Q96CS7 (PKHB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pleckstrin homology domain-containing family B member 2
Short name=PH domain-containing family B member 2

Alternative name(s):
Evectin-2

Gene names

Name:	PLEKHB2
Synonyms:	EVT2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in retrograde transport of recycling endosomes. Ref.6 Ref.7
Subcellular location	Recycling endosome membrane; Peripheral membrane protein. Note: Specifically detected in tubulovesicular structures, and colocalizes with TFNR. Ref.6 Ref.7
Domain	The PH domain specifically binds phosphatidylserine, which is enriched in recycling endosome membranes, it doesn't recognize PIPs.
Sequence similarities	Contains 1 PH domain.

Ontologies

Keywords
Cellular component	Endosome Membrane
Coding sequence diversity	Alternative splicing
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	recycling endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96CS7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96CS7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     142-222: QAYGYGPYGG...MALGSLFWVF → ELHSKGPKIH...PPPARPPALR

Note: No experimental confirmation available.

Isoform 3 (identifier: Q96CS7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     142-142: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q96CS7-4)

The sequence of this isoform differs from the canonical sequence as follows:
     99-222: AWKFTLQDSR...MALGSLFWVF → LMAMGHTVVR...CWQEQPRAWP

Isoform 5 (identifier: Q96CS7-5)

The sequence of this isoform differs from the canonical sequence as follows:
     142-222: QAYGYGPYGG...MALGSLFWVF → DFMDSSLLTK...CWQEQPRAWP

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 222

222

Pleckstrin homology domain-containing family B member 2

PRO_0000053889

Regions

Domain

2 – 109

108

PH

Sites

Binding site

20

1

Phosphatidylserine

Natural variations

Alternative sequence

99 – 222

124

AWKFT…LFWVF → LMAMGHTVVRTRQELKLSTL RMGRRMPCPTSTHMQDFMDS SLLTKSSFESAIETTTATWH WACWQEQPRAWP in isoform 4.

VSP_045163

Alternative sequence

142 – 222

81

QAYGY…LFWVF → ELHSKGPKIHTTTSAKTQAV LPTRCFFPSESPIRRSVQIS VLLPAVNIRRRIKLTFPQPA RPRAPRSLCLWQTWFAGKRA PSLPTAATAPALPSCPSPPA PSKALPPSLAGWRSPSSAPL PPEQALAPRSFQLSKHQARP RPHRPGTPPVPAWPGVPPPP PARPPALR in isoform 2.

VSP_009783

Alternative sequence

142 – 222

81

QAYGY…LFWVF → DFMDSSLLTKSSFESAIETT TATWHWACWQEQPRAWP in isoform 5.

VSP_045164

Alternative sequence

142

1

Missing in isoform 3.

VSP_009784

Experimental info

Sequence conflict

172

1

Y → H in BAA91379. Ref.1

Secondary structure

1

.

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222

Helix Strand Turn

Details...

