Q96CW5 (GCP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-tubulin complex component 3
Short name=GCP-3
Short name=hGCP3

Alternative name(s):
Gamma-ring complex protein 104 kDa
Short name=h104p
Short name=hGrip104
Spindle pole body protein Spc98 homolog
Short name=hSpc98

Gene names

Name:	TUBGCP3
Synonyms:	GCP3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	907 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
Subunit structure	Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBGCP3 and CDK5RAP2. Ref.8
Subcellular location	Cytoplasm › cytoskeleton › centrosome.
Tissue specificity	Ubiquitously expressed.
Sequence similarities	Belongs to the TUBGCP family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing Polymorphism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Traceable author statement. Source: Reactome microtubule nucleation Non-traceable author statement PubMed 11694571Ref.2. Source: UniProtKB single fertilization Non-traceable author statement PubMed 9138452. Source: UniProtKB
Cellular_component	centriole Inferred from direct assay Ref.2. Source: UniProtKB cytosol Traceable author statement. Source: Reactome polar microtubule Inferred from direct assay Ref.2. Source: UniProtKB
Molecular_function	gamma-tubulin binding Inferred from direct assay Ref.2. Source: UniProtKB structural constituent of cytoskeleton Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q96CW5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96CW5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     817-824: GQWGVTAA → VEMCLYCV
     825-907: Missing.

