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Protein

EVI5-like protein

Gene

EVI5L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a GTPase-activating protein (GAP) with a broad specificity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei156 – 1561Arginine fingerBy similarity
Sitei197 – 1971Glutamine fingerBy similarity

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB
  • Rab GTPase binding Source: UniProtKB

GO - Biological processi

  • negative regulation of cilium assembly Source: UniProtKB
  • positive regulation of GTPase activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
EVI5-like protein
Alternative name(s):
Ecotropic viral integration site 5-like protein
Gene namesi
Name:EVI5L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:30464. EVI5L.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134905968.

Polymorphism and mutation databases

BioMutaiEVI5L.
DMDMi74731362.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 794794EVI5-like proteinPRO_0000263097Add
BLAST

Proteomic databases

MaxQBiQ96CN4.
PaxDbiQ96CN4.
PRIDEiQ96CN4.

PTM databases

PhosphoSiteiQ96CN4.

Expressioni

Gene expression databases

BgeeiQ96CN4.
CleanExiHS_EVI5L.
ExpressionAtlasiQ96CN4. baseline and differential.
GenevisibleiQ96CN4. HS.

Organism-specific databases

HPAiHPA043099.

Interactioni

Subunit structurei

May interact with RAB10.

Binary interactionsi

WithEntry#Exp.IntActNotes
NUDT18Q6ZVK83EBI-749523,EBI-740486

Protein-protein interaction databases

BioGridi125449. 9 interactions.
IntActiQ96CN4. 2 interactions.
STRINGi9606.ENSP00000445905.

Structurei

3D structure databases

ProteinModelPortaliQ96CN4.
SMRiQ96CN4. Positions 108-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini115 – 300186Rab-GAP TBCPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili358 – 44992Sequence AnalysisAdd
BLAST
Coiled coili569 – 709141Sequence AnalysisAdd
BLAST

Domaini

The arginine and glutamine fingers are critical for the GTPase-activating mechanism, they pull out Rab's 'switch 2' glutamine and insert in Rab's active site.By similarity

Sequence similaritiesi

Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5210.
GeneTreeiENSGT00750000117238.
HOGENOMiHOG000044859.
HOVERGENiHBG081486.
InParanoidiQ96CN4.
OMAiQVNQMEL.
OrthoDBiEOG7PS1DT.
PhylomeDBiQ96CN4.
TreeFamiTF317184.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96CN4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPTLSPDS SSQEALSAPT CSPTSDSENL SPDELELLAK LEEQNRLLEA
60 70 80 90 100
DSKSMRSMNG SRRNSGSSLV SSSSASSNLS HLEEDTWILW GRIANEWEEW
110 120 130 140 150
RRRKEKLLKE LIRKGIPHHF RAIVWQLLCS ATDMPVKNQY SELLKMSSPC
160 170 180 190 200
EKLIRRDIAR TYPEHEFFKG QDSLGQEVLF NVMKAYSLVD REVGYCQGSA
210 220 230 240 250
FIVGLLLMQM PEEEAFCVFV RLMQEYRLRE LFKPSMAELG LCIYQFEYML
260 270 280 290 300
QEQLPDLNTH FRSQSFHTSM YASSWFLTLF LTTFPLPVAT RVFDIFMYEG
310 320 330 340 350
LEIVFRVGLA LLQVNQAELM QLDMEGMSQY FQRVIPHQFD SCPDKLVLKA
360 370 380 390 400
YQVKYNPKKM KRLEKEYAAM KSKEMEEQIE IKRLRTENRL LKQRIETLEK
410 420 430 440 450
GQVTRAQEAE ENYVIKRELA VVRQQCSSAA EDLQKAQSTI RQLQEQQENP
460 470 480 490 500
RLTEDFVSHL ETELEQSRLR ETETLGALRE MQDKVLDMEK RNSSLPDENN
510 520 530 540 550
VAQLQEELKA LKVREGQAVA STRELKLQLQ ELSDTWQAHL ARGGRWKESP
560 570 580 590 600
RKLVVGELQD ELMSVRLREA QALAEGRELR QRVVELETQD HIHRNLLNRV
610 620 630 640 650
EAERAALQEK LQYLAAQNKG LQTQLSESRR KQAEAECKSK EEVMAVRLRE
660 670 680 690 700
ADSMAAVAEM RQRIAELEIQ REEGRIQGQL NHSDSSQYIR ELKDQIEELK
710 720 730 740 750
AEVRLLKGPP PFEDPLAFDG LSLARHLDED SLPSSDEELL GVGVGAALQD
760 770 780 790
ALYPLSPRDA RFFRRLERPA KDSEGSSDSD ADELAAPYSQ GLDN
Length:794
Mass (Da):91,376
Last modified:December 1, 2001 - v1
Checksum:i598B06DD2F098664
GO
Isoform 2 (identifier: Q96CN4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     400-400: K → KESAALADRLIQ

