Q96CW9 (NTNG2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Netrin-G2

Alternative name(s):
Laminet-2

Gene names

Name:	NTNG2
Synonyms:	KIAA1857, LMNT2
ORF Names:	UNQ9381/PRO34206

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites. Ref.6
Subunit structure	Interacts with LRRC4 By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side Ref.6.
Domain	The laminin N-terminal domain mediates 1:1 binding to NGL ligand with sub-micromolar affinity. Three NGL-binding loops mediate discrimination for LRRC4C/NGL1 among other NGLs by binding specifically to its LRR repeats. This specificity drives the sorting of a mixed population of molecules into discrete cell surface subdomains.
Post-translational modification	N-glycosylated By similarity. Ref.7 UniProtKB Q8R4F1
Sequence similarities	Contains 3 laminin EGF-like domains. Contains 1 laminin N-terminal domain.
Sequence caution	The sequence BAB47486.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Laminin EGF-like domain Repeat Signal
Molecular function	Developmental protein
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axonogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	anchored to plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96CW9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96CW9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     352-422: NCECYGHSNR...QIGSVHDRCN → TLQTPPPGRS...DPGGPWLGSQ
     423-530: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Potential

Chain

18 – 507

490

Netrin-G2

PRO_0000017095

Propeptide

508 – 530

23

Removed in mature form Potential

PRO_0000017096

Regions

Domain

35 – 286

252

Laminin N-terminal

Domain

287 – 346

60

Laminin EGF-like 1

Domain

353 – 408

56

Laminin EGF-like 2

Domain

409 – 453

45

Laminin EGF-like 3

Region

69 – 88

20

NGL discriminant loop I

Region

201 – 203

3

NGL discriminant loop II

Region

264 – 267

4

NGL discriminant loop III

Amino acid modifications

Lipidation

507

1

GPI-anchor amidated glycine Potential

Glycosylation

122

1

N-linked (GlcNAc...) Ref.7

Glycosylation

128

1

N-linked (GlcNAc...) Ref.7

Glycosylation

310

1

N-linked (GlcNAc...) Potential

Glycosylation

395

1

N-linked (GlcNAc...) Potential

Glycosylation

422

1

N-linked (GlcNAc...) Potential

Disulfide bond

22 ↔ 39

Ref.7

Disulfide bond

61 ↔ 81

Ref.7

Disulfide bond

69 ↔ 77

Ref.7

Disulfide bond

171 ↔ 195

Ref.7

Disulfide bond

287 ↔ 296

Ref.7

Disulfide bond

289 ↔ 305

Ref.7

Disulfide bond

307 ↔ 316

Ref.7

Disulfide bond

319 ↔ 344

Ref.7

Disulfide bond

353 ↔ 362

Potential

Disulfide bond

355 ↔ 373

Potential

Disulfide bond

376 ↔ 385

Potential

Disulfide bond

388 ↔ 406

Potential

Disulfide bond

409 ↔ 421

Potential

Disulfide bond

411 ↔ 427

Potential

Disulfide bond

429 ↔ 438

Potential

Disulfide bond

441 ↔ 451

Potential

Disulfide bond

456 ↔ 469

By similarity

Disulfide bond

463 ↔ 475

By similarity

Disulfide bond

477 ↔ 486

By similarity

Natural variations

Alternative sequence

352 – 422

71

NCECY…HDRCN → TLQTPPPGRSPSALRGSRRG LANVKEPAGSRPQISEMLLG CTVTLHQGSVGPHIPPKLSL PDPGGPWLGSQ in isoform 2.

VSP_013147

Alternative sequence

423 – 530

108

Missing in isoform 2.

VSP_013148

Natural variant

346

1

T → A. Ref.1 Ref.3 Ref.5
Corresponds to variant rs4962173 [ dbSNP | Ensembl ].

VAR_047847

Secondary structure

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530

Helix Strand Turn

Details...