Isoform 3 (identifier: Q96CW5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     391-434: RKGGELASAV...PVLSFLYRWI → PTRVFPTHVF...WHSLCFRTRL
     435-907: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 907	907	Gamma-tubulin complex component 3		PRO_0000078118
Regions
	Compositional bias	825 – 829	5	Poly-Glu
Natural variations
	Alternative sequence	391 – 434	44	RKGGE…LYRWI → PTRVFPTHVFPTRDFPTRDF PMHVFPTRVFPTRVWHSLCF RTRL in isoform 3.		VSP_001622
	Alternative sequence	435 – 907	473	Missing in isoform 3.		VSP_001623
	Alternative sequence	817 – 824	8	GQWGVTAA → VEMCLYCV in isoform 2.		VSP_001620
	Alternative sequence	825 – 907	83	Missing in isoform 2.		VSP_001621
	Natural variant	208	1	T → S. Ref.2 Corresponds to variant rs1044287 [ dbSNP | Ensembl ].		VAR_049251
Experimental info
	Sequence conflict	361	1	S → I in CAA05832. Ref.2
	Sequence conflict	361	1	S → I in CAA05833. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: 70FE2FDB7C80344D	FASTA	907	103,571
	10 20 30 40 50 60 MATPDQKSPN VLLQNLCCRI LGRSEADVAQ QFQYAVRVIG SNFAPTVERD EFLVAEKIKK 70 80 90 100 110 120 ELIRQRREAD AALFSELHRK LHSQGVLKNK WSILYLLLSL SEDPRRQPSK VSSYATLFAQ 130 140 150 160 170 180 ALPRDAHSTP YYYARPQTLP LSYQDRSAQS AQSSGSVGSS GISSIGLCAL SGPAPAPQSL 190 200 210 220 230 240 LPGQSNQAPG VGDCLRQQLG SRLAWTLTAN QPSSQATTSK GVPSAVSRNM TRSRREGDTG 250 260 270 280 290 300 GTMEITEAAL VRDILYVFQG IDGKNIKMNN TENCYKVEGK ANLSRSLRDT AVRLSELGWL 310 320 330 340 350 360 HNKIRRYTDQ RSLDRSFGLV GQSFCAALHQ ELREYYRLLS VLHSQLQLED DQGVNLGLES 370 380 390 400 410 420 SLTLRRLLVW TYDPKIRLKT LAALVDHCQG RKGGELASAV HAYTKTGDPY MRSLVQHILS 430 440 450 460 470 480 LVSHPVLSFL YRWIYDGELE DTYHEFFVAS DPTVKTDRLW HDKYTLRKSM IPSFMTMDQS 490 500 510 520 530 540 RKVLLIGKSI NFLHQVCHDQ TPTTKMIAVT KSAESPQDAA DLFTDLENAF QGKIDAAYFE 550 560 570 580 590 600 TSKYLLDVLN KKYSLLDHMQ AMRRYLLLGQ GDFIRHLMDL LKPELVRPAT TLYQHNLTGI 610 620 630 640 650 660 LETAVRATNA QFDSPEILRR LDVRLLEVSP GDTGWDVFSL DYHVDGPIAT VFTRECMSHY 670 680 690 700 710 720 LRVFNFLWRA KRMEYILTDI RKGHMCNAKL LRNMPEFSGV LHQCHILASE MVHFIHQMQY 730 740 750 760 770 780 YITFEVLECS WDELWNKVQQ AQDLDHIIAA HEVFLDTIIS RCLLDSDSRA LLNQLRAVFD 790 800 810 820 830 840 QIIELQNAQD AIYRAALEEL QRRLQFEEKK KQREIEGQWG VTAAEEEEEN KRIGEFKESI 850 860 870 880 890 900 PKMCSQLRIL THFYQGIVQQ FLVLLTTSSD ESLRFLSFRL DFNEHYKARE PRLRVSLGTR GRRSSHT
	Isoform 2 [UniParc]. Checksum: DF5AEDA7A34E531A	FASTA	824	93,885
	10 20 30 40 50 60 MATPDQKSPN VLLQNLCCRI LGRSEADVAQ QFQYAVRVIG SNFAPTVERD EFLVAEKIKK 70 80 90 100 110 120 ELIRQRREAD AALFSELHRK LHSQGVLKNK WSILYLLLSL SEDPRRQPSK VSSYATLFAQ 130 140 150 160 170 180 ALPRDAHSTP YYYARPQTLP LSYQDRSAQS AQSSGSVGSS GISSIGLCAL SGPAPAPQSL 190 200 210 220 230 240 LPGQSNQAPG VGDCLRQQLG SRLAWTLTAN QPSSQATTSK GVPSAVSRNM TRSRREGDTG 250 260 270 280 290 300 GTMEITEAAL VRDILYVFQG IDGKNIKMNN TENCYKVEGK ANLSRSLRDT AVRLSELGWL 310 320 330 340 350 360 HNKIRRYTDQ RSLDRSFGLV GQSFCAALHQ ELREYYRLLS VLHSQLQLED DQGVNLGLES 370 380 390 400 410 420 SLTLRRLLVW TYDPKIRLKT LAALVDHCQG RKGGELASAV HAYTKTGDPY MRSLVQHILS 430 440 450 460 470 480 LVSHPVLSFL YRWIYDGELE DTYHEFFVAS DPTVKTDRLW HDKYTLRKSM IPSFMTMDQS 490 500 510 520 530 540 RKVLLIGKSI NFLHQVCHDQ TPTTKMIAVT KSAESPQDAA DLFTDLENAF QGKIDAAYFE 550 560 570 580 590 600 TSKYLLDVLN KKYSLLDHMQ AMRRYLLLGQ GDFIRHLMDL LKPELVRPAT TLYQHNLTGI 610 620 630 640 650 660 LETAVRATNA QFDSPEILRR LDVRLLEVSP GDTGWDVFSL DYHVDGPIAT VFTRECMSHY 670 680 690 700 710 720 LRVFNFLWRA KRMEYILTDI RKGHMCNAKL LRNMPEFSGV LHQCHILASE MVHFIHQMQY 730 740 750 760 770 780 YITFEVLECS WDELWNKVQQ AQDLDHIIAA HEVFLDTIIS RCLLDSDSRA LLNQLRAVFD 790 800 810 820 QIIELQNAQD AIYRAALEEL QRRLQFEEKK KQREIEVEMC LYCV
	Isoform 3 [UniParc]. Checksum: 9FB608525458BB54	FASTA	434	48,647