Beta strand

4 – 12

9

Beta strand

14 – 16

3

Beta strand

19 – 26

8

Beta strand

30 – 37

8

Beta strand

42 – 48

7

Helix

49 – 52

4

Beta strand

53 – 58

6

Helix

59 – 61

3

Helix

73 – 75

3

Beta strand

76 – 81

6

Beta strand

86 – 90

5

Helix

94 – 108

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 8924F61890D416A5	FASTA	222	24,736
	10 20 30 40 50 60 MAFVKSGWLL RQSTILKRWK KNWFDLWSDG HLIYYDDQTR QNIEDKVHMP MDCINIRTGQ 70 80 90 100 110 120 ECRDTQPPDG KSKDCMLQIV CRDGKTISLC AESTDDCLAW KFTLQDSRTN TAYVGSAVMT 130 140 150 160 170 180 DETSVVSSPP PYTAYAAPAP EQAYGYGPYG GAYPPGTQVV YAANGQAYAV PYQYPYAGLY 190 200 210 220 GQQPANQVII RERYRDNDSD LALGMLAGAA TGMALGSLFW VF
	Isoform 2 [UniParc]. Checksum: BB64D755DBDBD100	FASTA	309	34,154
	10 20 30 40 50 60 MAFVKSGWLL RQSTILKRWK KNWFDLWSDG HLIYYDDQTR QNIEDKVHMP MDCINIRTGQ 70 80 90 100 110 120 ECRDTQPPDG KSKDCMLQIV CRDGKTISLC AESTDDCLAW KFTLQDSRTN TAYVGSAVMT 130 140 150 160 170 180 DETSVVSSPP PYTAYAAPAP EELHSKGPKI HTTTSAKTQA VLPTRCFFPS ESPIRRSVQI 190 200 210 220 230 240 SVLLPAVNIR RRIKLTFPQP ARPRAPRSLC LWQTWFAGKR APSLPTAATA PALPSCPSPP 250 260 270 280 290 300 APSKALPPSL AGWRSPSSAP LPPEQALAPR SFQLSKHQAR PRPHRPGTPP VPAWPGVPPP PPARPPALR
	Isoform 3 [UniParc]. Checksum: 6740C7F3A491AB09	FASTA	221	24,608
	10 20 30 40 50 60 MAFVKSGWLL RQSTILKRWK KNWFDLWSDG HLIYYDDQTR QNIEDKVHMP MDCINIRTGQ 70 80 90 100 110 120 ECRDTQPPDG KSKDCMLQIV CRDGKTISLC AESTDDCLAW KFTLQDSRTN TAYVGSAVMT 130 140 150 160 170 180 DETSVVSSPP PYTAYAAPAP EAYGYGPYGG AYPPGTQVVY AANGQAYAVP YQYPYAGLYG 190 200 210 220 QQPANQVIIR ERYRDNDSDL ALGMLAGAAT GMALGSLFWV F
	Isoform 4 [UniParc]. Checksum: 70B6C31C4BD6AA19	FASTA	170	19,852
	10 20 30 40 50 60 MAFVKSGWLL RQSTILKRWK KNWFDLWSDG HLIYYDDQTR QNIEDKVHMP MDCINIRTGQ 70 80 90 100 110 120 ECRDTQPPDG KSKDCMLQIV CRDGKTISLC AESTDDCLLM AMGHTVVRTR QELKLSTLRM 130 140 150 160 170 GRRMPCPTST HMQDFMDSSL LTKSSFESAI ETTTATWHWA CWQEQPRAWP
	Isoform 5 [UniParc]. Checksum: 7903B0346AB5F5C0	FASTA	178	20,375
	10 20 30 40 50 60 MAFVKSGWLL RQSTILKRWK KNWFDLWSDG HLIYYDDQTR QNIEDKVHMP MDCINIRTGQ 70 80 90 100 110 120 ECRDTQPPDG KSKDCMLQIV CRDGKTISLC AESTDDCLAW KFTLQDSRTN TAYVGSAVMT 130 140 150 160 170 DETSVVSSPP PYTAYAAPAP EDFMDSSLLT KSSFESAIET TTATWHWACW QEQPRAWP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3; 4 AND 5). Tissue: Cerebellum, Colon, Lung and Testis.
[2]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Pancreas and Skin.
[6]	"Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes." Uchida Y., Hasegawa J., Chinnapen D., Inoue T., Okazaki S., Kato R., Wakatsuki S., Misaki R., Koike M., Uchiyama Y., Iemura S., Natsume T., Kuwahara R., Nakagawa T., Nishikawa K., Mukai K., Miyoshi E., Taniguchi N. , Sheff D., Lencer W.I., Taguchi T., Arai H. Proc. Natl. Acad. Sci. U.S.A. 108:15846-15851(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.0 ANGSTROMS) OF 1-110, FUNCTION, SUBCELLULAR LOCATION.
[7]	"Structural basis of the strict phospholipid binding specificity of the pleckstrin homology domain of human evectin-2." Okazaki S., Kato R., Uchida Y., Taguchi T., Arai H., Wakatsuki S. Acta Crystallogr. D 68:117-123(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 1-110 IN COMPLEX WITH O-PHOSPHOSERINE, FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK000790 mRNA. Translation: BAA91379.1.
AK123710 mRNA. Translation: BAG53942.1.
AK293880 mRNA. Translation: BAG57269.1.
AK302770 mRNA. Translation: BAG63978.1.
AK223338 mRNA. Translation: BAD97058.1.
AC073869 Genomic DNA. No translation available.
AC131180 Genomic DNA. No translation available.
AC009477 Genomic DNA. Translation: AAX93135.1.
CH471250 Genomic DNA. Translation: EAW51261.1.
CH471250 Genomic DNA. Translation: EAW51263.1.
BC001428 mRNA. Translation: AAH01428.1.
BC013991 mRNA. Translation: AAH13991.1.
BC050695 mRNA. Translation: AAH50695.2.

IPI

IPI00031228.
IPI00335457.
IPI00409715.

RefSeq

NP_001094093.1. NM_001100623.1.
NP_001253991.1. NM_001267062.1.
NP_001253992.1. NM_001267063.1.
NP_001253993.1. NM_001267064.1.
NP_001253994.1. NM_001267065.1.
NP_001253995.1. NM_001267066.1.
NP_001253996.1. NM_001267067.1.
NP_001253997.1. NM_001267068.1.
NP_060428.2. NM_017958.2.

UniGene

Hs.469944.
Hs.708368.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3AJ4	X-ray	1.00	A/B	1-110	[»]
3VIA	X-ray	1.75	A/B	1-110	[»]

ProteinModelPortal

Q96CS7.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96CS7. 2 interactions.

MINT

MINT-1180681.

STRING

9606.ENSP00000385892.

PTM databases

PhosphoSite

Q96CS7.

Polymorphism databases

DMDM

48474928.

Proteomic databases

PaxDb

Q96CS7.

PRIDE

Q96CS7.

Protocols and materials databases

DNASU

55041.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000234115; ENSP00000234115; ENSG00000115762.
ENST00000403716; ENSP00000385892; ENSG00000115762.
ENST00000409279; ENSP00000386666; ENSG00000115762.
ENST00000409612; ENSP00000386662; ENSG00000115762.
ENST00000438882; ENSP00000401193; ENSG00000115762.
ENST00000439822; ENSP00000389629; ENSG00000115762.

GeneID

55041.

KEGG

hsa:55041.

UCSC

uc002tsg.4. human.
uc002tsj.4. human.
uc010zap.2. human.
uc010zaq.2. human.

Organism-specific databases

CTD

55041.

GeneCards

GC02P131862.

HGNC

HGNC:19236. PLEKHB2.

neXtProt

NX_Q96CS7.

PharmGKB

PA134885873.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG42839.

HOGENOM

HOG000253945.

HOVERGEN

HBG060487.

Gene expression databases

ArrayExpress

Q96CS7.

Bgee

Q96CS7.

CleanEx

HS_PLEKHB2.

Genevestigator

Q96CS7.

GermOnline

ENSG00000115762. Homo sapiens.

Family and domain databases

Gene3D

2.30.29.30. 1 hit.

InterPro

IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]

Pfam

PF00169. PH. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

PLEKHB2. human.

GenomeRNAi

55041.

NextBio

58499.

Entry information

Entry name

PKHB2_HUMAN

Accession

Primary (citable) accession number: Q96CS7
Secondary accession number(s): B4DF08 , B4DZ66, Q53FF1, Q53TH7, Q86W37, Q9BV75, Q9NWK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.