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Q96CS7 (PKHB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pleckstrin homology domain-containing family B member 2

Short name=PH domain-containing family B member 2
Alternative name(s):
Evectin-2
Gene names
Name:PLEKHB2
Synonyms:EVT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in retrograde transport of recycling endosomes. Ref.6 Ref.7

Subcellular location

Recycling endosome membrane; Peripheral membrane protein. Note: Specifically detected in tubulovesicular structures, and colocalizes with TFNR. Ref.6 Ref.7

Domain

The PH domain specifically binds phosphatidylserine, which is enriched in recycling endosome membranes, it doesn't recognize PIPs.

Sequence similarities

Contains 1 PH domain.

Ontologies

Keywords
   Cellular componentEndosome
Membrane
   Coding sequence diversityAlternative splicing
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentrecycling endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96CS7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96CS7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     142-222: QAYGYGPYGG...MALGSLFWVF → ELHSKGPKIH...PPPARPPALR
Note: No experimental confirmation available.
Isoform 3 (identifier: Q96CS7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     142-142: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q96CS7-4)

The sequence of this isoform differs from the canonical sequence as follows:
     99-222: AWKFTLQDSR...MALGSLFWVF → LMAMGHTVVR...CWQEQPRAWP
Isoform 5 (identifier: Q96CS7-5)

The sequence of this isoform differs from the canonical sequence as follows:
     142-222: QAYGYGPYGG...MALGSLFWVF → DFMDSSLLTK...CWQEQPRAWP
Isoform 6 (identifier: Q96CS7-6)

The sequence of this isoform differs from the canonical sequence as follows:
     141-141: E → EVGRTLSLQ
Note: Gene prediction based on EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 222222Pleckstrin homology domain-containing family B member 2
PRO_0000053889

Regions

Domain2 – 109108PH

Sites

Binding site201Phosphatidylserine

Natural variations

Alternative sequence99 – 222124AWKFT…LFWVF → LMAMGHTVVRTRQELKLSTL RMGRRMPCPTSTHMQDFMDS SLLTKSSFESAIETTTATWH WACWQEQPRAWP in isoform 4.
VSP_045163
Alternative sequence1411E → EVGRTLSLQ in isoform 6.
VSP_047118
Alternative sequence142 – 22281QAYGY…LFWVF → ELHSKGPKIHTTTSAKTQAV LPTRCFFPSESPIRRSVQIS VLLPAVNIRRRIKLTFPQPA RPRAPRSLCLWQTWFAGKRA PSLPTAATAPALPSCPSPPA PSKALPPSLAGWRSPSSAPL PPEQALAPRSFQLSKHQARP RPHRPGTPPVPAWPGVPPPP PARPPALR in isoform 2.
VSP_009783
Alternative sequence142 – 22281QAYGY…LFWVF → DFMDSSLLTKSSFESAIETT TATWHWACWQEQPRAWP in isoform 5.
VSP_045164
Alternative sequence1421Missing in isoform 3.
VSP_009784

Experimental info

Sequence conflict1721Y → H in BAA91379. Ref.1

Secondary structure

..................... 222
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 8924F61890D416A5

FASTA22224,736
        10         20         30         40         50         60 
MAFVKSGWLL RQSTILKRWK KNWFDLWSDG HLIYYDDQTR QNIEDKVHMP MDCINIRTGQ 

        70         80         90        100        110        120 
ECRDTQPPDG KSKDCMLQIV CRDGKTISLC AESTDDCLAW KFTLQDSRTN TAYVGSAVMT 

       130        140        150        160        170        180 
DETSVVSSPP PYTAYAAPAP EQAYGYGPYG GAYPPGTQVV YAANGQAYAV PYQYPYAGLY 

       190        200        210        220 
GQQPANQVII RERYRDNDSD LALGMLAGAA TGMALGSLFW VF 

« Hide

Isoform 2 [UniParc].

Checksum: BB64D755DBDBD100
Show »

FASTA30934,154
Isoform 3 [UniParc].

Checksum: 6740C7F3A491AB09
Show »

FASTA22124,608
Isoform 4 [UniParc].

Checksum: 70B6C31C4BD6AA19
Show »

FASTA17019,852
Isoform 5 [UniParc].

Checksum: 7903B0346AB5F5C0
Show »

FASTA17820,375
Isoform 6 [UniParc].

