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Q96CW5 (GCP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gamma-tubulin complex component 3

Short name=GCP-3
Short name=hGCP3
Alternative name(s):
Gamma-ring complex protein 104 kDa
Short name=h104p
Short name=hGrip104
Spindle pole body protein Spc98 homolog
Short name=hSpc98
Gene names
Name:TUBGCP3
Synonyms:GCP3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length907 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.

Subunit structure

Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBGCP3 and CDK5RAP2. Ref.8

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome.

Tissue specificity

Ubiquitously expressed.

Sequence similarities

Belongs to the TUBGCP family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q96CW5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96CW5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     817-824: GQWGVTAA → VEMCLYCV
     825-907: Missing.
Isoform 3 (identifier: Q96CW5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     391-434: RKGGELASAV...PVLSFLYRWI → PTRVFPTHVF...WHSLCFRTRL
     435-907: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.12
Chain2 – 907906Gamma-tubulin complex component 3
PRO_0000078118

Regions

Compositional bias825 – 8295Poly-Glu

Amino acid modifications

Modified residue21N-acetylalanine Ref.12

Natural variations

Alternative sequence391 – 43444RKGGE…LYRWI → PTRVFPTHVFPTRDFPTRDF PMHVFPTRVFPTRVWHSLCF RTRL in isoform 3.
VSP_001622
Alternative sequence435 – 907473Missing in isoform 3.
VSP_001623
Alternative sequence817 – 8248GQWGVTAA → VEMCLYCV in isoform 2.
VSP_001620
Alternative sequence825 – 90783Missing in isoform 2.
VSP_001621
Natural variant2081T → S. Ref.2
Corresponds to variant rs1044287 [ dbSNP | Ensembl ].
VAR_049251

Experimental info

Sequence conflict3611S → I in CAA05832. Ref.2
Sequence conflict3611S → I in CAA05833. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 6, 2002. Version 2.
Checksum: 70FE2FDB7C80344D

FASTA907103,571
        10         20         30         40         50         60 
MATPDQKSPN VLLQNLCCRI LGRSEADVAQ QFQYAVRVIG SNFAPTVERD EFLVAEKIKK 

        70         80         90        100        110        120 
ELIRQRREAD AALFSELHRK LHSQGVLKNK WSILYLLLSL SEDPRRQPSK VSSYATLFAQ 

       130        140        150        160        170        180 
ALPRDAHSTP YYYARPQTLP LSYQDRSAQS AQSSGSVGSS GISSIGLCAL SGPAPAPQSL 

       190        200        210        220        230        240 
LPGQSNQAPG VGDCLRQQLG SRLAWTLTAN QPSSQATTSK GVPSAVSRNM TRSRREGDTG 

       250        260        270        280        290        300 
GTMEITEAAL VRDILYVFQG IDGKNIKMNN TENCYKVEGK ANLSRSLRDT AVRLSELGWL 

       310        320        330        340        350        360 
HNKIRRYTDQ RSLDRSFGLV GQSFCAALHQ ELREYYRLLS VLHSQLQLED DQGVNLGLES 

       370        380        390        400        410        420 
SLTLRRLLVW TYDPKIRLKT LAALVDHCQG RKGGELASAV HAYTKTGDPY MRSLVQHILS 

       430        440        450        460        470        480 
LVSHPVLSFL YRWIYDGELE DTYHEFFVAS DPTVKTDRLW HDKYTLRKSM IPSFMTMDQS 

       490        500        510        520        530        540 
RKVLLIGKSI NFLHQVCHDQ TPTTKMIAVT KSAESPQDAA DLFTDLENAF QGKIDAAYFE 

       550        560        570        580        590        600 
TSKYLLDVLN KKYSLLDHMQ AMRRYLLLGQ GDFIRHLMDL LKPELVRPAT TLYQHNLTGI 

       610        620        630        640        650        660 
LETAVRATNA QFDSPEILRR LDVRLLEVSP GDTGWDVFSL DYHVDGPIAT VFTRECMSHY 

       670        680        690        700        710        720 
LRVFNFLWRA KRMEYILTDI RKGHMCNAKL LRNMPEFSGV LHQCHILASE MVHFIHQMQY 

       730        740        750        760        770        780 
YITFEVLECS WDELWNKVQQ AQDLDHIIAA HEVFLDTIIS RCLLDSDSRA LLNQLRAVFD 

       790        800        810        820        830        840 
QIIELQNAQD AIYRAALEEL QRRLQFEEKK KQREIEGQWG VTAAEEEEEN KRIGEFKESI 

       850        860        870        880        890        900 
PKMCSQLRIL THFYQGIVQQ FLVLLTTSSD ESLRFLSFRL DFNEHYKARE PRLRVSLGTR 


GRRSSHT 

« Hide

Isoform 2 [UniParc].