	10 20 30 40 50 60 MATPDQKSPN VLLQNLCCRI LGRSEADVAQ QFQYAVRVIG SNFAPTVERD EFLVAEKIKK 70 80 90 100 110 120 ELIRQRREAD AALFSELHRK LHSQGVLKNK WSILYLLLSL SEDPRRQPSK VSSYATLFAQ 130 140 150 160 170 180 ALPRDAHSTP YYYARPQTLP LSYQDRSAQS AQSSGSVGSS GISSIGLCAL SGPAPAPQSL 190 200 210 220 230 240 LPGQSNQAPG VGDCLRQQLG SRLAWTLTAN QPSSQATTSK GVPSAVSRNM TRSRREGDTG 250 260 270 280 290 300 GTMEITEAAL VRDILYVFQG IDGKNIKMNN TENCYKVEGK ANLSRSLRDT AVRLSELGWL 310 320 330 340 350 360 HNKIRRYTDQ RSLDRSFGLV GQSFCAALHQ ELREYYRLLS VLHSQLQLED DQGVNLGLES 370 380 390 400 410 420 SLTLRRLLVW TYDPKIRLKT LAALVDHCQG PTRVFPTHVF PTRDFPTRDF PMHVFPTRVF 430 PTRVWHSLCF RTRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mammalian gamma-tubulin complex contains homologues of the yeast spindle pole body components spc97p and spc98p." Murphy S.M., Urbani L., Stearns T. J. Cell Biol. 141:663-674(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Cervix carcinoma.
[2]	"Characterization of the human homologue of the yeast spc98p and its association with gamma-tubulin." Tassin A.-M., Celati C., Moudjou M., Bornens M. J. Cell Biol. 141:689-701(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), CHARACTERIZATION, VARIANT SER-208.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus.
[5]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Eye, Muscle and Testis.
[8]	"CDK5RAP2 is a pericentriolar protein that functions in centrosomal attachment of the gamma-tubulin ring complex." Fong K.W., Choi Y.K., Rattner J.B., Qi R.Z. Mol. Biol. Cell 19:115-125(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CDK5RAP2.
[9]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF042378 mRNA. Translation: AAC39727.1. AJ003061 mRNA. Translation: CAA05832.1. AJ003062 mRNA. Translation: CAA05833.1. BT009772 mRNA. Translation: AAP88774.1. AK314785 mRNA. Translation: BAG37319.1. AL139384, AL160033 Genomic DNA. Translation: CAI16949.1. AL139384, AL160033 Genomic DNA. Translation: CAI16950.1. AL160033, AL139384 Genomic DNA. Translation: CAI14455.1. AL160033, AL139384 Genomic DNA. Translation: CAI14456.1. CH471085 Genomic DNA. Translation: EAX09164.1. BC007763 mRNA. Translation: AAH07763.1. BC013781 mRNA. Translation: AAH13781.1. BC046634 mRNA. Translation: AAH46634.1.
IPI	IPI00033516. IPI00221175. IPI00399300.
RefSeq	NP_006313.1. NM_006322.4.
UniGene	Hs.224152. Hs.595145.
3D structure databases
ProteinModelPortal	Q96CW5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96CW5. 17 interactions.
STRING	9606.ENSP00000261965.
PTM databases
PhosphoSite	Q96CW5.
Polymorphism databases
DMDM	21362575.
Proteomic databases
PaxDb	Q96CW5.
PRIDE	Q96CW5.
Protocols and materials databases
DNASU	10426.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261965; ENSP00000261965; ENSG00000126216. ENST00000375669; ENSP00000364821; ENSG00000126216.
GeneID	10426.
KEGG	hsa:10426.
UCSC	uc001vse.1. human. uc001vsf.3. human. uc001vsg.1. human.
Organism-specific databases
CTD	10426.
GeneCards	GC13M113139.
HGNC	HGNC:18598. TUBGCP3.
HPA	HPA043913.
neXtProt	NX_Q96CW5.
PharmGKB	PA38597.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG309182.
HOGENOM	HOG000007959.
HOVERGEN	HBG031568.
InParanoid	Q96CW5.
KO	K16570.
OMA	HCHILAS.
OrthoDB	EOG4Z0B4X.
PhylomeDB	Q96CW5.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle.
Gene expression databases
ArrayExpress	Q96CW5.
Bgee	Q96CW5.
CleanEx	HS_TUBGCP3.
Genevestigator	Q96CW5.
GermOnline	ENSG00000126216. Homo sapiens.
Family and domain databases
InterPro	IPR015697. GCP3. IPR007259. Spc97_Spc98. [Graphical view]
PANTHER	PTHR19302. PTHR19302. 1 hit. PTHR19302:SF14. PTHR19302:SF14. 1 hit.
Pfam	PF04130. Spc97_Spc98. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TUBGCP3. human.
GenomeRNAi	10426.
NextBio	39516.

Entry information

Entry name

GCP3_HUMAN

Accession

Primary (citable) accession number: Q96CW5
Secondary accession number(s): O43631 , O60852, O60853, Q5T8L2, Q7Z4K1, Q96I79

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	June 6, 2002
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.