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Q96CW9

- NTNG2_HUMAN

UniProt

Q96CW9 - NTNG2_HUMAN

Protein

Netrin-G2

Gene

NTNG2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. axonogenesis Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Netrin-G2
    Alternative name(s):
    Laminet-2
    Gene namesi
    Name:NTNG2
    Synonyms:KIAA1857, LMNT2
    ORF Names:UNQ9381/PRO34206
    OrganismiHomo sapiens (Human)Imported
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:14288. NTNG2.

    Subcellular locationi

    Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication; Extracellular side 1 Publication

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134962540.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 507490Netrin-G2PRO_0000017095Add
    BLAST
    Propeptidei508 – 53023Removed in mature formSequence AnalysisPRO_0000017096Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi22 ↔ 391 Publication
    Disulfide bondi61 ↔ 811 Publication
    Disulfide bondi69 ↔ 771 Publication
    Glycosylationi122 – 1221N-linked (GlcNAc...)1 Publication
    Glycosylationi128 – 1281N-linked (GlcNAc...)1 Publication
    Disulfide bondi171 ↔ 1951 Publication
    Disulfide bondi287 ↔ 2961 Publication
    Disulfide bondi289 ↔ 3051 Publication
    Disulfide bondi307 ↔ 3161 Publication
    Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi319 ↔ 3441 Publication
    Disulfide bondi353 ↔ 362Sequence Analysis
    Disulfide bondi355 ↔ 373Sequence Analysis
    Disulfide bondi376 ↔ 385Sequence Analysis
    Disulfide bondi388 ↔ 406Sequence Analysis
    Glycosylationi395 – 3951N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi409 ↔ 421Sequence Analysis
    Disulfide bondi411 ↔ 427Sequence Analysis
    Glycosylationi422 – 4221N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi429 ↔ 438Sequence Analysis
    Disulfide bondi441 ↔ 451Sequence Analysis
    Disulfide bondi456 ↔ 469By similarity
    Disulfide bondi463 ↔ 475By similarity
    Disulfide bondi477 ↔ 486By similarity
    Lipidationi507 – 5071GPI-anchor amidated glycineSequence Analysis

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ96CW9.
    PRIDEiQ96CW9.

    PTM databases

    PhosphoSiteiQ96CW9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ96CW9.
    BgeeiQ96CW9.
    CleanExiHS_NTNG2.
    GenevestigatoriQ96CW9.

    Interactioni

    Subunit structurei

    Interacts with LRRC4.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LRRC4Q9HBW14EBI-750795,EBI-7444327
    LRRC4CQ9HCJ22EBI-750795,EBI-3925442

    Protein-protein interaction databases

    BioGridi124157. 2 interactions.
    IntActiQ96CW9. 4 interactions.
    MINTiMINT-7969778.
    STRINGi9606.ENSP00000376921.

    Structurei

    Secondary structure

    1
    530
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi22 – 287
    Beta strandi31 – 366
    Helixi46 – 494
    Beta strandi51 – 566
    Beta strandi66 – 683
    Beta strandi71 – 733
    Turni74 – 763
    Beta strandi79 – 813
    Beta strandi83 – 853
    Turni86 – 883
    Helixi92 – 954
    Beta strandi107 – 1093
    Turni113 – 1164
    Beta strandi121 – 13313
    Beta strandi137 – 1437
    Beta strandi147 – 15610
    Beta strandi162 – 1709
    Helixi171 – 1755
    Helixi182 – 1843
    Helixi187 – 1893
    Turni198 – 2047
    Beta strandi205 – 2073
    Beta strandi210 – 2134
    Helixi216 – 2238
    Turni224 – 2274
    Helixi230 – 23910
    Helixi243 – 2464
    Beta strandi248 – 25811
    Helixi271 – 2733
    Beta strandi277 – 2826
    Beta strandi284 – 2874
    Beta strandi296 – 2994
    Beta strandi302 – 3054
    Beta strandi311 – 3133
    Beta strandi324 – 3263
    Turni336 – 3383

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TBDX-ray1.80A18-349[»]
    3ZYGX-ray2.20A/B1-345[»]
    3ZYIX-ray2.60B1-345[»]
    ProteinModelPortaliQ96CW9.
    SMRiQ96CW9. Positions 18-527.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 286252Laminin N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini287 – 34660Laminin EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini353 – 40856Laminin EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini409 – 45345Laminin EGF-like 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni69 – 8820NGL discriminant loop IAdd
    BLAST
    Regioni201 – 2033NGL discriminant loop II
    Regioni264 – 2674NGL discriminant loop III

    Domaini

    The laminin N-terminal domain mediates 1:1 binding to NGL ligand with sub-micromolar affinity. Three NGL-binding loops mediate discrimination for LRRC4C/NGL1 among other NGLs by binding specifically to its LRR repeats. This specificity drives the sorting of a mixed population of molecules into discrete cell surface subdomains.