Checksum: DF5AEDA7A34E531A
Show »

FASTA82493,885
Isoform 3 [UniParc].

Checksum: 9FB608525458BB54
Show »

FASTA43448,647

References

« Hide 'large scale' references
[1]"The mammalian gamma-tubulin complex contains homologues of the yeast spindle pole body components spc97p and spc98p."
Murphy S.M., Urbani L., Stearns T.
J. Cell Biol. 141:663-674(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Cervix carcinoma.
[2]"Characterization of the human homologue of the yeast spc98p and its association with gamma-tubulin."
Tassin A.-M., Celati C., Moudjou M., Bornens M.
J. Cell Biol. 141:689-701(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), CHARACTERIZATION, VARIANT SER-208.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thalamus.
[5]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Eye, Muscle and Testis.
[8]"CDK5RAP2 is a pericentriolar protein that functions in centrosomal attachment of the gamma-tubulin ring complex."
Fong K.W., Choi Y.K., Rattner J.B., Qi R.Z.
Mol. Biol. Cell 19:115-125(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CDK5RAP2.
[9]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF042378 mRNA. Translation: AAC39727.1.
AJ003061 mRNA. Translation: CAA05832.1.
AJ003062 mRNA. Translation: CAA05833.1.
BT009772 mRNA. Translation: AAP88774.1.
AK314785 mRNA. Translation: BAG37319.1.
AL139384, AL160033 Genomic DNA. Translation: CAI16949.1.
AL139384, AL160033 Genomic DNA. Translation: CAI16950.1.
AL160033, AL139384 Genomic DNA. Translation: CAI14455.1.
AL160033, AL139384 Genomic DNA. Translation: CAI14456.1.
CH471085 Genomic DNA. Translation: EAX09164.1.
BC007763 mRNA. Translation: AAH07763.1.
BC013781 mRNA. Translation: AAH13781.1.
BC046634 mRNA. Translation: AAH46634.1.
RefSeqNP_001273206.1. NM_001286277.1.
NP_001273207.1. NM_001286278.1.
NP_001273208.1. NM_001286279.1.
NP_006313.1. NM_006322.5.
UniGeneHs.224152.

3D structure databases

ProteinModelPortalQ96CW5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115695. 21 interactions.
IntActQ96CW5. 20 interactions.
MINTMINT-3051799.
STRING9606.ENSP00000261965.

PTM databases

PhosphoSiteQ96CW5.

Polymorphism databases

DMDM21362575.

Proteomic databases

PaxDbQ96CW5.
PRIDEQ96CW5.

Protocols and materials databases

DNASU10426.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261965; ENSP00000261965; ENSG00000126216. [Q96CW5-1]
ENST00000375669; ENSP00000364821; ENSG00000126216. [Q96CW5-2]
GeneID10426.
KEGGhsa:10426.
UCSCuc001vse.1. human. [Q96CW5-1]
uc001vsf.3. human. [Q96CW5-2]
uc001vsg.1. human. [Q96CW5-3]

Organism-specific databases

CTD10426.
GeneCardsGC13M113139.
HGNCHGNC:18598. TUBGCP3.
HPAHPA043913.
neXtProtNX_Q96CW5.
PharmGKBPA38597.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG309182.
HOGENOMHOG000007959.
HOVERGENHBG031568.
InParanoidQ96CW5.
KOK16570.
OMAHCHILAS.
OrthoDBEOG7FFMR6.
PhylomeDBQ96CW5.
TreeFamTF300705.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.

Gene expression databases

BgeeQ96CW5.
CleanExHS_TUBGCP3.
GenevestigatorQ96CW5.

Family and domain databases

InterProIPR015697. GCP3.
IPR007259. TUBGCP.
[Graphical view]
PANTHERPTHR19302. PTHR19302. 1 hit.
PTHR19302:SF14. PTHR19302:SF14. 1 hit.
PfamPF04130. Spc97_Spc98. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTUBGCP3. human.
GeneWikiTUBGCP3.
GenomeRNAi10426.
NextBio39516.
PROQ96CW5.

Entry information

Entry nameGCP3_HUMAN
AccessionPrimary (citable) accession number: Q96CW5
Secondary accession number(s): O43631 expand/collapse secondary AC list , O60852, O60853, Q5T8L2, Q7Z4K1, Q96I79
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM