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Protein

Solute carrier family 25 member 36

Gene

SLC25A36

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial transporter that imports/exports pyrimidine nucleotides into and from mitochondria. Transports preferentially cytosine and uracil (deoxy)nucleoside mono-, di-, and triphosphates by uniport and antiport mechanism. Also transports guanine but not adenine (deoxy)nucleotides. Is inhibited strongly by pyridoxal 5'-phosphate, 4,7-diphenyl-1,10-phenanthroline, tannic acid, and mercurials (mercury dichloride, Mersalyl acid, p-hydroxymercuribenzoate). Participates in mitochondrial genome maintenance, regulation of mitochondrial membrane potential and mitochondrial respiration.1 Publication

Kineticsi

The inhibitory constants (Ki) of these compounds are 1.3 um (UTP), 224 um (CTP), 276 um (GTP), and 181 um (ITP). CTP is the best substrate.1 Publication

Manual assertion based on experiment ini

  1. KM=1.19 mM for UTP (at 25 degrees Celsius)1 Publication
  2. KM=0.21 mM for CTP (at 25 degrees Celsius)1 Publication
  3. KM=2.5 mM for TTP (at 25 degrees Celsius)1 Publication
  4. KM=0.23 mM for GTP (at 25 degrees Celsius)1 Publication
  1. Vmax=30.7 µmol/min/g enzyme toward UTP (at 25 degrees Celsius)1 Publication
  2. Vmax=51.2 µmol/min/g enzyme toward CTP (at 25 degrees Celsius)1 Publication
  3. Vmax=8 µmol/min/g enzyme toward TTP (at 25 degrees Celsius)1 Publication
  4. Vmax=23.6 µmol/min/g enzyme toward GTP (at 25 degrees Celsius)1 Publication

GO - Molecular functioni

  • pyrimidine nucleotide transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  • mitochondrial genome maintenance Source: UniProtKB
  • mitochondrion organization Source: UniProtKB
  • pyrimidine nucleotide transport Source: UniProtKB
  • regulation of mitochondrial membrane potential Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi2.A.29.10.6. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 25 member 36
Gene namesi
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:25554. SLC25A36.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 27Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei41 – 57Helical; Name=2Sequence analysisAdd BLAST17
Transmembranei111 – 131Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei180 – 200Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei226 – 246Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei291 – 311Helical; Name=6Sequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi55186.
OpenTargetsiENSG00000114120.
PharmGKBiPA142670908.

Polymorphism and mutation databases

BioMutaiSLC25A36.
DMDMi74760768.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002917971 – 311Solute carrier family 25 member 36Add BLAST311

Proteomic databases

MaxQBiQ96CQ1.
PaxDbiQ96CQ1.
PeptideAtlasiQ96CQ1.
PRIDEiQ96CQ1.

PTM databases

iPTMnetiQ96CQ1.
PhosphoSitePlusiQ96CQ1.

Expressioni

Tissue specificityi

Expressed at moderate level.1 Publication

Gene expression databases

BgeeiENSG00000114120.
CleanExiHS_SLC25A36.
ExpressionAtlasiQ96CQ1. baseline and differential.
GenevisibleiQ96CQ1. HS.

Organism-specific databases

HPAiHPA027036.

Interactioni

Protein-protein interaction databases

BioGridi120484. 2 interactors.
IntActiQ96CQ1. 1 interactor.
MINTiMINT-1428592.
STRINGi9606.ENSP00000320688.

Structurei

3D structure databases

ProteinModelPortaliQ96CQ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 108Solcar 1PROSITE-ProRule annotationAdd BLAST105
Repeati116 – 203Solcar 2PROSITE-ProRule annotationAdd BLAST88
Repeati224 – 308Solcar 3PROSITE-ProRule annotationAdd BLAST85

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0757. Eukaryota.
ENOG410XS20. LUCA.
GeneTreeiENSGT00550000074670.
HOGENOMiHOG000188210.
HOVERGENiHBG059891.
InParanoidiQ96CQ1.
KOiK15116.
OMAiLNGIFDP.
OrthoDBiEOG091G0BCW.
PhylomeDBiQ96CQ1.
TreeFamiTF314220.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96CQ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQRDTLVHL FAGGCGGTVG AILTCPLEVV KTRLQSSSVT LYISEVQLNT
60 70 80 90 100
MAGASVNRVV SPGPLHCLKV ILEKEGPRSL FRGLGPNLVG VAPSRAIYFA
110 120 130 140 150
AYSNCKEKLN DVFDPDSTQV HMISAAMAGF TAITATNPIW LIKTRLQLDA
160 170 180 190 200
RNRGERRMGA FECVRKVYQT DGLKGFYRGM SASYAGISET VIHFVIYESI
210 220 230 240 250
KQKLLEYKTA STMENDEESV KEASDFVGMM LAAATSKTCA TTIAYPHEVV
260 270 280 290 300
RTRLREEGTK YRSFFQTLSL LVQEEGYGSL YRGLTTHLVR QIPNTAIMMA
310
TYELVVYLLN G
Length:311
Mass (Da):34,283
Last modified:December 1, 2001 - v1
Checksum:iD87DFDF7BC992D83
GO
Isoform 2 (identifier: Q96CQ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-132: FTA → HFL
     133-311: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:132
Mass (Da):14,046
Checksum:i4763D55F1DE496D2
GO
Isoform 3 (identifier: Q96CQ1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-248: Missing.

Note: No experimental confirmation available.
Show »
Length:310
Mass (Da):34,153
Checksum:iF0D02800320CF2B9
GO
Isoform 4 (identifier: Q96CQ1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-157: Missing.

Note: Gene prediction based on EST data.
Show »
Length:154
Mass (Da):17,358
Checksum:iC4CE38F1CB1F9B69
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti216D → G in BAA91715 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0355171 – 157Missing in isoform 4. CuratedAdd BLAST157
Alternative sequenceiVSP_026240130 – 132FTA → HFL in isoform 2. 1 Publication3
Alternative sequenceiVSP_026241133 – 311Missing in isoform 2. 1 PublicationAdd BLAST179
Alternative sequenceiVSP_026242248Missing in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001480 mRNA. Translation: BAA91715.1.
AC108727 Genomic DNA. No translation available.
AC132032 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79012.1.
CH471052 Genomic DNA. Translation: EAW79013.1.
BC014064 mRNA. Translation: AAH14064.1.
BC019859 mRNA. No translation available.
AL136803 mRNA. Translation: CAB66737.2.
CCDSiCCDS3114.1. [Q96CQ1-3]
CCDS46927.1. [Q96CQ1-1]
RefSeqiNP_001098117.1. NM_001104647.1. [Q96CQ1-1]
NP_060625.2. NM_018155.2. [Q96CQ1-3]
UniGeneiHs.144130.

Genome annotation databases

EnsembliENST00000324194; ENSP00000320688; ENSG00000114120. [Q96CQ1-1]
ENST00000446041; ENSP00000401938; ENSG00000114120. [Q96CQ1-3]
ENST00000502594; ENSP00000423319; ENSG00000114120. [Q96CQ1-2]
ENST00000631654; ENSP00000487839; ENSG00000114120. [Q96CQ1-2]
GeneIDi55186.
KEGGihsa:55186.
UCSCiuc003etr.3. human. [Q96CQ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001480 mRNA. Translation: BAA91715.1.
AC108727 Genomic DNA. No translation available.
AC132032 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79012.1.
CH471052 Genomic DNA. Translation: EAW79013.1.
BC014064 mRNA. Translation: AAH14064.1.
BC019859 mRNA. No translation available.
AL136803 mRNA. Translation: CAB66737.2.
CCDSiCCDS3114.1. [Q96CQ1-3]
CCDS46927.1. [Q96CQ1-1]
RefSeqiNP_001098117.1. NM_001104647.1. [Q96CQ1-1]
NP_060625.2. NM_018155.2. [Q96CQ1-3]
UniGeneiHs.144130.

3D structure databases

ProteinModelPortaliQ96CQ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120484. 2 interactors.
IntActiQ96CQ1. 1 interactor.
MINTiMINT-1428592.
STRINGi9606.ENSP00000320688.

Protein family/group databases

TCDBi2.A.29.10.6. the mitochondrial carrier (mc) family.

PTM databases

iPTMnetiQ96CQ1.
PhosphoSitePlusiQ96CQ1.

Polymorphism and mutation databases

BioMutaiSLC25A36.
DMDMi74760768.

Proteomic databases

MaxQBiQ96CQ1.
PaxDbiQ96CQ1.
PeptideAtlasiQ96CQ1.
PRIDEiQ96CQ1.

Protocols and materials databases

DNASUi55186.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324194; ENSP00000320688; ENSG00000114120. [Q96CQ1-1]
ENST00000446041; ENSP00000401938; ENSG00000114120. [Q96CQ1-3]
ENST00000502594; ENSP00000423319; ENSG00000114120. [Q96CQ1-2]
ENST00000631654; ENSP00000487839; ENSG00000114120. [Q96CQ1-2]
GeneIDi55186.
KEGGihsa:55186.
UCSCiuc003etr.3. human. [Q96CQ1-1]

Organism-specific databases

CTDi55186.
DisGeNETi55186.
GeneCardsiSLC25A36.
H-InvDBHIX0021205.
HGNCiHGNC:25554. SLC25A36.
HPAiHPA027036.
MIMi616149. gene.
neXtProtiNX_Q96CQ1.
OpenTargetsiENSG00000114120.
PharmGKBiPA142670908.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0757. Eukaryota.
ENOG410XS20. LUCA.
GeneTreeiENSGT00550000074670.
HOGENOMiHOG000188210.
HOVERGENiHBG059891.
InParanoidiQ96CQ1.
KOiK15116.
OMAiLNGIFDP.
OrthoDBiEOG091G0BCW.
PhylomeDBiQ96CQ1.
TreeFamiTF314220.

Miscellaneous databases

ChiTaRSiSLC25A36. human.
GenomeRNAii55186.
PROiQ96CQ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114120.
CleanExiHS_SLC25A36.
ExpressionAtlasiQ96CQ1. baseline and differential.
GenevisibleiQ96CQ1. HS.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS2536_HUMAN
AccessioniPrimary (citable) accession number: Q96CQ1
Secondary accession number(s): A8MYF7
, Q05CY1, Q9H0G8, Q9NVN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.