    Sequence similaritiesi

    Contains 3 laminin EGF-like domains.CuratedPROSITE-ProRule annotation
    Contains 1 laminin N-terminal domain.CuratedPROSITE-ProRule annotation

    Keywords - Domaini

    Laminin EGF-like domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG286598.
    HOGENOMiHOG000231614.
    HOVERGENiHBG052676.
    KOiK16359.
    OrthoDBiEOG7HQN7W.
    PhylomeDBiQ96CW9.
    TreeFamiTF333945.

    Family and domain databases

    InterProiIPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR002049. EGF_laminin.
    IPR008211. Laminin_N.
    [Graphical view]
    PfamiPF00053. Laminin_EGF. 3 hits.
    PF00055. Laminin_N. 1 hit.
    [Graphical view]
    SMARTiSM00181. EGF. 1 hit.
    SM00180. EGF_Lam. 3 hits.
    SM00136. LamNT. 1 hit.
    [Graphical view]
    PROSITEiPS00022. EGF_1. 3 hits.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS01248. EGF_LAM_1. 2 hits.
    PS50027. EGF_LAM_2. 3 hits.
    PS51117. LAMININ_NTER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96CW9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLHLLALFLH CLPLASGDYD ICKSWVTTDE GPTWEFYACQ PKVMRLKDYV    50
    KVKVEPSGIT CGDPPERFCS HENPYLCSNE CDASNPDLAH PPRLMFDKEE 100
    EGLATYWQSI TWSRYPSPLE ANITLSWNKT VELTDDVVMT FEYGRPTVMV 150
    LEKSLDNGRT WQPYQFYAED CMEAFGMSAR RARDMSSSSA HRVLCTEEYS 200
    RWAGSKKEKH VRFEVRDRFA IFAGPDLRNM DNLYTRLESA KGLKEFFTLT 250
    DLRMRLLRPA LGGTYVQREN LYKYFYAISN IEVIGRCKCN LHANLCSMRE 300
    GSLQCECEHN TTGPDCGKCK KNFRTRSWRA GSYLPLPHGS PNACATAGSF 350
    GNCECYGHSN RCSYIDFLNV VTCVSCKHNT RGQHCQHCRL GYYRNGSAEL 400
    DDENVCIECN CNQIGSVHDR CNETGFCECR EGAAGPKCDD CLPTHYWRQG 450
    CYPNVCDDDQ LLCQNGGTCL QNQRCACPRG YTGVRCEQPR CDPADDDGGL 500
    DCDRAPGAAP RPATLLGCLL LLGLAARLGR 530
    Length:530
    Mass (Da):59,799
    Last modified:January 11, 2011 - v2
    Checksum:iB93A8A7E0E5A8CA3
    GO
    Isoform 2 (identifier: Q96CW9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         352-422: NCECYGHSNR...QIGSVHDRCN → TLQTPPPGRS...DPGGPWLGSQ
         423-530: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:422
    Mass (Da):47,491
    Checksum:i9A4B38913B097AF9
    GO

    Sequence cautioni

    The sequence BAB47486.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti346 – 3461T → A.3 Publications
    Corresponds to variant rs4962173 [ dbSNP | Ensembl ].
    VAR_047847

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei352 – 42271NCECY…HDRCN → TLQTPPPGRSPSALRGSRRG LANVKEPAGSRPQISEMLLG CTVTLHQGSVGPHIPPKLSL PDPGGPWLGSQ in isoform 2. 1 PublicationVSP_013147Add
    BLAST
    Alternative sequencei423 – 530108Missing in isoform 2. 1 PublicationVSP_013148Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB058760 mRNA. Translation: BAB47486.2. Different initiation.
    AY358165 mRNA. Translation: AAQ88532.1.
    AL353631, AL159997 Genomic DNA. Translation: CAI16125.1.
    AL159997, AL353631 Genomic DNA. Translation: CAI40856.1.
    BC013770 mRNA. Translation: AAH13770.1.
    CCDSiCCDS6946.1. [Q96CW9-1]
    RefSeqiNP_115925.2. NM_032536.2. [Q96CW9-1]
    UniGeneiHs.163642.