Note: No experimental confirmation available.
Show »
Length:805
Mass (Da):92,544
Checksum:i7A356B302C7CF2E8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei400 – 4001K → KESAALADRLIQ in isoform 2. 1 PublicationVSP_043456

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB449874 mRNA. Translation: BAH16617.1.
AC008812 Genomic DNA. No translation available.
AC010336 Genomic DNA. No translation available.
BC014111 mRNA. Translation: AAH14111.1.
CCDSiCCDS12188.1. [Q96CN4-1]
CCDS54209.1. [Q96CN4-2]
RefSeqiNP_001153416.1. NM_001159944.2. [Q96CN4-2]
NP_660288.1. NM_145245.4. [Q96CN4-1]
XP_005272515.1. XM_005272458.3. [Q96CN4-2]
UniGeneiHs.26870.

Genome annotation databases

EnsembliENST00000270530; ENSP00000270530; ENSG00000142459. [Q96CN4-1]
ENST00000538904; ENSP00000445905; ENSG00000142459. [Q96CN4-2]
GeneIDi115704.
KEGGihsa:115704.
UCSCiuc002min.3. human. [Q96CN4-1]
uc010xjz.2. human. [Q96CN4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB449874 mRNA. Translation: BAH16617.1.
AC008812 Genomic DNA. No translation available.
AC010336 Genomic DNA. No translation available.
BC014111 mRNA. Translation: AAH14111.1.
CCDSiCCDS12188.1. [Q96CN4-1]
CCDS54209.1. [Q96CN4-2]
RefSeqiNP_001153416.1. NM_001159944.2. [Q96CN4-2]
NP_660288.1. NM_145245.4. [Q96CN4-1]
XP_005272515.1. XM_005272458.3. [Q96CN4-2]
UniGeneiHs.26870.

3D structure databases

ProteinModelPortaliQ96CN4.
SMRiQ96CN4. Positions 108-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125449. 9 interactions.
IntActiQ96CN4. 2 interactions.
STRINGi9606.ENSP00000445905.

PTM databases

PhosphoSiteiQ96CN4.

Polymorphism and mutation databases

BioMutaiEVI5L.
DMDMi74731362.

Proteomic databases

MaxQBiQ96CN4.
PaxDbiQ96CN4.
PRIDEiQ96CN4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000270530; ENSP00000270530; ENSG00000142459. [Q96CN4-1]
ENST00000538904; ENSP00000445905; ENSG00000142459. [Q96CN4-2]
GeneIDi115704.
KEGGihsa:115704.
UCSCiuc002min.3. human. [Q96CN4-1]
uc010xjz.2. human. [Q96CN4-2]

Organism-specific databases

CTDi115704.
GeneCardsiGC19P007895.
H-InvDBHIX0174422.
HGNCiHGNC:30464. EVI5L.
HPAiHPA043099.
neXtProtiNX_Q96CN4.
PharmGKBiPA134905968.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5210.
GeneTreeiENSGT00750000117238.
HOGENOMiHOG000044859.
HOVERGENiHBG081486.
InParanoidiQ96CN4.
OMAiQVNQMEL.
OrthoDBiEOG7PS1DT.
PhylomeDBiQ96CN4.
TreeFamiTF317184.

Miscellaneous databases

GenomeRNAii115704.
NextBioi79654.
PROiQ96CN4.

Gene expression databases

BgeeiQ96CN4.
CleanExiHS_EVI5L.
ExpressionAtlasiQ96CN4. baseline and differential.
GenevisibleiQ96CN4. HS.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity."
    Ishibashi K., Kanno E., Itoh T., Fukuda M.
    Genes Cells 14:41-52(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  4. "Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity."
    Itoh T., Satoh M., Kanno E., Fukuda M.
    Genes Cells 11:1023-1037(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RAB10.
  5. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiEVI5L_HUMAN
AccessioniPrimary (citable) accession number: Q96CN4
Secondary accession number(s): B9A6I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.