Beta strand

22 – 28

7

Beta strand

31 – 36

6

Helix

46 – 49

4

Beta strand

51 – 56

6

Beta strand

66 – 68

3

Beta strand

71 – 73

3

Turn

74 – 76

3

Beta strand

79 – 81

3

Beta strand

83 – 85

3

Turn

86 – 88

3

Helix

92 – 95

4

Beta strand

107 – 109

3

Turn

113 – 116

4

Beta strand

121 – 133

13

Beta strand

137 – 143

7

Beta strand

147 – 156

10

Beta strand

162 – 170

9

Helix

171 – 175

5

Helix

182 – 184

3

Helix

187 – 189

3

Turn

198 – 204

7

Beta strand

205 – 207

3

Beta strand

210 – 213

4

Helix

216 – 223

8

Turn

224 – 227

4

Helix

230 – 239

10

Helix

243 – 246

4

Beta strand

248 – 258

11

Helix

271 – 273

3

Beta strand

277 – 282

6

Beta strand

284 – 287

4

Beta strand

296 – 299

4

Beta strand

302 – 305

4

Beta strand

311 – 313

3

Beta strand

324 – 326

3

Turn

336 – 338

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 11, 2011. Version 2. Checksum: B93A8A7E0E5A8CA3	FASTA	530	59,799
	10 20 30 40 50 60 MLHLLALFLH CLPLASGDYD ICKSWVTTDE GPTWEFYACQ PKVMRLKDYV KVKVEPSGIT 70 80 90 100 110 120 CGDPPERFCS HENPYLCSNE CDASNPDLAH PPRLMFDKEE EGLATYWQSI TWSRYPSPLE 130 140 150 160 170 180 ANITLSWNKT VELTDDVVMT FEYGRPTVMV LEKSLDNGRT WQPYQFYAED CMEAFGMSAR 190 200 210 220 230 240 RARDMSSSSA HRVLCTEEYS RWAGSKKEKH VRFEVRDRFA IFAGPDLRNM DNLYTRLESA 250 260 270 280 290 300 KGLKEFFTLT DLRMRLLRPA LGGTYVQREN LYKYFYAISN IEVIGRCKCN LHANLCSMRE 310 320 330 340 350 360 GSLQCECEHN TTGPDCGKCK KNFRTRSWRA GSYLPLPHGS PNACATAGSF GNCECYGHSN 370 380 390 400 410 420 RCSYIDFLNV VTCVSCKHNT RGQHCQHCRL GYYRNGSAEL DDENVCIECN CNQIGSVHDR 430 440 450 460 470 480 CNETGFCECR EGAAGPKCDD CLPTHYWRQG CYPNVCDDDQ LLCQNGGTCL QNQRCACPRG 490 500 510 520 530 YTGVRCEQPR CDPADDDGGL DCDRAPGAAP RPATLLGCLL LLGLAARLGR
	Isoform 2 [UniParc]. Checksum: 9A4B38913B097AF9	FASTA	422	47,491
	10 20 30 40 50 60 MLHLLALFLH CLPLASGDYD ICKSWVTTDE GPTWEFYACQ PKVMRLKDYV KVKVEPSGIT 70 80 90 100 110 120 CGDPPERFCS HENPYLCSNE CDASNPDLAH PPRLMFDKEE EGLATYWQSI TWSRYPSPLE 130 140 150 160 170 180 ANITLSWNKT VELTDDVVMT FEYGRPTVMV LEKSLDNGRT WQPYQFYAED CMEAFGMSAR 190 200 210 220 230 240 RARDMSSSSA HRVLCTEEYS RWAGSKKEKH VRFEVRDRFA IFAGPDLRNM DNLYTRLESA 250 260 270 280 290 300 KGLKEFFTLT DLRMRLLRPA LGGTYVQREN LYKYFYAISN IEVIGRCKCN LHANLCSMRE 310 320 330 340 350 360 GSLQCECEHN TTGPDCGKCK KNFRTRSWRA GSYLPLPHGS PNACATAGSF GTLQTPPPGR 370 380 390 400 410 420 SPSALRGSRR GLANVKEPAG SRPQISEMLL GCTVTLHQGS VGPHIPPKLS LPDPGGPWLG SQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O. DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-346. Tissue: Brain.
[2]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ALA-346.
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-346. Tissue: Adrenal gland.
[6]	"Structural basis for cell surface patterning through NetrinG-NGL interactions." Seiradake E., Coles C.H., Perestenko P.V., Harlos K., McIlhinney R.A., Aricescu A.R., Jones E.Y. EMBO J. 30:4479-4488(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-345 ALONE AND IN COMPLEX WITH LRRC4, FUNCTION, SUBCELLULAR LOCATION.
[7]	"Crystal structure of the ligand binding domain of netrin G2." Brasch J., Harrison O.J., Ahlsen G., Liu Q., Shapiro L. J. Mol. Biol. 414:723-734(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 18-349, DISULFIDE BONDS, GLYCOSYLATION AT ASN-122 AND ASN-128.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB058760 mRNA. Translation: BAB47486.2. Different initiation.
AY358165 mRNA. Translation: AAQ88532.1.
AL353631, AL159997 Genomic DNA. Translation: CAI16125.1.
AL159997, AL353631 Genomic DNA. Translation: CAI40856.1.
BC013770 mRNA. Translation: AAH13770.1.

IPI

IPI00432766.
IPI00843936.

RefSeq

NP_115925.2. NM_032536.2.

UniGene

Hs.163642.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3TBD	X-ray	1.80	A	18-349	[»]
3ZYG	X-ray	2.20	A/B	1-345	[»]
3ZYI	X-ray	2.60	B	1-345	[»]

ProteinModelPortal

Q96CW9.

SMR

Q96CW9. Positions 18-499.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96CW9. 1 interaction.

STRING

9606.ENSP00000376921.

PTM databases

PhosphoSite

Q96CW9.

Polymorphism databases

DMDM

34922606.

Proteomic databases

PaxDb

Q96CW9.

PRIDE

Q96CW9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000372179; ENSP00000361252; ENSG00000196358.
ENST00000393229; ENSP00000376921; ENSG00000196358.

GeneID

84628.

KEGG

hsa:84628.

UCSC

uc004cbh.2. human.

Organism-specific databases

CTD

84628.

GeneCards

GC09P135037.

H-InvDB

HIX0008489.

HGNC

HGNC:14288. NTNG2.

neXtProt

NX_Q96CW9.

PharmGKB

PA134962540.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG286598.

HOGENOM

HOG000231614.

HOVERGEN

HBG052676.

KO

K16359.

OrthoDB

EOG47D9FR.

Gene expression databases

ArrayExpress

Q96CW9.

Bgee

Q96CW9.

CleanEx

HS_NTNG2.

Genevestigator

Q96CW9.

GermOnline

ENSG00000196358. Homo sapiens.

Family and domain databases

InterPro

IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR013111. EGF_extracell.
IPR002049. EGF_laminin.
IPR008211. Laminin_N.
[Graphical view]

Pfam

PF07974. EGF_2. 1 hit.
PF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
[Graphical view]

SMART

SM00181. EGF. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]

PROSITE

PS00022. EGF_1. 3 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01248. EGF_LAM_1. 2 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

84628.

NextBio

74534.

Entry information

Entry name

NTNG2_HUMAN

Accession

Primary (citable) accession number: Q96CW9
Secondary accession number(s): Q5JUJ2, Q6UXY0, Q96JH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.