    Genome annotation databases

    EnsembliENST00000372179; ENSP00000361252; ENSG00000196358. [Q96CW9-2]
    ENST00000393229; ENSP00000376921; ENSG00000196358. [Q96CW9-1]
    GeneIDi84628.
    KEGGihsa:84628.
    UCSCiuc004cbh.2. human. [Q96CW9-1]

    Polymorphism databases

    DMDMi317373402.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB058760 mRNA. Translation: BAB47486.2 . Different initiation.
    AY358165 mRNA. Translation: AAQ88532.1 .
    AL353631 , AL159997 Genomic DNA. Translation: CAI16125.1 .
    AL159997 , AL353631 Genomic DNA. Translation: CAI40856.1 .
    BC013770 mRNA. Translation: AAH13770.1 .
    CCDSi CCDS6946.1. [Q96CW9-1 ]
    RefSeqi NP_115925.2. NM_032536.2. [Q96CW9-1 ]
    UniGenei Hs.163642.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TBD X-ray 1.80 A 18-349 [» ]
    3ZYG X-ray 2.20 A/B 1-345 [» ]
    3ZYI X-ray 2.60 B 1-345 [» ]
    ProteinModelPortali Q96CW9.
    SMRi Q96CW9. Positions 18-527.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124157. 2 interactions.
    IntActi Q96CW9. 4 interactions.
    MINTi MINT-7969778.
    STRINGi 9606.ENSP00000376921.

    PTM databases

    PhosphoSitei Q96CW9.

    Polymorphism databases

    DMDMi 317373402.

    Proteomic databases

    PaxDbi Q96CW9.
    PRIDEi Q96CW9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000372179 ; ENSP00000361252 ; ENSG00000196358 . [Q96CW9-2 ]
    ENST00000393229 ; ENSP00000376921 ; ENSG00000196358 . [Q96CW9-1 ]
    GeneIDi 84628.
    KEGGi hsa:84628.
    UCSCi uc004cbh.2. human. [Q96CW9-1 ]

    Organism-specific databases

    CTDi 84628.
    GeneCardsi GC09P135037.
    H-InvDB HIX0008489.
    HGNCi HGNC:14288. NTNG2.
    neXtProti NX_Q96CW9.
    PharmGKBi PA134962540.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG286598.
    HOGENOMi HOG000231614.
    HOVERGENi HBG052676.
    KOi K16359.
    OrthoDBi EOG7HQN7W.
    PhylomeDBi Q96CW9.
    TreeFami TF333945.

    Miscellaneous databases

    GeneWikii NTNG2.
    GenomeRNAii 84628.
    NextBioi 74534.
    PROi Q96CW9.

    Gene expression databases

    ArrayExpressi Q96CW9.
    Bgeei Q96CW9.
    CleanExi HS_NTNG2.
    Genevestigatori Q96CW9.

    Family and domain databases

    InterProi IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR002049. EGF_laminin.
    IPR008211. Laminin_N.
    [Graphical view ]
    Pfami PF00053. Laminin_EGF. 3 hits.
    PF00055. Laminin_N. 1 hit.
    [Graphical view ]
    SMARTi SM00181. EGF. 1 hit.
    SM00180. EGF_Lam. 3 hits.
    SM00136. LamNT. 1 hit.
    [Graphical view ]
    PROSITEi PS00022. EGF_1. 3 hits.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS01248. EGF_LAM_1. 2 hits.
    PS50027. EGF_LAM_2. 3 hits.
    PS51117. LAMININ_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
      DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-346.
      Tissue: BrainImported.
    2. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
      Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
      DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ALA-346.
    4. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-346.
      Tissue: Adrenal glandImported.
    6. "Structural basis for cell surface patterning through NetrinG-NGL interactions."
      Seiradake E., Coles C.H., Perestenko P.V., Harlos K., McIlhinney R.A., Aricescu A.R., Jones E.Y.
      EMBO J. 30:4479-4488(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-345 ALONE AND IN COMPLEX WITH LRRC4, FUNCTION, SUBCELLULAR LOCATION.
    7. "Crystal structure of the ligand binding domain of netrin G2."
      Brasch J., Harrison O.J., Ahlsen G., Liu Q., Shapiro L.
      J. Mol. Biol. 414:723-734(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 18-349, DISULFIDE BONDS, GLYCOSYLATION AT ASN-122 AND ASN-128.

    Entry informationi

    Entry nameiNTNG2_HUMAN
    AccessioniPrimary (citable) accession number: Q96CW9
    Secondary accession number(s): Q5JUJ2, Q6UXY0, Q96JH0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3