Checksum: 08562F08D30AEE66
Show »

FASTA23025,591

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3; 4 AND 5).
Tissue: Cerebellum, Colon, Lung and Testis.
[2]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Testis.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Pancreas and Skin.
[6]"Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes."
Uchida Y., Hasegawa J., Chinnapen D., Inoue T., Okazaki S., Kato R., Wakatsuki S., Misaki R., Koike M., Uchiyama Y., Iemura S., Natsume T., Kuwahara R., Nakagawa T., Nishikawa K., Mukai K., Miyoshi E., Taniguchi N. expand/collapse author list , Sheff D., Lencer W.I., Taguchi T., Arai H.
Proc. Natl. Acad. Sci. U.S.A. 108:15846-15851(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.0 ANGSTROMS) OF 1-110, FUNCTION, SUBCELLULAR LOCATION.
[7]"Structural basis of the strict phospholipid binding specificity of the pleckstrin homology domain of human evectin-2."
Okazaki S., Kato R., Uchida Y., Taguchi T., Arai H., Wakatsuki S.
Acta Crystallogr. D 68:117-123(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 1-110 IN COMPLEX WITH O-PHOSPHOSERINE, FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK000790 mRNA. Translation: BAA91379.1.
AK123710 mRNA. Translation: BAG53942.1.
AK293880 mRNA. Translation: BAG57269.1.
AK302770 mRNA. Translation: BAG63978.1.
AK223338 mRNA. Translation: BAD97058.1.
AC073869 Genomic DNA. No translation available.
AC131180 Genomic DNA. No translation available.
AC009477 Genomic DNA. Translation: AAX93135.1.
CH471250 Genomic DNA. Translation: EAW51261.1.
CH471250 Genomic DNA. Translation: EAW51263.1.
CH471250 Genomic DNA. Translation: EAW51266.1.
BC001428 mRNA. Translation: AAH01428.1.
BC013991 mRNA. Translation: AAH13991.1.
BC050695 mRNA. Translation: AAH50695.2.
RefSeqNP_001094093.1. NM_001100623.1.
NP_001253991.1. NM_001267062.1.
NP_001253992.1. NM_001267063.1.
NP_001253993.1. NM_001267064.1.
NP_001253994.1. NM_001267065.1.
NP_001253995.1. NM_001267066.1.
NP_001253996.1. NM_001267067.1.
NP_001253997.1. NM_001267068.1.
NP_060428.2. NM_017958.2.
UniGeneHs.469944.
Hs.708368.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3AJ4X-ray1.00A/B1-110[»]
3VIAX-ray1.75A/B1-110[»]
ProteinModelPortalQ96CS7.
SMRQ96CS7. Positions 1-110.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120370. 8 interactions.
IntActQ96CS7. 3 interactions.
MINTMINT-1180681.
STRING9606.ENSP00000385892.

PTM databases

PhosphoSiteQ96CS7.

Polymorphism databases

DMDM48474928.

Proteomic databases

PaxDbQ96CS7.
PRIDEQ96CS7.

Protocols and materials databases

DNASU55041.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000234115; ENSP00000234115; ENSG00000115762. [Q96CS7-3]
ENST00000403716; ENSP00000385892; ENSG00000115762. [Q96CS7-1]
ENST00000409158; ENSP00000386410; ENSG00000115762. [Q96CS7-6]
ENST00000409279; ENSP00000386666; ENSG00000115762. [Q96CS7-1]
ENST00000409612; ENSP00000386662; ENSG00000115762. [Q96CS7-1]
ENST00000438882; ENSP00000401193; ENSG00000115762. [Q96CS7-5]
ENST00000439822; ENSP00000389629; ENSG00000115762. [Q96CS7-4]
GeneID55041.
KEGGhsa:55041.
UCSCuc002tsg.4. human. [Q96CS7-1]
uc002tsj.4. human. [Q96CS7-3]
uc010zap.2. human.
uc010zaq.2. human.

Organism-specific databases

CTD55041.
GeneCardsGC02P131862.
HGNCHGNC:19236. PLEKHB2.
neXtProtNX_Q96CS7.
PharmGKBPA134885873.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42839.
HOGENOMHOG000253945.
HOVERGENHBG060487.
OMACINIRMG.
OrthoDBEOG7CZK73.
PhylomeDBQ96CS7.
TreeFamTF331787.

Gene expression databases

ArrayExpressQ96CS7.
BgeeQ96CS7.
CleanExHS_PLEKHB2.
GenevestigatorQ96CS7.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PfamPF00169. PH. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
[Graphical view]
PROSITEPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPLEKHB2. human.
GeneWikiPLEKHB2.
GenomeRNAi55041.
NextBio58499.
PROQ96CS7.

Entry information

Entry namePKHB2_HUMAN
AccessionPrimary (citable) accession number: Q96CS7
Secondary accession number(s): B4DF08 expand/collapse secondary AC list , B4DZ66, B8ZZN1, Q53FF1, Q53TH7, Q86W37, Q9BV75, Q9NWK1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM