
Beta strandi210 – 213Combined sources4
Helixi216 – 223Combined sources8
Turni224 – 227Combined sources4
Helixi230 – 239Combined sources10
Helixi243 – 246Combined sources4
Beta strandi248 – 258Combined sources11
Helixi271 – 273Combined sources3
Beta strandi277 – 282Combined sources6
Beta strandi284 – 287Combined sources4
Beta strandi296 – 299Combined sources4
Beta strandi302 – 305Combined sources4
Beta strandi311 – 313Combined sources3
Beta strandi324 – 326Combined sources3
Turni336 – 338Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TBDX-ray1.80A18-349[»]
3ZYGX-ray2.20A/B1-345[»]
3ZYIX-ray2.60B1-345[»]
ProteinModelPortaliQ96CW9.
SMRiQ96CW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 286Laminin N-terminalPROSITE-ProRule annotationAdd BLAST252
Domaini287 – 346Laminin EGF-like 1PROSITE-ProRule annotationAdd BLAST60
Domaini353 – 408Laminin EGF-like 2PROSITE-ProRule annotationAdd BLAST56
Domaini409 – 453Laminin EGF-like 3PROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 88NGL discriminant loop IAdd BLAST20
Regioni201 – 203NGL discriminant loop II3
Regioni264 – 267NGL discriminant loop III4

Domaini

The laminin N-terminal domain mediates 1:1 binding to NGL ligand with sub-micromolar affinity. Three NGL-binding loops mediate discrimination for LRRC4C/NGL1 among other NGLs by binding specifically to its LRR repeats. This specificity drives the sorting of a mixed population of molecules into discrete cell surface subdomains.

Sequence similaritiesi

Contains 3 laminin EGF-like domains.PROSITE-ProRule annotationCurated
Contains 1 laminin N-terminal domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3512. Eukaryota.
ENOG410XS7U. LUCA.
GeneTreeiENSGT00840000129717.
HOGENOMiHOG000231614.
HOVERGENiHBG052676.
InParanoidiQ96CW9.
KOiK16359.
OMAiNCECYGH.
OrthoDBiEOG091G05AV.
PhylomeDBiQ96CW9.
TreeFamiTF333945.

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR002049. Laminin_EGF.
IPR008211. Laminin_N.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01248. EGF_LAM_1. 2 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96CW9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLHLLALFLH CLPLASGDYD ICKSWVTTDE GPTWEFYACQ PKVMRLKDYV
60 70 80 90 100
KVKVEPSGIT CGDPPERFCS HENPYLCSNE CDASNPDLAH PPRLMFDKEE
110 120 130 140 150
EGLATYWQSI TWSRYPSPLE ANITLSWNKT VELTDDVVMT FEYGRPTVMV
160 170 180 190 200
LEKSLDNGRT WQPYQFYAED CMEAFGMSAR RARDMSSSSA HRVLCTEEYS
210 220 230 240 250
RWAGSKKEKH VRFEVRDRFA IFAGPDLRNM DNLYTRLESA KGLKEFFTLT
260 270 280 290 300
DLRMRLLRPA LGGTYVQREN LYKYFYAISN IEVIGRCKCN LHANLCSMRE
310 320 330 340 350
GSLQCECEHN TTGPDCGKCK KNFRTRSWRA GSYLPLPHGS PNACATAGSF
360 370 380 390 400
GNCECYGHSN RCSYIDFLNV VTCVSCKHNT RGQHCQHCRL GYYRNGSAEL
410 420 430 440 450
DDENVCIECN CNQIGSVHDR CNETGFCECR EGAAGPKCDD CLPTHYWRQG
460 470 480 490 500
CYPNVCDDDQ LLCQNGGTCL QNQRCACPRG YTGVRCEQPR CDPADDDGGL
510 520 530
DCDRAPGAAP RPATLLGCLL LLGLAARLGR
Length:530
Mass (Da):59,799
Last modified:January 11, 2011 - v2
Checksum:iB93A8A7E0E5A8CA3
GO
Isoform 2 (identifier: Q96CW9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     352-422: NCECYGHSNR...QIGSVHDRCN → TLQTPPPGRS...DPGGPWLGSQ
     423-530: Missing.

Note: No experimental confirmation available.
Show »
Length:422
Mass (Da):47,491
Checksum:i9A4B38913B097AF9
GO

Sequence cautioni

The sequence BAB47486 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_047847346T → A.3 PublicationsCorresponds to variant rs4962173dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013147352 – 422NCECY…HDRCN → TLQTPPPGRSPSALRGSRRG LANVKEPAGSRPQISEMLLG CTVTLHQGSVGPHIPPKLSL PDPGGPWLGSQ in isoform 2. 1 PublicationAdd BLAST71
Alternative sequenceiVSP_013148423 – 530Missing in isoform 2. 1 PublicationAdd BLAST108

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058760 mRNA. Translation: BAB47486.2. Different initiation.
AY358165 mRNA. Translation: AAQ88532.1.
AL353631, AL159997 Genomic DNA. Translation: CAI16125.1.
AL159997, AL353631 Genomic DNA. Translation: CAI40856.1.
BC013770 mRNA. Translation: AAH13770.1.
CCDSiCCDS6946.1. [Q96CW9-1]
RefSeqiNP_115925.2. NM_032536.2. [Q96CW9-1]
XP_011517409.1. XM_011519107.2. [Q96CW9-1]
UniGeneiHs.163642.

Genome annotation databases

EnsembliENST00000372179; ENSP00000361252; ENSG00000196358. [Q96CW9-2]
ENST00000393229; ENSP00000376921; ENSG00000196358. [Q96CW9-1]
GeneIDi84628.
KEGGihsa:84628.
UCSCiuc004cbh.3. human. [Q96CW9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058760 mRNA. Translation: BAB47486.2. Different initiation.
AY358165 mRNA. Translation: AAQ88532.1.
AL353631, AL159997 Genomic DNA. Translation: CAI16125.1.
AL159997, AL353631 Genomic DNA. Translation: CAI40856.1.
BC013770 mRNA. Translation: AAH13770.1.
CCDSiCCDS6946.1. [Q96CW9-1]
RefSeqiNP_115925.2. NM_032536.2. [Q96CW9-1]
XP_011517409.1. XM_011519107.2. [Q96CW9-1]
UniGeneiHs.163642.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TBDX-ray1.80A18-349[»]
3ZYGX-ray2.20A/B1-345[»]
3ZYIX-ray2.60B1-345[»]
ProteinModelPortaliQ96CW9.
SMRiQ96CW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124157. 2 interactors.
IntActiQ96CW9. 4 interactors.
MINTiMINT-7969778.
STRINGi9606.ENSP00000376921.

PTM databases

iPTMnetiQ96CW9.
PhosphoSitePlusiQ96CW9.

Polymorphism and mutation databases

BioMutaiNTNG2.
DMDMi317373402.

Proteomic databases

PaxDbiQ96CW9.
PeptideAtlasiQ96CW9.
PRIDEiQ96CW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372179; ENSP00000361252; ENSG00000196358. [Q96CW9-2]
ENST00000393229; ENSP00000376921; ENSG00000196358. [Q96CW9-1]
GeneIDi84628.
KEGGihsa:84628.
UCSCiuc004cbh.3. human. [Q96CW9-1]

Organism-specific databases

CTDi84628.
DisGeNETi84628.
GeneCardsiNTNG2.
H-InvDBHIX0008489.
HGNCiHGNC:14288. NTNG2.
HPAiHPA071290.
neXtProtiNX_Q96CW9.
OpenTargetsiENSG00000196358.
PharmGKBiPA134962540.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3512. Eukaryota.
ENOG410XS7U. LUCA.
GeneTreeiENSGT00840000129717.
HOGENOMiHOG000231614.
HOVERGENiHBG052676.
InParanoidiQ96CW9.
KOiK16359.
OMAiNCECYGH.
OrthoDBiEOG091G05AV.
PhylomeDBiQ96CW9.
TreeFamiTF333945.

Enzyme and pathway databases

BioCyciZFISH:G66-31465-MONOMER.

Miscellaneous databases

GeneWikiiNTNG2.
GenomeRNAii84628.
PROiQ96CW9.

Gene expression databases

BgeeiENSG00000196358.
CleanExiHS_NTNG2.
GenevisibleiQ96CW9. HS.

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR002049. Laminin_EGF.
IPR008211. Laminin_N.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01248. EGF_LAM_1. 2 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTNG2_HUMAN
AccessioniPrimary (citable) accession number: Q96CW9
Secondary accession number(s): Q5JUJ2, Q6UXY0, Q96JH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: January 11, 2011
Last modified: November 2, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.